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Multicellular tumor spheroids bridge the gap between two-dimensional cancer cells and solid tumors: The role of lipid metabolism and distribution

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Chinese Chemical Letters 2023年第2期34卷 259-262页

作者： Peisi Xie Jinghui Zhang Pengfei wu yongjiang wu Yanjun Hong Jianing Wang Zongwei Cai State Key Laboratory of Environmental and Biological Analysis Department of ChemistryHong Kong Baptist UniversityHong KongChina College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310058China School of Pharmaceutical Sciences(Shenzhen) Sun Yat-sen UniversityShenzhen 518107China

Previous studies demonstrated that three-dimensional(3D) multicellular tumor spheroids(MCTS) could more closely mimic solid tumors than two-dimensional(2D) cancer cells in terms of the spatial structure, extracellular matrix-cell interaction, and gene expression pattern. However, no study has been reported on the differences in lipid metabolism and distribution among 2D cancer cells, MCTS, and solid tumors. Here, we used Hep G2 liver cancer cell lines to establish these three cancer models. The variations of lipid profiles and spatial distribution among them were explored by using mass spectrometry-based lipidomics and matrix-assisted laser desorption/ionization mass spectrometry imaging(MSI). The results revealed that MCTS, relative to 2D cells, had more shared lipid species with solid tumors. Furthermore,MCTS contained more comparable characteristics than 2D cells to solid tumors with respect to the relative abundance of most lipid classes and mass spectra patterns. MSI data showed that 46 of 71 lipids had similar spatial distribution between solid tumors and MCTS, while lipids in 2D cells had no specific spatial distribution. Interestingly, most of detected lipid species in sphingolipids and glycerolipids preferred locating in the necrotic region to the proliferative region of solid tumors and MCTS. Taken together, our study provides the evidence of lipid metabolism and distribution demonstrating that MCTS are a more suitable in vitro model to mimic solid tumors, which may offer insights into tumor metabolism and microenvironment.

关键词： Lipid metabolism Lipid distribution Two-dimensional cells Three-dimensional cell spheroids Solid tumors

Application of particle swarm optimization-based least square support vector machine in quantitative analysis of extraction solution of yangxinshi tablet using near infrared spectroscopy

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Journal of Innovative Optical Health Sciences 2014年第6期7卷 40-48页

作者： Weijian Lou Kai Yang Miaoqin Zhu yongjiang wu Xuesong Liu Ye Jin Department of Pharmacy Sir Run Run Shaw Hospital of School of Medicine Zhejiang University Hangzhou 310016P.R.China College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058P.R.China Department of Chemistry Zhejiang International Studies University Hangzhou 310012 P.R.China

A particle swarm optimization(PSO)-based least square support vector machine(LS-SVM)method was investigated for quantitative analysis of extraction solution of Y angxinshi tablet using near infrared(NIR)*** usable spectral region(5400-6200cm^(-1))was identified,then the first derivative spectra smoothed using a Savitzky-Golay filter were employed to establish calibration *** PSO algorithm was applied to select the LS-SVM hyper-parameters(including the regularization and kernel parametens).The calibration models of total flavonoids,puerarin,salvianolic acid B and icarin were established using the optimumn hyper-parameters of LS *** performance of LS SVM models were compared with partial least squares(PLS)regression,feed forward back propagation network(BPANN)and support vector machine(SVM).Experimental results showed that both the calibration results and prediction accuracy of the PSO-based LS SVM method were superior to PLS,BP-ANN and *** PSO-based LS-SVM models,the determination cofficients(R2)for the calibration set were above 0.9881,and the RSEP values were controlled within 5.772%.For the validation set,the RMSEP values were close to RMSEC and less than 0.042,the RSEP values were under 8.778%,which were much lower than the PLS,BP-ANN and SVM *** PSO-based LS SVM algorithm employed in this study exhibited excellent calibration performance and prediction accuracy,which has definite practice significance and application value.

关键词： Near infrared spectroscopy extraction paurticle swarm optimization least square support vector machines

Rapid and simultaneous determination of moisture and berberine content in Coptidis Rhizoma and Phellodendri Chinensis Cortex by near-infrared spectroscopy and chemometrics

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Journal of Innovative Optical Health Sciences 2020年第2期13卷 57-69页

作者： Siyu Zhang Ming Chen Yong Chen Wei Xiao Yerui Li Jun Wang Xuesong Liu yongjiang wu Institute of Modern Chinese Medicine College of Pharmaceutical Science Zhejiang University Hangzhou 310058P.R.China Jiangsu Kanion Pharmaceutical Co. Ltd.Lianyungang 222001P.R.China Suzhou ZeDaXingBang Pharmaceutical Co. Ltd.Suzhou 215000P.R.China

Coptidis Rhizoma(Chinese:Huanglian)and Phellodendri Chinensis Cortex(Chinese:Huangbo)are widely used Traditional Chinese Medicine,and often used in combination because of their similar pharmacological effects in clinical ***,the quality control methods of the two drugs are different and complicated,which is time consuming and laborious in practical *** this paper,rapid and simultaneous determination of moisture and berberine in Coptidis Rhizoma(CR)and Phellodendri Chinensis Cortex(PC)was realized by near-infrared spectroscopy(NIRs)combined with global *** adaptive reweighted sampling(CARS)and successive projection algorithm(SPA)method were applied for variable *** component analysis(PCA)and partial least squares regression method(PLSR)were applied for qualitative and quantitative analysis,*** characteristic variables of berberine showed similarity and consistency in distribution,providing basis for the global *** moisture content,the global model had relative standard error of prediction set(RSEP)value of 3.04%and 2.53%for CR and PC,*** berberine content,the global model had RSEP value of 5.41%and 3.97%for CR and PC,*** results indicated the global models based on CARS-PLS method achieved satisfactory prediction for moisture and berberine content,improving the determination e±***,the greater range and larger number of samples enhanced the reliance of the global *** NIRs combined with global models could be a powerful tool for quality control of CR and PC.

关键词： Near-infrared spectroscopy global model Coptidis Rhizoma Phellodendri Chinensis Cortex berberine

铸牢中华民族共同体意识的关系建构、关系原理与关系策略

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西南民族大学学报（人文社会科学版） 2022年第5期43卷 1-8页

作者：武永江长安大学地球科学与资源学院陕西西安710054

铸牢中华民族共同体意识离不开和谐的民族关系。基于关系建构主义视阈,铸牢中华民族共同体意识就是要建构各民族的共生关系、利益关系、合作关系和情感关系。同时,需要遵循关系原理:铸牢中华民族共同体意识是一种治理关系选择的过程、... 详细信息

铸牢中华民族共同体意识离不开和谐的民族关系。基于关系建构主义视阈,铸牢中华民族共同体意识就是要建构各民族的共生关系、利益关系、合作关系和情感关系。同时,需要遵循关系原理:铸牢中华民族共同体意识是一种治理关系选择的过程、民主关系文化的展现、身份关系语境的建构和分歧关系视角的理解。为此,铸牢中华民族共同体意识需要以考虑他人想法来提升民族认知、以沟通交流达成民族共识、以民主素质扩展民族包容、以担当意识促进民族协同和以共情能力建构民族信任。铸牢中华民族共同体意识的关系路径将创新民族团结进步教育的理念与举措。

关键词：铸牢中华民族共同体意识关系学民族团结

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论导师与研究生非指导型谈话的构建

中国高教研究 2010年第5期 33-35页

作者：武永江陕西师范大学政治经济学院长安大学资源学院讲师陕西西安710062

导师经常通过交谈与研究生进行沟通,进而实施教育。在导师与研究生的谈话中,除了常见的"指导型谈话"外,还应构建一种"非指导型谈话",以体现"学生为中心"的教育思想。"非指导型谈话"具有自身的特点和做法,但需要避免一些误区,以满足研究... 详细信息

导师经常通过交谈与研究生进行沟通,进而实施教育。在导师与研究生的谈话中,除了常见的"指导型谈话"外,还应构建一种"非指导型谈话",以体现"学生为中心"的教育思想。"非指导型谈话"具有自身的特点和做法,但需要避免一些误区,以满足研究生发展的需要。

关键词：导师谈话法非指导和谐

中药材三七中皂苷类成分的近红外光谱快速无损分析新方法

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化学学报 2003年第3期61卷 393-398页

作者：杨南林程翼宇吴永江浙江大学化学工程与生物工程学系杭州310027

提出了用近红外漫反射光谱快速无损测定三七中皂苷类成分的新方法 .采用HPLC分析了中药材三七固粉中三七皂苷R1,人参皂苷Rg1,Rb1和Rd的含量 ,用吸附树脂比色法测定了三七总皂苷 (PNS)的含量 ,共获得三七固粉样本 39个 ,R1,Rg1,Rb1,Rd ,... 详细信息

提出了用近红外漫反射光谱快速无损测定三七中皂苷类成分的新方法 .采用HPLC分析了中药材三七固粉中三七皂苷R1,人参皂苷Rg1,Rb1和Rd的含量 ,用吸附树脂比色法测定了三七总皂苷 (PNS)的含量 ,共获得三七固粉样本 39个 ,R1,Rg1,Rb1,Rd ,PNS的含量范围分别为 1 5 8～ 5 0 8,2 1 68～ 46 13,11 46～ 40 41,3 38～ 8 87,64 2 2～ 14 5 13mg·g-1药粉 .在 35 0 0～ 110 0 0cm-1扫描样品 ,以交叉验证误差均方根 (RMSECV)为指标 ,通过筛选 ,对各组分确定了用于建模的最优近红外波段和光谱预处理方法 .采用偏最小二乘算法建立了近红外光谱与 5个组分HPLC分析值之间的校正模型 ,并以此预测了 8个未知样本 .R1,Rg1,Rb1,Rd及PNS校正模型的RMSECV分别为 0 40 ,1 47,1 94,0 5 3,5 15 ,预测误差均方根RMSEP分别为 0 5 3,3 15 ,2 14 ,0 70 ,9 0 3.该方法快速无损 ,结果可靠。

关键词：皂苷无损分析近红外漫反射光谱偏最小二乘中药材分析三七多元校正

“问题学生”教育转化的新思路——基于“问题学生”的主观框架理念

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中国特殊教育 2006年第11期 30-33页

作者：武永江长安大学地球科学与国土资源学院西安710054

“问题学生”是学校教育不可忽视的重点对象,从心理咨询与治疗的一些视角来认识“问题学生”,会有一些新的认识:“问题”是学生人生发展所必需的,“问题学生”的标准是发展的,“问题学生”自身也是有潜力的,“问题学生”有自己独特的主... 详细信息

“问题学生”是学校教育不可忽视的重点对象,从心理咨询与治疗的一些视角来认识“问题学生”,会有一些新的认识:“问题”是学生人生发展所必需的,“问题学生”的标准是发展的,“问题学生”自身也是有潜力的,“问题学生”有自己独特的主观世界。而在传统的基于已有“理论假设”而进行的教育转化也存在一定的不足:“理论假设”是理解普遍性问题的;“理论假设”实施者的过分效忠;改变学生,以适应已有理论假设。基于“问题学生”主观框架的转化思路为:调整转化过程,以适应问题学生的资源;调适转化关系;调适转化,使之适应问题学生的改变理论。

关键词：问题学生主观框架转化发展