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Antioxidant Defense Mechanisms of Salinity Tolerance in Rice Genotypes

Rice science 2017年第3期24卷 155-162页

作者： Mohammad Golam KIBRIA Mahmud HOSSAIN yoshiyuki murata Md.Anamul HOQUE Department of Soil Science Bangladesh Agricultural University Graduate School of Natural Science and Technology Okayama University

In order to elucidate the role of antioxidant responses in salinity tolerance in rice genotypes under salt stress, experiments were conducted using four rice varieties, including salt-sensitive BRRI dhan 28 and three salt-tolerant varieties BRRI dhan 47, BINA dhan 8 and BINA dhan 10. Thirty-day-old rice seedlings were transplanted into pots. At the active tillering stage(35 d after transplanting), plants were exposed to different salinity levels(0, 20, 40 and 60 mmol/L NaCl). Salt stress caused a significant reduction in growth for all the rice genotypes. Growth reduction was higher in the salt-sensitive genotype than in the salt-tolerant ones, and BINA dhan 10 showed higher salt tolerance in all measured physiological parameters. The reduction in shoot and root biomass was found to be minimal in BINA dhan 10. Chlorophyll content significantly decreased under salt stress except for BINA dhan 10. Proline content significantly increased in salt-tolerant rice genotypes with increased salt concentration, and the highest proline content was obtained from BINA dhan 10 under salt stress. Catalase and ascorbate peroxidase activities significantly decreased in salt-sensitive genotype whereas significantly increased in salt-tolerant ones with increasing salt concentration. However, salt stress significantly decreased guaiacol peroxidase activity in all the rice genotypes irrespective of salt tolerance. K^+/Na^+ ratio also significantly decreased in shoots and roots of all the rice genotypes. The salt-tolerant genotype BINA dhan 10 maintained higher levels of chlorophyll and proline contents as well as catalase and ascorbate peroxidase activities under salt stress, thus, this might be the underlying mechanism for salt tolerance.

关键词： antioxidant enzyme salinity chlorophyll content proline content K+/Na+ ratio rice

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Detecting Communities in K-Partite K-Uniform (Hyper)Networks

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Journal of Computer Science & Technology 2011年第5期26卷 778-791页

作者：刘欣 Tsuyoshi murata Department of Computer Science Tokyo Institute of Technology

In social tagging systems such as Delicious and Flickr,users collaboratively manage tags to annotate ***,a social tagging system can be modeled as a （user,tag,resource） hypernetwork,where there are three different types of nodes,namely users,resources and tags,and each hyperedge has three end nodes,connecting a user,a resource and a tag that the user employs to annotate the *** how can we automatically cluster related users,resources and tags,respectively？ This is a problem of community detection in a 3-partite,3-uniform *** generally,given a K-partite K-uniform （hyper）network,where each （hyper）edge is a K-tuple composed of nodes of K different types,how can we automatically detect communities for nodes of different types？ In this paper,by turning this problem into a problem of finding an efficient compression of the （hyper）network＇s structure,we propose a quality function for measuring the goodness of partitions of a K-partite K-uniform （hyper）network into communities,and develop a fast community detection method based on *** method overcomes the limitations of state of the art techniques and has several desired properties such as comprehensive,parameter-free,and *** compare our method with existing methods in both synthetic and real-world datasets.

关键词： community detection bipartite graph tripartite hypergraph clustering social tagging

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Ceramide induces apoptosis in human lung adenocarcinoma A549 cells through mitogen-activated protein kinases

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Acta Pharmacologica Sinica 2007年第3期28卷 439-445页

作者： Nobuo murata Tatsuo YAMAKAWA Department of Surgery Mini-invasive surgery CenterTeikyo University Mizonokuchi HospitalKawasaki 213-8507Japan

Aim:To provide experimental data for further research on the signal transductionof apoptosis in lung adenocarcinoma cells,we examined the effects of exogenousC2-ceramide administration on several members of the mitogen-activated proteinkinase(MAPK)superfamily and caspase-3 in A549 ***:Cell viabilityand apoptosis were analyzed by cell counting kit-8 assay and flow *** MAPK and caspase-3 proteins were detected by Western ***:C2-ceramide selectively altered the phosphorylation state &members ofthe MAPK superfamily,causing hyperphosphorylation ofmitogen-activated pro-tein kinase kinase(MEK)1/2 and the p38 MAPK,but not affecting the phosphory-lation ofextracellular signal-regulated kinase 1/2 and the c-Jun N-terminal ***-203580(a p38 MAPK inhibitor)and p38 siRNA,but not U0126(a MEKinhibitor),partially rescued cell death induced by C2-ceramide.C2-ceramide promoted theactivation of ***:Exogenous C2-ceramide induced apoptosisin human lung adenocarcinoma A549 *** activation of MAPK and caspase-3were involved in the mechanisms ofC2-ceramide-induced apoptosis in A549 cells.

关键词： ceramide apoptosis mitogen-activated protein kinases A549 cells

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Applicability of LIVE/DEAD BacLight stain with glutaraldehyde fixation for the measurement of bacterial abundance and viability in rainwater

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Journal of Environmental Sciences 2017年第1期29卷 202-213页

作者： Wei Hu Kotaro murata Daizhou Zhang Faculty of Environmental and Symbiotic Sciences Prefectural University of KumamotoKumamoto 862-8502Japan

Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water ***,quantitative information on rainwater bacteria is very limited due to the lack of a reliable *** this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell *** showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on *** bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater ***,in case of small precipitation,the abundance in negative control could be more than that in rainwater *** results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.

关键词： Bacterial abundance Viability Rainwater LIVE/DEAD BacLight stain Fluorescent enumeration

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Physiological significance of Rag1 in retinal ganglion cell death

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Neural Regeneration Research 2015年第2期10卷 192-194页

作者： Takuma Hayashi Toshinori murata Department of Immunology and Infectious Disease Shinshu University Graduate School of Medicine Department of Ophthalmology Shinshu University Graduate School of Medicine

Although the transcription factor, nudear factor-κB （NF-κB） is known to regulate cell death and survival, its precise role in cell death within the central nervous system （CNS） remains unknown. We previously reported that mice with a homozygous deficiency for NF-κBp50 spon- taneously developed optic neuropathy. We examined the expression and activation of pro-apoptotic factor（s） that mediate optic neuropathy in p50-/- mice. Recombination activating gene 1 （Ragl） is known to regulate the recombination of immunoglobulin V（D）J.

关键词： RGCs Physiological significance of Rag1 in retinal ganglion cell death cell

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An Electronic Approach to Materials Design

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Journal of Materials Science & Technology 2003年第Z1期19卷 73-76页

作者： Masahiko MORINAGA Yoshinori murata Hiroshi YUKAWA Department of Materials Science and Engineering Graduate School of Engineering Nagoya UniversityNagoya 464-8603 Japan Department of Materials Science and Engineering Graduate School of Engineering Nagoya UniversityNagoya 464-8603 Japan Department of Materials Science and Engineering Graduate School of Engineering Nagoya UniversityNagoya 464-8603 Japan

A molecular orbital approach to materials design has recently made great progress. This approach is based onthe electronic structure calculations by the DV-Xα cluster method. In this paper recent progress in this approachis reviewed. In particular, it is stressed that New PHACOMP approach is useful for predicting the formation oftopologically close-packed (TCP) phases (e.g., σ phase andμ phase ) in nickel based superalloys. Compared to thecurrent PHACOMP, New PHACOMP provides a better tool for designing those alloys which are free from such TCPprecipitates at service temperatures. In addition, the d-electrons concept is shown for alloy design and development.

关键词： High temperature materials, Ni based superalloys, Alloy design, Molecular orbital method

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Optimal calibration scheme for map-based control of diesel engines

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Science China(Information Sciences) 2018年第7期61卷 68-81页

作者： Yui NISHIO Yutaka murata Yukihisa YAMAYA Masato KIKUCHI Automobile R & D Center Honda R & D Co. Ltd.

Map-based control has the advantage of a simple control structure. However, in the case of a complex plant, calibrating the map is difficult. Calibration methodology using plant model is increasing in engine calibration industries. However, the models are for steady-state operation and cannot simulate at transient operation well. This study introduces the dynamic empirical model and applies the transient corrective function of an electronic control unit to simulate more realistic behavior. The optimization problem for calibrating maps of diesel engines is constructed, and the formulation of cost function and constraints is discussed. Consequently, the proposed calibration scheme can find an optimal map in satisfied ***, the optimized maps are validated using mass production diesel engines.

关键词： optimization Gaussian SQP Diesel engine calibration DoE

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Remission of bronchial asthma after viral clearance in chronic hepatitis C

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World Journal of Gastroenterology 2005年第47期11卷 7545-7546页

作者： Norihiko Yamamoto Kazumoto murata Takeshi Nakano The First Department of Internal Medicine Mie University School of MedicineTsuMieJapan

A 53-year-old man with a history of blood transfusion at the age of 20 was admitted to our hospital because of liver dysfunction. He had bronchial asthma when he was 18 years old, which naturally resolved within 2 years. However, his bronchial asthma recurred at the age of 45 and was treated with oral theophylline. He was diagnosed as having chronic hepatitis C based on the histological and clinical findings, and then interferon (IFN) therapy was administered. The frequency of bronchial asthma attack was gradually decreasing after IFN therapy with marked improvement of hypereosinophilia. He achieved sustained viral response (SVR) and his bronchial asthma did not worsen even after the cessation of IFN. Hepatitis C virus (HCV) infection and IFN therapy were considered in the remission of asthma in this case. HCV infection could be the cause of bronchial asthma, especially in patients with late appearance of asthma.

关键词： Bronchial asthma Chronic hepatitis C IFN therapy

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Liver microcirculation after hepatic artery embolization with degradable starch microspheres in vivo

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World Journal of Gastroenterology 2006年第26期12卷 4214-4218页

作者： Jian Wang Satoru murata Tatsuo Kumazaki Department of Interventional Radiology and Vascular Surgery The First Hospital of Peking University 100034 Beijing China Department of Radiology Nippon Medical School Tokyo Japan

AIM： To observe the dynamic changes of liver microcirculation in vivo after arterial embolization with degradable starch microspheres （DSM）. METHODS： DSM were injected into the proper hepatic artery through a silastic tube inserted retrogradely in gastroduodenal artery （GDA） of SD rats. Fluorescent microscopy was used to evaluate the dynamic changes of blood flow through the terminal portal venules （TPVs）, sinusoids and terminal hepatic venules （THVs）. The movements of DSM debris were also recorded. Six hours after injection of DSM, percentages of THVs with completely stagnant blood flow were recorded. RESULTS： Two phases of blood flow change were recorded. In phase one： after intra-arterial injection of DSM, slow or stagnant blood flow was immediately recorded in TPVs, sinusoids and THVs. This change was reversible, and blood flow resumed completely. In phase two： after phase one, blood flow in TPVs changed again and three patterns of blood flow were recorded. Six hours after DSM injection, 36.9% ± 9.2% of THVs were found with completely stagnant blood flow. CONCLUSION： DSM can stop the microcirculatory blood flow in some areas of liver parenchyma. Liver parenchyma supplied by arteries with larger A-P shunt is considered at a higher risk of total microcirculatory blood stagnation after injection of DSM through hepatic artery.

关键词： Degradable starch microsphere Hepatic microcirculation Hepatic arteries Fluorescence Transartedal chemoembolization

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Feature selection: Key to enhance node classification with graph neural networks

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CAAI Transactions on Intelligence Technology 2023年第1期8卷 14-28页

作者： Sunil Kumar Maurya Xin Liu Tsuyoshi murata Tokyo Institute of Technology TokyoJapan AIRC AISTTokyoJapan

Graphs help to define the relationships between entities in the *** relationships,represented by edges,often provide additional context information which can be utilised to discover patterns in the *** Neural Networks(GNNs)employ the inductive bias of the graph structure to learn and predict on various *** primary operation of graph neural networks is the feature aggregation step performed over neighbours of the node based on the structure of the *** addition to its own features,for each hop,the node gets additional combined features from its *** aggregated features help define the similarity or dissimilarity of the nodes with respect to the labels and are useful for tasks like node ***,in real-world data,features of neighbours at different hops may not correlate with the node's ***,any indiscriminate feature aggregation by GNN might cause the addition of noisy features leading to degradation in model's *** this work,we show that selective aggregation of node features from various hops leads to better performance than default aggregation on the node classification ***,we propose a Dual-Net GNN architecture with a classifier model and a selector *** classifier model trains over a subset of input node features to predict node labels while the selector model learns to provide optimal input subset to the classifier for the best *** two models are trained jointly to learn the best subset of features that give higher accuracy in node label *** extensive experiments,we show that our proposed model outperforms both feature selection methods and state-of-the-art GNN models with remarkable improvements up to 27.8%.

关键词： classification feature selection neural network

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