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Comparative transcriptome analysis of global effect of ddh and lysE deletion on 4-hydroxyisoleucine production in Corynebacterium glutamicum

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Systems Microbiology and Biomanufacturing 2022年第3期2卷 542-554页

作者： Rui Chen youhe xiang Xinping Yu Meiling Zhang Feng Shi State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxi 214122China Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China International Joint Laboratory On Food Safety Jiangnan UniversityWuxi 214122China

4-hydroxyisoleucine(4-HIL)is a potential drug for diabetes and weight control.4-HIL was produced by expressing ido gene in L-isoleucine(Ile)-producing Corynebacterium *** L-lysine(Lys)was also accumulated as the main by-product in this recombinant strain *** attenuate Lys synthesis,two genes in Lys synthetic pathway,i.e.,ddh encoding the diaminopimelic acid dehydrogenase and lysE encoding the specific Lys exporter were deleted in ***,the deletion of ddh increased 4-HIL titer by 28.1%,but did not decrease Lys content;while the deletion of lysE significantly reduced Lys content by 66.7%,but 4-HIL titer also decreased by 19.3%.Therefore,we carried out transcriptome analysis to reveal the global variation in these *** of ddh and lysE(especially lysE)enhanced the transcription of key enzymes in succinylase branch of Lys synthesis pathway(DapD and DapC)and several enzymes involved in succinyl-CoA accessibility(SucC,SucD and OdhI),suggesting the compensatory synthesis of Lys via succinylase *** addition,the transcription of ilvBN in Ile synthesis pathway was improved,while the transcription of some genes in the 2-methylcitrate cycle and inositol metabolism pathway was weakened in these *** deletion of ddh enhanced the transcription of aceA,ppc and pck,thus promoting oxaloacetate supply and 4-HIL *** of lysE affected the transcription of some stress-related genes and transporter genes,suggesting that this mutant would be under stress,thus attenuating its 4-HIL *** findings will be helpful for systematic microbiology and bio-manufacturing of ***.

关键词： 4-hydroxyisoleucine Corynebacterium glutamicum Transcriptome L-lysine

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Critical current degradation in an epoxy-impregnated rare-earth Ba_(2)Cu_(3)O_(7-x)coated conductor caused by damage during a quench

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Applied Mathematics and Mechanics(English Edition) 2024年第9期45卷 1557-1572页

作者： Donghui LIU Huadong YONG youhe ZHOU Institute of Superconductor Mechanics Lanzhou UniversityLanzhou 730000China Department of Mechanics and Engineering Sciences College of Civil Engineering and MechanicsLanzhou UniversityLanzhou 730000China Key Laboratory of Mechanics on Disaster and Environment in Western China Ministry of Education of ChinaLanzhou UniversityLanzhou 730000China

High-temperature superconducting(HTS)rare-earth Ba_(2)Cu_(3)O_(7-x)(REBCO)coated conductors(CCs)have significant potential in high-current and high-field ***,owing to the weak interface strength of the laminated composite REBCO CCs,the damage induced by the thermal mismatch stress under a combination of epoxy impregnation,cooling,and quenching can cause premature degradation of the critical *** this study,a three-dimensional(3D)electromagnetic-thermal-mechanical model based on the H-formulation and cohesive zone model(CZM)is developed to study the critical current degradation characteristics in an epoxy-impregnated REBCO CC caused by the damage during a *** temperature variation,critical current degradation of the REBCO CC,and its degradation onset temperature calculated by the numerical model are in agreement with the experimental data taken from the *** delamination of the REBCO CC predicted by the numerical model is consistent with the experimental *** numerical results also indicate that the shear stress is the main contributor to the damage propagation inside the REBCO *** premature degradation of the critical current during a quench is closely related to the interface shear strength inside the REBCO ***,the effects of the coefficient of thermal expansion(CTE)of the epoxy resin,thickness of the substrate,and substrate material on the critical current degradation characteristics of the epoxy-impregnated REBCO CC during a quench are also *** results help us understand the relationship between the current-carrying degradation and damage in the HTS applications.

关键词： epoxy-impregnated rare-earth Ba_(2)Cu_(3)O_(7-x)(REBCO)coated conductor(CC) quench damage critical current degradation shear stress

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Wavelet Multi-Resolution Interpolation Galerkin Method for Linear Singularly Perturbed Boundary Value Problems

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Computer Modeling in Engineering & Sciences 2024年第4期139卷 297-318页

作者： Jiaqun Wang Guanxu Pan youhe Zhou Xiaojing Liu School of Management Science and Engineering Anhui University of Finance and EconomicsBengbu233030China Key Laboratory of Mechanics on Disaster and Environment in Western China The Ministry of EducationCollege of Civil Engineering and MechanicsLanzhou UniversityLanzhou730000China

In this study,a wavelet multi-resolution interpolation Galerkin method(WMIGM)is proposed to solve linear singularly perturbed boundary value *** conventional wavelet schemes,the proposed algorithm can be readily extended to special node generation techniques,such as the Shishkin *** a wavelet method allows a high degree of local refinement of the nodal distribution to efficiently capture localized steep *** the shape functions possess the Kronecker delta property,making the imposition of boundary conditions as easy as that in the finite element *** numerical examples are studied to demonstrate the validity and accuracy of the proposedwavelet *** results showthat the use ofmodified Shishkin nodes can significantly reduce numerical oscillation near the boundary *** with many other methods,the proposed method possesses satisfactory accuracy and *** theoretical and numerical results demonstrate that the order of theε-uniform convergence of this wavelet method can reach 5.

关键词： Wavelet multi-resolution interpolation Galerkin singularly perturbed boundary value problems mesh-free method Shishkin node boundary layer

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Three-dimensional simulation of the coupled electromagneticmechanical responses in a no-insulation racetrack coil

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Science China Technological Sciences 2024年

作者： LI DongKe CHANG Zhixiang TANG YunKai LIU DongHui YONG HuaDong ZHOU youhe Key Laboratory of Mechanics on Disaster and Environment in Western China Ministry of Education of China Lanzhou University Department of Mechanics and Engineering Sciences College of Civil Engineering and Mechanics Lanzhou University

This study investigates the coupled behavior of the mechanical and electromagnetic responses of a high-temperature superconducting(HTS) no-insulation(NI) racetrack coil. A three-dimensional(3D) equivalent circuit network model and mechanical model with nonlinear contact are incorporated into the coupled model. The stress and separation of adjacent turns under a high magnetic field are analyzed by considering the electromagnetic force and cooling process. The numerical results demonstrate that the straight and curved parts of the racetrack coil exhibit distinct mechanical behaviors. Under a strong external magnetic field, the separation between adjacent turns reduces the charging delay of the coil. The maximum stress occurs in the transition regions between the curved and straight parts. The straight part exhibits a larger rotation angle, while the separation regions between adjacent turns are relatively fewer in those parts. The curved part shows a higher proportion of separation regions in the steady stage. The different separation areas in the straight and curved parts also result in distinct turn-to-turn losses during the charging process. Subsequently, a comparative study is conducted on the influence of residual currents induced by ***, the effects of overband and inner diameter on the separation behavior are investigated.

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浙江省医疗保险保障水平与医疗卫生服务水平的匹配度研究

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医学与社会 2024年第1期37卷 137-144页

作者：孔佑赫叶明华华东师范大学统计学院上海200062

目的:测算与评估浙江省医疗保险保障水平与医疗卫生服务水平的匹配度,为医疗卫生服务水平与医疗保险保障水平的协调发展提供参考。方法:以浙江省11个地级市为研究对象,利用灰色关联度求权重法和基于熵权法的Topsis,测算评估医疗保险保... 详细信息

目的:测算与评估浙江省医疗保险保障水平与医疗卫生服务水平的匹配度,为医疗卫生服务水平与医疗保险保障水平的协调发展提供参考。方法:以浙江省11个地级市为研究对象,利用灰色关联度求权重法和基于熵权法的Topsis,测算评估医疗保险保障水平与医疗卫生服务水平之间的匹配度。结果:从医疗保险保障来看,浙江省医疗保险保障广度、深度、效率数值分别处于0.0485-0.1478区间、0.0640-0.1541区间、0.0531-0.1414区间,区间跨度较大;从医疗卫生服务来看,浙江省医疗卫生服务广度和深度指标区间跨度分别达到0.2526、0.2863。杭州综合得分(0.2731),显著高于其他城市。从总体来看,浙江省各地级市医疗保险保障水平与医疗卫生服务水平的匹配度得分有所差异,区间跨度达0.1542。结论:浙江省医疗保险保障分布不均衡;医疗卫生服务空间差异明显;医疗保险保障水平和医疗卫生服务水平存在不匹配,具体表现为医疗卫生服务滞后影响医疗保险保障效用提升、医疗保险保障不足影响医疗卫生服务供给两方面。建议通过政府支持、医疗卫生服务资源下沉、机构数据互通实现优化改善。

关键词：医疗保险医疗卫生服务匹配度浙江

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MODIFICATION OF ONE-DIMENSION COUPLED HYSTERESIS MODEL FOR GMM WITH THE DOMAIN FLEXING FUNCTION

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Acta Mechanica Solida Sinica 2014年第5期27卷 461-466页

作者： Yongfei Zhang youhe Zhou Key Laboratory of Mechanics on Disaster and Environment in Western China attached to the Ministry of Education of China Lanzhou University Department of Mechanics and Engineering Sciences College of Civil Engineering and Mechanics Lanzhou University

This paper focuses on eliminating the unphysical negative susceptibility which ap- pears when magnetic field is at unsaturated excitation level and reduces from extremity of the hysteresis loop in one-dimension coupled hysteresis model. The domain flexing function c （H） is used to replace the domain flexing constant c in one-dimension coupled hysteresis model. The fea- sibility and rationality of proposed modification are convinced by comparing the magnetization and magnetostriction curves with experimental data and another typical modification results. The effects of pre-stress and temperature on magnetic-elastic-thermal coupling property and hysteresis behavior are investigated.

关键词： coupled hysteresis model domain flexing function negative susceptibility innerloop

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Electric-magnetic-force characteristics of rare earth barium copper oxide superconductor high-field coils based on screening effect and strain sensitivity

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Applied Mathematics and Mechanics(English Edition) 2022年第8期43卷 1249-1268页

作者： Wenhai ZHOU youhe ZHOU Key Laboratory of Mechanics on Environment and Disaster in Western China The Ministry of Education of ChinaCollege of Civil Engineering and MechanicsLanzhou UniversityLanzhou730000China School of Petrochemical Technology Lanzhou University of TechnologyLanzhou730050China

Rare earth barium copper oxide(REBCO)is the most researched and commercialized second-generation high-temperature superconducting *** to the anisotropic structure,strong deformation sensitivity,and central field errors caused by screening current effects,it is still a challenge for commercialization *** this study,the transversely isotropic constitutive relationship is selected as the mechanical model based on the structural characteristics of REBCO tapes,and suitable microelements are selected to equate the elastic constants using their average stress-strain ***,a two-dimensional axisymmetric model for coils wound by single-layer tapes is constructed to analyze the dependence of the electric-magnetic-force distribution in the tape on the ***,the anisotropic approximation of the homogenized bulk method is used to equate large-turn high-field coils,and the electric-magnetic-force distribution characteristics of the coils with/without screening effects and mechanical strain conditions are investigated,*** results reveal that the mechanical strain has a weakening effect on the electromagnetic field distribution of superconducting tapes,but causes a significant enhancement in the force field *** the presence of 0.5% mechanical strain,the maximum weakening of the peak value of the current density and the critical current density inside the high-field coil can reach about 8% and 13%,respectively,with a nearly 5 times increase in the peak *** screening current makes the current field distribution inside the coil improve by about 10 *** screening current induced magnetic field can reach up to 0.8 T,making the relative error of the high-field coil center up to 7.8%.

关键词： screening current strain sensitivity mechanical behavior rare earth barium copper oxide(REBCO)high-field coil electromagnetic field distribution

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A MAGNETO-MECHANICAL FULLY COUPLED MODEL FOR GIANT MAGNETOSTRICTION IN HIGH TEMPERATURE SUPERCONDUCTOR

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Acta Mechanica Solida Sinica 2015年第4期28卷 353-359页

作者： Zhiwen Gao youhe Zhou Key Laboratory of Mechanics on Disaster and Environment in Western China attached to the Ministry of Education of China Department of Mechanics and Engineering Sciences College of Civil Engineering and Mechanics Lanzhou University

This paper presents a fully coupled model to account for the flux pinning induced giant magnetostriction in type-Ⅱ superconductors under alternating magnetic field The superconductor E-J constitutive law is characterized by power law where the critical current density is assumed to depend exponentially on the flux density. The governing equations of the two-field problem （i.e., the interactions of elastic and magnetic effects） are formulated in a two-dimensional model. The magnetostriction curves and magnetization loops are calculated over a wide range of parameters. The effects of applied magnetic field frequency f and amplitude B0 and critical current density on magnetostriction and magnetization are discussed. Results show that the critical current density of high temperature superconductor （HTS） YBCO has a significant effect on the magnetization and magnetostriction. The pinning-induced magnetostriction which has been observed in experiment can be qualitatively simulated by this model.

关键词： high temperature superconductor (HTS) giant magnetostriction magnetization,magneto-mechanical couple alternating magnetic field

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Now is the time to test early urinary biomarkers in large-scale human samples

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Science China(Life Sciences) 2019年第6期62卷 851-853页

作者： youhe Gao Gene Engineering Drug and Biotechnology Beijing Key Laboratory School of Life Sciences Beijing Normal University

Although blood is the most common source for biomarkers,urine has not been completely ignored. There have been some urinary biomarker studies with clinical ***, most-if not all-biomarker researchers lack confidence in urine as a good biomarker source. Many researchers think that good results from urine are a statistical accident. To demonstrate the validity of urinary biomarkers,large-scale studies are needed. Until such convincing evidence exists, what funding agent could support research in this field?

关键词： Although blood biomarkers what funding

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Facts of research on protein-protein interactions

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Science China(Life Sciences) 2017年第3期60卷 313-314页

作者： youhe Gao Gene Engineering and Biotechnology Beijing Key Laboratory Department of Biochemistry and Molecular Biology Beijing Normal University Beijing 100875 China

Dear Editor,Fact one of the current research on protein-protein interactions is that tagging is widely *** is a good way to track or visualize a *** assume that tagging does not change the structure and function of th... 详细信息

关键词：蛋白质相互作用生命科学科学研究

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