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Effects of electrostimulation and administration of succinylcholine on the expression of Fos protein in mesencephalic periaqueductal gray matter of rats after simulated weightlessness

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Neural Regeneration Research 2006年第1期1卷 22-25页

作者： yongjin zhu Sudi Wu Xiaoli Fan Xinai Song Linping Xu Department of Physiology Medical School Xi＇an Jiaotong University Xi＇an 710061 Shaanxi Province China Key Laboratory of Environment and Genes Related to Diseases Xi＇an Jiaotong University Min istry of Education Xi＇an 710061. Shaanxi Province China

BACKGROUND： Expression of Fos in neurons of periaqueductal gray （PAG） is used to reflect the excitability. However, changes of expression of Fos in neurons of PAG are caused by injured electrostimulation after simulated weightlessness, and the relationship between pretreatment and injection of succinylcholine has not been determined yet. OBJECTIVE ： To investigate the changes of expression of Fos in PAG induced by injured electrostimulation pretreatment and injection of succinylcholine at 2 weeks after simulated weightlessness.DESIGN： Observational and controlled animal study.SETTING： Department of Physiology, Medical School, Xi＇an Jiaotong University; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education. MATERIALS： A total of 24 adult female SD rats, of clean grade and weighing 180-220 g, were selected in this study. METHODS： The experiment was completed in the Experimental Animal Center of Xi＇an Jiaotong University.① All rats were randomly divided into 2 groups according to body mass： simulated weightlessness group and control group with 12 in each group. And then, each group was also divided into 3 subgroups： electrostimulation group, succinylcholine-pretreatment group and succinylcholine-injection group with 4 in each subgroup. ②The model of weightlessness was simulated by tail-suspended female rats, which were described and modified by Cheng Jie. Rats in normal control group were given the same interventions as simulated weightlessness group except for tail-suspended. ③ Experimental method： The rats in electrostimulation group were given nociceptive stimulus by a pair of subcutaneous electrodes inserted into 1 and 5 claw of left hindlimb. The stimulus （current： 10 mA; duration： 1 ms; interval： 1 s） lasted for 30 minutes. The rats in succinylcholine-pretreatment group received stimulus after intravenous administration of succinylcholine, rats in succinylcholine-injection group were not given stimulus, just received succiny

关键词： Fos Effects of electrostimulation and administration of succinylcholine on the expression of Fos protein in mesencephalic periaqueductal gray matter of rats after simulated weightlessness

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RNAi knockdown of C-erbB2 expression inhibits salivary gland adenoid cystic carcinoma SACC-83 cell growthin vitro

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The Journal of Biomedical Research 2010年第3期24卷 215-222页

作者： Xiaohua Liu Yincheng Zhang Wenhao Ren Tengteng Cao yongjin zhu Department of Oral and Maxillofacial Surgery Xi'an Jiaotong University Stomatology Hospital Xi 'an 710004 Shaanxi Province China Department of Center Research Xi'an Jiaotong University Stomatology Hospital Xi'an 710004 Shaanxi Province China

Objective：To knockdown the C-erbB2 gene in salivary gland adenoid cystic carcinoma SACC-83 cells using RNA interference,and determine the effect of silencing C-erbB2 on cell ***：C-erbB2-siRNA was transfected into SACC-83 ***-PCR and immunohistochemistry were used to detect C-erbB2 expression in SACC-83 *** proliferation was measured by the MTT assay and gene knockdown was achieved by RNA *** was analyzed by flow ***：Compared with the control,C-erbB2 mRNA expression was decreased in the C-erbB2-siRNA transfection group,and immunohistochemical analysis indicated that C-erbB2 protein expression was *** C-erbB2-siRNA was transfected for 48 h,absorbance at 570 nm （MTT）was 0.185±0.021 compared with 0.354±0.034,0.299±0.053,and 0.314±0.049 in the blank control,liposome control and negative control siRNA groups,*** differences were statistically significant （P〈0.05）between the C-erbB2-siRNA group and the control *** the C-erbB2 knockdown,the percentage of apoptotic cells was 5.63%compared with 2.04%,2.85%,and 2.98%in the three control groups,*** of SACC-83 cells was inhibited,and early apoptotic cells were ***： RNA interference can effectively silence C-erbB2 gene expression and inhibit growth of SACC-83 cells,which indicates the potential of targeting this gene as a novel gene therapy approach for the treatment of salivary gland adenoid cystic carcinoma.

关键词： salivary gland adenoid cystic carcinoma RNA interference C-erbB2 gene silence

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Reservoir geological modeling and significance of Cambrian Xiaoerblak Formation in Keping outcrop area, Tarim Basin, NW China

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Petroleum Exploration and Development 2020年第3期47卷 536-547页

作者： ZHENG Jianfeng PAN Wenqing SHEN Anjiang YUAN Wenfang HUANG Lili NI Xinfeng zhu yongjin Key Laboratory of Carbonate Reservoir CNPCHangzhou 310023China PetroChina Hangzhou Research Institute of Geology Hangzhou 310023China Tarim Oilfield Company PetroChinaKorla 841000China

Take the Cambrian Xiaoerblak Formation in the Keping(Kalpin) outcrop area as an example, a 28 km reservoir scale geological model was built based on description of 7 profiles, observation of more than 1000 thin sections, petrophysical analysis of 556 samples and many geochemical tests. The Xiaoerblak Formation, 158–178 m thick, is divided into three members and 5 submembers, and is composed of laminated microbialite dolomite(LMD), thrombolite dolomite(TD), foamy-stromatolite dolomite(FSD), oncolite dolomite(OD), grain dolomite(GD)/crystalline dolomite with grain ghost and micritic dolomite(MD)/argillaceous dolomite. The petrology features show that its sediment sequence is micro-organism layer – microbial mound/shoal – tidal flat in carbonate ramp background from bottom up. The reservoir has 5 types of pores, namely, framework pore, dissolved vug, intergranular and intragranular dissolved pore and intercrystalline dissolved pore, as main reservoir space. It is found that the development of pore has high lithofacies selectivity, FSD has the highest average porosity, TD, OD and GD come second. The reservoir is pore-vug reservoir with medium-high porosity and medium-low permeability. The dolomite of Xiaoerblak Formation was formed in para-syngenetic to early diagenetic stage through dolomitization caused by seawater. The reservoir development is jointly controlled by sedimentary facies, micro-organism type, high frequency sequence interface and early dolomitization. The classⅠand Ⅱ reservoirs, with an average thickness of 41.2 m and average reservoir-stratum ratio of about 25.6%, have significant potential. It is predicted that the microbial mounds and shoals in the middle ramp around the ancient uplift are the favorable zones for reservoir development.

关键词： Tarim Basin Keping area Xiaoerblak Formation dolomite microbialite reservoir genesis geological modeling

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A New Neighborhood Union Condition for Hamiltonian Graphs

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Acta Mathematica Sinica,English Series 1997年第2期13卷 187-192页

作者： Wei Bing zhu yongjin (Institute of Systems Science,Academia Sinica,Beijing 100080,China) Institute of Systems Science Academia Sinica Beijing China

For a vertex set{u1,u2,…,uk}of a graph G with n vertices,let s（G;{u1,u2,…,uk}）=Σ1≤i≤j≤k|N（ui）UN（uj）|, NCk.=min{s（G;{x1,…,xk}）:{x1,…,xk}is an independent set}. In this paper,we shall prove that if G is 3-connected and NC4≥3n,then G is either a hamiltonian or Petersen *** generalizes some results on the neighborhood union conditions for hamiltonian graphs.

关键词： Neighborhood unions Insertible vertex Hamiltonian graphs

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Tectonic-lithofacies paleogeography,large-scale source-reservoir distribution and exploration zones of Cambrian subsalt formation,Tarim Basin,NW China

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Petroleum Exploration and Development 2021年第6期48卷 1289-1303页

作者： WEI Guoqi zhu yongjin ZHENG Jianfeng YU Guang NI Xinfeng YAN Lei TIAN Lei HUANG Lili Research Institute of Petroleum Exploration&Development PetroChinaBeijing 100083China PetroChina Hangzhou Research Institute of Geology(HIPG) Hangzhou 310023China Key Laboratory of Carbonate Reservoirs CNPCHangzhou 310023China Research Institute of Petroleum Exploration&Development-Northwest(NWGI) PetroChinaLanzhou 730020China

To predict the large-scale source rock and reservoir distribution in Cambrian subsalt formations(Upper Sinian to Mid-Cambrian)in the Tarim Basin,more than 502D seismic lines and 3D data volume acquired latest,22 wells and some out-crops around this area were used to study the tectonic-lithofacies paleogeography,define the distribution of large scale source rocks and reservoirs,and sort out favorable zones.(1)The basin experienced evolution from ramp to deep-water mud-rich ramp,low-gradient slope,weak rimmed to strong rimmed platform,and the differentiation in the platform was controlled by pre-Cambrian palaeo-rifts.(2)The Luonan-Yubei ancient rift controlled the distribution of source rocks in the Lower Cambrian Yuertusi Formation,and this rift together with the northern depression are the main source rock area.(3)There are three sets of large-scale reservoirs,the Upper Sinian Qigebulake Formation,the Lower Cambrian Xiaorbulake Formation and the Wu-songger Formation,and shoal-mounds,early dolomitization and multi-levels of unconformities controlled reservoir develop-ment.(4)Four favorable zones,the north slope of Tazhong,upper slope of Maigaiti area,Keping-Wensu periphery,and south slope of Tabei,were *** them,the north slope of Tazhong is most likely to have exploration breakthrough;the south slope of Tabei is the best area for exploring the Upper Sinian large scale weathering crust dolomite;Maigaiti slope and Kep-ing-Wensu periphery area improved in exploration potentials significantly,and are worth prospecting faster.

关键词： tectonic-lithofacies paleogeography source-reservoir assemblage favorable zone Late Sinian to Middle Cam-brian Tarim Basin

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PANCONNECTIVITY AND 2-CONNECTED CLAW-FREE GRAPHS

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Systems Science and Mathematical Sciences 1996年第1期9卷 5-12页

作者： GAO Jingzhen(Department of Mathematics, Shaddock Normal University, Jinan 250014,China)zhu yongjin(Institute of Systems Science, Academia Sinica, Beijing 100080,China) 山东师范大学数学系山东济南中科院系统科学所北京

ＰＡＮＣＯＮＮＥＣＴＩＶＩＴＹＡＮＤ２－ＣＯＮＮＥＣＴＥＤＣＬＡＷ－ＦＲＥＥＧＲＡＰＨＳ￥ＧＡＯＪｉｎｇｚｈｅｎ（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｅｍａｔｉｃｓ，ＳｈａｄｄｏｃｋＮｏｒｍａｌＵｎｉｖｅｒｓｉｔｙ，Ｊｉ... 详细信息

ＰＡＮＣＯＮＮＥＣＴＩＶＩＴＹＡＮＤ２－ＣＯＮＮＥＣＴＥＤＣＬＡＷ－ＦＲＥＥＧＲＡＰＨＳ￥ＧＡＯＪｉｎｇｚｈｅｎ（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｅｍａｔｉｃｓ，ＳｈａｄｄｏｃｋＮｏｒｍａｌＵｎｉｖｅｒｓｉｔｙ，Ｊｉｎａｎ２５００１４，Ｃｈｉｎａ）Ｚ...

关键词： Claw-free graphs length of path panconnectivity.

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IMPLICIT DEGREES AND CHVATAL'S CONDITION FOR HAMILTONICITY

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Systems Science and Mathematical Sciences 1989年第4期2卷 353-363页

作者：朱永津高敬振 Institute of Systems Science Academia SinicaBeijingChina

Based on the ideas in[9],an integer d0（v）,called the implicit degree of v whichsatisfies d0（v）≥d（v）,is associated with each vertex v of a graph *** is proved that if theimplicit degree sequence d10,d20,…,dn0（where d10≤d20≤…≤dn0）of a simple graph G on n≥3vertices satisfiesdi0≤in-i0≥n-i,then G is *** is an improvement of the well-known theorem of Chvatal（[4]）.

关键词： Hamiltonian graph implicit degree Chvatal’s condition hamiltonicity preserving supergraph

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