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In vitro propagation of the endangered medicinal orchid,Dendrobium lasianthera *** through mature seed culture

Asian Pacific Journal of Tropical Biomedicine 2017年第5期7卷 406-410页

作者： Edy Setiti Wida Utami Sucipto Hariyanto yosephine sri wulan manuhara Laboratory of Plant Tissue Culture Department of BiologyFaculty of Sciences and TechnologyAirlangga UniversitySurabayaIndonesia Laboratory of Ecology Department of BiologyFaculty of Sciences and TechnologyAirlangga UniversitySurabayaIndonesia

Objective:To study asymbiotic seed germination and mass propagation of Dendrobium lasianthera which is one of the endangered medicinal orchids using ***:The 14 weeks old hand pollinated seeds were sown on Vacin and Went(VW)solid medium supplemented with various concentrations of peptone(1,2,3 g/L) and without peptone which was used as control *** the 4,8,and 12 weeks after the seeds were sown,seed germination and shoot formation were *** evaluate the role of organic nutrient additives on subsequent shoot development and root formation,particular shoots with about 1 cm length contains 1–2 leaves obtained from the seeds germination was cultured on VW medium additives with different of organic nutrient:15% coconut water,2 g/L peptone,150 g/L banana homogenate,and without organic nutrient was used as *** 16 weeks of culture,the plantlet height,number of leaves,number of roots,leaf length and root length were ***:The supplementation of 2 g/L peptone in VW medium was proven to be suitable concentration for seed germination(100%) and shoot formation with 84.0% the protocorm development to phase 5(shoot).VW medium containing 15% coconut water was effectively improved the shoot development,with well developed roots and leaves compared to the other treatment and 95% of acclimatized plantlets ***:This protocol is an efficient way for the in vitro mass propagation of this Dendrobium lasianthera.

关键词： Dendrobium lasianthera J.J.Sm Micropropagation Seed culture Organic nutrient

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Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor

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Asian Pacific Journal of Tropical Biomedicine 2015年第12期5卷 1027-1032页

作者： yosephine sri wulan manuhara Alfinda Novi Kristanti Edy Setiti Wida Utami Arif Yachya Laboratory of Plant Tissue Culture Biology Department Faculty of Science and Technology Airlangga University Laboratory of Organic Chemistry Chemistry Department Faculty of Science and Technology Airlangga University Laboratory of Plant Tissue Culture Biology Department Faculty of Mathematics and Natural Sciences PGRI Adi Buana University

Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(***)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of *** were infected by Agrobacterium rhizogenes strain *** hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L *** BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for *** and dry weights of hairy roots at the end of culture were ***:Various concentrations of sucrose influenced the biomass accumulation of hairy *** biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than *** supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than ***,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of ***:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of *** in the large scale.

关键词： Talinum paniculatum Gaertn. Saponin Hairy root Liq

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