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World Journal of Gastroenterology 2006年第15期12卷 2341-2344页

作者： Mehryar Habibi Roudkenar Saeid Bouzari Yoshikazu Kuwahara Amaneh Mohammadi Roushandeh Mana Oloomi Manabu Fukumoto Molecular Biology Unit Pasteur Institute of Iran Pasteur AveTehran 13164Iran Department of Pathology Institute of Development Aging and CancerTohoku UniversitySeiryo-cho 4-1Aoba-wardSendai-cityMiyagi-prefecture980-8575Japan Department of Anatomy Medicine Faculty Medical University of TehranTehran 13164Iran

瞄准：在表示粒细胞巨噬细胞殖民地刺激的房间上调查构造混合蛋白质的选择细胞毒素的效果因素(GM-CSF ) 受体。方法：HepG2 (人的肝细胞瘤) 和 LS174T (冒号癌) 在这研究被使用。熔化基因为 4 h 与 0.02% 阿拉伯糖被导致，表示蛋白质被... 详细信息

瞄准：在表示粒细胞巨噬细胞殖民地刺激的房间上调查构造混合蛋白质的选择细胞毒素的效果因素(GM-CSF ) 受体。方法：HepG2 (人的肝细胞瘤) 和 LS174T (冒号癌) 在这研究被使用。熔化基因为 4 h 与 0.02% 阿拉伯糖被导致，表示蛋白质被西方的弄污检测。在 E.coli 表示的妄想的蛋白质在这些房间上为它的细胞毒素的活动被检查， apoptosis 被彗星试金并且原子染色测量。结果：妄想的蛋白质被发现对表示 GM-CSFRs 的结肠癌房间线，然而并非到缺乏这些受体的 HepG2 细胞毒素。最大的活动在 24 h 孵化以后在 40 ng/mL 的集中被观察。IC (50 ) 是 20+/-3.5 ng/mL。结论：结肠癌房间上的混合蛋白质的选择细胞毒素的效果排队表示 GM-CSF 受体(GM-CSFRs ) 受体和 apoptosis 能在这根房间线被观察。混合蛋白质能被看作一个治疗学的代理人。

关键词：重组体杂交蛋白毒素巨噬细胞结肠癌

Carbon-Ion Beams Efficiently Induce Cell Killing in X-Ray Resistant Human Squamous Tongue Cancer Cells

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International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2014年第3期3卷 133-142页

作者： Akihisa Takahashi Hongyu Ma Akiko Nakagawa Yukari Yoshida Tatsuaki Kanai Tatsuya Ohno Yoshikazu Kuwahara Manabu Fukumoto Takashi Nakano Advanced Scientific Research Leaders Development Unit Gunma University Gunma Japan Department of Pathology IDAC Tohoku University Sendai Japan Department of Radiation Oncology Gunma University Graduate School of Medicine Gunma Japan Gunma University Heavy Ion Medical Center Gunma Japan

In order to see whether carbon ion (C-ion) beams have a biological advantage over X-rays, studies were designed to examine the effects of C-ion beams on radiosensitivity in X-ray resistant cells. Clinically relevant X-ray resistant SAS-R cells derived from human tongue cancer SAS cells were used. The cells were exposed to X-rays or Spread-Out Bragg peak (SOBP) beam C-ions. Cell survival was measured using a modified high-density survival assay. Cell survival signaling and cell death signaling were analyzed using flow cytometry. The cells were labeled with putative cancer stem cell markers such as CD44 and CD326. SAS-R cells were 1.6 times more radioresistant than SAS cells after exposure to X-rays. Cell survival was similar in each cell line after exposure to C-ion beams. SAS-R cells displayed enhanced cell survival signaling when compared to SAS cells under normal conditions. On the other hand, the phosphorylation of AKT-related proteins decreased and polycaspase activities were enhanced when cells were irradiated with C-ion beams in both cell lines. More CD44 and CD326 positive cells were seen in SAS-R cells than in SAS cells. Moreover, the marker positive cell numbers significantly decreased after exposure to C-ion beams when compared to X-rays at iso-survival doses in SAS-R cells. C-ion beams efficiently induced cell killing in X-ray resistant cells which displayed activated cell survival signaling and contained more numerous cancer stem-like cells.

关键词： Carbon-Ion Beams X-Ray Resistant Cells Cell Survival and Death Signaling Cancer Stem-Like Cells

X-Ray Induced Mutation Frequency at the <i>Hypoxanthine Phosphoribosyltransferase</i>Locus in Clinically Relevant Radioresistant Cells

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International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2017年第4期6卷 377-391页

作者： Yoshikazu Kuwahara Mehryar Habibi Roudkenar Yusuke Urushihara Yohei Saito Kazuo Tomita Amaneh Mohammadi Roushandeh Tomoaki Sato Akihiro Kurimasa Manabu Fukumoto Division of Radiation Biology and Medicine Faculty of Medicine Tohoku Medical and Pharmaceutical University Sendai Japan Department of Applied Pharmacology Graduate School of Medical and Dental Sciences Kagoshima University Kagoshima Japan Medical Biotechnology Research Center Paramedicine Faculty Guilan University of Medical Science Rasht Iran Fukushima Project Headquarters National Institute for Quantum and Radiological Science and Technology Fukushima Japan Department of Radiopharmacy Faculty of Pharmaceutical Science Tohoku Medical and Pharmaceutical University Sendai Japan Department of Anatomical Sciences School of Medicine Hamadan University of Medical Sciences Hamadan Iran Department of Molecular Pathology Tokyo Medical University Tokyo Japan

To elucidate the molecular mechanisms underlying cellular radioresistance, clinically relevant radioresistant cell lines were established via long-term exposure to X-rays with stepwise dose escalation. Established cells continue to proliferate despite exposure to 2 Gy X-rays/day for more than 30 days, a standard protocol in cancer radiotherapy. DNA repair fidelity in radioresistant and the parental cells by evaluating the mutation frequency at the hypoxanthine phosphoribosyltransferase (HPRT) locus after exposure to X-rays was determined. Mutation spectrum at the HPRT locus was examined by multiplex polymerase chain reaction. Rejoining kinetics of X-ray-induced DNA double strand breaks (dsbs) was evaluated by the detection of phosphorylated histone H2AX (γH2AX) after X-irradiation. The fold increase in the HPRT mutation frequency due to acute radiation was similar between radioresistant and the parental cell lines. However, fractionated radiation (FR) consisting of 2 Gy X-rays/day increased the mutation frequency at the HPRT locus in parental but not in radioresistant cells. Analysis of the FR-induced mutations at the HPRT locus revealed a high frequency of deletion mutations (>70%) in parental but not in radioresistant cells. As assessed by γH2AX immunostaining, DNA dsbs induced by acute exposure to 10 Gy of X-rays were repaired to the control level within 7 days in radioresistant but not in the parental cells. Moreover, 2 Gy × 5 FR increased the number of γH2AX-positive cells in parental cultures but not in radioresistant cultures. DNA dsbs induced by 2 Gy/day FR are repaired with fidelity in radioresistant but not in parental cells.

关键词： Clinically Relevant Radioresistant (CRR) Cell Hypoxanthine Phosphoribosyltransferase (HPRT) Mutation Frequency Phosphorylated Histone H2AX (γH2AX) X-Rays

Mass screening-based case-control study of diet and prostate cancer in Changchun, China

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Asian Journal of Andrology 2008年第4期10卷 551-560页

作者： Xiao-Meng Li Jiang Li Ichiro Tsuji Naoki Nakaya yoshikazu Nishino Xue-Jian Zhao School of Life Sciences Northeast Normal University Changchun 130024 China School of Basic Medical Sciences Changchun 130041 China Dental Hospital Jilin University Changchun 130041 China Department of Public Health Tohoku University Sendai 980-8576 Japan

Aim： To investigate possible correlation factors for prostate cancer by a population-based case-control study in China. Methods： We carded out a mass screening of prostate cancer in Changchun, China, using a prostate-specific antigen assisted by Japan International Cooperation Agency. From June 1998 to December 2000, 3 940 men over 50 years old were screened. Of these, 29 men were diagnosed with prostate cancer. We selected 28 cases and matched them with controls of low prostate-specific antigen value （〈 4.1 ng/mL） by 1：10 according to age and place of employment. A case-control study of diet and prostate cancer was then carded out. Results： After adjustment for education, body mass index （BMI）, smoking, alcohol consumption, marriage and diet, intake of soybean product was discovered to be inversely related to prostate cancer. Men who consumed soybean product more than twice per week on different days had a multivariate odds ratio （OR） of 0.38 （95% confidence interval [CI], 0.13-1.12）. In addition, men who consumed soybean products more than once per day had a multivariate OR of 0.29 （95% CI, 0.11-0.79） compared with men who consumed soybean products less than once per week. The P for trend was 0.02, which showed significant difference. There was no significant difference in P trend for any dairy food. Even when we matched the cases and controls by other criteria, we found that soybean food was the only preventive factor associated with prostate cancer. Conclusion： Our study suggests that consumption of soybeans, one of the most popular foods in Asia, would decrease the risk of prostate cancer.

关键词： soybean prostate cancer case-control study diet