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Effect of herbal medicine Juzentaihoto on hepatic and intestinal heat shock gene expression requires intestinal microflora in mouse

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World Journal of Gastroenterology 2007年第16期13卷 2289-2297页

作者： Miho Kato Atsushi Ishige Naoko Anjiki Masahiro Yamamoto Yoshifumi Irie Mitsue Taniyama Ryoko Kibe Junichiro Oka yoshimi benno Kenji Watanabe Department of Kampo Medicine Keio University School of Medicine 35 Shinano-machi Shinjuku-ku Tokyo 160-8582 Japan Faculty of Pharmaceutical Science Tokyo University of Science 264 Yamazaki Noda City Chiba 278-8519 Japan Japan Collection of Microorganisms Riken 2-1 Hirosawa Wako-shi Saitama 351-0198 Japan Department of Kampo Medicine Keio University School of Medicine 35 Shinano-machi Shinjuku-ku Tokyo 160-8582 Japan Department of Kampo Medicine Keio University School of Medicine 35 Shinano-machi Shinjuku-ku Tokyo 160-8582 Japan Pharmacology Research Department Tsumura Research Institute Research Division 3586 Yoshiwara Ami-machi Inashiki-gun Ibaraki 300-1192 Japan Pharmacology Research Department Tsumura Research Institute Research Division 3586 Yoshiwara Ami-machi Inashiki-gun Ibaraki 300-1192 Japan Japan Collection of Microorganisms Riken 2-1 Hirosawa Wako-shi Saitama 351-0198 Japan Faculty of Pharmaceutical Science Tokyo University of Science 264 Yamazaki Noda City Chiba 278-8519 Japan

AIM： To evaluate the role of intestinal microflora in the effects of multi-herbal medicine on gene expression in the gut and liver. METHODS： The multi-herbal medicine Juzentaihoto （JTX） was administered to five germ-free mice and regular mice for 2 wk. Among the results of the comprehensive gene chip analysis of the intestine and liver, we featured heat shock proteins （HSPs） 70 and 105 because their gene expression changed only in the presence of microflora. Real-time RT-PCR was performed to confirm the expression levels of these HSP genes. To determine whether JTX acts directly on the HSP genes, sodium arsenite （SA） was used to induce the heat shock proteins directly. To examine the change of the intestinal microflora with administration of JTX, the terminal restriction fragment polymorphism （T-RFLP） method was used. To identify the changed bacteria, DNA sequencing was *** by gene chip and real-time RT-PCR, changed with the administration of JTX in the regular mice but not in the germ-free mice. JTX did not suppress the direct induction of the HSPs by SA. T-RFLP suggested that JTX decreased unculturable bacteria and increased Lactobacillus johnsoni. These data suggested that JTX changed the intestinal microflora which, in turn, changed HSP gene ***： Intestinal microflora affects multi-herbal product JTX on the gene expression in the gut and liver.

关键词： Herbal medicine Juzentaihoto Intestinal microflora Terminal restriction fragment length polymorphism Heat shock protein

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Effects of green tea consumption on human fecal microbiota with special reference to Bifidobacterium species

Effects of green tea consumption on human fecal microbiota w...

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第十四届全国中药和天然药物学术研讨会

作者： Jong-Sik Jin yoshimi benno Chonbuk National University benno Laboratory RIKENSaitama

＜正＞Green tea is one of the most popular beverages in the *** beneficial health effects and components have been extensively ***,little is known about the influence of green tea consumption on the human intestinal microbiota（HIM）,which plays a crucial role in human *** volunteers who did not usually consume green tea,drank it for 10 days and

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