检索结果-南通市图书馆

Substitutions of stem-loop subdomains in internal ribosome entry site of Senecavirus A:Impacts on rescue of sequence-modifying viruses

引用

Journal of Integrative Agriculture 2024年第7期23卷 2391-2406页

作者： Qianqian Wang Jie Wang Lei zhang Xiaoxiao Duan Lijie Zhu youming zhang Yan Li Fuxiao Liu College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China College of Veterinary Medicine Inner Mongolia Agricultural UniversityHuhhot 010018China Shandong New Hope Liuhe Group Co. Ltd.Qingdao 266100China Qingdao Center for Animal Disease Control&Prevention Qingdao 266199China State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China

Senecavirus A(SVA)has a positive-sense,single-stranded RNA genome.Its 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)that have been demonstrated to be well conserved.However,it is still unclear whether each stem-loop subdomain,such as a single stem or loop,is also highly conserved.To clarify this issue in the present study,a set of 29 SVA cDNA clones were constructed by site-directed mutagenesis(SDM)on the IRES.The SDM-modified scenarios included:(1)stem-formed complementary sequences exchanging with each other;(2)loop transversion;(3)loop transition;and(4)point mutations.All cDNA clones were separately transfected into cells for rescuing viable viruses,whereas only four SVAs of interest could be recovered,and were genetically stable during 20 passages.One progeny grew significantly slower than the other three did.The dual-luciferase reporter assay showed that none of the SDM-modified IRESes significantly inhibited the IRES activity.Our previous study indicated that a single motif from any of the ten stem structures,if completely mutated,would cause the failure of virus recovery.Interestingly,our present study revealed three stem structures,whose individual complementary sequences could exchange with each other to rescue sequence-modifying SVAs.Moreover,one apical loop was demonstrated to have the ability to tolerate its own full-length transition,also having no impact on the recovery of sequence-modifying SVA.The present study suggested that not every stem-loop structure was strictly conserved in its conformation,while the full-length IRES itself was well conserved.This provides a new research direction on interaction between the IRES and many factors.

关键词： SVA HCV IRES HCV-like IRES stem-loop structure cDNA clone virus rescue mutation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Logical stochastic resonance in a cross-bifurcation non-smooth system

引用

Chinese Physics B 2024年第3期33卷 659-667页

作者：张宇青雷佑铭 School of Mathematics and Statistics Northwestern Polytechnical UniversityXi'an 710072China Ministry of Industry and Information Technology Key Laboratory of Dynamics and Control of Complex Systems Northwestern Polytechnical UniversityXi'an 710072China

This paper investigates logical stochastic resonance(LSR)in a cross-bifurcation non-smooth system driven by Gaussian colored noise.In this system,a bifurcation parameter triggers a transition between monostability,bistability and tristability.By using Novikov's theorem and the unified colored noise approximation method,the approximate Fokker-Planck equation is obtained.Then we derive the generalized potential function and the transition rates to analyze the LSR phenomenon using numerical simulations.We simulate the logic operation of the system in the bistable and tristable regions respectively.We assess the impact of Gaussian colored noise on the LSR and discover that the reliability of the logic response depends on the noise strength and the bifurcation parameter.Furthermore,it is found that the bistable region has a more extensive parameter range to produce reliable logic operation compared with the tristable region,since the tristable region is more sensitive to noise than the bistable one.

关键词： logical stochastic resonance bifurcation mean first passage time

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A metamaterial-free fluid-flow cloak

引用

National Science Review 2022年第9期9卷 212-218页

作者： Fuyang Tay youming zhang Hongyi Xu Honghui Goh Yu Luo Baile zhang Division of Physics and Applied Physics School of Physical and Mathematical Sciences Nanyang Technological University School of Electrical and Electronic Engineering Nanyang Technological University Centre for Disruptive Photonic Technologies Nanyang Technological University

The model of ide al fluid flow around a cylindrical obstacle exhibits a long-established physical picture,where originally straight streamlines are deflected over the whole space by the obstacle.Inspired by transformation optics and metamaterials,recent theories have proposed the concept of fluid cloaking,which is able to recover the straight streamlines,as if the obstacle did not exist.However,such a cloak,similar to all previous transformation-optics-based devices,relies on complex metamaterials with inhomogeneous parameters and is difficult to implement.Here we deploy the theory of scattering cancellation and report on the experimental realization of a fluid-flow cloak without metamaterials.This clo ak is realized by engineering the geometry of the fluid channel,which effectively cancels the dipole-like scattering of the obstacle.The clo aking effect is demonstrated through the direct observation of recovered straight streamlines in the fluid flow.Our work sheds new light on conventional fluid control and may find application in microfluidic devices.

关键词： fluid-flow control invisibility cloaks metamaterials

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

From gene identifications to therapeutic targets for asthma:Focus on great potentials of TSLP,ORMDL3,and GSDMB

引用

Chinese Medical Journal Pulmonary and Critical Care Medicine 2023年第3期1卷 139-147页

作者： youming zhang National Heart and Lung Institute Imperial College LondonDovehouse StreetLondon SW36LYUK

Asthma is a chronic respiratory disease,and clinically,asthma exacerbations remain difficult to treat.The disease is caused by combinations of and interactions between genetic and environmental factors.Genomic and genetic approaches identified many novel genes to treat asthma and brought new insights into the disease.The products of the genes have functional roles in regulating physiological or pathophysiological processes in airway structural cells and immune system cells.Genetic factors also interact with environmental factors such as air pollutants,and bacterial and viral infections to trigger the disease.Thymic stromal lymphopoietin(TSLP),orosomucoid-like 3(ORMDL3),and gasdermin B(GSDMB)are three genes identified by genetic studies to have a great potential as therapeutic targets of asthma.TSLP is an important driver of type 2 inflammation.ORMDL3 mediates cell stress,sphingolipid synthesis,and viral and bacterial infections.GSDMB regulates cell pyroptosis through its N and C terminals and can bind sulfatides to influence inflammatory response.Investigating inhibitors or modulators for these pathways would bring a new landscape for therapeutics of asthma in future.

关键词： Asthma Thymic stromal lymphopoietin(TSLP) Orosomucoid-like 3(ORMDL3) Gasdermin B(GSDMB) Therapeutic targets

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tetradentate cyclometalated platinum complex enables high-performance near-infrared electroluminescence with excellent device stability

引用

Chinese Chemical Letters 2023年第2期34卷 511-514页

作者： Chengjun Wu youming zhang Jingsheng Miao Kai Li Weiguo Zhu Chuluo Yang Shenzhen Key Laboratory of New Information Display and Storage Materials Guangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and EngineeringShenzhen UniversityShenzhen 518055China Jiangsu Engineering Laboratory of Light-Electricity-Heat Energy-Converting Materials and Applications Changzhou UniversityChangzhou 213164China

Due to the high decay rate of the non-radiative transition of long wavelengths, the molecular design of efficient and stable near-infrared(NIR) electroluminescent materials remains a big challenge. Herein,a new tetradentate cyclometalated platinum(II) complex with an N∧C∧C∧N coordinated framework has been developed and used as a dopant for NIR organic light-emitting diodes(OLEDs). The complex exhibited a short-lived(0.5–1.5 μs) metal-to-ligand charge transfer(MLCT) excited state in doped and neat films. The resulting NIR OLEDs(λ_(EL)= 730 nm) achieved maximum external quantum efficiency(EQE_(max))of 5.2% and radiance of 74626 m W sr^(-1)m^(–2). Of note, the device exhibited excellent stability with operational lifetime of 119 h for LT_(90). This work demonstrated the great potential of tetradentate platinum(II)complexes in the field of NIR OLEDs.

关键词： Near-infrared emitter Phosphorescence Platinum complex OLED Operational lifetime

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

引用

Neural Regeneration Research 2025年第3期20卷 821-835页

作者： Jing Wang Bing zhang Lanfang Li Xiaomei Tang Jinyu Zeng Yige Song Chao Xu Kai Zhao Guoqiang Liu youming Lu Xinyan Li Kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive impairment.However,the mechanism underlying this neurogenesis impairment remains unknown.In this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain injury.Our results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and spines.Mice with repetitive traumatic brain injuryalso showed deficits in spatial memory retrieval.Moreover,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was downregulated.An inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological function.These findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fabricating a novel supramolecular light-activated platform based on internal-driven forces induced by the UV-light

引用

Chinese Chemical Letters 2022年第12期33卷 5065-5068页

作者： Xiaoni Qi Weichun Li Bingbing Shi youming zhang Hong Yao Qi Lin Taibao Wei Key Laboratory of Eco-Environmental Polymer Materials of Gansu Province College of Chemistry and Chemical EngineeringNorthwest Normal UniversityLanzhou 730070China

Recently,exploiting a novel supramolecular fabrication pathway have drawn great attention.To this endeavor,we firstly designed and reported an original light-activated platform based on the internal-driven forces of macrocyclic host by hiring the pillar[5]arene as the host molecule(H)and phenazine derivatives acting as an energetic guest molecule(G).Surprisingly,after adding the H solution into G system,the intensive fluorescence emission of the G molecule rapidly decreased under the irradiation of the UV-light(254 nm)until absolutely quenching.Delightfully,different from the traditional supramolecular host-guest interaction,the fluorescent emission of G molecule could be recovered after irradiating under the nature light.In view of this interesting observations,the interaction mechanism was carefully investigated by a series of characterizations.Those results suggested that the G molecule was easily threaded into the macrocyclic cavity(H)under the internal-driven forces induced by the UV-light irradiation,forming a 1:1 host-guest complex.Moreover,taking advantage of this especial feature,the light-activated platform of host-guest complex was further applied for ink-free light-driven printing materials,exhibiting great potential in the real application.

关键词： Light-activated platform Pillararene Phenazine derivatives Internal-driven forces UV-light

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The emerging role of recombineering in microbiology

引用

Engineering Microbiology 2023年第3期3卷 75-83页

作者： Ruijuan Li Aiying Li youming zhang Jun Fu State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China Chinese Academy of Sciences(CAS)Key Laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenChina

Recombineering is a valuable technique for generating recombinant DNA in vivo,primarily in bacterial cells,and is based on homologous recombination using phage-encoded homologous recombinases,such as Red βγ from the lambda phage and RecET from the Rac prophage.The recombineering technique can efficiently mediate homol-ogous recombination using short homologous arms(∼50 bp)and is unlimited by the size of the DNA molecules or positions of restriction sites.In this review,we summarize characteristics of recombinases,mechanism of recombineering,and advances in recombineering for DNA manipulation in Escherichia coli and other bacteria.Furthermore,the broad applications of recombineering for mining new bioactive microbial natural products,and for viral mutagenesis,phage genome engineering,and understanding bacterial metabolism are also reviewed.

关键词： Recombineering Natural products Viral mutagenesis Phage genome engineering Bacterial metabolism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer’s disease mice

引用

Protein & Cell 2024年第4期15卷 261-284页

作者： He-Zhou Huang Wen-Qing Ai Na Wei Ling-Shuang Zhu Zhi-Qiang Liu Chao-Wen Zhou Man-Fei Deng Wen-Tao zhang Jia-Chen zhang Chun-Qing Yang Ya-Zhuo Hu Zhi-Tao Han Hong-Hong zhang Jian-Jun Jia Jing Wang Fang-Fang Liu Ke Li Qi Xu Mei Yuan Hengye Man Ziyuan Guo youming Lu Kai Shu Ling-Qiang Zhu Dan Liu Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Medical Genetics School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pathology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450002China Department of Pathology School of Basic MedicineZhengzhou UniversityZhengzhou 450002China The Second Affiliated Hospital Department of NeurologyHengyang Medical SchoolUniversity of South ChinaHengyang 421001China Beijing Key Laboratory of Aging and Geriatrics National Clinical Research Center for Geriatric DiseaseInstitute of GeriatricsChinese PLA General Hospital and Chinese PLA Medical AcademyBeijing 100853China Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Neurology Union HospitalHuazhong University of Science and TechnologyWuhan 430022China Department of Biology Boston UniversityBostonMA 02215USA Center for Stem Cell and Organoid Medicine(CuSTOM) Division of Developmental BiologyCincinnati Children’s Hospital Medical CenterCincinnatiOH 45229USA

Sporadic or late-onset Alzheimer’s disease(LOAD)accounts for more than 95%of Alzheimer’s disease(AD)cases without any family history.Although genome-wide association studies have identified associated risk genes and loci for LOAD,numerous studies suggest that many adverse environmental factors,such as social isolation,are associated with an increased risk of dementia.However,the underlying mechanisms of social isolation in AD progression remain elusive.In the current study,we found that 7 days of social isolation could trigger pattern separation impairments and presynaptic abnormalities of the mossy fibre-CA3 circuit in AD mice.We also revealed that social isolation disrupted histone acetylation and resulted in the downregulation of 2 dentate gyrus(DG)-enriched miRNAs,which simultaneously target reticulon 3(RTN3),an endoplasmic reticulum protein that aggregates in presynaptic regions to disturb the formation of functional mossy fibre boutons(MFBs)by recruiting multiple mitochondrial and vesicle-related proteins.Interestingly,the aggregation of RTN3 also recruits the PP2A B subunits to suppress PP2A activity and induce tau hyperphosphorylation,which,in turn,further elevates RTN3 and forms a vicious cycle.Finally,using an artificial intelligence-assisted molecular docking approach,we determined that senktide,a selective agonist of neurokinin3 receptors(NK3R),could reduce the binding of RTN3 with its partners.Moreover,application of senktide in vivo effectively restored DG circuit disorders in socially isolated AD mice.Taken together,our findings not only demonstrate the epigenetic regulatory mechanism underlying mossy fibre synaptic disorders orchestrated by social isolation and tau pathology but also reveal a novel potential therapeutic strategy for AD.

关键词： Alzheimer’s disease memory impairment synaptic disorder tau pathology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Road map for, and technical challenges of, carbon-nanotube integrated circuit technology

引用

National Science Review 2024年第3期11卷 14-16页

作者： Jia Si Panpan zhang Zhiyong zhang Key Laboratory for the Physics and Chemistry of Nanodevices and Center for Carbon-based Electronics School of Electronics Peking University State Key Laboratory of Information Photonics and Optical Communications Beijing University of Posts and Telecommunications

The past decade has witnessed an explosion of research on carbon-nanotube(CNT) electronics due to their outstanding properties, including an ultrathin body, a high carrier mobility and a high saturation velocity.

关键词： TRANSISTORS

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：