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Alteration of the Expression Levels of Rab3A Affects the Extent of Transcytosis of HRP-Labeled Marker Proteins in Rat CNS Neurons

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Neuroscience & Medicine 2011年第3期2卷 282-287页

作者： yoshiki takeuchi yoshiki Matsumoto Takanori Miki Katsuhiko Warita Zhi-Yu Wang Kuldip S. Bedi Tomiko Yakura Jun-Qian Liu 不详

It has been hypothesized that Rab3A, a small GTPase, may be closely involved in the process of dense core vesicle exocytosis in various cell types. This possibility was investigated by disrupting the expression levels of Rab3A-mRNA using a small interfering RNA of the Rab3A GTPase (Rab3A-siRNA) and examining the effect of this on transcytosis of wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP). Rab3A-siRNA and WGA-HRP were injected into the right vagus nerves of adult rats which were killed 12, 24 or 48 hours later. In some animals, portions of the brain stem containing the nucleus of solitary tract (NST) were prepared for electron microscopy. In other animals, the nodose ganglion of the vagus nerve was used to determine the levels of expression of Rab3A-mRNA using RT-PCR techniques. It was found that the expression of Rab3A-mRNA was markedly depressed in animals at 12 h after the Rab3A-siRNA injection. In the NST, there was an accumulation of HRP-reaction product (RP), recognized as electron dense lysosomal-like structures, in both axons and terminals in the NST 12 h after injection. Some HRP-RP was found in membrane bound vesicles in close proximity to cell membranes and appeared to be in the process of transcytosis. This neuronal transcytosis of HRP-RP appeared to occur at random locations over the axodendritic membranes. These findings indicate that inhibiting the expression of Rab3A-mRNA using Rab3A-siRNA can modulate the level of transcytosis of proteins across neuronal membranes confirming the potentially important role of this GTPase in the process of transcytosis.

关键词： Rab3A-siRNA WGA-HRP Transcytosis Synapse Vagus Nerve Nucleus of Solitary Tract Vesicle Docking Quantitative Real Time RT-PCR

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Autopsy study of anatomical features of the posterior gastric artery for surgical contribution

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World Journal of Gastroenterology 2006年第33期12卷 5357-5359页

作者： Takehiro Okabayashi Michiya Kobayashi Isao Nishimori Kazuya Yuri Takanori Miki yoshiki takeuchi Saburo Onishi Kazuhiro Hanazaki Keijiro Araki Department of Surgery Kochi Medical School Kohasu-Okocho Nankoku-City Japan Department of Gastroenterology and Hepatology Kochi Medical School Kohasu-Okocho Nankoku-City Japan Department of Anatomy Kochi Medical School Kohasu-Okocho Nankoku-City Japan Department of Anatomy and Neurobiology Kagawa University Ikenobe-Kidagun Japan

AIM： To investigate features of the posterior gastric arten/（PGA） with respect to incidence, location and size by using autopsy subjects. METHODS： Autopsies were performed on 72 cadavers of adults with no history of abdominal operations. The localization of the PGA, the distance between the root of the splenic artery and the origin of the PGA, and the external diameter of the PGA were examined. RESULTS： The PGA was recognized in all patients. In 70 （97.2%） cadavers, the PGA branched from the splenic artery, and one female in this group had two PGAs. In 1 （1.4%） patient, the PGA originated from the root of the celiac trunk and in another （1.4%） patient, the PGA branched from the superior polar artery. Overall, the PGA extended for a length of 5.8-12.2 （mean, 8.4） cm from the root of the splenic artery, and the external diameter of the PGA was 1.2-3.2 （mean, 2） mm. CONCLUSION： The anatomical features of the PGA can be readily observed and characterized by autopsy. This study has provided valuable information on the features of the PGA useful in the planning of surgical treatment.

关键词： Posterior gastric artery Gastric carcinoma Autopsy

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Alteration of the Expression Levels of Rab3A Affects the Extent of Transcytosis of HRP-Labeled Marker Proteins in Rat CNS Neurons

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Neuroscience & Medicine（神经系统科学与医药） 2011年第3期

作者： yoshiki takeuchi yoshiki Matsumoto Takanori Miki Katsuhiko Warita Zhi-Yu Wang Kuldip S. Bedi Tomiko Yakura Jun-Qian Liu

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Relationship between Senior Drivers’ Cognitive and Physical Functions and Urban Driving Behaviors during Behind-the-Wheel Driving Education Sessions with Driving Instructors

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Journal of Transportation Technologies 2017年第2期7卷 148-166页

作者： Takashi Yonekawa Takahiro Tanaka Hirofumi Aoki Misako Yamagishi Yuki Yoshihara Eijiro takeuchi Issey Takahashi yoshiki Ninomiya Hitoshi Kanamori Tatsuya Suzuki Makoto Inagami Institute of Innovation for Future Society Nagoya University Nagoya Japan

Recently, traffic accidents caused by elderly drivers have been increasing. It is thought that the reasons of accidents are functions such as decline of eyesight, cognition and physical strength caused by aging. In order to assist safe driving for elderly drivers, it is necessary to sufficiently understand any possible relationship of various senior drivers’ cognition, physical strength and driving behavior. In this paper relationship of elderly driver’s cognition, physical strength and driving behaviors were analyzed using the result of driving instructions on urban road by driving school instructors.

关键词： Human Engineering Elderly Person Driver Characteristics Driving Instruction

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