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Journal of Genetics and Genomics 2013年第10期40卷 533-542页

作者： Peng-Juan Liang Wen-yuan Han Qi-Hong Huang Yan-Ze Li jin-Feng Ni Qun-Xin She Yu-Long Shen State Key Laboratory of Microbial Technology Shandong University Archaeal Centre Department of BiologyUniversity of CopenhagenOle Maal es Vej 5Copenhagen BiocenterDK-2200 CopenhagenDenmark

RecA family recombinases play essential roles in maintaining genome integrity. A group of RecA-like proteins named RadC are present in all archaea, but their in vivo functions remain unclear. In this study, we performed phylogenetic and genetic analysis of two RadC proteins from Sulfolobus islandicus. RadC is closer to the KaiC lineage of cyanobacteria and proteobacteria than to the lineage of the recombinases （RecA, RadA, and Rad51） and the recombinase paralogs （e.g., RadB, Rad55, and Rad51B）. Using the recently- established S. islandicus genetic system, we constructed deletion and over-expression strains of radC1 and radC2. Deletion of radC1 rendered the cells more sensitive to DNA damaging agents, methyl methanesulfonate （MMS）, hydroxyurea （HU）, and ultraviolet （UV） radiation, than the wild type, and a AradCIAradC2 double deletion strain was more sensitive to cisplatin and MMS than the AradC1 single deletion mutant. In addition, ectopic expression of His-tagged RadC 1 revealed that RadC I was co-purified with a putative structure-specific nuclease and ATPase, which is highly conserved in archaea. Our results indicate that both RadCI and RadC2 are involved in DNA repair. RadCl may play a general or primary role in DNA repair, while RadC2 plays a role in DNA repair in response to specific DNA damages.

关键词： RecA-like protein RadA RadC Sulfolobus DNA repair

Isolation of Human Antibodies Against Hepatitis E Virus From Phage Display Library by Immobilized Metal Affinity Chromatography

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Biomedical and Environmental Sciences 2007年第6期20卷 488-494页

作者： YING-WEI CHEN WEN-XIN LUO MING-QIAO WANG jin WANG LI-FENG LI QUAN yuan JUN ZHANG NING-SHAO XIA National Institute of Diagnostic and Vaccine Development for infectious diseases The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen 361005 Fujian China

Objective To isolate human antibodies against hepatitis E virus from phage display library by a new method of panning phage antibody library based on immobilized metal affinity chromatography （1MAC）. Methods Phage antibody library was allowed to mix with hex-His tagged expressed HEV specific antigen, NE2, in solution for adequate binding before affinity resin for hex-His was added. The non-specific phage antibodies were removed by extensive washing and the specific bound phage antibodies could then be eluted to infect TG1 or repeat the binding process for subsequent rounds of purification. The specificity of the selected human antibodies were tested by antigen competitive ELISA, human sera blocking ELISA, scFv expression, and sequence analysis. Results His-NE2 specific recombinant phages were successfully enriched after panning procedure. Two individual phage clones, 126 and 138, showed 50% inhibition in NE2 antigen competition ELISA and obvious blocking effect by HEV positive serum in blocking ELISA. Soluble scFv of 126, 138 bound to NE2 specifically. Conclusion Two specific human phage antibodies against hepatitis E virus （HEV） from phage display library were isolated by immobilized metal affinity chromatography. The immobilized metal affinity chromatography applied to phage antibody selection was a helpful supplement to the selection in solution.

关键词： HEV antibodies Phage antibody library Affinity chromatography Panning

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8×8重排无阻塞型SOI热光波导开关阵列

激光与红外 2009年第1期39卷 46-49页

作者：陈媛媛余金中北京工商大学信息工程学院北京100037 中国科学院半导体研究所集成光电子国家重点实验室北京100083

设计并制作了一种重排无阻塞型的8×8SOI热光波导开关阵列。开关单元采用了MMI—MZI结构的2×2光开关。整个器件的开关时间约为2μs。器件中开关单元功耗小于240mw。消光比在17-22dB范围内变化。功耗和开关速度都明显优于SiO2基和聚合... 详细信息

设计并制作了一种重排无阻塞型的8×8SOI热光波导开关阵列。开关单元采用了MMI—MZI结构的2×2光开关。整个器件的开关时间约为2μs。器件中开关单元功耗小于240mw。消光比在17-22dB范围内变化。功耗和开关速度都明显优于SiO2基和聚合物基的开关阵列。

关键词：开关阵列热光 SOI

4f^(N-1)5d组态自旋允许与禁戒跃迁能级的研究

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无机化学学报 2006年第3期22卷 399-402页

作者：师进生曲郸张思远莱阳农学院中国科学院长春应用化学研究所

通过稀土光谱理论,计算了三价稀土离子的4fN-15d组态能级的f与d电子间的库仑相互作用,结果表明对于重稀土元素,其自旋允许跃迁能级位置高于自旋禁戒跃迁能级位置,并且两种能级间的能级差随f电子数的增加而依次减小。对于轻稀土元素,则... 详细信息

通过稀土光谱理论,计算了三价稀土离子的4fN-15d组态能级的f与d电子间的库仑相互作用,结果表明对于重稀土元素,其自旋允许跃迁能级位置高于自旋禁戒跃迁能级位置,并且两种能级间的能级差随f电子数的增加而依次减小。对于轻稀土元素,则自旋禁戒跃迁能级位置高于自旋允许跃迁能级位置。结果很好地解释了自旋禁戒跃迁能级的光谱现象。

关键词：稀土离子自旋禁戒跃迁带 5d能级库仑作用

Indole Alkaloids from Marine Fungi Collected from the South China Sea

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Indole Alkaloids from Marine Fungi Collected from the South ...

Analysis of ST-elevated acute myocardial infarction in the subacute phase after stent implantation

中国化学会第十二届全国天然有机化学学术会议

作者： Mei-Xiang yuan Yan-Qin Ran Yi Qiu Hou-jin Li School of Chemistry Sun Yat-sen University School of Traditional Chinese Medicine Guangdong Pharmaceutical University

＜正＞As we all known,alkaloids attracted the pharmacologists’ and chemists’ interests for their diverse skeletons and broad spectra of bioactivities,such as antihistaminic,antifungal,antimicrobial,antioxidant,plant growth regulator,anti-HIV,anticonvulsant,anti-inflammatory and analgesic activities[1].In 1866,Adolf von Baeyer firstly got indole from oxindole using zinc *** alkaloids are widely distributed in the natural environment,and up to now more than 1000 indole alkaloids have been isolated from microorganisms.

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Analysis of ST-elevated acute myocardial infarction in the s...

AISI9310钢薄辐板齿轮热处理变形与残余应力数值模拟及验证

第十三次全国心血管病学术会议

作者： SONG Guang-yuan,YANG Yue-jin,XU Bo,GAO Run-lin,LI Jian-jun, QIAO Shu-bin,yuan jin-qing,you Shi-jie,PEI Han-jun,WU Yong-jian Cardiovascular Institute and Fu Wai Hospital,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100037,China

Objective ST-elevated acute myocardial infarction(STEAMI) happens in the first month post percutaneous coronary intervention(PCI) is almost related to acute thrombosis or subacute thrombosis *** article is to investigate the possible causes of AMI one month *** Patients who had a history of successful PCI,and also received coronary angiography or re-PCl due to STEAMI this time were included in this *** was recorded in detail that the AMI-related

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材料热处理学报 2022年第3期43卷 151-159页

作者：李承然唐进元中南大学机电工程学院湖南长沙410083 高性能复杂制造国家重点实验室湖南长沙410083

齿轮热处理时伴随着剧烈的温度变化和复杂的组织演变,此过程中通常会产生变形和应力。基于温度场-渗碳场-组织场-应力应变场耦合模型,采用有限元方法对AISI9310钢薄辐板齿轮的热处理过程进行数值模拟,研究了淬火时轮齿组织与应力演变规... 详细信息

齿轮热处理时伴随着剧烈的温度变化和复杂的组织演变,此过程中通常会产生变形和应力。基于温度场-渗碳场-组织场-应力应变场耦合模型,采用有限元方法对AISI9310钢薄辐板齿轮的热处理过程进行数值模拟,研究了淬火时轮齿组织与应力演变规律及冷处理和回火过程对轮齿组织含量和应力大小的影响,得到了齿轮热处理后的最终的残余应力和变形。为验证模拟计算结果准确性,采用三坐标测量机和X射线残余应力衍射仪对热处理后齿轮尺寸和残余应力进行了测量。结果表明:淬火时轮齿表面马氏体相变时间远远滞后于心部,淬火结束后表面呈现压应力,心部为拉应力;冷处理时渗碳表面的残留奥氏体发生了马氏体转变,表面压应力增大,而回火则使应力有所减小,最终应力模拟计算结果与实测值相对误差最大为11.9%;论文中的数值模拟方法具有较好的精度,可以应用于金属齿轮的热处理工艺仿真计算,具有较大的参考价值。

关键词：薄辐板齿轮热处理有限元残余应力变形

New open cluster candidates discovered in the XSTPS-GAC survey

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Research in Astronomy and Astrophysics 2018年第3期18卷 77-90页

作者： jin-Cheng Guo Hua-Wei Zhang Hui-Hua Zhang Xiao-Wei Liu Hai-Bo yuan Yang Huang Song Wang Li Chen Hai-Bin Zhao Ji-Feng Liu Bing-Qiu Chen Mao-Sheng Xiang Zhi-Jia Tian Zhi-Ying Huo Chun Wang Department of Astronomy Peking University Kavli Institute for Astronomy and Astrophysics Peking University Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of Sciences South-Western Institute for Astronomy Research Yunnan University Department of Astronomy Beijing Normal University Shanghai Astronomical Observatory Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

The Xuyi Schmidt Telescope Photometric Survey of the Galactic Anti-center（XSTPS-GAC）is a photometric sky survey that covers nearly 6000 deg^2 towards the Galactic Anti-center（GAC） in the g,r,i bands. Half of its survey field is located on the Galactic Anti-center disk,which makes XSTPSGAC highly suitable to search for new open clusters in the GAC region. In this paper,we report new open cluster candidates discovered in this survey,as well as properties of these open cluster candidates,such as age,distance and reddening,derived by isochrone fitting in the color-magnitude diagram（CMD）.These open cluster candidates are stellar density peaks detected in the star density maps by applying the method from Koposov et al. Each candidate is inspected in terms of its true color image composed from three XSTPS-GAC band images. Then its CMD is checked,in order to identify whether the central region stars have a clear isochrone-like trend differing from background stars. The parameters derived from isochrone fitting for these candidates are mainly based on three band photometry of ***,when these new candidates are able to be seen clearly in 2 MASS data,their parameters are also derived based on the 2 MASS（J-H,J） CMD. There are a total of 320 known open clusters rediscovered and 24 new open cluster candidates discovered in this work. Furthermore,the parameters of these new candidates,as well as another 11 previously known open clusters,are properly determined for the first time.

关键词： open clusters and associations general - Galaxy structure - methods data analysis

酸胁迫作用下悬浮培养红豆杉细胞的磁场生物学效应

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过程工程学报 2005年第3期5卷 345-348页

作者：范寰商桂敏元英进天津大学化工学院制药工程系天津300072

研究了直流稳恒磁场对酸胁迫作用下悬浮培养南方红豆杉细胞生长和代谢的影响.结果表明,磁场提高了红豆杉细胞的抗酸胁迫能力,细胞活力上升,生物量和蛋白含量明显升高,胞外多酚氧化酶(PPO)活力升高,酚类物质积累受到抑制.在一定浓度的盐... 详细信息

研究了直流稳恒磁场对酸胁迫作用下悬浮培养南方红豆杉细胞生长和代谢的影响.结果表明,磁场提高了红豆杉细胞的抗酸胁迫能力,细胞活力上升,生物量和蛋白含量明显升高,胞外多酚氧化酶(PPO)活力升高,酚类物质积累受到抑制.在一定浓度的盐酸作用下细胞受到的酸胁迫越大,磁场的生物学效应越明显.胞外pH值和电导率的变化说明磁场提高细胞抗酸胁迫的能力与其加速了物质的跨膜转运有关.

关键词：直流稳恒磁场南方红豆杉细胞酸胁迫跨膜运输

Coexistent Wegener's granulomatosis with pulmonary cryptococcosis and maxillare aspergillosis- a case report

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Coexistent Wegener's granulomatosis with pulmonary cryptococ...