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AIEgens in cell-based multiplex fluorescence imaging

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Science China Chemistry 2019年第10期62卷 1312-1332页

作者： Yabin Zhou Jin Hua Ben Zhong tang youhong tang Faculty of Biological Engineering Sichuan University of Science and EngineeringYibin 644000China Institute for NanoScale Science and Technology Medical Device Research InstituteCollege of Science and EngineeringFlinders UniversityTonsleySouth Australia 5042Australia Discipline of Medical Biochemistry College of Medicine and Public HealthFlinders UniversityBedford ParkSouth Australia 5042Australia Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and ReconstructionDivision of Biomedical EngineeringThe Hong Kong University of Science and TechnologyHong KongChina

Fluorescence imaging is an important branch of *** is non-invasive and provides superior spatial and temporal resolution during the real-time monitoring of biological samples of *** the spatial resolution limit of optical microscopes is about 200 nm,due to the diffraction limit,with the application of super-resolution fluorescence microscopy technologies this limit has been pushed below 30 *** makes it feasible to visualize biological structures in subcellular levels and to monitor subcellular biological processes in real ***,due to the complexity of the biological structure and components within cells,simultaneous staining and monitoring multiple intracellular components with different coloured fluorophores is often needed during multiplex imaging,to better understand biological ***-induced emission luminogens(AIEgen)and AIEgen based nanoparticles(NPs)have presented many advantages in fluorescence imaging,with strong potential for biological science and *** this review,we focus on the advantages of AIEgen and AIEgen NP in cell-based fluorescence imaging,and the latest advances of AIEgens in cell-based multiplex imaging are summarized and *** future perspectives are proposed.

关键词： aggregation induced emission cell imaging multiplex

Preparation of Silver Nanowires via a Rapid,Scalable and Green Pathway

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Journal of Materials Science & Technology 2015年第1期31卷 16-22页

作者： Cheng Yang youhong tang Zijin Su Zhexu Zhang Cheng Fang Division of Energy and Environment Graduate School at Shenzhen Tsinghua University Centre for Nanoscale Science and Technology School of Computer Science Engineering and MathematicsFlinders University Centre for Environmental Risk Assessment & Remediation University of South Australia

Rapid synthesis of silver nanowires(Ag NWs) with high quality and a broad processing window is challenging because of the low selectivity of the formation of multiply twinned particles at the nucleation stage for subsequent Ag NWs *** we report a systematic study of the water-involved heterogeneous nucleation of Ag NWs with high rate(less than 20 min) in a simple and scalable preparation *** glycerol as a reducing agent and a solvent with a high boiling point,the reaction is rapidly heated to 210 ℃ in air to synthesize Ag NWs with a very high yield in gram *** is noted that the addition of a small dose of water plays a key role for obtaining highly pure Ag NWs in high yield,and the optimal water/glycerol ratio is0.25%.After investigating a series of forming factors including reaction temperature and dose of catalysts,the formation kinetics and mechanism of the Ag NWs are *** to other preparation methods,our strategy is simple and *** Ag NWs show a strong Raman enhancement effect for organic molecules on their surface.

关键词： Silver nanowires Synthesis kinetics Surface enhanc

Synthetic fluorescent probes to apprehend calcium signalling in lipid droplet accumulation in microalgae——an updated review

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Science China Chemistry 2020年第3期63卷 308-324页

作者： AHM Mohsinul Reza Javad Tavakoli Yabin Zhou Jianguang Qin youhong tang College of Science and Engineering Flinders UniversityAdelaideSA 5001Australia Institute for NanoScale Science and Technology College of Science and EngineeringFlinders UniversityAdelaideSA 5001Australia

Lipid bodies are dynamic organelles of photosynthetic microalgae that can be used as the third generation resources for biofuel *** of lipids can be influenced by different signalling *** of these processes can provide useful information about the fate and associated roles of lipid molecules in different biological *** photosynthetic organisms,however,studies of calcium ediated lipid biosynthesis is bottlenecked due to the limitation of proper and efficient technologies,which also include visualisation ***,most studies to visualise lipid droplets in vivo have used traditional dyes,and proper visualisation of lipid drops is hindered by dye-specific *** hurdle could be overcome by using recently developed aggregation-induced emission *** review reveals current knowledge gaps in the studies of lipid drops and calcium ions in microalgae,as calcium signaling is important secondary messenger to detect a wide variety of environmental stimuli in plant and animal *** obtain insight into the mechanisms of these processes,the merits and demerits of currently available visualisation techniques for lipid drops and calcium are also ***,opportunities and possibilities are proposed to recommend further improvement of techniques for detecting the role of calcium during lipid formation in microalgae for biofuel production.

关键词： aggregation-induced emission calcium lipid droplets microalgae traditional fluorophores

Aggregation-induced Emission Fluorogen as Mammalian Cell Cytoplasmic Tracker with Long Retention Time and High Photo-stability

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Chemical Research in Chinese Universities 2021年第1期37卷 110-115页

作者： ZHOU Yabin HUA Jin ZHANG Hong-ping tang youhong School of Biological Engineering Sichuan University of Science and Engineering(SUSE)Yibin644000P.R.China Institute for NanoScale Science and Technology College of Science and EngineeringFlinders UniversityAdelaideSouth Australia5042Australia College of Medicine and Public Health Flinders UniversityAdelaideSouth Australia5064Australia School of Materials Science and Engineering Southwest University of Science and TechnologyMianyang621010P.R.China

Fluorescent probes used for cell imaging are powerful tools in cell-based assays and *** this study,we exhibited a water-soluble aggregation-induced emission fluorogen(AIEgen),BSPO-TPE,specifically stained cytoplasm in live cells and had an excellent photostability when compared to that of two widely used commercial fluorescent *** long cytoplasm retention time of BSPO-TPE demonstrated its suitability as a live cell cytoplasm tracker.

关键词： Aggregation-induced emission(AIE) BSPO-TPE Cytoplasmic tracker

Strategies in boosting photosensitization for biomedical applications

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Science China Chemistry 2022年第4期65卷 647-649页

作者： Ming Chen Ryan T.K.Kwok youhong tang Dan Ding College of Chemistry and Materials Science Jinan UniversityGuangzhou 510632China Frontiers Science Center for Cell Responses State Key Laboratory of Medicinal Chemical BiologyKey Laboratory of Bioactive MaterialsMinistry of EducationCollege of Life SciencesNankai UniversityTianjin 300071China Department of Nephrology Huai’an Hospital Affiliated to Xuzhou Medical College and Huai’an Second HospitalHuai’an 223002China Department of Chemistry The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionInstitute for Advanced StudyDepartment of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyHong KongChina Australia-China Joint Centre for Personal Health Technologies Medical Device Research InstituteCollege of Science and EngineeringFlinders UniversitySouth Australia 5042Australia Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates South China University of TechnologyGuangzhou 510640China

Photodynamic therapy(PDT)has emerged as an effective treatment method for its few adverse effects,little invasiveness,short treatment time and low cost[1,2].The rapid development of PDT has dramatically boosted its mu... 详细信息

关键词： treatment boosting immunotherapy

AIEgen-enhanced protein imaging:Probe design and sensing mechanisms

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Aggregate

Aggregate 2021年第3期2卷 77-98页

作者： Sanaz Naghibi Tong Chen Amin Jamshidi Ghahfarokhi youhong tang Medical Devices Research Institute and Institute for NanoScale Science and Technology College of Science and EngineeringFlinders UniversitySouth AustraliaAustralia Davies Livestock Research Centre School of Animal and Veterinary SciencesThe University of AdelaideSouth AustraliaAustralia

Proteins are the building blocks of life,regarded as one of the most complex and crucial biomacromolecules in biological systems,and playing a significant role in executing genes and transferring genetic *** to recent research,due to the structural intricacy of proteins and their sensitivity to physical and chemical degradation processes,they could be utilized as biomarkers or therapeutic agents in the diagnosis,treatment,or even prevention of different ***,modern pathways have been developed for understanding the function of proteins,resulting in intriguing approaches in the field of protein-related *** diagnostic strategies to deal with such diseases,including protein analysis,protein quantification,and protein imaging,were argued in ***,the aggregationinduced emission(AIE)concept and its potential applications for real-time imaging make AIE luminogens(AIEgen)attractive for protein *** general,AIEgens refer to those luminogenic chemicals that are nonluminescent in solution,but luminescent in either the aggregated or solid *** review is focused on the emergence of AIE materials in protein tracking,detecting,and imaging for medical applications.

关键词： AIE materials confocal microscopy fluorescence imaging real-time protein imaging theranostic imaging

Organoclay/thermotropic liquid crystalline polymer *** VI:Effects of intercalated organoclay on nanocomposite morphology,thermal and rheological properties

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International Journal of Smart and Nano Materials 2010年第3期1卷 173-186页

作者： youhong tang Ping Gao Lin Ye Chengbi Zhao Wei Lin Department of Chemical and Biomolecular Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Centre for Advanced Materials Technology School of AerospaceMechanical and Mechatronic EngineeringThe University of SydneySydneyNSW 2006Australia Centre for Advanced Marine Materials School of Civil Engineering and TransportationSouth China University of TechnologyGuangzhou 510641China

A study of a typical intercalated structure of a thermotropic liquid crystalline polymer(TLCP)with organoclay was performed to elucidate the influence of intercalated organoclay on the TLCP molecules,especially on their liquid crystallinity,thermal and rheological *** intercalated structures were confirmed in TLCP and organoclay formed molecular interactions with TLCP molecules in the *** intercalated structures caused the glass transition temperature of the nanocomposite to become invisible in thermal measurement and also caused loss of liquid *** TLCP molecules inside the organoclay galleries showed higher thermal stability and transition temperatures,but the orderly structure of the TLCP molecules outside the galleries was destroyed by the organoclay,causing the TLCP to display lower thermal stability and transition temperatures than pristine *** 185℃,where TLCP is in the nematic phase,the nanocomposite had three orders of magnitude higher viscosity in the linear viscoelastic region than that of TLCP,with chain mobility and relaxation time slowed due to the intercalated effects in the *** shear altered the domain sizes and oriented the highly anisotropic organoclay layers or tactoids along the shear direction.

关键词： intercalation clay liquid crystalline polymer(LCP) rheology

Multiplexed imaging detection of live cell intracellular changes in early apoptosis with aggregation-induced emission fluorogens

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Science China Chemistry 2018年第8期61卷 892-897页

作者： Yabin Zhou Haixiang Liu Na Zhao Zhiming Wang Michael Z.Michael Ni Xie Ben Zhong tang youhong tang Faculty of Biological Engineering Sichuan University of Science and Engineering Department of Chemistry the Hong Kong University of Science and Technology Centre for Nano Scale Science and Technology College of Science and Engineering Flinders University Department of Gastroenterology and Hepatology Flinders Centre for Innovation in Cancer Flinders University AIEgen Biotech Co. Limited

Apoptosis is an important process for maintaining tissue homeostasis and eliminating abnormal cells in multicellular *** in apoptosis often leads to severe diseases such as cancers. Better understanding of its mechanisms and processes is therefore important. Accompanying molecular biology events of apoptosis is a series of cellular morphology changes: nucleus condensation, cell shrinkage and rounding, cell surface blebbing, dynamic blebbing, apoptotic membrane protrusions and nucleus fragmentations and finally, the formation and release of apoptotic bodies. It is difficult to detect cellular changes in the early phase of apoptosis due to the subtle changes at this phase. In the current study, we induced apoptosis in He La cells with H2 O2 and used nuclear dye Hoechst 33258, mitochondria, lysosome and cytoplasmic protein specific aggregation-induced emission fluorogens(AIEgens), TPE-Ph-In, 2 M-DABS and BSPOTPE to successfully perform live cell multiplexed imaging to investigate early apoptosis cellular events. We showed the gradual dissipation of mitochondria membrane potential until it is nondetectable by TPE-Ph-In. Increased mitophagy detected by TPE-Ph-In and 2 M-DABS, condensed nucleus detected by Hoechst33258, increased permeability and/or reduced integrity of nuclear membrane, and increased intracellular vesicles detected by2 M-DABS are some of the early events of apoptosis.

关键词： apoptosis multiplexed imaging He La aggregation-induced emission fluorogens(AIEgens)

Interactions between stearic acid and calcite surfaces:Experimental and computer simulation studies

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Biosurface and Biotribology 2021年第3期7卷 126-132页

作者： Lihua Zhang Laibao Liu Xiaopeng Li youhong tang Chuanbei Liu Hong-Ping Zhang Engineering Research Center of Biomass Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest University of Science and TechnologyMianyangSichuanChina Institute for NanoScale Science and Technology College of Science and EngineeringFlinders UniversityAdelaideSouth AustraliaAustralia

Calcite surface was modified with stearic acids,and the interaction between them was investigated both experimentally and ***-acid-modified calcite powders were investigated with Fourier transform infrared spectroscopy,X-ray diffraction,zeta potential analyser and contact angle ***,the density functional theory calculations were performed to explore the interaction at the atomic *** experi-mental results and simulation indicated that stearic acids interact with calcite surface via chelation between the double-bond O atom of-COOH and the Ca atom on calcite *** terminal methyl group of the stearic acids decreases the hydrophilicity of the calcite surface,and the interaction between different crystal faces of calcite is different.

关键词： surface. measurement. stearic