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Nuclear factor kappa B: A marker of chemotherapy for human stage Ⅳ gastric carcinoma

World Journal of Gastroenterology 2008年第30期14卷 4739-4744页

作者： Sheng Ye you-ming long Jian Rong Wen-Rui Xie Oncology Department the First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Clinical College Guangdong Pharmaceutical University Guangzhou 510080 Guangdong Province China Jian Rong Department of Anesthesiology the First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 Guangdong Province China the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou 510080 Guangdong Province. China

AIM： To detect the nuclear factor kappa B （NF-κB） condition in human stage IV gastric carcinoma patients and to explore the correlation between NF-κB activation and survival of these patients after chemotherapy. METHODS： Expression of NF-κB-p65 was determined by immunohistochemical analysis. Activity of NF-κB DNA-binding in carcinoma tissue was detected by electrophoretic mobility shift assay. Kaplan-Meier survival analysis was performed to show the relation between NF-κB and progression-free survival （PFS） or overall survival （OS） of the patients. RESULTS： The positive expression rate of NF-κB-p65 in 60 gastric cancer tissue samples was 76.7% （46160）. The expression of NF-κB-p65 was reduced in adjacent carcinoma and normal tissue samples. Electrophoretic mobility shift assay （EMSA） analysis showed a strong activation of NF-κB in cancer tissue samples. A survival difference was found in NF-κB-p65 positive and negative patients. NF-κB-p65 expression was negative in cancer tissue samples （n = 14）. PFS was 191.40 ± 59.88 d and 152.93 ±16.99 d, respectively, in patients with positive NF-κB-p65 expression （n = 46）（P = 0.4028）. The survival time of patients with negative and positive NF-κB-p65 expression was 425.16 ±61.61 d and 418.85 ±42.98 d, respectively （P = 0.7303）. Kaplan-Meier analysis showed no significant difference in PFS or OS. The 46 patient tissue which positive NF-κB-p65 expression was found in the tissue samples from the 46 patients whose PFS and OS were 564.89 ± 75.94 d and s 352.37 ±41.32 d, respectively （P = 0.0165）. CONCLUSION： NF-κB is activated in gastric carcinoma tissue, which is related to the OS after chemotherapy.

关键词： Gastric carcinoma Nuclear factor kappa B Activation Survival analysis Therapy

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Cell-permeable Tat-NBD peptide attenuates rat pancreatitis and acinus cell inflammation response

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World Journal of Gastroenterology 2009年第5期15卷 561-569页

作者： you-ming long Ken Chen Xue-Jin Liu Wen-Rui Xie Hui Wang College of Medicine Guangdong Pharmaceutical University Guangzhou 510080 Guangdong Province China Clinical and Pharmaceutical Research Unit Guangdong Pharmaceutical University Guangzhou 510310Guangdong Province China Department of Digestion Zhoukou Central Hospital of Henan Zhoukou 46600 Henan Province China First Affiliated Hospital of Guangdong Pharmaceutical' University Guangzhou 510080 Guangdong Province China Guangdong Pharmaceutical University Guangzhou 510006 Guangdong Province China

AIM： To investigate the effects of Tat-NEMO-binding domain （NBD） peptide on taurocholate-induced pancreatitis and lipopolysaccharide （LPS）-stimulated AR42J acinus ceils inflammatory response in rats. METHODS： Sodium taurocholate （5%） was used to induce the pancreatitis model. Forty-eight rats from the taurocholate group received an intravenous bolus of 13 mg/kg Tat-NBD （wild-type, WT） peptide, Tat- NBD （mutant-type, MT） peptide, NBD peptide or Tat peptide. The pancreatic histopathology was analyzed by hematoxylin staining. LPS was added to the culture medium to stimulate the AR42J cells. For pretreatment, cells were incubated with different peptides for 2 h before LPS stimulation. Expression of IL-1β and TNF-α mRNA was analyzed using a semi-quantitative reverse-transcript polymerase chain reaction （RT-PCR） method. IL-1β and TNF-α protein in culture medium were detected by enzyme linked immunosorbent assay （ELISA）. NF-KB DNA-binding in pancreas was examined by electrophoretic mobility shift assays. P65 expression of AR42J was determined by Strept Actividin-Biotin Complex （SABC） method. RESULTS： Pretreatment with Tat-NBD （WT） peptide at a concentration of 13 mg/kg body wt showed beneficial effect in pancreaitis model. LPS （10 mg/L） resulted in an increase of IL-1β mRNA, IL-1β protein, TNF-α mRNA and TNF-α protein, whereas significantly inhibitory effects were observed when cells were incubated with Tat-NBD （WT）. Consisting with p65 expression decrease analyzed by SABC method, NF-KB DNA-binding activity significantly decreased in Tat-NBD （WT） pretreatment group, especially at the largest dose. No significant changes were found in the control peptide group. CONCLUSION： Our result supports that active NF-KB participates in the pathogenesis of STC-induced acute pancreatitis in rats. Tat-NBD （WT） peptide has anti- inflammatory effects in this model and inhibits the inflammation of acinus simulated by LPS.

关键词： Pancreatitis Nuclear factor kappa B Cytokine Peptide Pretreatment

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急性胰腺炎细胞凋亡机制研究进展

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世界华人消化杂志 2009年第6期17卷 594-597页

作者：石星亮陈垦王晖龙友明广东药学院临床医学院内科学教研室广东省广州市510310 广东药学院中药学院药理学教研室广东省广州市510006

急性胰腺炎(acute pancreatitis,AP)是临床上常见的危重疾病.该病的发病机制复杂,以往研究多集中在炎症因子、氧化应激、微循环障碍等方面.近年人们发现细胞凋亡在急性胰腺炎的发生发展过程中起重要作用,他参与了急性胰腺炎的组织损伤,... 详细信息

急性胰腺炎(acute pancreatitis,AP)是临床上常见的危重疾病.该病的发病机制复杂,以往研究多集中在炎症因子、氧化应激、微循环障碍等方面.近年人们发现细胞凋亡在急性胰腺炎的发生发展过程中起重要作用,他参与了急性胰腺炎的组织损伤,但同时也是机体其中一种重要的自身保护机制.本文简述细胞凋亡与急性胰腺炎之间的关系,探讨细胞凋亡对急性胰腺炎器官损伤的影响,分析细胞凋亡与炎症因子、氧化应激、内质网应激、Notch因子的关系,从而揭示急性胰腺炎细胞凋亡的发生机制.

关键词：急性胰腺炎细胞凋亡机制炎症因子氧化应激内质网应激

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MoS_(2)Lubricate‑Toughened MXene/ANF Composites for Multifunctional Electromagnetic Interference Shielding

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Nano-Micro Letters 2025年第2期17卷 358-371页

作者： Jiaen Wang Wei ming longfu Chen Tianliang Song Moxi Yele Hao Zhang long Yang Gegen Sarula Benliang Liang Luting Yan Guangsheng Wang School of Physical Science and Engineering Beijing Jiaotong UniversityBeijing 100044People’s Republic of China Key Laboratory of Bio‑Inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191People’s Republic of China

The design and fabrication of high toughness electromagnetic interference(EMI)shielding composite films with diminished reflection are an imperative task to solve electromagnetic pollution *** MXene/ANF(aramid nanofibers)–MoS_(2)composite films with nacre-like layered structure here are fabricated after the introduction of MoS_(2)into binary MXene/ANF composite *** introduction of MoS_(2)fulfills an impressive“kill three birds with one stone”improvement effect:lubrication toughening mechanical performance,reduction in secondary reflection pollution of electromagnetic wave,and improvement in the performance of photothermal *** the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 50:50),the strain to failure and tensile strength increase from 22.1±1.7%and 105.7±6.4 MPa and to 25.8±0.7%and 167.3±9.1 MPa,*** toughness elevates from 13.0±4.1 to 26.3±0.8 MJ m^(−3)(~102.3%)*** the reflection shielding effectiveness(SE_(R))of MXene/ANF(mass ratio of 50:50)decreases~10.8%.EMI shielding effectiveness(EMI SE)elevates to 41.0 dB(8.2–12.4 GHz);After the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 60:40),the strain to failure increases from 18.3±1.9%to 28.1±0.7%(~53.5%),the SE_(R)decreases~22.2%,and the corresponding EMI SE is 43.9 *** MoS_(2)also leads to a more efficient photothermal conversion performance(~45 to~55℃).Additionally,MXene/ANF–MoS_(2)composite films exhibit excellent electric heating performance,quick temperature elevation(15 s),excellent cycle stability(2,2.5,and 3 V),and long-term stability(2520 s).Combining with excellent mechanical performance with high MXene content,electric heating performance,and photothermal conversion performance,EMI shielding ternary MXene/ANF–MoS_(2)composite films could be applied in many industrial *** work broadens how to achieve a balance between mechanical properties and versatility of composites in the case of high-function fillers.

关键词： MXene-MoS_(2) Lubrication toughening EMI shielding Photothermal conversion Electric heating performance

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PTD-NBD多肽对大鼠胰腺腺泡细胞炎性反应的抑制作用

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世界华人消化杂志 2009年第19期17卷 1931-1935页

作者：刘学进龙友明陈垦谢文瑞王晖河南省周口市中心医院消化内科河南省周口市466000 广东药学院临床学院广东省广州市510080 广东药学院中药学院广东省广州市510080

目的:探讨透细胞性PTD-NBD多肽对脂多糖诱导的炎性效应的干预与作用机制.方法:以脂多糖刺激大鼠胰腺腺泡细胞AR42J,构建急性胰腺炎的体外模型.不同浓度免疫缺陷性病毒PTD多肽蛋白转导功能区与野生型NBD多肽融合成PTD-NBD多肽,对AR42J细... 详细信息

目的:探讨透细胞性PTD-NBD多肽对脂多糖诱导的炎性效应的干预与作用机制.方法:以脂多糖刺激大鼠胰腺腺泡细胞AR42J,构建急性胰腺炎的体外模型.不同浓度免疫缺陷性病毒PTD多肽蛋白转导功能区与野生型NBD多肽融合成PTD-NBD多肽,对AR42J细胞进行预处理,突变型PTD-NBD(MT)多肽、PTD、NBD为对照.RT-PCR法观察ICAM-1及IL-1βmRNA的表达;定量酶联免疫吸附法检测培养液上清中IL-1β蛋白浓度的改变.结果:透细胞性PTD-NBD多肽可以抑制脂多糖诱导的AR42J炎症细胞因子ICAM-1和IL-1βmRNA及蛋白的表达,且呈剂量依赖方式.PTD-NBD(WT)多肽与单纯NBD多肽、突变型PTD-NBD(MT)多肽及PTD组比较(相对灰度值:0.449±0.088vs1.132±0.069,1.158±0.095,1.206±0.078),能够抑制ICAM-1mRNA表达.上述4组的IL-1βmRNA相对灰度值分别为0.526±0.077,0.993±0.065,1.143±0.086和1.128±0.117,IL-1β蛋白表达分别为278.82±61.80ng/L、898.08±74.65ng/L、945.25±42.49ng/L和947.86±38.66ng/L,结果显示后3组不能抑制IL-1βmRNA及其蛋白的表达.结论:PTD-NBD(WT)多肽可以呈剂量依赖方式抑制LPS诱导的AR42J促炎细胞因子IL-1β和ICAM-1的表达,其可直接影响胰腺腺泡细胞炎性反应.

关键词：急性胰腺炎脂多糖细胞因子腺泡细胞多肽

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核因子-κB在急性胰腺炎中的激活及NBD多肽的干预作用

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世界华人消化杂志 2008年第18期16卷 1980-1984页

作者：谢文瑞陈垦阳小雅龙友明王晖刘君君杨剑广东药学院附属第一医院消化内科广东省广州市510080 广东药学院临床医学院广东省广州市510310 广州医学院护理学院广东省广州市510180

目的:探讨核因子-κB(nuclear factor-kappa B,NF-κB)及活性氧簇(reactive oxygen species,ROS)在大鼠SAP发病过程中的作用;并观察NF-κB必需调节蛋白结合域(NF-κB essentiamodifier binding domain,NBD)多肽对大鼠SAP的干预作用.方法:逆行胰胆管内注射50g/L牛磺胆酸钠(1mL/kg)制备大鼠SAP模型.大鼠64只,随机分成4组:假手术组,SAP组,TAT-NBD(WT)多肽组和TAT-NBD(MT)多肽组.术后6,12h处死大鼠,观察胰腺组织病理、血清淀粉酶和胰腺组织NF-κBP65蛋白的变化,检测胰腺组织MDA含量及T-SOD活力.结果:与假手术组比较,SAP模型组6,12h大鼠NF-κBP65蛋白表达显著升高(49.3±2.2 vs 4.3±1.4,65.8±1.8 vs 5.0±1.3,均P<0.05)MDA生成增加(212.7±12.5 vs 87.7±7.5296.8±13.3 vs 96.2±8.3,均P<0.05),T-SOD活力下降(88±10 vs 183±10,65±7 vs 194±10均P<0.05);与SAP模型组比较,TAT-NBD(WT多肽预处理6,12h后,NF-κBP65蛋白表达(25.9±2.3,38.9±2.6)显著降低(P<0.05),MDA生成(102.5±10.4,164.5±12.2)降低(P<0.05)T-SOD活力(153±11,168±12)增加(P<0.05)胰腺病理学评分下降(5.04±0.41 vs 8.71±0.45,5.45±0.34 vs 10.31±1.23,均P<0.05),淀粉酶无明显变化.TAT-NBD(MT)多肽组上述指标均无明显变化.结论:SAP时大鼠胰腺组织的NF-κB过度激活,活性氧簇生成增加;NBD多肽预处理可以抑制NF-κB过度活化,减少活性氧簇生成,减轻胰腺局部炎症损伤.

关键词：重症急性胰腺炎核因子-κB 多肽大鼠活性氧簇

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Experimental study of the mechanism of nanofluid in enhancing the oil recovery in low permeability reservoirs using microfluidics

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Petroleum Science 2023年第1期20卷 382-395页

作者： Kang Wang Qing you Qiu-ming long Biao Zhou Pan Wang School of Energy Resources China University of GeosciencesBeijing100083China School of Petroleum Engineering China University of PetroleumQingdao266580ShandongChina Chengdu Natural Gas Chemical General Factory Chengdu610213SichuanChina

Due to the low porosity and low permeability in unconventional reservoirs,a large amount of crude oil is trapped in micro-to nano-sized pores and throats,which leads to low oil *** have great potential to enhance oil recovery(EOR)in low permeability *** this work,the regulating ability of a nanofluid at the oil/water/solid three-phase interface was *** results indicated that the nanofluid reduced the oil/water interfacial tension by two orders of magnitude,and the expansion modulus of oil/water interface was increased by 77% at *** addition,the solid surface roughness was reduced by 50%,and the three-phase contact angle dropped from 135(oil-wet)to 48(water-wet).Combining the displacement experiments using a 2.5D reservoir micromodel and a microchannel model,the remaining oil mobilization and migration processes in micro-to nano-scale pores and throats were *** was found that the nanofluid dispersed the remaining oil into small oil droplets and displaced them via multiple mechanisms in porous ***,the high strength interface film formed by the nanofluid inhibited the coalescence of oil droplets and improved the flowing *** results help to understand the EOR mechanisms of nanofluids in low permeability reservoirs from a visual perspective.

关键词： Nanofluid EOR mechanism Micromodel Low permeability Three-phase interface

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Advances of surface-enhanced Raman and IR spectroscopies: from nano/microstructures to macro-optical design

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Light(Science & Applications) 2021年第9期10卷 1588-1606页

作者： Hai-long Wang En-ming you Rajapandiyan Panneerselvam Song-Yuan Ding Zhong-Qun Tian State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy MaterialsCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen361005China Department of Chemistry SRM University-APAmaravathiAndhra PradeshIndia

Raman and infrared(IR)spectroscopy are powerful analytical techniques,but have intrinsically low detection *** have been three major steps(i)to advance the optical system of the light excitation,collection,and detection since 1920s,(ii)to utilize nanostructure-based surface-enhanced Raman scattering(SERS)and surface-enhanced infrared absorption(SEIRA)since 1990s,and(iii)to rationally couple(i)and(ii)for maximizing the total detection sensitivity since *** surveying the history of SERS and SEIRA,we outline the principle of plasmonics and the different mechanisms of SERS and *** describe various interactions of light with nano/microstructures,localized surface plasmon,surface plasmon polariton,and lightning-rod *** coupling effects can significantly increase the surface sensitivity by designing nanoparticle–nanoparticle and nanoparticle–substrate *** the nano/microstructures have specific optical near-field and far-field behaviors,we focus on how to systematically design the macro-optical systems to maximize the excitation efficiency and detection *** enumerate the key optical designs in particular ATR-based operation modes of directional excitation and emission from visible to IR spectral *** also present some latest advancements on scanning-probe microscopy-based nanoscale ***,prospects and further developments of this field are given with emphasis on emerging techniques and methodologies.

关键词： surface excitation utilize

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Photochemical Selective Desilylation of t-Butyldimethylsilyl Ethers

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Chinese Chemical Letters 2001年第2期12卷 95-98页

作者： Mei Zhong JIN Li YANG long ming WU you Cheng LIU Zhong Li LIU National Laboratory of Applied Organic Chemistry Lanzhou UniversityLanzhou 730000

A photochemical desilylation reaction with nearly quantilative yield and excellent selectivity between silyl alkyl ether and silyl enol ether is reported.

关键词： photoinduced electron transfer selective desilylation

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Photochemical Reduction of Aromatic Imines by 2-Phenyl-N, N-dimethylbenzimidazoline

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Chinese Chemical Letters 2001年第4期12卷 307-308页

作者： Mei Zhong JIN Li YANG long ming WU you Chen LIU Zhong Li LIU National Laboratory of Applied Organic Chemistry Lanzhou UniversityLanzhou 730000

A series of aromatic imines were reduced to corresponding amines in excellent yields by 2-phenyl-N. N-dimethylbenzimidazoline (PDMBI) photochemically in the presence of magnesium perchloride (Mg(ClO4)(2)). A Mg2+ medi... 详细信息

关键词： Photoinduced electron transfer selective reduction imine PDMBI

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