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Transcriptional Analysis of Pseudomonas stutzeri A1501 Associated with Host Rice

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Advances in Microbiology 2016年第3期6卷 210-221页

作者： Khandakar Mohiul Alam Tao Zhang yonglian Yan Wei Zhang Min Lin Wei lu Bangladesh Sugarcrop Research Institute Ishurdi Bangladesh Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing China

Pseudomonas stutzeriA1501, associative and endophytic nitrogen-fixing bacterium showed the capacity of colonization in the rice roots and considered as the good colonizer in the rice plant. The experiment was conducted to study the expression of genes potentiality relevant to the association of nitrogen fixing Pseudomonas stutzeri with host rice and reveal the molecular mechanism by which underlying interaction between bacteria and host rice. The bacteria were shown to be uniformly distributed on the rhizoplane of the root and the density of bacteria was found at the intercellular junction and micro colony developed on the surface of the epidermal cells and on the cellular junctions. Root exudates of rice were the major components of carbon and energy sources for bacteria. RT-PCR analyses of pilK, metE, rpoN and fdhE genes expression of P. stutzeri A1501 were performed at positive and negative (control) conditions. After 1 h, it was found that pilK, metE and rpoN transcription were increased 5.7, 6.4 and 3.4-fold, respectively, whereas in the fdhE gene has no expression. Consequently, after 4 h pilk, fdhE, metE and rpoN were decreased -1.9, -4.4, -0.2 and -0.8-fold, respectively. The gene pilK, expression was up-regulation after 1 h and down-regulation after 4 h that has twitching motility to convey the bacterial cell to point of attachment in to host plant. The gene expressions of the bacteria, pilK, metE, rpoN and fdhE were up- and down-regulated during the influence of root exudates which regulated the colonization of bacteria during plant-microbe interaction.

关键词： Rice Pseudomonas stutzeri A1501 Transcriptional Analysis Root Exudates Colonization

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晚清时期维护东沙群岛主权的民间力量

海南热带海洋学院学报 2022年第1期29卷 4-11页

作者：刘永连卢玉敏暨南大学文学院广州510632 暨南大学中外关系研究所广州510632 暨南大学图书馆广州510632

1908—1909年,日本商人西泽吉次窃据东沙群岛和盗采我国海洋资源的活动,引起了广东地方政府及民间力量的高度重视和极力抗争。其中,新闻界宣传报道,积极推动舆论,政绅商学各界集会倡议,呼吁维护主权,更有广大民众掀起风潮,抗议日本帝国... 详细信息

1908—1909年,日本商人西泽吉次窃据东沙群岛和盗采我国海洋资源的活动,引起了广东地方政府及民间力量的高度重视和极力抗争。其中,新闻界宣传报道,积极推动舆论,政绅商学各界集会倡议,呼吁维护主权,更有广大民众掀起风潮,抗议日本帝国主义侵占我领土之罪行并以抵制日货等方式声援对外交涉,推动我国政府顺利收复东沙群岛主权。

关键词：晚清东沙群岛主权维护新闻舆论民众抗议

A sequence of 28S rRNA-derived small RNAs is enriched in mature sperm and various somatic tissues and possibly associates with inflammation

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Journal of Molecular Cell Biology 2017年第3期9卷 256-259页

作者： Chu, Chen Yu, lu Wu, Bin Ma, Li Gou, Lan-Tao He, Miao Guo, Yunli Li, Zhi-Tong Gao, Wei Shi, Huijuan Liu, Mo-Fang Wang, Hongyan Chen, Charlie Degui Drevet, Joel R. Zhou, Yuchuan Zhang, yonglian Univ Chinese Acad Sci Chinese Acad Sci Shanghai Inst Biochem & Cell BiolState Key Lab M CAS Ctr Excellence Mol Cell SciShanghai Key Lab Shanghai 200031 Peoples R China Fudan Univ SIPPR China Natl Populat & Family Planning Key Lab Cont Shanghai 200032 Peoples R China Univ Chinese Acad Sci Chinese Acad Sci Shanghai Inst Biochem & Cell Biol CAS Ctr Excellence Mol Cell SciState Key Lab Mol Shanghai 200031 Peoples R China Univ Calif San Diego Dept Cellular & Mol Med San Diego CA 92093 USA Chinese Acad Sci Shanghai Inst Biol Sci State Key Lab Neurosci Inst Neurosci Shanghai 200031 Peoples R China Shanghai Jiao Tong Univ Shanghai Gen Hosp Dept Geratol Shanghai 200080 Peoples R China Univ Clermont Auvergne CNRS UMR6293 INSERM U1103 Genet Reprod & Dev Lab Clermont Ferrand France

The growing understanding concerning the definition, classification, and function of small non-coding RNAs (sncRNAs) has led to the discovery of new sncRNA species of various sizes. Recent studies highlighted a group of tsRNAs derived from the 5' end of tRNAs (Peng et al., 2012; Zhang et al., 2014; Chen et al., 2016; Sharma et al., 2016), which raises the question whether there are similar small RNAs derived from other housekeeping RNAs, such as rRNAs.

关键词：小RNA rRNA 28S 体细胞炎症精子成熟

磷酸铁锂电池储能电站消防排水水质分析及工程对策

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给水排水 2023年第11期49卷 114-119页

作者：蔡兴初杨鹏熊永莲陆勇泽巩峰中国能源建设集团江苏省电力设计院有限公司南京211102 东南大学能源与环境学院南京210089 盐城工学院汽车工程学院盐城224051

以磷酸铁锂电池为储能介质的化学储能电站火灾风险高,采用消防水对其灭火时会有大量的消防废水排出。磷酸铁锂电池由电解质锂盐、电解液等组成,成分复杂,目前缺乏消防排水的水质研究,在实际工程中对消防排水的出路存在争议。针对不同新... 详细信息

以磷酸铁锂电池为储能介质的化学储能电站火灾风险高,采用消防水对其灭火时会有大量的消防废水排出。磷酸铁锂电池由电解质锂盐、电解液等组成,成分复杂,目前缺乏消防排水的水质研究,在实际工程中对消防排水的出路存在争议。针对不同新旧程度、不同充电程度的100 Ah容量磷酸铁锂电池开展燃烧及灭火实验,收集其消防排水并检测,检测表明:消防排水为中性液体;排水中重金属(铜、锌、镍、镉、铅、锰等)、砷、总氮、氨氮未超过相关排放标准;排水中氟离子、总磷、COD数值较高。将实验数据和工程设计参数对比:磷酸铁锂电池储能电站火灾消防排水具有大部分污染物指标低于相关排放标准,部分指标偏高但污染物总量相对不大等特点,在火灾状态应急外排不会造成较大的环境污染;对于建设于环保要求较高区域的储能电站,建议将废水收集后自然风干或外运处理。

关键词：磷酸铁锂电池消防排水水质

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高海拔地区绿菜花春茬露地栽培技术

农业科技通讯 2008年第5期 136-137页

作者：卢永莲青海省湟中县农业技术推广中心 811600

根据高海拔地区的气候特点和绿菜花作物特性,从其对温度、光照、水分及肥料的要求,介绍了高海拔地区绿菜花春茬露地栽培技术,以利指导生产。

关键词：高海拔绿菜花栽培技术

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贵州山地杂交籼稻区水分时空变化规律研究

南京农业大学学报 2022年第3期45卷 453-464页

作者：江学海李刚华陆波罗德强刘正辉李敏蒋明金陈永莲丁艳锋南京农业大学农业农村部作物生理生态与生产管理重点实验室/江苏现代作物生产协同创新中心江苏南京210095 贵州省水稻研究所贵州贵阳550006 麻江县农业技术推广站贵州麻江557600

[目的]研究贵州山地不同杂交籼稻生态区水分亏缺的时空分布特征,为水稻生产区域水分管理策略提供理论支持。[方法]采用气象资料分析和田间试验结合的方法,在贵州5个杂交籼稻生态稻区,收集了1986—2015年30 a的气温、降水等气象资料,并... 详细信息

[目的]研究贵州山地不同杂交籼稻生态区水分亏缺的时空分布特征,为水稻生产区域水分管理策略提供理论支持。[方法]采用气象资料分析和田间试验结合的方法,在贵州5个杂交籼稻生态稻区,收集了1986—2015年30 a的气温、降水等气象资料,并在每一生态区的代表试点进行杂交籼稻生育期试验,按有效分蘖、无效分蘖、孕穗开花和灌浆成熟4个阶段分析需水量、有效降水量和水分时空变化规律。[结果]30 a贵州5个杂交籼稻生态稻区的安全播种期呈现提前的趋势,黔南、黔东、黔北、黔西南和黔中稻区安全播种期的倾向率分别为-6.5、-5.4、-4.9、-4.7和-4.1 d·10 a^(-1);5个生态区杂交籼稻的有效分蘖、无效分蘖、孕穗开花和灌浆成熟阶段时长相差6.0~17.2 d,黔西南稻区无效分蘖阶段较长、黔南稻区灌浆成熟阶段较短。本田期参考作物蒸散量(ET_(0))由东向西增高,黔西南稻区(612.5 mm)高于其他稻区,30 a杂交籼稻生长前两阶段ET_(0)呈下降趋势,后两阶段ET_(0)呈升高的趋势;本田期有效降水量(P_(e))由东向西增加,黔西南稻区无效分蘖阶段P_(e)高于其他稻区,孕穗开花、灌浆成熟阶段P_(e)随时间呈下降趋势;需水量(ET_(c))分布在空间上的差异大于时间,5个生态稻区水分亏缺(WD)与有效降水量呈负相关,有效分蘖和无效分蘖阶段WD由东向西南升高、孕穗开花和灌浆成熟阶段WD由南向北升高;有效分蘖、孕穗开花阶段WD分别以黔西南和黔中高于其他稻区;各稻区生殖生长阶段的降水协同指数(C_(d))低于营养生长阶段,黔北、黔东和黔中稻区孕穗开花阶段的C_(d)较低。[结论]黔西南稻区有效分蘖阶段水分亏缺较大;黔北、黔东和黔中稻区的孕穗开花阶段水分亏缺较大,降水协同指数降低,因水分亏缺而减产的风险加大。

关键词：气候变化杂交籼稻生育时期水分亏缺时空变化

Clinical efficacy of low-dose emetine for patients with COVID-19:a real-world study

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Journal of Bio-X Research 2021年第2期4卷 53-59页

作者： Song Fan Qi Zhen Cheng Chen Wenjun Wang Qibing Wu Huihui Ma Chengyuan Zhang Li Zhang Baojing lu Huiyao Ge Liang Yong Bao Li Yafen Yu Weiwei Chen Yiwen Mao Guangbo Qu Li Su Aoli Wang Zhen Ding Haiwen Li Jin Zhang yonglian Wang Yufeng Gao Xihai Xu Zhongming Zhu Jun Chen Long Zhang Hongqiang Liang Song Wu Meng Huang Quan Xia Ping Li Yehuan Sun Chaozhao Liang Wei Wei Qingsong Liu Liangdan Sun Department of Urology the First Affiliated Hospital of Anhui Medical UniversityInstitute of UrologyAnhui Medical University and Anhui Province Key Laboratory of Genitourinary Diseases Department of Dermatology the First Affiliated Hospital of Anhui Medical UniversityKey Laboratory of DermatologyAnhui Medical UniversityMinistry of EducationInflammation and Immune Mediated Diseases Laboratory of Anhui Province High Magnetic Field Laboratory Key Laboratory of High Magnetic Field and Ion Beam Physical BiologyHefei Institutes of Physical ScienceChinese Academy of Sciences Department of Radiation Oncology the First Affiliated Hospital of Anhui Medical UniversityHefei Anhui Lixin County Hospital of Traditional Chinese Medicine Bozhou Department of Respiratory Medicine Anhui Feidong County People’s Hospital Center for Scientific Research of the First Affiliated Hospital of Anhui Medical University Department of Microbiology School of Basic Medical ScienceAnhui Medical University Comprehensive Lab College of BasicAnhui Medical University Department of Epidemiology and Health Statistics School of Public HealthAnhui Medical University Traditional and Western Medicine(TCM)-Integrated Cancer Center of Anhui Medical University the Department of Traditional and Western Integrative Medicine Oncologythe First Affiliated Hospital of Anhui Medical University Department of Respiratory Medicine Binhu Hospital of Hefei The Third Affiliated Hospital of Anhui Medical University the First Affiliated Hospital of Zhengzhou University The Second People’s Hospital of Hefei The Department of Infectious Diseases the First Affiliated Hospital of Anhui Medical University The Department Pulmonary and Critical Care Medicine the First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui Province Anhui Suzhou Hospital SuzhouJiangsu Province Anhui Feidong County People’s Hospital Department of Pharmacy the First Affiliated Hospital of Anhui Medical University Institute of Clinical Pharmacology Anhui Medical UniversityKey Laborat

Objective:Emetine,an isoquinoline alkaloid that is enriched at high concentrations in the lung,has shown potent in vitro activity against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The aim of this study was to better understand the effectiveness of low-dose emetine for patients with coronavirus disease 2019(COVID-19).Methods:In this real-world study,63 patients with mild or common COVID-19 were recruited from Wuhan Fangcang Shelter Hospital and five COVID-19-designated hospitals in Anhui Province,China from February to March 2020.Thirty-nine patients from Wuhan Fangcang Shelter Hospital were assigned to a pragmatic randomized controlled clinical trial,and 24 patients from the 5 COVID-19-designated hospitals in Anhui Province underwent a real-world study.The medication course of emetine was less than 10 days.The main symptoms and adverse reactions of all patients were observed and recorded.The primary outcome measure was the time required for a negative SARS-CoV-2 RNA result or the negative result rate on day 10.Secondary outcomes included axillary temperature,transcutaneous oxygen saturation,and respiratory frequency recovery.The study was approved by the Ethics Committee of The First Affiliated Hospital of Anhui Medical University on February 20,2019(approval No.PJ2020-03-19)and was registered with the Chinese Clinical Trial Registry on February 20,2019(registration number:ChiCTR2000030022).Results:The oxygen saturation values were higher in the treatment group than in the control group on the first day after enrollment for patients treated at Fangcang Shelter Hospital.The axillary body temperature,respiratory rate,and oxygen saturation among patients in Fangcang Shelter Hospital were related to the time effect but not to the intervention measures.The respiratory rate and oxygen saturation of patients in the Anhui designated hospitals were related to the intervention measures but not to the time effect.The axillary body temperature of patients in Anhui designated hospitals was related to the tim

关键词： arbidol COVID-19 emetine randomized controlled clinical trial real-world study

沈阳地区水环境和生物样品中全氟化合物的污染分布特征

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环境科学学报 2010年第10期30卷 2097-2107页

作者：杨永亮路国慧杨伟贤潘静谷保佐知山下信义林群声国家地质实验测试中心中国地质科学院生态地球化学重点开放实验室北京100037 海洋污染国家重点实验室香港城市大学生物化学系日本综合产业技术研究所

采用高效液相色谱/电喷雾负电离源串联质谱法对沈阳市各类水环境和生物样品中全氟化合物(PFCs)进行测定,研究了PFCs的河流分布及季节分布特征.同时,对通过饮用水及家禽和鱼摄入全氟辛烷磺酸(PFOS)和全氟辛酸(PFOA)的人体健康风险进行了... 详细信息

采用高效液相色谱/电喷雾负电离源串联质谱法对沈阳市各类水环境和生物样品中全氟化合物(PFCs)进行测定,研究了PFCs的河流分布及季节分布特征.同时,对通过饮用水及家禽和鱼摄入全氟辛烷磺酸(PFOS)和全氟辛酸(PFOA)的人体健康风险进行了评价.结果表明,沈阳市各类水环境样品中总PFCs浓度范围为nd～6.01ng·L-1,平均浓度为5.23ng·L-1.PFOS和PFOA是沈阳地区所有水环境样品中最主要的PFCs污染物,浓度范围分别为nd～2.83ng·L-1和nd～5.71ng·L-1,平均浓度分别为1.11和2.13ng·L-1.细河的PFOS含量最高,约为浑河PFOS含量的2倍,蒲河PFOS含量的3倍.一些PFCs化合物之间呈正相关关系,说明其可能具有相同的来源.PFOS与PFOA之间无相关关系,由此推断沈阳水体中二者具有不同来源.浑河流经沈阳市时,PFOS和PFOA浓度升高.流经沈阳市区后,PFOS和全氟己烷磺酸钾(PFHxS)浓度明显升高.细河中PFHxS也有检出,表明沈阳市是周边水体中PFOS及PFHxS的主要污染来源.丰水期地表水中PFOS和PFOA浓度与枯水期相比没有明显差异,但丰水期水中PFCs组成更为丰富,出现了大量全氟庚酸(PFHpA).沈阳地区自来水中PFOS和PFOA浓度平均值分别为0.39和0.85ng·L-1,最高浓度分别为1.16和2.55ng·L-1,均低于美国饮用水健康参考值.沈阳生物样品中PFOS和全氟十一酸(PFUnDA)是最普遍的化合物.总PFCs平均含量在鱼样品中为6.59ng.g-1(以干重计),鸡和鸭血清样品中分别为1.65和0.69ng·mL-1,鸡和鸭肝脏样品中分别为0.41和1.68ng.g-1(以干重计),与文献报道相比处于较低水平.

关键词：全氟化合物地表水地下水生物样品沈阳

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喷撒技术生产粉末冶金摩擦材料

机械工程材料 1988年第6期 49-51页

作者：杨永连鲁乃光杭州粉末冶金研究所

喷撒技术制取粉末冶金摩擦材料较传统的压制-烧结-机械加工的生产方法,具有许多优点,本文论述了这两种工艺的优缺点及技术指标对比,列举了几种典型喷撒材料的主要技术性能数据、摩擦磨损性能曲线。并指出这一新技术为某些双金属材料及... 详细信息

喷撒技术制取粉末冶金摩擦材料较传统的压制-烧结-机械加工的生产方法,具有许多优点,本文论述了这两种工艺的优缺点及技术指标对比,列举了几种典型喷撒材料的主要技术性能数据、摩擦磨损性能曲线。并指出这一新技术为某些双金属材料及复合材料以优质、高效、低成本进行生产提供了有价值的依据。

关键词：粉末冶金摩擦材料机械加工摩擦层大气边界层