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Comparative Study of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Culture Test for <i>Candida</i>Identification

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Open Journal of Stomatology 2019年第12期9卷 295-306页

作者： yoriko kubota Chieko Taguchi Masanori Saito Noriko Shinozaki-Kuwahara Tomoko Suzuki Masaaki Suemitsu Mitsuko Nakayama Tadahiko Utsunomiya Hiroyasu Endo Kayo Kuyama Department of Pathology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Microbiology and Immunology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Community Oral Health Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Diagnosis Nihon University School of Dentistry at Matsudo Chiba Japan

Background: A new microorganism identification method using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) has been developed, however, reports on its use for delineating Candida spp. are scarce. Objectives: The purpose of this study was to compare the identification accuracy of mixed infection between culture test and MALDI-TOF MS. Materials and Methods: Eighty-nine denture wearers (average 74.0 ± 9 years) were selected. Specimens were immediately inoculated onto selective medium for CHROMagarTM Candida, and were also carried out using MALDI-TOF MS. The distribution frequencies of them were analyzed. Results: The numbers and rates of detection/non-detection by MALDI-TOF MS of genus Candida were 58/31 (65.2%/34.8%), respectively. Infection types were single infection in 34 (38.2%), mixed infection in 24 (27.0%), and non-infection in 31 (34.8%) cases. Concerning the single infection, C. albicans was the most predominant (58.8%), followed by C. parapsilosis (17.6%), C. glabrata (14.7%), C. tropicalis (5.9%), and C. krusei (2.9%). As for the mixed infection, the most frequent combination was C. albicans and C. glabrata (50.0%), followed by C. albicans and C. parapsilosis (29.2%), C. albicans and C. tropicalis (8.3%), C. glabrata and C. tropicalis (4.2%), C. albicans, C. glabrata, and C. parapsilosis (4.2%), and C. albicans, C. parapsilosis, and C. glabrata (4.2%). There were four MALDI-TOF MS positive results that were negative by the culture test. Conversely, there were six MALDI-TOF MS negative results that were positive by the culture test. The sensitivity and specificity of MALDI-TOF MS were 0.929 and 0.840, respectively. The concordance rate of genus Candida was 0.644, indicating substantial agreement. Conclusion: Candida infection is complicated by disease type and oral cavity environment changes due to aging. A rapid microorganism detection method, such as MALDI-TOF MS, will be helpful to quickly determine the causative pathogen in

关键词： Candida Culture Test MALDI-TOF MS Non-C. Albicans Candida (NCAC)

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