检索结果-南通市图书馆

Biallelic mutations in spermatogenesis and centriole-associated 1 like(SPATC1L)cause acephalic spermatozoa syndrome and male infertility

引用

Asian Journal of Andrology 2022年第1期24卷 67-72页

作者： you-zhu li Na li Wen-Sheng liu Yan-Wei Sha Rong-Feng Wu Ya-ling Tang Xing-Shen zhu Xiao-li Wei Xiao-Ya Zhang Yi-Feng Wang Zhong-Xian Lu Fu-Xing Zhang Reproductive Medicine Center The First Affiliated Hospital of Xiamen UniversityXiamen 361003China Intensive Care Unit Fujian Medical University Xiamen Humanity HospitalXiamen 361009China Obstetrics and Gynecology Center Department of Obstetrics and GynecologyZhujiang HospitalSouthern Medical UniversityGuangzhou 510280China School of Pharmaceutical Sciences State Key Laboratory of Cellular Stress BiologyXiamen UniversityXiamen 361102China Department of Andrology United Diagnostic and Research Center for Clinical GeneticsWomen and Children’s Hospital and School of MedicineXiamen UniversityXiamen 361005China Department of Obstetrics and Gynecology First Affiliated Hospital of Xiamen UniversityXiamen 361003China Department of General Surgery First Affiliated Hospital of Xiamen UniversityXiamen 361003China

Acephalic spermatozoa syndrome is a rare type of teratozoospermia that severely impairs the reproductive ability of male patients,and genetic defects have been recognized as the main cause of acephalic spermatozoa syndrome.Spermatogenesis and centrioleassociated 1 like(SPATC1L)is indispensable for maintaining the integrity of sperm head-to-tail connections in mice,but its roles in human sperm and early embryonic development remain largely unknown.Herein,we conducted whole-exome sequencing(WES)of 22 infertile men with acephalic spermatozoa syndrome.An in silico analysis of the candidate variants was conducted,and WES data analysis was performed using another cohort consisting of 34 patients with acephalic spermatozoa syndrome and 25 control subjects with proven fertility.We identified biallelic mutations in SPATC1L(c.910C>T:p.Arg304Cys and c.994G>T:p.Glu332X)from a patient whose sperm displayed complete acephalia.Both SPATC1L variants are rare and deleterious.SPATC1L is mainly expressed at the head–tail junction of elongating spermatids.Plasmids containing pathogenic variants decreased the level of SPATC1L in vitro.Moreover,none of the patient’s four attempts at intracytoplasmic sperm injection(ICSI)resulted in a transplantable embryo,which suggests that SPATC1L defects might affect early embryonic development.In conclusion,this study provides the first identification of SPATC1L as a novel gene for human acephalic spermatozoa syndrome.Furthermore,WES might be applied for patients with acephalic spermatozoa syndrome who exhibit reiterative ICSI failures.

关键词： acephalic spermatozoa syndrome biallelic mutations spermatogenesis and centriole-associated 1 like whole-exome sequencing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Establishment of hepatocarcinoma cell line transfected by the B7 gene and its biocharacteristics

引用

Hepatobiliary & Pancreatic Diseases International 2003年第2期2卷 594-596页

作者： you-zhu li Quan Wang Yan-Fang Jiang the First Clinical Hospital, Jilin University, Changchun 130021, China First Clinical Hospital Jilin University Changchun 130021 China. leeyz@***

OBJECTIVE: To study the function of costimulation sign in tumor immunology and construct the new cell line B7^+ Smmc7721. METHODS: The B7 gene was transfected into the hepatocarcinoma cell Smmc7721 by liposma. Polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-PCR) were applied to test the result. MTT colorimetric assay was used to value the killing effect of lymphokine activated killer cells (LAK) activated by IL-2 on the transfected cell line and the original line. RESULT: B7^+ Smmc7721 was improved to be steadily expressed B7 molecule and LAK cells could more effectively act on the B7^+ Smmc7721 cells. CONCLUSION: The B7 gene can be transfected to hepatocarcinoma cells and can be expressed steadily in vitro, thus increasing the efficiency of LAK cells activated by IL-2 on them.

关键词： costimulation molecule hepatocarcinoma gene transfection gene therapy immune therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Vitrification versus Slow Freezing of Human Oocytes: Effects on Ultrastructure and Developmental Potential

引用

Reproductive and Developmental Medicine 2018年第3期2卷 129-136页

作者： you-zhu li Na li Xiao-Hong Yan Wei-Dong Zhou Yu-Lai Zhou Qiong-Hua Chen Rong-Feng Wu Center for Reproductive Medicine The First Affiliated Hospital of Xiamen UniversityXiamen 361003China Intensive Care Unit Xiamen Hongai HospitalXiamen 361003China College of Pharmacy Jilin UniversityChangchun 130021China

Objective:This study compared spindles,cytoskeleton,and developmental potential between vitrified and slow-frozen oocytes using PolScope and electron microscopy.Methods:Oocytes were randomly divided into control,slow freeze-thaw,and vitrification freeze-thaw groups(0,1,and 3 h).PolScope was used to observe spindles,angle of spindles to the first polar bodies,surface areas of oocytes,and lining and outer ret of zona pellucida.Surfaces and ultrastructure of oocytes were observed by scanning electron microscopy and transmission electron microscopy.These measures were used to characterize the impacts of two freezing methods on the developmental capacity of human oocytes.Results:The visible frequency of spindle formation was 92.4%,56.4%,11.2%,24.8%,and 61.1%in control group,slow freeze-thaw group,and the three vitrification freeze-thaw groups(0,1,and 3 h),respectively.Compared to oocytes in the slow freeze group,the angle of the spindle to the first polar body in oocytes in the 3-h vitrification freeze-thaw group was less(37.3°vs.68°,P=0.023).No significant differences were observed between the surface area of oocytes,or lining and outer ret of oocyte zona pellucida between freeze-thaw in these same two groups.Microvilli appeared normal.However,protrusions on oocyte surfaces were increased,and microvilli were laid down on the membrane surface in the 3-h vitrification freeze-thaw group in comparison to the slow-freeze group.Similar comparisons showed better recovery of perivitelline space and mitochondria between the 3-h vitrified and slow-frozen groups.Bipronuclear(2PN)fertilization rate observed in the slow-freeze group(65.7%)was lower than the rate seen in controls(79.2%,P=0.041).No significant differences were observed in 2PN fertilization,cleavage,and blastocyst formation rates between the 3-h vitrification freeze-thaw and control groups.Conclusions:Results suggest that vitrification freeze-thaw for oocyte cryopreservation was a better choice than slow freeze-thaw.

关键词： Human Oocyte Meiotic Spindle Slow Freezing Vitrification Zona Pellucida

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Vitrification on the Imprinted Gene Snrpn in Neonatal Placental Tissue

引用

Reproductive and Developmental Medicine 2020年第1期4卷 25-31页

作者： Jian-Feng Yao Yan-Fang Huang Rong-Fu Huang Su-Xia lin Cai-Qiong Guo Cheng-Zhou Hua Pei-Ya Wu Ji-Feng Hu you-zhu li Center for Reproductive Medicine Quanzhou Maternity and Child Healthcare HospitalQuanzhou 362000China Health Screening Center The First Affiliated HospitalFujian Medical UniversityFuzhou 350004China Clinical Testing Center The Second Affiliated HospitalFujian Medical UniversityQuanzhou 362000China Department of Obstetrics and Gynecology The First Affiliated HospitalFujian Medical UniversityFuzhou 350004China Center for Reproductive Medicine The First Affiliated Hospital of Xiamen UniversityXiamen 361003China

Objective:To investigate the effects of vitrification on the expression of the imprinted gene Snrpn in neonatal placental tissue.Methods:Neonatal placental tissue was collected from women with natural pregnancy(control group)and from women in assisted reproductive technology(ART)pregnancy group,following fresh and vitrified embryo transfer(fresh group and vitrified group,respectively).Snrpn mRNA expression and SNRPN protein levels in placental tissue from these three groups were assessed by real-time reverse transcription polymerase chain reaction and Western blot,respectively.DNA methylation in the Snrpn promoter region was analyzed by bisulfite-pyrosequencing.Results:The expression of Snrpn mRNA and SNRPN protein was found to be higher in placental tissue from the fresh and vitrified ART groups,compared to the control group.There was no significant difference in SNRPN gene or protein expression between the fresh and vitrified groups.DNA methylation at the Snrpn promoter region was not significantly different between these three groups.Conclusions:Human ART may alter the transcriptional expression and protein levels of the imprinted gene Snrpn.However,compared to other ART methods,vitrification may not aggravate or reduce this effect.Moreover,the altered expression of Snrpn is likely not directly related to DNA methylation of the Snrpn promoter region.

关键词： Assisted Reproductive Technology Polymerase Chain Reaction Snrpn Vitrification Western Blot

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel matrix topology-based analysis method of thermal circuits for reliability optimization of HSER

引用

Chinese Journal of Aeronautics 2024年第4期37卷 479-492页

作者： Huimin liANG Bo li Jiaxin you Shan JIANG Mengtong zhu Aobo WANG Department of Electrical Engineering Harbin Institute of TechnologyHarbin 150001China Nanjing Research Institute of Electronics Technology Nanjing 210008China

Hermetically Sealed Electromagnetic Relay(HSER), used in aviation and aerospace,demands high reliability due to its critical applications. Given its complex operating conditions, efficient thermal analysis is essential for optimizing reliability. The commonly used Finite Element Method(FEM) is often time-consuming and may not be efficient or adaptable for complex multi-dimensional system calculations and design processes. This paper introduces an analysis method for thermal networks based on matrix perspective technology, encompassing matrix transformation, backpropagation of the heat path model, temperature rise calculation, solution comparison, and product implementation. Using the similarity theory of heat circuits, a basic thermal unit is established. Based on the fundamental connection between key components, a thermal network for a typical HSER is designed. An experimental system is set up, and the thermal network model's accuracy is confirmed using test data. Employing the topology analysis method, the topology of the thermal network is analyzed under both coil-energized and de-energized states. Potential thermal paths are identified, leading to optimized solutions for the HSER. Utilizing these solutions, the thermal path matrix topology model is backpropagated to the thermal path for temperature rise calculations. When compared to prototype HSER test data, the efficiency and accuracy of this matrix topology-based analysis method are confirmed.

关键词： Thermal networks HSER(Hermetically Sealed Electromagnetic Relay) Heat-circuit topology model Heat-circuit matrix Optimization design

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Leaching kinetics of fluorine during the aluminum removal from spent li-ion battery cathode materials

引用

Journal of Environmental Sciences 2024年第4期138卷 312-325页

作者： Shengjie li Jianxin zhu Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China

The high content of aluminum(Al)impurity in the recycled cathode powder seriously affects the extraction efficiency of Nickel,Cobalt,Manganese,and lithium resources and the actual commercial value of recycled materials,so Al removal is crucially important to conform to the industrial standard of spent li-ion battery cathode materials.In this work,we systematically investigated the leaching process and optimum conditions associated with Al removal from the cathode powder materials collected in a wet cathode-powder peeling and recycling production line of spent li-ion batteries(liBs).Moreover,we specifically studied the leaching of fluorine(F)synergistically happened along with the removal process of Al,which was not concerned about in other studies,but one of the key factors affecting pollution prevention in the recovery process.The mechanism of the whole process including the leaching of Al and F from the cathode powder was indicated by using NMR,FTIR,and XPS,and a defluoridation process was preliminarily investigated in this study.The leaching kinetics of Al could be successfully described by the shrinking core model,controlled by the diffusion process and the activation energy was 11.14 kJ/mol.While,the leaching of F was attributed to the dissolution of liPF6and decomposition of PVDF,and the kinetics associated was described by Avrami model.The interaction of Al and F is advantageous to realize the defluoridation to some degree.It is expected that our investigation will provide theoretical support for the large-scale recycling of spent liBs.

关键词： Al removal F leaching Cathode powder Heating alkali leaching Leaching kinetics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Large-inductance superconducting microstrip photon detector enabling 10 photon-number resolution

引用

Advanced Photonics 2024年第1期6卷 96-105页

作者： ling-Dong Kong Tian-zhu Zhang Xiao-Yu liu Hao li Zhen Wang Xiao-Ming Xie li-Xing you Chinese Academy of Sciences(CAS) Shanghai Institute of Microsystem and Information TechnologyNational Key Laboratory of Materials for Integrated CircuitsShanghaiChina CAS Center for Excellence in Superconducting Electronics ShanghaiChina University of Chinese Academy of Sciences Center of Materials Science and Optoelectronics EngineeringBeijingChina

Efficient and precise photon-number-resolving detectors are essential for optical quantum information science.Despite this,very few detectors have been able to distinguish photon numbers with both high fidelity and a large dynamic range,all while maintaining high speed and high timing precision.Superconducting nanostrip-based detectors excel at counting single photons efficiently and rapidly,but face challenges in balancing dynamic range and fidelity.Here,we have pioneered the demonstration of 10 true photon-number resolution using a superconducting microstrip detector,with readout fidelity reaching an impressive 98%and 90%for 4-photon and 6-photon events,respectively.Furthermore,our proposed dual-channel timing setup drastically reduces the amount of data acquisition by 3 orders of magnitude,allowing for real-time photon-number readout.We then demonstrate the utility of our scheme by implementing a quantum random-number generator based on sampling the parity of a coherent state,which guarantees inherent unbiasedness,robustness against experimental imperfections and environmental noise,as well as invulnerability to eavesdropping.Our solution boasts high fidelity,a large dynamic range,and real-time characterization for photon-number resolution and simplicity with respect to device structure,fabrication,and readout,which may provide a promising avenue towards optical quantum information science.

关键词： superconducting microstrips single-photon detector photon-number resolution quantum random number

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neuroprotective and anti-inflammatory effects of eicosane on glutamate and NMDA-induced retinal ganglion cell injury

引用

International Journal of Ophthalmology(English edition) 2024年第4期17卷 638-645页

作者： Zhen-Kai Wu Huan-Yu li you-lin zhu Meng-Qin Xiong Jing-Xiang Zhong Department of Ophthalmology the First Affiliated Hospital of Jinan UniversityGuangzhou 510632Guangdong ProvinceChina Changde Hospital Xiangya School of MedicineCentral South University(the First People’s Hospital of Changde City)Changde 415000Hunan ProvinceChina

AIM:To investigate the protective effects,antioxidant potential,and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-Daspartate(NMDA)-induced retinal ganglion cell(RGC)injury in a mouse model of glaucoma.METHODS:The protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide staining.Intracellular reactive oxygen species(ROS)production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow cytometry.The protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of retina.The effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatographymass spectroscopy(LC-MS)and untargeted metabolomics analyses.RESULTS:Eicosane treatment significantly attenuated glutamate-induced damage to R28 cells in vitro.Eicosane also protected RGCs against NMDA-induced injury in a mouse glaucoma model.Untargeted metabolomics analyses showed that eicosane increased multiple metabolites,including L-arginine and L-carnitine,in the retina.CONCLUSION:Eicosane has protective effects,antioxidant potential,and anti-inflammatory properties in an in vitro model of glutamate-induced cell damage and in an in vivo model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism.

关键词： glaucoma metabolites eicosane

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Wood of trees:Cellular structure,molecular formation,and genetic engineering

引用

Journal of Integrative Plant Biology 2024年第3期66卷 443-467页

作者： Yingying zhu Laigeng li State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems and College of Ecology Lanzhou UniversityLanzhou 730000China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China

Wood is an invaluable asset to human society due to its renewable nature,making it suitable for both sustainable energy production and material manufacturing.Additionally,wood derived from forest trees plays a crucial role in sequestering a significant portion of the carbon dioxide fixed during photosynthesis by terrestrial plants.Nevertheless,with the expansion of the global population and ongoing industrialization,forest coverage has been substantially decreased,resulting in significant challenges for wood production and supply.Wood production practices have changed away from natural forests toward plantation forests.Thus,understanding the underlying genetic mechanisms of wood formation is the foundation for developing high-quality,fast-growing plantation trees.Breeding ideal forest trees for wood production using genetic technologies has attracted the interest of many.Tremendous studies have been carried out in recent years on the molecular,genetic,and cell-biological mechanisms of wood formation,and considerable progress and findings have been achieved.These studies and findings indicate enormous possibilities and prospects for tree improvement.This review will outline and assess the cellular and molecular mechanisms of wood formation,as well as studies on genetically improving forest trees,and address future development prospects.

关键词： cambium cell wall Populus wood formation xylem

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prenatal cadmium exposure impairs neural tube closure via inducing excessive apoptosis in neuroepithelium

引用

Journal of Environmental Sciences 2024年第4期138卷 572-584页

作者： Shiyong zhu Xuenan li Xueyan Dai Jinlong li College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150030China Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment Northeast Agricultural UniversityHarbin 150030China Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine Northeast Agricultural UniversityHarbin 150030China

Birth defects have become a public health concern.The hazardous environmental factors exposure to embryos could increase the risk of birth defects.Cadmium,a toxic environmental factor,can cross the placental barrier during pregnancy.Pregnant woman may be subjected to cadmium before taking precautionary protective actions.However,the link between birth defects and cadmium remains obscure.Cadmium exposure can induce excessive apoptosis in neuroepithelium during embryonic development progresses.Cadmium exposure activated the p53 via enhancing the adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPK)and reactive oxygen species'(ROS)level.And cadmium decreases the level of Paired box 3(Pax3)and murine double minute 2(Mdm2),disrupting the process of p53 ubiquitylation.And p53 accumulation induced excessive apoptosis in neuroepithelium during embryonic development progresses.Excessive apoptosis led to the failure of neural tube closure.The study emphasizes that environmental materials may increase the health risk for embryos.Cadmium caused the failure of neural tube closure during early embryotic day.Pregnant women may be exposed by cadmium before taking precautionary protective actions,because of cadmium concentration-containing foods and environmental tobacco smoking.This suggests that prenatal cadmium exposure is a threatening risk factor for birth defects.

关键词： Cadmium Neural tube closure Excessive apoptosis p53 Embryogenesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：