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World Journal of Gastroenterology 2015年第22期21卷 6872-6883页

作者： In-Kyung Lee Seoung-Ae Lee Hong Kim you-sub won Bum-Joon Kim Department of Microbiology and Immunology Liver Research Institute Cancer Research Institute and SNUMRCCollege of Medicine Seoul National University

AIM: To investigate the mechanism of endoplasmic reticulum(ER) stress induction by an occult infection related hepatitis B virus S surface antigen(HBsAg)***: We used an HBsAg variant with lower secretion capacity, which was a KD variant from a Korean subject who was occultly infected with the genotype C. We compared the expression profiles of ER stress-related proteins between HuH-7 cells transfected with HBsAg plasmids of a wild-type and a KD variant using Western ***: Confocal microscopy indicated that the KD variant had higher levels of co-localization with ER than the wild-type HBsAg. The KD variant upregulated ER stress-related proteins and induced reactive oxygen species(ROS) compared to the wildtype via an increase in calcium. The KD variant also down-regulated anti-oxidant proteins(HO-1, catalase and SOD) compared to the wild-type, which indicates positive amplification loops of the ER-ROS axis. The KD variant also induced apoptotic cell death via the upregulation of caspase proteins(caspase 6, 9 and 12).Furthermore, the KD variant induced a higher level of nitric oxide than wild-type HBsAg via the up-regulation of the iNOS ***: Our data indicate that occult infection related HBsAg variants can lead to ER-derived oxidative stress and liver cell death in HuH-7 cells.

关键词： Endoplasmic reticulum oxidative stress Hepatitis B virus KD variant Colocalization Reactive oxidative species Apoptotic cell death

Occult infection related hepatitis B surface antigen variants showing lowered secretion capacity

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World Journal of Gastroenterology 2015年第6期21卷 1794-1803页

作者： Hong Kim Seoung-Ae Lee you-sub won Hyun Joo Lee Bum-Joon Kim Department of Biomedical Sciences Microbiology and Immunology and Liver Research Institute Seoul National University College of Medicine

AIM:To elucidate the molecular mechanisms underlying hepatitis B virus(HBV)occult infection of genotype ***:A total of 10 types of hepatitis B surface antigen(HBs Ag)variants from a Korean occult cohort were *** a complete HBV genome plasmid mutated such that it does not express HBs Ag and plasmid encoding,each HBs Ag variant was transiently co-transfected into Hu H-7 *** secretion capacity and intracellular expression of the HBV virions and HBs Ags in their respective variants were analyzed using real-time quantitative polymerase chain reaction assays and commercial HBs Ag enzyme-linked immunosorbent assays,***:All variants exhibited lower levels of HBs Ag secretion into the medium compared with the wild *** particular,in eight of the ten variants,very low levels of HBs Ag secretion that were similar to the negative control were *** contrast,most variants(9/10)exhibited normal virion secretion capacities comparable with,or even higher than,the wild *** provided new insight into the intrinsic nature of occult HBV infection,which leads to HBs Ag sero-negativeness but has horizontal ***,most variants generated higher reactive oxidative species production than the wild *** finding provides potential links between occult HBV infection and liver disease ***:The presently obtained data indicate that deficiency in the secretion capacity of HBs Ag variants may have a pivotal function in the occult infections of HBV genotype C.

关键词： Occult infection Hepatitis B virus Hepatitis B sur

Effects of multi-dopants （Zn and Ho） in stabilizing spinel structure for cathode materials in lithium rechargeable batteries-Novel chelated sol-gel synthesis

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Particuology 2016年第1期14卷 87-95页

作者： R. Thirunakaran Taewhan Kim won-sub Yoon CSIR-Central Electrochemical Research Institute Karaikudi 630 006 Tamil Nadu India Department of Energy Science Sungkyunkwan University Suwon 440-746 South Korea

Multi-doped spinels, namely LiMn204 and LiZnxHoyMn2 x yO4 （x=0.10-0.18; y= 0.02-0.10）, for use as cathode materials for lithium-ion rechargeable batteries were synthesized via sol-gel method, using lauric acid as the chelating agent, to obtain micron-sized particles. The physical properties of the synthesized samples were investigated using differential thermal analysis, Fourier-transform infrared spectroscopy, X-ray diffraction （XRD）, scanning electron microscopy （SEM）, transmission electron microscopy, energy-dispersive X-ray analysis, and electrochemical methods. XRD showed that LiMn204 and LiZnxHoyMn2_x y04 have high degrees of crystallinity and good phase purities. The SEM images of LiMn204 showed an ice-cube morphology with particles of size 1 μm. Charge-discharge studies showed that undoped LiMn2 O4 delivered the discharge capacity of 124 mA h/g with coulombic efficiency of 95% during the first cycle, whereas doped spinels delivered discharge capacities of 125, 120, and 127 mA h/g in the first cycle with coulombic efficiencies of 96%, 91%, and 91%, respectively.

关键词： Holmium Zinc Lauric acid Sol-gel Spinel cathode Lithium-ion battery

Synthesis and electrochemical properties of dual doped spinels LiNi_xAl_yMn_(2-x-y)O_4 via facile novel chelated sol–gel method as possible cathode material for lithium rechargeable batteries

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Journal of Energy Chemistry 2017年第1期26卷 101-114页

作者： R.Thirunakaran Gil Hwan Lew won-sub Yoon CSIR-Central Electrochemical Research Institute Sungkyunkwan University Department of Energy Science

LiMnOand LiNiAlyMnO（x= 0.50;y = 0.05-0.50） powders have been synthesized via facile solgel method using Behenic acid as active cheiating *** synthesized samples are subjected to physical characterizations such as thermo gravimetric analysis（TG/DTA）,X-ray diffraction（XRD）,Fourier transform infrared spectroscopy（FT-IR）,field-emission scanning electron microscopy（FESEM）,transmission electron microscopy（TEM） and electrochemical studies viz.,galvanostatic cycling properties,electrochemical impedance spectroscopy（EIS） and differential capacity curves（dQ/dE）.Finger print XRD patterns of LiMnOand LiNiAlMnOfortify the high degree of crystallinity with better phase *** images of the undoped pristine spinel illustrate uniform spherical grains surface morphology with an average particle size of 0.5 μm while Ni doped particles depict the spherical grains growth（50nm） with ice-cube surface *** images of the spinel LiMnOshows the uniform spherical morphology with particle size of（100 nm） while low level of Al-doping spinel（LiNio.5Alo.05Mn1.45O4） displaying cloudy particles with agglomerated particles of（50nm）.The LiMnOsamples calcined at 850℃ deliver the discharge capacity of 130 mAh/g in the first cycle corresponds to 94%coiumbic efficiency with capacity fade of 1.5 mAh/g/cycle over the investigated 10 *** all four dopant compositions investigated,LiNiAlMnOdelivers the maximum discharge capacity of 126 mAh/g during the first cycle and shows the stable cycling performance with low capacity fade of 1 mAh/g/cycle（capacity retention of 92%） over the investigated 10 *** impedance studies of spinel LiMnOand LiNiAlMnOdepict the high and low real polarization of 1562 and 1100 Ω.

关键词： Multi-doping Sol–gel method Behenic acid Differential capacity Spinel cathode

Application of automated cone penetrometer for railway investigation using correlations with DCPI and deflection modulus

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Research in Cold and Arid Regions 2022年第4期14卷 235-238页

作者： Sang Yeob Kim won-Taek Hong Jong-sub Lee School of Civil Environmental and Architectural EngineeringKorea UniversitySeoul 02841Republic of Korea Department of Civil&Environmental Engineering Gachon UniversitySeongnam-si 13120Republic of Korea

Portable in-situ devices have been used for characterizing low accessible field,such as the railway *** this study,the automated cone penetrometer(ACP)was designed for the application on the railway *** is composed of the cone tip,driving rod,and hydraulic hammer *** hydraulic motor lifts and drops the 294.3 N hammer from a height of 200 mm such that the potential energy of 58.9 N m impacts the driving *** N-value(NACP)from the ACP test was compared with the dynamic cone penetration index(DCPI)from the dynamic cone penetrometer(DCP)*** test results show that the NACP and DCPI profiles show opposite trend owing to the inverse concept of the *** the correlation of DCPI and NACP,the limitation of DCPI reveals owing the minimum manually measured value of 1 mm/***,the evaluation of the deflection modulus(EFWD)using NACP is more efficient than that using *** on the result of this study,we suggest that ACP can be used for strength and stiffness evaluation of railway subgrade rapidly and reliably.

关键词： Automated cone penetrometer DCPI E_(FWD )N_(ACP) Railway subgrade

Reaction mechanism and additional lithium storage of mesoporous MnO_(2) anode in Li batteries

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Journal of Energy Chemistry 2021年第2期30卷 276-284,I0010页

作者： Jaesang Yoon Woosung Choi Taewhan Kim Hyunwoo Kim Yun Seok Choi Ji Man Kim won-sub Yoon Department of Energy Science Sungkyunkwan UniversitySuwon 16419South Korea Department of Chemistry Sungkyunkwan UniversitySuwon 16419South Korea

Nanostructured transition metal oxides,employed as anode materials for lithium-ion batteries,exhibit a higher capacity than the theoretical capacity based on the conversion *** date,the reasons behind this phenomenon are *** the one-step evolution of anode material for lithium-ion batteries,it is essential to understand the lithium storage reaction mechanism of the anode ***,we provide a detailed report on the lithium storage and release mechanism of MnO2,using synchrotron-based X-ray techniques.X-ray diffraction and X-ray absorption spectroscopy results indicate that during the first discharge,MnO2 is reduced in the order of MnO2→LixMnO2(1

关键词： Mesoporous MnO_(2) Reaction mechanism Electrolyte-derived surface layer Synchrotron-based X-ray techniques Anode Li battery

Hybrid functional IrO_2-TiO_2 thin film resistor prepared by atomic layer deposition for thermal inkjet printheads

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Transactions of Nonferrous Metals Society of China 2011年第A1期21卷 88-91页

作者： won-sub KWACK Hyoung-Seok MOON Seong-Jun JEONG Qi-min WANG Se-Hun Kwon National Core Research Center for Hybrid Materials Solution Pusan National University Busan 609-735 Korea Department of Materials Science and Engineering Korea Advanced Institute of Science and Technology Daejon 373-1 Korea

IrO2-TiO2 thin films were prepared by atomic layer deposition using Ir（EtCp）（COD） and titanium isopropoxide （TTIP）. The resistivity of IrO2-TiO2 thin films can be easily controlled from 1 500 to 356.7 μΩ·cm by the IrO2 intermixing ratio from 0.55 to 0.78 in the IrO2-TiO2 thin films. The low temperature coefficient of resistance（TCR） values can be obtained by adopting IrO2-TiO2 composite thin films. Moreover, the change in the resistivity of IrO2-TiO2 thin films was below 10% even after O2 annealing process at 600 ℃. The step stress test results show that IrO2-TiO2 films have better characteristics than conventional TaN08 heater resistor. Therefore, IrO2-TiO2 composite thin films can be used as a heater resistor material in thermal inkjet printhead.

关键词： IrO2-TiO2 film heating resistor inkjet

Application of a nondestructive method to evaluate the active layer in a cold region

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Research in Cold and Arid Regions 2017年第3期9卷 213-220页

作者： won-Taek Hong Jong-sub Lee School of Civil Environmental and Architectural Engineering Korea University

To provide a safe transportation system in an extremely cold region,evaluation needs to be conducted of the thickness and the volumetric water content of the active layer,as they significantly affect frost *** objective of this study was to evaluate the dielectric constant(κ)of the active layer using ground-penetrating radar(GPR)and a dynamic cone penetrometer(DCP);this evaluation was then used to estimate the thickness and the volumetric water content of the active layer.A field located in midwest Alaska was selected as the study site.A GPR survey and two DCP tests were conducted on the surface of the ground,and the ground temperature was *** the GPR survey,travel times of the electromagnetic wave in the active layer were *** addition,the thickness of the active layer was determined by using the dynamic cone penetration index(DCPI)and ground *** using the travel time and travel distance of the electromagnetic wave in the active layer,dielectric constants were calculated as 26.3 and 26.4 for two DCP *** the mean dielectric constant,the volumetric water content was estimated to be 40%~43%,and the thickness of the active layer was evaluated along the GPR survey *** spatial-scaled GPR image showed that the thickness of the active layer varied from 520 mm to 700 mm due to the presence of a puddle,which accelerated the heat *** results show that evaluation of the dielectric constant using the GPR survey and the DCP test can be effectively used to estimate the thickness and the volumetric water content of the active layer.

关键词： active layer dielectric constant dynamic cone penetrometer (DCP) extremely cold region ground-penetrating radar (GPR)

Hepatitis B virus pre S1 deletion is related to viral replication increase and disease progression

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World Journal of Gastroenterology 2015年第16期21卷 5039-5048页

作者： Seoung-Ae Lee Ki-Jeong Kim Hong Kim won-Hyuk Choi Yu-sub won Bum-Joon Kim Department of Biomedical Sciences Microbiology and Immunologyand Liver Research InstituteSeoul National University College of Medicine Department of Microbiology School of MedicineChung-Ang University Department of Internal Medicine Konkuk University

AIM:To investigate the clinical implications of hepatitis B virus(HBV) pre S1 ***:We developed a fluorescence resonance energy transfer-based real-time polymerase chain reaction(RT-PCR) that can detect four genotypes(wild type, 15-bp, 18-bp and 21-bp deletion).The PCR method was used in two cohorts of Korean chronic HBV subjects with genotype C *** Ⅰ included 292 chronic HBV subjects randomly selected from Cheju National University Hospital(Jeju, South Korea) or Seoul National University Hospital(Seoul, South Korea), and cohort Ⅱ included 90 consecutive chronic HBV carriers recruited from Konkuk University Hospital(Seoul, South Korea); the cohort Ⅱ patients did not have hepatocellular carcinoma or liver ***:The method proposed in this study identified 341 of 382 samples(89.3%).Deletion variants were identified in 100(29.3%) of the 341 detected *** both cohorts, the subjects with deletions had a significantly higher Hepatitis B virus e antigen(HBe Ag)-positive seroprevalence [cohort Ⅰ, wild(51.0%) vs deletion(75.0%), P subjects with wild type ***:HBV genotype C pre S1 deletion may affect disease progression in chronic HBV subjects through an extended duration of HBe Ag seropositive status and increased HBV replications.

关键词： Hepatitis B virus PreS1 start codon deletion Hepatitis B virus e antigen Hepatocellular carcinoma Genotype C

Inhomogeneous lithium-storage reaction triggering the inefficiency of all-solid-state batteries

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Journal of Energy Chemistry 2022年第3期31卷 226-236,I0008页

作者： Jaeyoung Kim wontae Lee Jangwhan Seok Eunkang Lee Woosung Choi Hyunyoung Park Soyeong Yun Minji Kim Jun Lim won-sub Yoon Department of Energy Science Sungkyunkwan UniversitySuwon 16419South Korea Pohang Accelerator Laboratory Pohang University of Science and TechnologyPohang 37673South Korea

All-solid-state batteries offer an attractive option for developing safe lithium-ion *** the various solid-state electrolyte candidates for their applications,sulfide solid electrolytes are the most suitable owing to their high ionic conductivity and facile ***,their performance is extensively lower compared with those of conventional liquid electrolyte-based batteries mainly because of interfacial reactions between the solid electrolytes and high capacity ***,the kinetic evolution reaction in the composite cathode of all-solid-state lithium batteries has not been actively ***,electrochemical analyses were performed to investigate the differences between the organic liquid electrolyte-based battery and all-solid-state battery *** with electrochemical analyses and synchrotron-based in situ and ex situ X-ray analyses,it was confirmed that inhomogeneous reactions were due to physical *** contacted and/or isolated active material particles account for the inhomogeneously charged regions,which further intensify the inhomogeneous reactions during extended cycles,thereby increasing the polarization of the *** study highlighted the benefits of electrochemo-mechanical integrity for securing a smooth conduction pathway and the development of a reliable homogeneous reaction system for the success of solid-state batteries.

关键词： Liquid electrolyte lithium batteries All-solid-state lithium batteries Ni-rich cathode Synchrotron-based X-ray techniques Inhomogeneous reaction