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CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice

Asian Journal of Andrology 2022年第3期24卷 266-272页

作者： Yuki Oyama Haruhiko Miyata Keisuke Shimada yoshitaka fujihara Keizo Tokuhiro Thomas X Garcia Martin M Matzuk Masahito Ikawa Graduate School of Pharmaceutical Sciences Osaka UniversitySuitaOsaka 565-0871Japan Department of Experimental Genome Research Research Institute for Microbial DiseasesOsaka UniversitySuitaOsaka 565-0871Japan Department of Bioscience and Genetics National Cerebral and Cardiovascular CenterSuitaOsaka 564-8565Japan Department of Genome Editing Institute of Biomedical ScienceKansai Medical UniversityHirakataOsaka 573-1191Japan Center for Drug Discovery Baylor College of MedicineHoustonTX 77030USA Department of Pathology and Immunology Baylor College of MedicineHoustonTX 77030USA Department of Biology and Biotechnology University of Houston-Clear LakeHoustonTX 77058USA The Institute of Medical Science The University of TokyoMinato-kuTokyo 108-8639Japan

Gene expression analyses suggest that more than 1000–2000 genes are expressed predominantly in mouse and human *** functional analyses of hundreds of these genes have been performed,there are still many testis-enriched genes whose functions remain *** gene function using knockout(KO)mice is a powerful tool to discern if the gene of interest is essential for sperm formation,function,and male fertility in *** this study,we generated KO mice for 12 testis-enriched genes,1700057G04Rik,4921539E11Rik,4930558C23Rik,Cby2,Ldhal6b,Rasef,Slc25a2,Slc25a41,Smim8,Smim9,Tmem210,and Tomm20l,using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)*** designed two gRNAs for each gene to excise almost all the protein-coding regions to ensure that the deletions in these genes result in a null *** tests of KO mice reveal that these 12 genes are not essential for male fertility,at least when individually ablated,and not together with other potentially compensatory paralogous *** results could prevent other laboratories from expending duplicative effort generating KO mice,for which no apparent phenotype exists.

关键词： CRISPR/Cas9 knockout mice male infertility spermatozoa testis

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Ig SF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95

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Bone Research 2020年第1期8卷 97-106页

作者： Hyunsoo Kim Noriko Takegahara Matthew CWalsh Sarah AMiddleton Jiyeon Yu Jumpei Shirakawa Jun Ueda yoshitaka fujihara Masahito Ikawa Masaru Ishii Junhyong Kim Yongwon Choi Department of Pathology and Laboratory Medicine University of Pennsylvania Perelman School of MedicinePhiladelphiaPA 19104USA Department of Biology Department of Computer and Information ScienceSchool of Arts and SciencesProgram in Single Cell BiologyUniversity of PennsylvaniaPhiladelphiaPA 19104USA Research Institute for Microbial Diseases Osaka UniversitySuitaOsaka 565-0871Japan Department of Immunology and Cell Biology Graduate School of Medicine and Frontier BiosciencesOsaka UniversitySuitaOsaka 565-0871Japan

Osteoclasts are multinucleated, giant cells derived from myeloid progenitors. While receptor activator of NF-κB ligand(RANKL)stimulation is the primary driver of osteoclast differentiation, additional signaling further contributes to osteoclast ***, we demonstrate that immunoglobulin superfamily member 11(Ig SF11), whose expression increases during osteoclast differentiation, regulates osteoclast differentiation through interaction with postsynaptic density protein 95(PSD-95), a scaffold protein with multiple protein interaction domains. Ig SF11 deficiency in vivo results in impaired osteoclast differentiation and bone resorption but no observed defect in bone formation. Consequently, Ig SF11-deficient mice exhibit increased bone *** in vitro osteoclast culture systems, we show that Ig SF11 functions through homophilic interactions. Additionally, we demonstrate that impaired osteoclast differentiation in Ig SF11-deficient cells is rescued by full-length Ig SF11 and that the Ig SF11-PSD-95 interaction requires the 75 C-terminal amino acids of Ig SF11. Our findings reveal a critical role for Ig SF11 during osteoclast differentiation and suggest a role for Ig SF11 in a receptor-and signal transduction molecule-containing protein complex.

关键词： SF11 osteoclast impaired

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Spinal cord injury regenerative therapy development:integration of design of experiments

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Neural Regeneration Research 2025年第9期20卷 2571-2573页

作者： Yuji Okano Hideyuki Okano yoshitaka Kase Division of CNS Regeneration and Drug Discovery International Center for Brain Science(ICBS)Fujita Health UniversityKutsukake-ChoToyoake-ShiAichiJapan Department of Extended Intelligence for Medicine The Ishii-Ishibashi LaboratoryKeio University School of MedicineShinanomachiShinjuku-KuTokyoJapan Keio University Regenerative Medicine Research Center Kawasaki-kuKawasaki-shiJapan

Spinal cord injury(SCI)can cause motor and sensory paralysis,and autonomic nervous system disorders including malfunction of urination and defecation,thereby significantly impairing the quality of *** continue to explo re new stem cell strategies for the treatment of paralysis by transpla nting human induced pluripotent stem cell-derived neural ste m/progenitor cells(hiPSCNS/PCs)into spinal cord injured tissues.

关键词： Spinal paralysis injury

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Boosting high-performance in Zr-rich side protonic solid oxide electrolysis cells by optimizing functional interlayer

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Green Energy & Environment 2025年第1期10卷 150-160页

作者： Chunmei Tang Ning Wang Sho Kitano Hiroki Habazaki yoshitaka Aoki Siyu Ye Huangpu Hydrogen Energy Innovation Center School of Chemistry and Chemical EngineeringGuangzhou UniversityNo.230 Wai Huan Xi RoadGuangzhou510006China Graduate School of Chemical Sciences and Engineering Hokkaido UniversityN13W8Kita-kuSapporo060-8628Japan Faculty of Engineering Hokkaido UniversityN13W8Kita-kuSapporo060-8628Japan

Protonic solid oxide electrolysis cells(P-SOECs)are a promising technology for water electrolysis to produce green ***,there are still challenges related key materials and anode/electrolyte interface.P-SOECs with Zr-rich electrolyte,called Zr-rich side P-SOECs,possess high thermodynamically stability under high steam concentrations but the large reaction resistances and the current leakage,thus the inferior *** this study,an efficient functional interlayer Ba_(0.95)La_(0.05)Fe_(0.8)Zn_(0.2)O_(3-δ)(BLFZ)in-between the anode and the electrolyte is *** electrochemical performances of P-SOECs are greatly enhanced because the BLFZ can greatly increase the interface contact,boost anode reaction kinetics,and increase proton injection into *** a result,the P-SOEC yields high current density of 0.83 A cm^(-2) at 600℃ in 1.3 Vamong all the reported Zr-rich side *** work not only offers an efficient functional interlayer for P-SOECs but also holds the potential to achieve P-SOECs with high performances and long-term stability.

关键词： Functional interlayer Zr-rich side electrolyte Protonic solid oxide electrolysis cells Current density Faradaic efficiency

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Outcomes of gastrointestinal defect closure with an overthe-scope clip system in a multicenter experience: An analysis of a successful suction method

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World Journal of Gastroenterology 2017年第9期23卷 1645-1656页

作者： Hideki Kobara Hirohito Mori Shintaro fujihara Noriko Nishiyama Taiga Chiyo Takayoshi Yamada Masao Fujiwara Keiichi Okano Yasuyuki Suzuki Masayuki Murota yoshitaka Ikeda Makoto Oryu Mohamed Abo Ellail Tsutomu Masaki Department of Gastroenterology and Neurology Faculty of MedicineKagawa University Department of Gastroenterology Kochi Health Sciences Center Department of Gastroenterological Surgery Faculty of MedicineKagawa University Department of Gastroenterology Sakaide City Hospital Department of Surgery Ehime Rosai Hospital Department of Gastroenterology Kagawa Saiseikai Hospital Department of Gynecology Faculty of MedicineKagawa University

To demonstrate the clinical outcomes of a multicenter experience and to suggest guidelines for choosing a suction *** retrospective study at 5 medical centers involved 58 consecutive patients undergoing over-the-scope clips (OTSCs) placement. The overall rates of technical success (TSR), clinical success (CSR), complications, and procedure time were analyzed as major outcomes. Subsequently, 56 patients, excluding two cases that used the Anchor device, were divided into two groups: 14 cases of simple suction (SS-group) and 42 cases using the Twin Grasper (TG-group). Secondary evaluation was performed to clarify the predictors of OTSC *** TSR, CSR, complication rate, and median procedure time were 89.7%, 84.5%, 1.8%, and 8 (range 1-36) min, respectively, demonstrating good outcomes. However, significant differences were observed between the two groups in terms of the mean procedure time (5.9 min vs 14.1 min). The CSR of the SS- and TG-groups among cases with a maximum defect size ≤ 10 mm and immediate or acute refractory bleeding was 100%, which suggests that SS is a better method than TG in terms of time efficacy. The CSR in the SS-group (78.6%), despite the technical success of the SS method (TSR, 100%), tended to decrease due to delayed leakage compared to that in the TG-group (TSR, CSR; 88.1%), indicating that TG may be desirable for leaks and fistulae with defects of the entire *** system is a safe and effective therapeutic option for gastrointestinal defects. Individualized selection of the suction method based on particular clinical conditions may contribute to the improvement of OTSC success.

关键词： Over-the-scope clip Leak Gastrointestinal refractory bleeding Fistula Endoscopic closure

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Nonalcoholic steatohepatitis-associated hepatocellular carcinoma:Our case series and literature review

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World Journal of Gastroenterology 2010年第12期16卷 1436-1441页

作者： yoshitaka Takuma Kazuhiro Nouso Department of Gastroenterology Kurashiki Central Hospital Department of Internal Medicine National Hospital Organization Iwakuni Clinical Center Department of Molecular Hepatology Okayama University Graduate School of MedicineDentistryand Pharmaceutical Sciences

Recently,nonalcoholic steatohepatitis(NASH) has been considered to be another cause of liver cirrhosis and hepatocellular carcinoma(HCC).The natural history and prognosis of NASH are ***,we assessed the clinicopathological features of NASH-associated HCC in our experience and reviewed the literature of NASH-associated *** experienced 11 patients with NASH-associated HCC(6 male,5 female;mean age 73.8 ± 4.9 years) who received curative ***(91%) patients had been diagnosed with obesity,diabetes,hypertension,or *** patients(64%) also had a non-cirrhotic *** recurrence-free survival rates at 1,3 and 5 years were 72%,60%,and 60%.We also summarized and reviewed 94 cases of NASH-associated HCC which were reported in the literature(64 male;mean age 66 years).The majority of patients(68%) were obese,66% of patients had diabetes,and 24% had ***,26% of the HCCs arose from the non-cirrhotic *** conclusion,patients with non-cirrhotic NASH may be a high-risk group for HCC,and regular surveillance for HCC is necessary in non-cirrhotic NASH patients as well as cirrhotic patients.

关键词： Nonalcoholic steatohepatitis Hepatocellular carcinoma Nonalcoholic fatty liver disease Cryptogenic cirrhosis

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Quantitative classification of Camellia japonica and Camellia rusticana(Theaceae)based on leaf and flower morphology

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Plant Diversity 2021年第3期43卷 216-224页

作者： Harue Abe Hiroki Miura yoshitaka Motonaga Center for Sustainable Agriculture and Forestry Faculty of AgricultureNiigata University94-2 KodaSadoNiigata952-2206Japan Aomori Prefectural Asamushi Aquarium 1-25 BabayamaAsamushiAomoriAomori039-3501Japan Faculty of Agriculture Niigata University8050 Ikarashi-ninochoNishi-kuNiigata950-2181Japan

In Japan,Camellia japonica and Camellia rusticana are naturally *** differences in their habitats and morphologies,they have been classified by various researchers as either varieties,subspecies,or *** taxonomic position of *** and *** remain unclear because morphological comparisons have been restricted to limited areas and quantitative data are *** grows in snowy places,unlike *** *** displays ornithophily,*** displays *** species have adapted to different growing environments and pollinators,which have altered the morphology of flowers and *** therefore quantitatively estimated the differentiation between these two taxa by comparing the morphologies of leaf hypodermis,flower form,petal color,and filament color in twenty *** findings allowed us to differentiate these two species by the presence or absence of a leaf *** also discovered an intermediate type of leaf hypodermis,which might also be caused by *** component analysis(PCA)indicated that the flower morphologies between these species were significantly *** petal and filament colors were also significantly *** quantitative analysis suggests that speciation caused by differences in both pollinators and environment is one of the factors involved in this *** findings in *** and *** help to explain speciation processes for other species as well.

关键词： Camellia japonica Camellia rusticana Ecological isolation Pollinator shift Pollination syndrome Reproductive isolation

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Present Status of JT-60SA Project and Development of Heating Systems for JT-60SA

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Plasma Science and Technology 2011年第3期13卷 367-375页

作者： yoshitaka IKEDA JT-60SA Team Japan Atomic Energy Agency 801-1 Mukoyama Naka Ibaraki 311-0193 Japan

Present status of the JT-60SA （JT-60 Super Advanced） project, implemented jointly by Europe and Japan since 2007, is described. The design of the main tokamak components was completed in late 2008, and all the scientific missions are preserved to contribute to ITER and DEMO reactors. The construction of the JT-60SA has begun with procurement activities for the superconducting magnet systems, vacuum vessel, in-vessel components and other components under the relevant procurement arrangements between the implementing agencies of JAEA （Japan Atomic Energy Agency） in Japan and Fusion for Energy in Europe. Designs and developments of the auxiliary heating systems for JT-60SA have been progressing at JAEA so as to provide the total injection power of 41 MW for 100 s.

关键词： JT-60SA ITER DEMO JAEA heating systems

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Nonequilibrium molecular dynamics for accelerated computation of ion–ion correlated conductivity beyond Nernst–Einstein limitation

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npj Computational Materials 2023年第1期9卷 1796-1803页

作者： Ryoma Sasaki Bo Gao Taro Hitosugi yoshitaka Tateyama School of Materials and Chemical Technology Tokyo Institute of Technology2-12-1 OokayamaMeguroTokyo 152-8552Japan Center for Green Research on Energy and Environmental Materials National Institute for Material Science1-1 NamikiTsukubaIbaraki 305-0044Japan Department of Chemistry The University of Tokyo7-3-1 HongoBunkyoTokyo 113-0033Japan

Condensed matters with high ionic conductivities are crucial in various solid devices such as solid-state batteries. The conduction ischaracterized by the cooperative ionic motion associated with the high carrier density. However, the high cost of computingcorrelated ionic conductivities has forced almost all ab initio molecular dynamics (MD) to rely on the Nernst–Einstein dilute-solutionapproximation, which ignores the cross-correlation effect. Here we develop a chemical color-diffusion nonequilibrium MD (CCD-NEMD) method, which enables to calculate the correlated conductivities with fewer sampling steps than the conventional MD. ThisCCD-NEMD is demonstrated to well evaluate the conductivities in the representative solid electrolyte bulk Li10GeP2S12 andLi_(7)La_(3)Zr_(2)O_(12). We also applied CCD-NEMD to the grain boundary of Li_(7)La_(3)Zr_(2)O_(12) and demonstrated its applicability for calculatinginterfacial local conductivities, which is essential for investigating grain boundaries and composite electrolytes. CCD-NEMD canprovide further accurate understanding of ionics with ionic correlations and promote developing solid devices.

关键词： dynamics Nernst steps

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Influence of Patch Side of Heat-Ray Absorbing Film on One-Dimensional Unsteady Thermal Stresses in Window Glass

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Transactions of Nanjing University of Aeronautics and Astronautics 2014年第2期31卷 164-168页

作者： yoshitaka Iyama Yoshihiro Obata Department of Mechanical and Aerospace Engineering Graduate School of EngineeringTottori University

Heat-ray absorbing film is used to be bonded on the existing sheet glasses of the *** is effective for air-conditioning energy saving against the global warming,because it absorbs heat-ray in the thin film and decreases the incoming heat-ray into the *** the other hand,the sheet glasses increase the temperature at the surface which the sheet is bonded and sometimes yield heat cracks by thermal *** is important to know the state of thermal stresses accurately in order to develop the heat-ray absorbing film with higher performance and without heat *** this paper,the analysis model is treated as the two-layer plate of the conventional soda sheet glass and the heat-ray absorbing film with different *** unsteady temperature and thermal stresses are analyzed and calculated *** influence of the patch side,which the heat-ray absorbing film is bonded at the exterior side or the interior side,on the heat-ray absorbing performance and the thermal stresses is *** is found that the alternative patch side has no effect on the heat-ray absorbing performance and that the patch side is recommended to be interior side from a view point of decreasing thermal stresses against the heat crack of glasses.

关键词： thermal stress heat crack sheet glass heat-ray absorbing film global warming

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