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Glucagon-like peptide-1 analogue prevents nonalcoholic steatohepatitis in non-obese mice

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World Journal of Gastroenterology 2016年第8期22卷 2512-2523页

作者： Takaya Yamamoto Yukiomi Nakade Taeko yamauchi Yuji Kobayashi Norimitsu Ishii Tomohiko Ohashi Kiyoaki Ito Ken Sato Yoshitaka Fukuzawa Masashi Yoneda Division of Hepatology and Pancreatology Department of Internal Medicine Aichi Medical University

AIM: To investigate whether a glucagon-like peptide-1(GLP-1) analogue inhibits nonalcoholic steatohepatitis(NASH), which is being increasingly recognized in Asia, in non-obese mice. METHODS: A methionine-choline-deficient diet(MCD) along with exendin-4(20 μg/kg per day, ip), a GLP-1 analogue, or saline was administered to male db/db mice(non-obese NASH model). Four or eight weeks after commencement of the diet, the mice were sacrificed and their livers were excised. The excised livers were examined by histochemistry for evidence of hepatic steatosis and inflammation. Hepatic triglyceride(TG) and free fatty acid(FFA) content was measured, and the expression of hepatic fat metabolism- and inflammation-related genes was evaluated. Oxidative stress-related parameters and macrophage recruitment were also examined using ***: Four weeks of MCD feeding induced hepatic steatosis and inflammation and increased the hepatic TG and FFA content. The expression of fattyacid transport protein 4(FATP4), a hepatic FFA influxrelated gene; macrophage recruitment; and the level of malondialdehyde(MDA), an oxidative stress marker, were significantly augmented by a 4-wk MCD. The levels of hepatic sterol regulatory element-binding protein-1c(SREBP-1c) m RNA(lipogenesis-related gene) and acyl-coenzyme A oxidase 1(ACOX1) m RNA(β-oxidation-related gene) had decreased at 4 wk and further decreased at 8 wk. However, the level of microsomal triglyceride transfer protein m RNA(a lipid excretion-related gene) remained unchanged. The administration of exendin-4 significantly attenuated the MCD-induced increase in hepatic steatosis, hepatic TG and FFA content, and FATP4 expression as well as the MCD-induced augmentation of hepatic inflammation, macrophage recruitment, and MDA levels. Additionally, it further decreased the hepatic SREBP-1c level and alleviated the MCD-mediated inhibition of the ACOX1 m RNA level. CONCLUSION: These results suggest that GLP-1 inhibits hepatic s

关键词： Glucagon like peptide-1 Nonalcoholic steatohepatitis Kupffer cells Free fatty acid Oxidative stress

Short-type single balloon enteroscope for endoscopic retrograde cholangiopancreatography with altered gastrointestinal anatomy

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World Journal of Gastroenterology 2013年第11期19卷 1728-1735页

作者： Hiroshi yamauchi Mitsuhiro Kida Kosuke Okuwaki Shiro Miyazawa Tomohisa Iwai Miyoko Takezawa Hidehiko Kikuchi Maya Watanabe Hiroshi Imaizumi Wasaburo Koizumi Department of Gastroenterology Kitasato University East HospitalSagamiharaKanagawa 252-0380Japan

AIM:To evaluate the effectiveness of a short-type single-balloon-enteroscope(SBE) for endoscopic retrograde cholangiopancreatography(ERCP) in patients with a reconstructed ***:Short-type SBE was developed to perform ERCP in postoperative patients with a reconstructed ***-type SBE is a direct-viewing endoscope with the following specifications:working length,1520 mm;total length,1840 mm;channel diameter,3.2 *** addition,short-type SBE has a water-jet *** study group comprised 22 patients who underwent 31 sessions of short-type SBE-assisted ERCP from June 2011 through May *** was performed by Billroth-Ⅱ(B-Ⅱ) gastrectomy in 6 patients(8 sessions),Roux-en-Y(R-Y) gastrectomy in 14 patients(21 sessions),and R-Y hepaticojejunostomy in 2 patients(2 sessions).We retrospectively studied the rate of reaching the blind end(papilla of Vater or choledochojejunal anastomosis),mean time required to reach the blind end,diagnostic success rate(defined as the rate of successfully imaging the bile and pancreatic ducts),therapeutic success rate(defined as the rate of successfully completing endoscopic treatment),mean procedure time,and ***:Among the 31 sessions of ERCP,the rate of reaching the blind end was 88% in B-Ⅱ gastrectomy,91% in R-Y gastrectomy,and 100% in R-Y *** mean time required to reach the papilla was 18.3 min in B-Ⅱ gastrectomy,21.1 min in R-Y gastrectomy,and 32.5 min in R-Y *** diagnostic success rates in all patients and those with an intact papilla were respectively 86% and 86% in B-Ⅱ gastrectomy,90% and 87% in R-Y gastrectomy,and 100% in R-Y *** therapeutic success rates in all patients and those with an intact papilla were respectively 100% and 100% in B-Ⅱ gastrectomy,94% and 92% in R-Y gastrectomy,and 100% in R-Y *** the channel diameter was 3.2 mm,stone extraction could be performed with a wire-guided basket in 12

关键词： Endoscopic retrograde cholangiopancreatography Single-balloon-enteroscope Short type BillrothII gastrectomy Roux-en-Y gastrectomy

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发动机稀薄燃烧控制系统

小型内燃机 1990年第5期19卷 54-59页

作者： T.yamauchi T.Nogi 任雄峰

本文介绍一种新型发动机稀薄燃烧控制系统。采用燃油雾化器,可获得直径为40μm精细油滴的均匀混合气。为在气缸内空燃比8～26范围内实现精确而快速的响应控制,研制了一种新型空燃比传感器,并研究出一种空气流量的综合控制方法。在常速... 详细信息

本文介绍一种新型发动机稀薄燃烧控制系统。采用燃油雾化器,可获得直径为40μm精细油滴的均匀混合气。为在气缸内空燃比8～26范围内实现精确而快速的响应控制,研制了一种新型空燃比传感器,并研究出一种空气流量的综合控制方法。在常速行车条件下,把平均空燃比加大到25,可显著地改进油耗和排放。这是由精细混合气稳定燃烧而实现的。这个系统既可确保司机获得平稳的驾驶性能,又可实现快速的车辆响应和良好的燃油经济性。

关键词：发动机燃烧控制系统汽车

EVALUATION BONE MASS OF RATS BY DUAL ENERGY X-RAY ABSORPTIOMETRY:INFLUENCE OF BODY WEIGHT AND NASOANAL LENGTH

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EVALUATION BONE MASS OF RATS BY DUAL ENERGY X-RAY ABSORPTIOM...

Usefulness of serum lipase for early diagnosis of post-endoscopic retrograde cholangiopancreatography pancreatitis

第二届国际骨质疏松研讨会

作者： X.Li K.Kushida K.Yamazaki H.yamauchi M.Takahashi T.Inoue The second Hospital of Harbin Medical University Department of Orthopedic Surgery Hamamatsu University School of Medicine

＜正＞INTRODUCTION The effects of body weight,body mass index（BMI） and other factors on bone mineral density（BMD） and bone mineral content（BMC） measured by dual energy x-ray absorptiometry（DXA） have been studied in ***,the effects of body weight and naso-anal length on bone mass in rats were less published,therefore we conduct this study.

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World Journal of Gastrointestinal Endoscopy 2019年第9期11卷 477-485页

作者： Masayoshi Tadehara Kosuke Okuwaki Hiroshi Imaizumi Mitsuhiro Kida Tomohisa Iwai Hiroshi yamauchi Toru Kaneko Rikiya Hasegawa Eiji Miyata Yusuke Kawaguchi Hironori Masutani Wasaburo Koizumi Department of Gastroenterology Kitasato University School of Medicine

BACKGROUND Post-endoscopic retrograde cholangiopancreatography(ERCP)pancreatitis(PEP)is new onset acute pancreatitis after *** complication is sometimes *** such,PEP should be diagnosed early so that therapeutic interventions can be carried *** lipase(s-Lip)is useful for diagnosing acute ***,its usefulness for diagnosing PEP has not been sufficiently *** This study aimed to retrospectively examine the usefulness of s-Lip for the early diagnosis of *** We retrospectively examined 4192 patients who underwent ERCP at our two hospitals over the last 5 *** primary outcomes were a comparison of the areas under the receiver operating characteristic(ROC)curves(AUCs)of s-Lip and serum amylase(s-Amy),s-Lip and s-Amy cutoff values based on the presence or absence of PEP in the early stage after ERCP via ROC curves,and the diagnostic properties[sensitivities,specificities,positive predictive values(PPV),and negative predictive value(NPV)]of these cutoff values for PEP *** Based on the eligibility and exclusion criteria,804 cases were *** the entire course,PEP occurred in 78 patients(9.7%).It occurred in the early stage after ERCP in 40 patients(51.3%)and in the late stage after ERCP in 38 patients(48.7%).The AUCs were 0.908 for s-Lip[95%confidence interval(CI):0.880-0.940,P<0.001]and 0.880 for s-Amy(95%CI:0.846-0.915,P<0.001),indicating both are useful for early *** comparing the AUCs,s-Lip was found to be significantly more useful for the early diagnosis of PEP than s-Amy(P=0.023).The optimal cutoff values calculated from the ROC curves were 342 U/L for s-Lip(sensitivity,0.859;specificity,0.867;PPV,0.405;NPV,0.981)and 171 U/L for s-Amy(sensitivity,0.859;specificity,0.763;PPV,0.277;NPV,0.979).CONCLUSION S-Lip was significantly more useful for the early diagnosis of *** s-Lip after ERCP could help diagnose PEP earlier;hence,therapeutic interventions can be provided earlier.

关键词： Endoscopic retrograde cholangiopancreatography Post-endoscopic retrograde cholangiopancreatography pancreatitis Pancreatitis Lipase Amylase

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彩色摄象机实时重合校正系统的开发

电视技术 1986年第5期 45-46页

作者： Kazuki Iwabe Kunio yamauchi Fumio Ukigaya 刘保华

本文介绍三管式彩色摄象机实时重合校正系统的开发。迄今为止,动态重合误差,在Ⅰ区超过0.1％,在Ⅲ区的重合误差超过0.5％,从而损害了图象质量。采用该系统后,Ⅰ区的动态重合误差在0.05％以内,Ⅲ区为0.1％。为了实时工作,采用“曲面近似... 详细信息

本文介绍三管式彩色摄象机实时重合校正系统的开发。迄今为止,动态重合误差,在Ⅰ区超过0.1％,在Ⅲ区的重合误差超过0.5％,从而损害了图象质量。采用该系统后,Ⅰ区的动态重合误差在0.05％以内,Ⅲ区为0.1％。为了实时工作,采用“曲面近似法”(curved surface approximation)。在十毫秒内计算出校正信号,并每隔10.05秒从摄象机内的微处理机送到扫描偏转电路进行校正。

关键词：摄象机偏转电路微处理机传感器重合误差横向色差校正系统图象质量校正信号

母鼠有机磷农药暴露对着床前期小鼠胚胎的细胞遗传学及生长发育的影响(英文)

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环境与职业医学 2004年第5期21卷 391-393页

作者：田英石川仁山内彻上海第二医科大学公共卫生学院预防医学教研室上海200025 日本三重大学医学部公共卫生教研室

[目的 ]研究妊娠初期有机磷农药暴露对母体生殖功能 ,对子代细胞遗传学及生长发育的影响。 [方法 ]以有机磷农药敌百虫为受试物 ,以着床期胚胎为研究对象 ,以反映染色体异常的微核形成和反映胚胎生长发育状况的构成细胞数为指标。孕鼠... 详细信息

[目的 ]研究妊娠初期有机磷农药暴露对母体生殖功能 ,对子代细胞遗传学及生长发育的影响。 [方法 ]以有机磷农药敌百虫为受试物 ,以着床期胚胎为研究对象 ,以反映染色体异常的微核形成和反映胚胎生长发育状况的构成细胞数为指标。孕鼠腹腔注射 10 0或 2 0 0mg/kg剂量的敌百虫溶液 ,3d后 ,在体视显微镜下从其子宫中取出胚胎 ,进行其形态学分析 ,微核试验及胚胎构成细胞数的分析。 [结果 ]敌百虫暴露组的胚胎微核发生率明显高于对照组 ,分别为 2 0 0mg/kg暴露组为16 9% ,10 0mg/kg暴露组 16.1% ,对照组为 5 .3 % ,并有明显的剂量反应关系。此外 ,母鼠暴露于敌百虫 ,可造成子代生长发育迟缓 ,表现为胚胎构成细胞数明显下降 ( 2 0 0mg/kg组 44 .1,对照组 5 4.7)。 [结论 ]妊娠初期有机磷农药暴露对着床前期胚胎的细胞遗传学及生长发育有明显影响。着床前期小鼠胚胎的微核试验是评价母体早期暴露有机磷农药导致子代细胞遗传学改变的简便、灵敏的指标 ,本结果为进一步对着床后胚胎的研究。

关键词：暴露胚胎着床前细胞遗传学有机磷农药对照组细胞数生长发育敌百虫母鼠

Pyrin重组蛋白对支气管哮喘小鼠气道炎症的影响

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中华微生物学和免疫学杂志 2012年第2期32卷 102-107页

作者：延光海朴红梅安昌善山内广平延边大学医学部解剖学教研部延吉133002 延边大学附属医院呼吸内科延吉133002 日本岩手医科大学

目的探讨Pydn重组蛋白对支气管哮喘小鼠气道炎症的抑制作用及机制的研究。方法40只雄性清洁级BALB／c小鼠，随机分为4组，每组10只。分别为生理盐水对照组、卵蛋白（OVA）哮喘组、Pyrin重组蛋白治疗3d组和Pyrin重组蛋白治疗7d组。在末... 详细信息

目的探讨Pydn重组蛋白对支气管哮喘小鼠气道炎症的抑制作用及机制的研究。方法40只雄性清洁级BALB／c小鼠，随机分为4组，每组10只。分别为生理盐水对照组、卵蛋白（OVA）哮喘组、Pyrin重组蛋白治疗3d组和Pyrin重组蛋白治疗7d组。在末次激发24h后所有小鼠取左肺组织行苏木精．伊红（HE）染色，Masson三色染色；用图像分析软件测定支气管壁厚度（WAt／Pbm）、支气管平滑肌厚度（WAm／Pbm）及胶原纤维面积；用酶联免疫吸附法（ELISA）检测支气管肺泡灌洗液（bronchoalveolarlavage：fluid，BALF）中IL-4、IL-5、TNF—α及IFN-1含量；用RT—PCR检测肺组织中结缔组织生长因子（connectivetissuegrowthfactor，CTGF）和转化生长因子-β1（transforminggrowthfactor-61，TGF—β1）的mRNA表达；用Westernblot方法检测核转录因子（nuclearfactorkappaB，NF-KB）的表达。结果哮喘模型组小鼠BALF中细胞总数、IL4、IL-5、TNF-仪水平增高和IFN-1水平降低；肺组织支气管管壁厚度、气道平滑肌厚度、胶原纤维面积，CTGF、TGF-β1的转录和表达水平以及NF—κB表达水平均显著高于生理盐水对照组（P〈O．05）。Pyfin重组蛋白3d和7d干预组小鼠BALF中细胞总数、IL-4、IL-5、TNF-α水平和肺组织支气管管壁厚度、气道平滑肌厚度、胶原纤维面积、NF—κB表达水平显著降低，与哮喘模型组比较差异均具有统计学意义（P〈0．05），而BALF中IFN-γ水平明显上升，与哮喘模型组比较，差异均具有统计学意义（P〈O．05）；P州n重组蛋白7d干预组CTGF、TGF-β1的转录和表达水平显著低于哮喘组（P〈0．05）。结论我们推测Pyrin重组蛋白可能部分是通过抑制NF-κB信号途径来阻断TGF-β1和CTGF的过度表达而实现抑制气道炎症，进而抑制气道重构的发生。

关键词：支气管哮喘气道重构小鼠 Pyrin重组蛋白

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东北某城市大气颗粒污染物中有毒重金属浓度

大连医科大学学报 2007年第4期29卷 332-335页

作者：朴丰源孙鲜策刘爽山内彻大连医科大学辽宁大连116027 日本国立三重大学医学部

[目的]摸清东北某城市大气污染颗粒污染物中所含多种有毒重金属的浓度,为防治大气污染提供依据。[方法]用大流量粉尘采样器采样大气颗粒污染物,用电感耦合等离子体质谱仪(ICP-MS)检测大气颗粒物中Cd、Mn、Pb、Ni、Cr和As等6种重金属浓度... 详细信息

[目的]摸清东北某城市大气污染颗粒污染物中所含多种有毒重金属的浓度,为防治大气污染提供依据。[方法]用大流量粉尘采样器采样大气颗粒污染物,用电感耦合等离子体质谱仪(ICP-MS)检测大气颗粒物中Cd、Mn、Pb、Ni、Cr和As等6种重金属浓度。[结果]工业区Cd、Mn、Pb、Ni、Cr和As的浓度分别为9.3、461.9、588.7、69.5、205.7和57.4 ng/m3,交通干线旁为5.7、245.5、305.0、31.4、58.8和32.5 ng/m3,郊区为6.2、154.5、202.3、16.6、26.8和18.2 ng/m3。除Cd浓度外,工业区和交通干线旁的金属浓度显著高于郊区(P<0.05或P<0.01)。6种有毒重金属浓度随时间变化曲线呈现冬、春季浓度高,夏、秋季浓度低的变化规律。[结论]工业区和交通干线旁大气颗粒污染物中的重金属浓度较高。

关键词：大气污染颗粒物质重金属