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Involvement of sulfur assimilation in the low β subunit content of soybean seed storage protein revealed by comparative transcriptome analysis

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The Crop Journal 2019年第4期7卷 504-515页

作者： Xi zhang Ruixin Xu Wei Hu Wan Wang Dezhi Han Fan zhang yongzhe Gu Yong Guo Jun Wang Lijuan Qiu College of Agriculture Yangtze University Jingzhou 434025 Hubei China National Key Facility for Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China Soybean Research Center Heihe Institute Heilongjiang Academy of Agricultural Sciences Heihe 100142 Helongjiang China

The β subunit of soybean [Glycine max (L.) Merr.] seed storage protein is of great significance in sulfur-containing amino acid balance and soybean processing properties. The objective of this study was to elucidate the relationship between the β subunit and sulfur-containing amino acid composition, and the potential regulatory mechanism. The β subunit was independently accumulated in comparison with other major subunits (α/α′, acidic, basic, and A3) during seed filling, and a low level of β subunit content (BSC) was formed during the accumulation process. In low-BSC mature seeds, crude protein, oil content, and fatty acid composition were not changed, but sulfur-containing amino acids (Cys + Met) in the low- BSC seeds increased significantly (by 31.5%), suggesting that an internal regulatory mechanism within seed might be responsible for the rebalance of seed protein composition and that sulfur assimilation might be deeply involved in β subunit accumulation. Transcriptomic analysis revealed that genes involved in anabolism of cysteine, methionine, and glutathione were up-regulated but those involved in the catabolism of these compounds were down-regulated, suggesting a relationship between the elevation of methionine and glutathione and low BSC. Our study sheds light on seed composition in low BSC lines and on the potential molecular regulatory mechanism of β subunit accumulation, broadening our understanding of soybean seed protein synthesis and its regulation.

关键词： Soybean β subunit Transcriptome analysis Sulfur-containing amino acids Sulfur assimilation

SinoDuplex: An Improved Duplex Sequencing Approach to Detect Low-frequency Variants in Plasma cfDNA Samples

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Genomics, Proteomics & Bioinformatics 2020年第1期18卷 81-90页

作者： yongzhe Ren Yang zhang Dandan Wang Fengying Liu Ying Fu Shaohua Xiang Li Su Jiancheng Li Heng Dai Bingding Huang College of Big Data and Internet Shenzhen Technology UniversityShenzhen 518118China Department of Research and Development Sinotech Genomics Inc.Kanxing Road 3399Shanghai 201314China Department of Integrated Traditional and Western Medicine In Oncology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Department of Radiation Oncology Fujian Medical University Cancer Hospital and Fujian Cancer HospitalFuzhou 350014China Institute of Synthetic Biology Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhen 518005China

Accurate detection of low frequency mutations from plasma cell-free DNA in blood using targeted next generation sequencing technology has shown promising benefits in clinical *** sequencing technology is the most commonly used approach in liquid *** molecular identifiers are attached to each double-stranded DNA template,followed by production of low-error consensus sequences to detect low frequency ***,high sequencing costs have hindered application of this approach in clinical ***,we have developed an improved duplex sequencing approach called Sino Duplex,which utilizes a pool of adapters containing pre-defined barcode sequences to generate far fewer barcode combinations than with random sequences,and implemented a novel computational analysis algorithm to generate duplex consensus sequences more *** Duplex increased the output of duplex sequencing technology,making it more *** evaluated our approach using reference standard samples and cell-free DNA samples from lung cancer *** results showed that Sino Duplex has high sensitivity and specificity in detecting very low allele frequency *** source code for Sino Duplex is freely available at https://***/Sin Oncology/sinoduplex.

关键词： Next generation sequencing Liquid biopsy Circulating tumor DNA Duplex sequencing Low frequency variant

Antibodies to phosphatidylserine/prothrombin(aPS/PT) enhanced the diagnostic performance in Chinese patients with antiphospholipid syndrome

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Antibodies to phosphatidylserine/prothrombin(aPS/PT) enhance...

Rydberg state excitation in molecules manipulated by bicircular two-color laser pulses

第十三届全国免疫学学术大会

作者： Shulan zhang yongzhe Li Peking Union Medical College Hospital

Background: Increasing evidence has highlighted the role of non-criteria aPLs as important supplements to the current criteria aPLs in *** this retrospective study, we evaluated the clinical relevance of antibodies to phosphatidylserine/prothrombin(aPS/PT) in Chinese patients with ***: A total of 441 subjects were tested, including 101 patients with primary APS(PAPS), 140 patients with secondary APS(SAPS), 161 disease controls(DCs) and 39 healthy controls(HCs).Serum IgG/IgM aPS/PT were determined by ***: The levels of IgG/IgM aPS/PT were significantly increased in patients with APS compared with DCs and *** and IgM aPS/PT were present in 29.7% and 54.5% of PAPS, and 42.1% and 53.6% of SAPS, *** diagnosis of APS, IgG aCL exhibited the highest positive likelihood ratio(LR+) of 21.60, followed by LA(13.84), IgG aβ2GP1(9.19) and IgG aPS/PT(8.49).aPS/PT were detected in 13.3% of seronegative PAPS patients and 31.3% of seronegative SAPS *** exhibited the highest OR of 3.64 in identifying patients with thrombosis, followed by IgG aCL(OR, 2.63), IgG aPS/PT(OR, 2.55) and IgG aβ2GP1(OR, 2.33).LA and IgG aCL were correlated with both arterial and venous thrombosis, whereas IgG aPS/PT and IgG aβ2GP1 correlated with venous or arterial thrombosis, ***: Our findings suggest that the inclusion of IgG/IgM aPS/PT may enhance the diagnostic performance for APS, especially in those in whom APS is highly suspected, but conventional aPLs are repeatedly *** addition, IgG aPS/PT may contribute to identify patients at risk of thrombosis.

关键词： Antiphospholipid syndrome(APS) Antiphospholipid antibodies APS/PT thrombosis Seronegative APS

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Advanced Photonics 2023年第1期5卷 19-26页

作者： Wenbin zhang yongzhe Ma Chenxu Lu Fei Chen Shengzhe Pan Peifen Lu Hongcheng Ni Jian Wu East China Normal University State Key Laboratory of Precision SpectroscopyShanghaiChina Ludwig-Maximilians-Universität Munich Department of PhysicsGarchingGermany Max Planck Institute of Quantum Optics GarchingGermany Shanxi University Collaborative Innovation Center of Extreme OpticsTaiyuanChina CAS Center for Excellence in Ultra-Intense Laser Science ShanghaiChina

Multiphoton resonant excitation and frustrated tunneling ionization,manifesting the photonic and optical nature of the driving light via direct excitation and electron recapture,respectively,are complementary mechanisms to access Rydberg state excitation(RSE)of atoms and molecules in an intense laser ***,clear identification and manipulation of their individual contributions in the light-induced RSE process remain experimentally ***,we bridge this gap by exploring the dissociative and nondissociative RSE of H2 molecules using bicircular two-color laser *** on the relative field strength and polarization helicity of the two colors,the RSE probability can be boosted by more than one order of magnitude by exploiting the laser waveform-dependent field *** role of the photon effect is readily strengthened with increasing relative strength of the second-harmonic field of the two colors regardless of the polarization *** compared to the nondissociative RSE forming H2,the field effect in producing the dissociative RSE channel of eHt;HT is moderately suppressed,which is primarily accessed via a three-step sequential process separated by molecular bond *** work paves the way toward a comprehensive understanding of the interplay of the underlying field and photon effects in the strong-field RSE process,as well as facilitating the generation of Rydberg states optimized with tailored characteristics.

关键词： strong-field Rydberg state excitation molecular dissociative ionization bicircular two-color fields multiphoton resonant excitation frustrated tunneling ionization

机构投资者持股持续性对我国上市公司业绩及风险的影响研究

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系统工程理论与实践 2018年第2期38卷 273-286页

作者：张涤新屈永哲南京大学商学院

通过构建机构持股持续性指标体系，从四个维度全面研究机构持股持续性对上市公司业绩及风险的影响。整体来看，机构持股比例和持股稳定性的提升显著提高了公司业绩并降低了风险，反之则相反；机构持股期限与公司业绩仅呈很弱的显著负相... 详细信息

通过构建机构持股持续性指标体系，从四个维度全面研究机构持股持续性对上市公司业绩及风险的影响。整体来看，机构持股比例和持股稳定性的提升显著提高了公司业绩并降低了风险，反之则相反；机构持股期限与公司业绩仅呈很弱的显著负相关，导致这一异象的原因是：机构持股期限仅是一种表象，其背后隐含的是机构频繁换股的“散户化”行为及其不同的利益诉求带来了摩擦，加剧了公司经营决策和管理的不确定性，使公司业绩下降．分组研究显示：机构持股对公司业绩及风险的影响存在异质性；机构投资者只有秉持长期价值投资才利于公司业绩的提升，而以短期投机为目的的机构投资者对公司发展有害无益；机构持股稳定性越高，越有利于降低公司业绩风险；在机构持股比例高且持股期限长的公司中，机构持股比例与期限的协同效应越强，对公司业绩的改善作用越大，反之越小；在机构低比例持股的公司中，机构持股稳定性的提升对降低公司业绩风险的作用大于机构高比例持股的公司中对应的作用．

关键词：机构投资者持股持续性公司业绩及风险面板数据模型

Welding anomaly detection based on supervised learning and unsupervised learning

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China Welding 2022年第3期31卷 24-29页

作者： Fa yongzhe zhang Baoxin Ya Wei Rook Remco Mahadevan Gautham Tulini Isotta Yu Xinghua School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081 China Rotterdam Additive Manufacturing Laboratory Rotterdam 401147 Netherlands

In order to solve the problem of automatic defect detection and process control in the welding and arc additive process,the paper monitors the current,voltage,audio,and other data during the welding process and extracts the minimum value,standard deviation,deviation from the voltage and current *** extracts spectral features such as root mean square,spectral centroid,and zero-crossing rate from audio data,fuses the features extracted from multiple sensor signals,and establishes multiple machine learning supervised and unsupervised *** are used to detect abnormalities in the welding *** experimental results show that the established multiple machine learning models have high accuracy,among which the supervised learning model,the balanced accuracy of Ada boost is 0.957,and the unsupervised learning model Isolation Forest has a balanced accuracy of 0.909.

关键词： welding anomaly detection machine learning unsupervised learning supervised learning

Dynamic landscape mapping of humoral immunity to SARS-CoY-2 identifies non-structural protein antibodies associated with the survival of critical COVID-19 patients

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Signal Transduction and Targeted Therapy 2021年第9期6卷 2843-2856页

作者： Linlin Cheng Xiaomei zhang Yu Chen Dan Wang Dong zhang Songxin Yan Hongye Wang Meng Xiao Te Liang Haolong Li Meng Xu Xin Hou jiayu Dai Xian Wu Mingyuan Li Minya Lu Dong Wu Ran Tian Jing Zhao Yan zhang Wei Cao Jinglan Wang Xiaowei Yan Xiang Zhou Zhengyin Liu Yingchun Xu Fuchu He yongzhe Li Xiaobo Yu Shuyang zhang Department of Clinical Laboratory State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina State Key Laboratory of Proteomics Beijing Proteome Research CenterNational Center for Protein Sciences-Beijing(PHOENIX Center)Beijing Institute of LifeomicsBeijingChina

A comprehensive analysis of the humoral immune response to the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is essential in understanding COVID-19 pathogenesis and developing antibody-based diagnostics and *** this work,we performed a longitudinal analysis of antibody responses to SARS-CoV-2 proteins in 104 serum samples from 49 critical COVID-19 patients using a peptide-based SARS-CoV-2 proteome *** data show that the binding epitopes of IgM and IgG antibodies differ across SARS-CoV-2 proteins and even within the same protein.

关键词： immunity antibodies patients

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自身免疫性心肌病相关自身抗体谱研究进展

中华检验医学杂志 2023年第11期46卷 1205-1210页

作者：张婧頔李永哲中国医学科学院、北京协和医学院、北京协和医院检验科北京100730

自身免疫性心肌病是一类由自身免疫介导的异质性心肌疾病,其具体发病机制尚未明确。多种自身抗体已在自身免疫性心肌病患者的血清中检出,相关自身抗体谱的研究有助于了解疾病的发病机制,提高疾病筛查和诊断效能、监测疾病进展、评估预... 详细信息

自身免疫性心肌病是一类由自身免疫介导的异质性心肌疾病,其具体发病机制尚未明确。多种自身抗体已在自身免疫性心肌病患者的血清中检出,相关自身抗体谱的研究有助于了解疾病的发病机制,提高疾病筛查和诊断效能、监测疾病进展、评估预后和治疗效果等。本文将总结自身免疫性心肌病中相关自身抗体谱的研究进展及临床应用,以为临床诊治提供参考。

关键词：心肌病自身免疫自身抗体自身抗原

原发性失眠患者易损脑区的静息态功能磁共振成像研究:一项基于激活似然估计法的Meta分析

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磁共振成像 2022年第6期13卷 88-93页

作者：张琴侯勇哲张伟王琳甘肃中医药大学第一临床医学院兰州730000 甘肃省中西医结合肿瘤临床医学研究中心甘肃中医药大学附属医院放射科兰州730000

目的探讨应用静息态功能磁共振成像(resting state functional magnetic resonance imaging,rs-fMRI)技术研究原发性失眠(primary insomnia,PI)患者较为一致的易损脑区,从而揭示PI脑损伤可能的神经机制。材料与方法检索2022年4月7日之... 详细信息

目的探讨应用静息态功能磁共振成像(resting state functional magnetic resonance imaging,rs-fMRI)技术研究原发性失眠(primary insomnia,PI)患者较为一致的易损脑区,从而揭示PI脑损伤可能的神经机制。材料与方法检索2022年4月7日之前采用局部一致性(regional honogeneity,ReHo)及低频振幅/比率低频振幅/动态低频振幅(amplitude of low-frequency fluctuation/fraction amplitude of low-frequency fluctuation/dynamic amplitude of low-frequency fluctuation,ALFF/fALFF/dALFF)分析方法探讨PI患者脑功能改变的文献,按照严格的纳排标准,采用激活似然估计法(activation likelihood estimation,ALE)对既往研究中PI患者相对于健康对照组(healthy controls,HCs)自发神经活动异常的脑区进行整合分析。结果共纳入19篇文献20个研究(PI 706例,HCs 681例)。结合ReHo及ALFF/fALFF/dALFF数据元分析结果显示,PI患者相对于HCs左侧梭状回和海马旁回活动增加(体素分别为3640、928 mm3,P<0.05),并未发现活动减低的脑区。单独ALFF数据元分析结果显示,PI患者相对于HCs左侧梭状回活动增加(体素为1360 mm3,P<0.05),并未发现活动减低的脑区。单独ReHo数据元分析结果显示,PI患者相对于HCs并未出现任何活动增高或减低的脑区。结论本文通过ALE元分析发现PI患者较为一致的易损脑区为左侧梭状回和海马旁回,这有助于进一步从神经病理学的角度理解PI脑损伤。

关键词：静息态功能磁共振成像原发性失眠 Meta分析激活似然估计法自发神经活动易损脑区