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Phosphorylation Properties of the N-Terminal Region of Twitchin from Molluscan Catch Muscle

American Journal of Molecular Biology 2019年第3期9卷 110-120页

作者： Daisuke Funabara Yuuki nishimura Satoshi Kanoh Graduate School of Bioresources Mie University Tsu Japan

Molluscan smooth muscles, such as the bivalve adductor muscles and the mussel anterior byssus retractor muscles (ABRM), exhibit a unique contraction called “catch”. Catch contraction is regulated through twitchin phosphorylation and dephosphorylation. Twitchin from the ABRM of the Mediterranean mussel, Mytilus galloprovincialis, is phosphorylated by cAMP-dependent protein kinase (PKA), and PKA phosphorylation sites are located in both the N- and C-terminal regions of the twitchin molecule. The D2 site, which is adjacently located to the C-terminus, participates in forming a myosin, actin, and twitchin complex that is thought to contribute towards the maintenance of tension in the catch state. In contrast, although it has been reported to interact with thin-filaments, the molecular function of the region including the D1 site has remained largely unstudied. Three additional PKA consensus sequences were identified near the D1 site;however, it was not known if these sites could be directly phosphorylated by PKA. Here, we performed phosphorylation assays to identify phosphorylation sites near the D1 site using recombinant protein variants (TWD1-SSSS, TWD1-AAAS, TWD1-AASA, TWD1-ASAA, TWD1-SAAA, and TWD1-AAAA). All variants, except TWD1-AAAA (where all phosphorylatable serine residues were replaced by alanines), were phosphorylated by PKA. The four phosphorylation sites were named D1-1, D1-2, D1-3, and D1-4 (the originally identified D1) in order from the N-terminus. Phosphorylation assays using a 1/12.5 weight ratio of PKA to each TWD1 variant revealed that D1-4 was the most rapidly phosphorylated, closely followed by D1-1. However, D1-2 and D1-3 were phosphorylated at a lower level under equivalent conditions and were not phosphorylated when PKA was incubated with each TWD1 variant at a 1/100 weight ratio. Furthermore, we observed that TWD1-SSSS was phosphorylated in a stepwise fashion. These findings contribute towards the elucidation of the function of the twitchin D1

关键词： Catch Contraction Phosphorylation Phos-Tag Gel Electrophoresis PKA Twitchin

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Sediment Oxygen Demand of a Leachate Pond at an Offshore Municipal Solid Waste Disposal Site 2 Years after the Site Was Closed

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Journal of Geoscience and Environment Protection 2018年第4期6卷 181-193页

作者： Shogo Sakita Jun Nishimoto Kazuyuki nishimura Department of Environmental Sciences Prefectural University of Hiroshima Shobara City Japan

Various water quality parameters of a leachate pond at an offshore municipal solid waste disposal site were monitored. The pH, dissolved oxygen (DO), and water temperature at the bottom of the leachate pond were measured during Sep. (the 1st period) and Nov.-Dec. (the 2nd period) of 2011. The results suggested that the stratification of water temperature in the pond had gradually broken down due to convection occurring between the end of the 1st period and the 2nd period. The pH was almost constant at 10 - 11 during the 1st period and was approximately 11.5 during the 2nd period. The DO was almost zero during both periods. An anaerobic batch experiment with sampled sediment was undertaken to elucidate the mechanism of material leaching from the sediment. DO decreased under all experimental conditions. With respect to oxidation reduction potential (ORP) and total sulfide in addition to DO, the condition most closely mimicking that of the site became the most anaerobic. The average sediment oxygen demand, SODave, was calculated using a brief numerical model based on batch experiment data. The SODave was 1114.7 mg/m2/d, indicating that at least 434 g/d of oxygen must be supplied to the leachate pond to maintain the DO.

关键词： Offshore Disposal Site Leachate Pond Sediment Sediment Oxygen Demand

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Role of narrow band imaging in endoscopic submucosal dissection

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World Journal of Gastrointestinal Endoscopy 2012年第9期4卷 387-397页

作者： Kouichi Nonaka Makoto nishimura Hiroto Kita Department of Gastroenterology Saitama Medical University International Medical CenterSaitama 350-1298Japan

Narrow band imaging(NBI) is a new image enhancement system employing optic digital methods to enhance images of blood vessels on mucosal surfaces,allowing improved visualization of mucosal surface *** have progressed over the last several years,and the clinical usefulness has been *** has become frequently applied for preoperative diagnosis before endoscopic submucosal dissection(ESD) of digestive tract cancers,as well as for assessment of the range of ESD for en-bloc resection of large *** has been reached with regard to the usefulness of NBI for detecting micro-lesions of esophageal squamous cell carcinoma indicated for ESD,for the diagnosis of the range and *** has also been attracting attention for diagnosing gastric cancer based on the observation of micro blood vessels on the mucosal surface and mucosal surface *** usefulness of NBI has been reported in relation to various aspects of colon cancer,including diagnoses of the presence,quality,range,and depth of ***,as NBI has not surpassed diagnostic methods based on magnifying observation combined with the established and widely employed dye method,its role in ESD is limited at *** NBI is very useful for the diagnosis of digestive tract cancers,comprehensive endoscopic diagnosis employing the combination of conventional endoscopy including dye spraying,EUS,and NBI may be important and essential for ESD.

关键词： Endoscopic submucosal dissection Narrow band imaging Digestive tract cancer

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Corrosion Performance of Si and Al-bearing Ultrafine Grained Weathering Steel

Corrosion Performance of Si and Al-bearing Ultrafine Grained...

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第二届先进钢铁结构材料国际会议

作者： T.nishimura Corrosion Resistant Group Steel Research Center National Institute for Material Science （NIMS） Japan

＜正＞The iron rust phase has been analyzed by using EPMA and TEM after simulating marine corrosion tests. The ultrafine grained （UFG） weathering steel containing Si and Al showed higher corrosion resistance than carbon steel in the *** and Si were identified as A13+ and Si2+ in the complex oxide of inner rust by EPMA and TEM. It was demonstrated that that Si and Al take part in the conversion of inner rust into fine structure that prevents the penetration of Cl ions.

关键词： Rust Al Si Wethering Steel Ultrafine Grain

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肿瘤的细胞免疫治疗

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郑州大学学报（医学版） 2011年第2期46卷 165-169页

作者：张毅 Michael nishimura 郑州大学第一附属医院生物细胞治疗中心郑州450052 郑州大学生物工程系郑州450001 美国南卡罗来纳州医科大学外科学查理斯顿29425

近年来,肿瘤的常规治疗方法均取得了很大改进,患者的长期存活率有所提高。手术、放射治疗和化学治疗能治愈部分肿瘤患者,但由于不能杀死全部肿瘤细胞,多数患者仍不能免于复发或转移的发生。作为现代肿瘤治疗的第4种模式,

关键词：肿瘤细胞免疫治疗肿瘤抗原肿瘤浸润性淋巴细胞

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7β-[1-(2-氨噻唑基-4)-1-环丙甲酰氨]头孢烯衍生物的合成及抗菌活性

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国外医药（抗生素分册） 1992年第3期13卷 169-171页

作者： nishimura S 严敏

自发现头孢唑肟、头孢甲肟及头孢噻肟具有强的抗菌活性以来,众多研究工作者对合成烷氧亚氨头孢烯衍生物的研究产生了极大兴趣。在头孢烯C-7位上引入烷氧亚氨基能导致抗菌活性增强并扩大抗菌谱。另一方面,新型的头孢烯衍生物,如SG-164(3)... 详细信息

自发现头孢唑肟、头孢甲肟及头孢噻肟具有强的抗菌活性以来,众多研究工作者对合成烷氧亚氨头孢烯衍生物的研究产生了极大兴趣。在头孢烯C-7位上引入烷氧亚氨基能导致抗菌活性增强并扩大抗菌谱。另一方面,新型的头孢烯衍生物,如SG-164(3)与7432-S(4)。

关键词：头孢烯衍生物合成抗菌活性

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Outcomes of endoscopic endonasal dacryocysto-rhinostomy for intractable lacrimal dacryostenosis and associated factors

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International Journal of Ophthalmology(English edition) 2016年第10期9卷 1471-1475页

作者： Tohru Tanigawa Hirokazu Sasaki Hiroshi Nonoyama Yuichiro Horibe Kunihiro nishimura Tetsuro Hoshino Tetsuya Ogawa Kenta Murotani Hiromi Ueda Masahiro Kaneda Department of Otolaryngology Aichi Medical University Department of Otolaryngology Tono Kosei Hospital Division of Biostatistics Clinical Research CenterAichi Medical University Department of Ophthalmology Tono Kosei Hospital

AIM：To examine the effects of patient age,canalicular obstruction,mode of anesthesia,and duration of nasolacrimal intubation on the outcomes of endoscopic endonasal dacryocystorhinostomy（DCR）. METHODS： Totally 56 eyes of 46 patients with prolonged epiphora underwent minimally invasive endoscopic endonasal DCR.A successful surgical outcome was defined as a significant improvement in symptoms,adequate water passage from the puncta to the nasal cavity,and patency of the DCR *** outcomes were assessed at least 6mo after ***＇s exact test was used to discuss the factors,and then the logistic regression analysis was made by SAS9.4 ***： The overall success rate was 75.0%,and complete resolution was observed in 27 *** success rate was higher for patients with ≥6mo intubation than for those with 〈6mo ***,there were no significant differences in outcomes between groups stratified by age（〈65 or ≥65y）,presence or absence of canalicular obstruction,mode of anesthesia（local or general）,and use or nonuse of a radiowave *** patient developed subcutaneous emphysema around the eye and nose and one developed subcutaneous hemorrhage after surgery. CONCLUSION： Endoscopic endonasal DCR can be considered safe and minimally invasive with reasonable success rates,particularly when the duration of nasolacrimal intubation is ≥6mo.

关键词： dacryocystorhinostomy endoscopy,, intubation epiphora

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Unprecedented differences in the diamond nucleation density between carbon-and silicon-faces of 4H-silicon carbides

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Chinese Chemical Letters 2020年第7期31卷 2013-2018页

作者： Bo Wang Pitsiri Sukkaew Guichen Song Andreas Rosenkranz Yunxiang Lu Kazhihito nishimura Jia Wang Jilei Lyu Yang Cao Jian Yi Lars Ojamae He Li Nanjiang Key Laboratory of Marine New Materials and Related Technology Zhejiang Key Laboratory of Marine Materials and Protection TechnologyNingbo Institute of Material Technology&EngineeringChinese Academy of SciencesNingbo 315201China Department of Physics Chemistryand BiologyLinkoping UniversitySE-58183 LinkopingSweden Department of Chemical Engineering Biotechnology and MaterialsUniversidad de ChileAvenida Beauchef 851SantiagoChile Mechanical Systems Engineering Kogakuin UniversityTokyo 192-0015Japan Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE 68588United State

4H-silicon carbides deposited by diamond films have wide applications in many fields such as semiconductor heterojunction,heat sink and mechanical *** plays a critical role in the deposition of the diamond film on 4H-silicon ***,as a typical polar material,the fundamental mechanism of diamond nucleation on different faces of 4H-silicon carbides has not been fully understood *** this contribution,nucleation of diamond was performed on the carbon-and silicon-faces of 4H-silicon carbides in a direct current chemical vapor deposition *** nucleation density on the carbon-face is higher by 2-3 orders of magnitude compared to the *** electron microscopy verifies that there are high density diamond nuclei on the interface between the carbon-face and the diamond film,which is different from columnar diamond growth structure on the *** state theory calculation reveals that the unprecedented distinction of the nucleation density between the carbon-face and the silicon-face is attributed to different desorption rates of the absorbed hydrocarbon *** addition,kinetic model simulations demonstrate that it is more difficult to form CH2(s)-CH2(s)dimers on silicon-faces than carbon-faces,resulting in much lower nucleation densities on silicon-faces.

关键词： 4H-silicon carbide Diamond nucleation mechanism Transmission electron microscopy Transition state theory Kinetic model simulation

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时间轴上的徘徊——北京西城区城市更新探索

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世界建筑 2004年第2期 58-64页

作者： Riki nishimura,Rachel Jaffe MAUD MUP

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Preoperative detection and localization of small bowel hemangioma: Two case reports

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World Journal of Gastroenterology 2017年第20期23卷 3752-3757页

作者： Nobuhisa Takase Keisuke Fukui Takafumi Tani Tohru nishimura Tomohiro Tanaka Naoki Harada Kimihiko Ueno Manabu Takamatsu Akihiko Nishizawa Akiharu Okamura Kunihiko Kaneda Department of Surgery Kakogawa Central City Hospital Department of Internal Medicine Kakogawa Central City Hospital Department of Diagnostic Pathology Kakogawa Central City Hospital

Among the various diagnostic modalities for small bowel hemangioma, video capsule endoscopy (VCE) and double-balloon enteroscopy (DBE) can be recommended as part of the work-up in patients with obscure gastrointestinal bleeding (OGIB). DBE is superior to VCE in the accuracy of diagnosis and therapeutic potential, while in most cases total enteroscopy cannot be achieved through only the antegrade or retrograde DBE procedures. As treatment for small bowel bleeding, especially spout bleeding, localization of the lesion for the decision of DBE insertion facilitates early treatment, such as endoscopic hemostatic clipping, allowing patients to avoid useless transfusion and the worsening of their disease into life-threatening status. Applying endoscopic India ink marking prior to laparoscopic surgical resection is a particularly useful technique for more minimally invasive treatment. We report two cases of small bowel hemangioma found in examinations for OGIB that were treated with combination of laparoscopic and endoscopic modalities.

关键词： Laparoscopic surgery India ink marking Small bowel hemangioma Obscure gastrointestinal bleeding Minimally invasive

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