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Oxidative stress,the blood-brain barrier and neurodegenerative diseases:The critical beneficial role of dietary antioxidants

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Acta Pharmaceutica Sinica B 2023年第10期13卷 3988-4024页

作者： Jin Feng youle zheng Mingyue Guo Irma Ares Marta Martínez Bernardo Lopez-Torres María-Rosa Martínez-Larran aga Xu Wang Arturo Anadon María-Aranzazu Martínez National Reference Laboratory of Veterinary Drug Residues(HZAU)and MAO Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural UniversityWuhan 430070China MAO Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Huazhong Agricultural UniversityWuhan 430070China Department of Pharmacology and Toxicology Faculty of Veterinary MedicineUniversidad Complutense de Madrid(UCM)And Research Institute Hospital 12 de Octubre(i+12)Madrid 28040Spain

In recent years,growing awareness of the role of oxidative stress in brain health has prompted antioxidants,especially dietary antioxidants,to receive growing attention as possible treatments strategies for patients with neurodegenerative diseases(NDs).The most widely studied dietary antioxidants include active substances such as vitamins,carotenoids,flavonoids and polyphenols.Dietary antioxidants are found in usually consumed foods such as fresh fruits,vegetables,nuts and oils and are gaining popularity due to recently growing awareness of their potential for preventive and protective agents against NDs,as well as their abundant natural sources,generally non-toxic nature,and ease of long-term consumption.This review article examines the role of oxidative stress in the development of NDs,explores the‘two-sidedness’of the blood-brain barrier(BBB)as a protective barrier to the nervous system and an impeding barrier to the use of antioxidants as drug medicinal products and/or dietary antioxidants supplements for prevention and therapy and reviews the BBB permeability of common dietary antioxidant suplements and their potential efficacy in the prevention and treatment of NDs.Finally,current challenges and future directions for the prevention and treatment of NDs using dietary antioxidants are discussed,and useful information on the prevention and treatment of NDs is provided.

关键词： Dietary antioxidant supplements Oxidative stress Blood-brain barrier Neurodegenerative diseases Vitamins Carotenoids Flavonoids Polyphenols

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Evaluation of an indirect ELISA using recombinant arginine kinase for serodiagnosis of Psoroptesovis var.cuniculi infestation in rabbits

Evaluation of an indirect ELISA using recombinant arginine k...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论...

作者： Jiang Gu youle zheng Guangyou Yang Yue Xie Xiaobin Gu Department of Parasitology College of Veterinary MedicineSichuan Agricultural University

Psoroptesovis var.cuniculi is a common ectoparasite of the wild and domestic rabbits worldwide,which causes significant economic losses in commercial rabbit breeding.In China,the diagnosis of rabbits infested with P.ovis var.cuniculi currently relies on detection of clinical signs and Psoroptes mites in skin scrapings by microscopy examination.However,this method is not very efficient in detection of the low mite loads and/or sub-clinical infections.In the present study,we cloned and expressed an arginine kinase homologue gene from P.ovis var.cuniculi(Poc-AK)and used its recombinant protein rPoc-AK to develop an indirect enzyme-linked immunosorbent assay(iELISA) method for diagnosis of P.ovis var.cuniculi infestations in rabbits.The results showed that the rPoc-AK protein was～61 kDa and had no signal peptide.The rPoc-AK-based iELISA achieved a 94.4% sensitivity and a 88.2% specificity,and was able to detect P.ovis var.cuniculi infections as early as the 1 st week post-infection,prior to the appearance of clinical signs.Further field study showed 24.94%(66.3/266) clinically normal rabbits were seropositive with the highest and lowest seropositive rates for California(35.71%) and Belgian(15.14%),respectively.These results suggested that the rPoc-AK has potential as a diagnostic antigen for early P.ovis mite infestation in rabbits.

关键词： Psoroptesovis var.cuniculi arginine kinase indirect ELISA early serodiagnosis rabbit

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Whether gut symbionts of nematode parasites can mediate antihelminthic resistance or not:novel insights from the giant panda roundworm B.schroederi

Whether gut symbionts of nematode parasites can mediate anti...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论...

作者： Yue Xie Xuan Zhou Yunjian Liu Lu Wang youle zheng Yingxin Li XiaobinGu Guangyou Yang Department of Parasitology College of Veterinary MedicineSichuanAgricultural University Institute of Animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural University

Antihelminthic resistance is a serious concern worldwide,whose mechanisms have been attributed to evolutionary changes in parasite genomes such as alteration of drug target sites,up-regulation of degrading enzymes and enhancement of drug excretion.Here,we describe a previously unknown mechanism of antihelminthic resistance:gut symbionts of the giant panda roundworm parasite B.schroederi would enhance resistance to a wide-spectrum antihelminthic drug ivermectin.We documented that the gut symbiont bacteria Burkholderia sp.(called BS-B)and Citrobacter sp.(called BS-C) play key roles in the degradation of ivermectin.Comparative genomics revealed that compared to other congeneric species,BS-B had higher expressions of genes encoding glutathione S-transferases(GSTs) and multi-functional oxidases(MFOs),while BS-C had higher expressions of genes encoding carboxylesterase(CarEs) and cytochromes P450(P450 s).The transcriptions of these genes were also significantly up-regulated when ivermectin presented in cultures of BS-B and BS-C in vitro.Moreover,the ivermectin-sensitive B.schroederi inoculated with isolated BS-B and BS-C conferred ivermectin resistance,confirming the key roles of BS-B and BS-C in ivennectin resistance of B.schroederi.Taken together,our findings suggest possibility that the gut symbiont mediates antihelminthic resistance and also imply a more widely relevant drug resistance mechanism than previously recognized.

关键词： Gut symbiotic bacteria antihelminthic resistance ivermectin Burkholderia Citrobacter roundworms

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Molecular identification and characterization of Ancylostroma hookworms from stray and sheltered dogs of Sichuan province,China

Molecular identification and characterization of Ancylostrom...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论...

作者： Yue Xie Zhiyu Wang Yunjian Liu Lu Wang youle zheng Xuan Zhou Xiaobin Gu Guangyou Yang Department of Parasitology College of Veterinary MedicineSichuanAgricultural University Institute of Animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural University

Canine hookworm infections are endemic worldwide and pose major threats to public health.Stray and sheltered dogs are important reservoir hosts of such worms.However,only few studies on the prevalence of hookworm infections in stray and sheltered dogs are available in China so far.The present study was conducted to investigate the prevalence of intestinal hookworms in the stray and sheltered dogs in Sichuan Province,China,from March 2017 to February 2018.A total of 329 dogs from five locations including Wenjiang,Shuangliu,Leshan,Ya’an and Xichang of Sichuan were included and subjected to faecal examination for the presence of hookworm eggs using light microscopy and further species-level identification by analyses of nuclear internal transcribed spacers(ITS) and mitochondrial cytochrome c oxidase subunit 1(cox 1)genes.An overall prevalence of 13.1%(43/329) of hookworms was found among the stray and sheltered dogs,with the highest prevalence(55.8%) in Leshan,followed by lower values in Ya’an(12.2%),Wenjiang 7.8%),Leshan(6.9%) and Xichang(0%).The prevalence among stray dogs was significantly higher than that among shelter dogs(p<0.05).No significant differences were observed between genders or ages of these dogs.Further sequence analysis showed that Ancylostomaceylanicum was dominant species and Ancylostoma caninum was another species among these hookworm-positive samples.These results suggests high prevalences of A.ceylanicum and A.caninum in stray and sheltered dogs in Sichuan of China and indicates the need for control programs to minimize the risk of transmission of these two zoonotic hookworms in these areas because of these reservoir hosts living in close proximity to humans.

关键词： Stray and sheltered dog Ancytostroma hookworms zoonosis prevalence Sichuan

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Haemoglobin-based seroprevalence of Ascaris suum in fattening pigs in Sichuan province,China

Haemoglobin-based seroprevalence of Ascaris suum in fattenin...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论...

作者： youle zheng Yue Xie MI Qazi Yuhang Chen Xiaobin Gu Peter Geldhof Robin B.Gasser Tao Wang College of Veterinary Medicine Sichuan Agricultural University Laboratory of Parasitology Department of VirologyParasitology and ImmunologyFaculty of Veterinary MedicineGhent University Department of Veterinary Biosciences Melbourne Veterinary Schoolthe University of Melbourne

Ascaris suum,a soil-transmitted pig nematode,affects porcine industry worldwide due to poor production outcomes(reduced weight gain) and increased post-slaughter condemnation rate.To provide the valuable information regarding present situation of A.suum infection in China,a recently developed serological method for the detection of antibodies against A.suum haemoglobin(As-Hb) was used.This method has proven to achieve a higher sensitivity than that of traditional faecal flotation technique and has been applied with a considerable success in fattening pigs and nursery piglets in other countries.Utilizing the AsHb-based ELISA method,we examined A.suum exposure levels in 15 farms,of which eight were recorded historically as epidemiological data-deficient areas in the Sichuan province,China.Our results showed that among 524 finishing pigs,53.8% were determined to be infected with A.suum,with the highest infection rate(78.8%) observed in Dazhou and the lowest(0%) in Liangshan.Further,the seroprevalence of A.suum in backyard raised and intensively reared pigs was close,i.e.,53.0%and 54.0%,respectively.These numbers mirrored a severe epidemic status of A.suum infection in fattening pigs in Sichuan,and also stressedthe importance of monitoring the A.suum exposure levels in pig farms of China.Furthermore,theapplication of AsHb-based ELISA illustrated the importance of this serological test which could offer pig farmers and veterinarians an easier and more sensitive way to assess the overall prevalence of A.suum on pig farms and improve the farm management and hygiene practices.

关键词： Ascaris suum pigs As-Hb-based ELISA seroprevalence

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合并病理性骨折的手部单发内生软骨瘤疗效影响因素分析

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中国修复重建外科杂志 2014年第7期28卷 840-843页

作者：郑炜熊革李淳张友乐北京积水潭医院手外科北京100035

目的探讨合并病理性骨折的手部单发内生软骨瘤疗效影响因素。方法回顾性分析2010年3月-2013年6月符合选择标准纳入研究的65例合并病理性骨折的手部单发内生软骨瘤患者临床资料。男26例,女39例;年龄17~63岁,平均36岁。左手32例,右手33例... 详细信息

目的探讨合并病理性骨折的手部单发内生软骨瘤疗效影响因素。方法回顾性分析2010年3月-2013年6月符合选择标准纳入研究的65例合并病理性骨折的手部单发内生软骨瘤患者临床资料。男26例,女39例;年龄17~63岁,平均36岁。左手32例,右手33例。肿瘤部位:掌骨18例;指骨47例,其中近节30例、中节9例、末节8例。手术距骨折时间2 d^2年,平均56 d。患者均采用肿瘤刮除、植骨治疗。术后计算患侧与健侧手指总主动活动度的比值评价患指功能,记录患指恢复工作时间,采用多元线性回归方法分析疗效影响因素。结果术后65例均获随访,随访时间5~44个月,平均24个月。术后切口均Ⅰ期愈合,无感染、再骨折、肿瘤复发等发生。X线片复查示骨折均愈合,愈合时间5~10周,平均6.5周;植骨融合良好。末次随访时,手指总主动活动度比值为0.46~1.00,平均0.90。患指恢复工作时间为92~180 d,平均130 d。多元线性回归分析结果显示,是否应用内固定物是影响手指总主动活动度比值的影响因素(P=0.013),肿瘤部位、是否应用内固定物以及手术距骨折时间是影响患指恢复工作时间的影响因素(P<0.05)。结论对于伴病理性骨折的手部单发内生软骨瘤,建议选择早期肿瘤刮除并一期骨折复位钢板、螺钉内固定治疗,以获满意疗效。

关键词：内生软骨瘤病理性骨折手术疗效影响因素

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硒化低聚氨基多糖对免疫抑制小鼠免疫器官及肠紧密连接蛋白表达的影响

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食品科学 2019年第21期40卷 171-176页

作者：汤震顾丽霞相兴伟闻正顺曲有乐郑斌宋厚辉浙江海洋大学食品与医药学院浙江舟山316022 浙江省海洋开发研究院浙江舟山316021 浙江农林大学动物科技学院浙江杭州311300

本实验旨在研究硒化低聚氨基多糖(low-molecular-mass seleno-aminopolysaccharide,LSA)对免疫抑制小鼠组织形态、回肠闭合小环蛋白-1(zonula occludens-1,ZO-1)及闭锁蛋白(Occludin)表达的影响。通过腹腔注射环磷酰胺构建免疫抑制模型,测定脏器指数,采用苏木精-伊红染色观察脾脏与空肠组织病理形态,利用反转录实时荧光定量聚合酶链式反应分析ZO-1、Occludin mRNA的表达水平。结果表明:LSA处理显著缓解了环磷酰胺对小鼠免疫器官的抑制作用(P<0.05);与环磷酰胺模型组比较,0.6、0.9 mg/(kg mb·d) LSA组小鼠脾脏结构较清晰,红髓和白髓界限较明显,白髓面积增大,空肠绒毛形态与环磷酰胺模型组相比较为整齐;0.6 mg/(kg mb·d)LSA组的ZO-1、Occludin m RNA表达量显著升高(P<0.05)。综上,LSA具有一定的免疫调节功能及肠道保护作用。

关键词：硒化低聚氨基多糖免疫抑制闭合小环蛋白-1 闭锁蛋白

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硒化低聚氨基多糖对RAW264.7细胞的免疫调节作用

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食品科学 2018年第13期39卷 198-204页

作者：顾丽霞郑斌相兴伟闻正顺周宇芳马剑茵曲有乐浙江海洋大学食品与医药学院浙江舟山316022 浙江省海洋开发研究院浙江舟山316021

本实验旨在研究硒化低聚氨基多糖对小鼠巨噬细胞RAW264.7免疫功能的影响。用噻唑蓝比色法分别考察硒化低聚氨基多糖、Na2Se O3对巨噬细胞RAW264.7增殖能力的影响,并观察巨噬细胞形态的变化。通过中性红法、酶联免疫吸附测定法及实时荧... 详细信息

本实验旨在研究硒化低聚氨基多糖对小鼠巨噬细胞RAW264.7免疫功能的影响。用噻唑蓝比色法分别考察硒化低聚氨基多糖、Na2Se O3对巨噬细胞RAW264.7增殖能力的影响,并观察巨噬细胞形态的变化。通过中性红法、酶联免疫吸附测定法及实时荧光定量反转录聚合酶链式反应法分别检测巨噬细胞的吞噬能力、肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、白介素(interleukin,IL)-6及IL-10细胞因子的分泌量及其m RNA表达水平。结果表明:硒质量浓度为100~1 000μg/L时,硒化低聚氨基多糖对细胞的相对增殖率无显著影响;而Na2Se O3的硒质量浓度超过200μg/L即对巨噬细胞RAW264.7产生细胞毒性作用。硒化低聚氨基多糖能增强巨噬细胞吞噬活性,显著提高RAW264.7细胞TNF-α、IL-6及IL-10分泌量及其m RNA表达水平,且效果比Na2Se O3处理组、低聚氨基多糖处理组及Na2Se O3+低聚氨基多糖复合添加组好。实验结果提示硒化低聚氨基多糖对小鼠巨噬细胞RAW264.7具有一定的免疫调节作用。

关键词：硒硒化低聚氨基多糖巨噬细胞免疫调节

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绵羊痒螨巨噬细胞迁移抑制因子重组蛋白对兔外周血单个核细胞Th1/Th2型和Th17/Treg型特征因子表达的影响

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畜牧兽医学报 2020年第3期51卷 594-601页

作者：郑友乐陈宇航谢跃杨光友何冉古小彬四川农业大学动物医学院动物寄生虫病研究中心成都611130

为了初步探讨绵羊痒螨巨噬细胞迁移抑制因子(PoMIF)对健康新西兰兔外周血单个核细胞(PBMC)中Th1/Th2和Th17/Treg细胞平衡的变化。采用RT-PCR从绵羊痒螨总RNA中扩增得到MIF全长基因,经原核表达、纯化重组PoMIF(rPoMIF)蛋白,并分析其氧化... 详细信息

为了初步探讨绵羊痒螨巨噬细胞迁移抑制因子(PoMIF)对健康新西兰兔外周血单个核细胞(PBMC)中Th1/Th2和Th17/Treg细胞平衡的变化。采用RT-PCR从绵羊痒螨总RNA中扩增得到MIF全长基因,经原核表达、纯化重组PoMIF(rPoMIF)蛋白,并分析其氧化还原酶和互变异构酶活性。筛选与健康新西兰兔PBMC孵育的最佳rPoMIF浓度,且用最佳浓度rPoMIF(0.2μg·mL^-1)与PBMC共同孵育0、1、6、12、24、36 h后收集细胞,用荧光定量PCR检测其Th1/Th2/Th17/Treg细胞相对应的特征性转录因子T-bet/GATA-3/RORc/Foxp3和特征性细胞因子IFN-γ/IL-4/IL^-17A/IL^-10 mRNA表达变化。结果表明:PoMIF全长363 bp,rPoMIF大小为32 ku(含pET32a标签蛋白19 ku),且具有互变异构酶活性;rPoMIF刺激后PBMC中Th1细胞的T-bet和IFN-γ先下降后上升,Th2细胞的GATA-3和IL-4均呈下降趋势,且T-bet/GATA-3和IFN-γ/IL-4比值在12、24和36 h均升高;Th17细胞的RORc和IL^-17A在各时间点下降,而Treg细胞的Foxp3和IL^-10在各时间点升高,且RORc/Foxp3和IL^-17A/IL^-10比值在各时间点均变低。rPoMIF可造成家兔外周血单个核细胞的Th1/Th2和Th17/Treg平衡分别向Th1和Treg偏移。

关键词：绵羊痒螨兔巨噬细胞迁移抑制因子外周血单个核细胞 Th1/Th2平衡 Th17/Treg平衡

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喙肱韧带紧缩联合斜方肌移位重建肩外展功能的疗效评价

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骨科临床与研究杂志 2017年第1期2卷 11-16页

作者：李文军张友乐陈山林胡琪郑炜杨辰田文田光磊顾玉东北京积水潭医院手外科北京100035 上海复旦大学附属华山医院手外科

目的评价喙肱韧带紧缩联合斜方肌移位与异体肌腱移植重建肩外展功能的临床疗效。方法回顾分析2010年6月至2014年5月北京积水潭医院手外科采用复位半脱位肩关节并紧缩喙肱韧带,同时联合斜方肌移位与异体肌腱移植止点下移的方法重建肩外... 详细信息

目的评价喙肱韧带紧缩联合斜方肌移位与异体肌腱移植重建肩外展功能的临床疗效。方法回顾分析2010年6月至2014年5月北京积水潭医院手外科采用复位半脱位肩关节并紧缩喙肱韧带,同时联合斜方肌移位与异体肌腱移植止点下移的方法重建肩外展功能32例。术后采用顾玉东关于臂丛神经损伤术后各关节功能评定标准、快速DASH评分(q DASH)和疼痛视觉模拟评分(VAS)来评价肩外展功能,同时进行患者满意度调查。结果其中男28例,女4例,平均年龄25.0(11~47)岁。左侧17例,右侧15例。臂丛神经上中干根性撕脱伤30例,颈椎骨折合并神经损伤2例。32例患者均得到了随访,平均随访2.2(1.6~3.5)年。依据顾玉东标准,术后肩外展功能5例(15.6%)优(外展≥90°);25例(78.1%)良(90°>外展≥60°);2例(6.3%)患者手术后提前自行去除外固定架,导致缝合肌腱断裂,施行了二次手术,最终外展功能达到中(60°>外展≥30°)。VAS评分术前为7.2±0.9,术后为2.5±0.6(P<0.01);q DASH评分术前为68.9±10.7,术后为34.2±8.2(P<0.01);患者主观评价满意28例(87.5%);不满意4例(12.5%);32例患者术后获得了平均35°(30°~40°)的肩关节旋前功能。结论喙肱韧带紧缩联合斜方肌移位与异体肌腱移植止点下移重建肩外展功能,方法简单实用且有效,具有一定的临床推广价值。

关键词：肩关节臂丛创伤和损伤喙肱韧带斜方肌

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