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百香果(Passiflora edulis)R2R3-MYB基因家族的结构和功能分析

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果树学报 2024年第1期41卷 12-29页

作者：张丹郑平邓彪柴改凤安昌邓考张文斌陆宇明卢翔宇王小媚秦源福建农林大学生命科学学院福州350002 广西壮族自治区农业科学院园艺研究所南宁530007 福建农林大学海峡联合研究院福州350000 龙岩市新罗区良种繁育场福建龙岩364000

【目的】探究R2R3-MYB转录因子在代谢、发育等多种生物学过程中发挥的调控作用。对百香果R2R3-MYB基因家族进行表达分析并探究其在花果发育过程中的调控机制,对深入研究R2R3-MYB转录因子在百香果中的生物学作用中具有重要意义。【方法... 详细信息

【目的】探究R2R3-MYB转录因子在代谢、发育等多种生物学过程中发挥的调控作用。对百香果R2R3-MYB基因家族进行表达分析并探究其在花果发育过程中的调控机制,对深入研究R2R3-MYB转录因子在百香果中的生物学作用中具有重要意义。【方法】基于百香果全基因组数据,通过HMM搜索对R2R3-MYB家族成员进行筛选鉴定,并利用实时荧光定量PCR分析其在百香果花不同组织中的表达模式。【结果】从百香果基因组中鉴定出99个R2R3-MYB基因,根据系统进化分析将其分为36个亚组。99个R2R3-PeMYB基因随机分布在9条百香果染色体上。基序和基因结构分析表明,R2R3-PeMYB在同一亚组中具有相对保守的结构特征。共线性分析表明,片段复制事件促进了百香果R2R3-MYB家族的扩张。基于转录组数据和qRT-PCR分析,发现R2R3-PeMYB基因在百香果花的不同组织的不同发育阶段中表现出差异的表达模式,说明R2R3-PeMYB基因在花不同组织和果实的发育过程中发挥重要作用,尤其是PeMYB62和PeMYB63在百香果果皮转色期的高表达,说明其可能参与百香果花青素的合成。同时8个R2R3-PeMYB基因在非生物胁迫(冷、热、盐和渗透压)下也表现出不同的反应,说明R2R3-PeMYB基因还受到非生物胁迫的诱导。【结论】鉴定了99个R2R3-PeMYB基因家族成员,发现其在百香果花果发育和胁迫诱导方面发挥重要作用,为今后研究百香果R2R3-MYB基因在花果发育中的功能和进化提供了有价值的线索。

关键词：百香果 R2R3-MYB转录因子系统发育分析花果发育

Applying a Step Approach Method in Solving the Multi-Frequency Radiation From a Complex Obstacle

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Computer Modeling in Engineering & Sciences 2016年第7期112卷 59-73页

作者： Jui-Hsiang kao Department of Systems Engineering and Naval Architecture National Taiwan Ocean UniversityKeelungTaiwanR.O.C

In this paper,a step approach method in the time domain is developed to calculate the radiated waves from an arbitrary obstacle pulsating with multiple *** computing scheme is based on the Boundary Integral Equation and derived in the time domain;thus,the time-harmonic Neumann boundary condition can be *** the present method,the values of the initial conditions are set to zero,and the approach process is carried forward in a loop from the first time step to the *** each time step,the radiated pressure on each element is *** several loops,the correct radiated pressures can be obtained.A sphere pulsating with a monopole frequency in an infinite acoustic domain is calculated *** result is compared with the analytical solution,and both of them are in good ***,a complex-shaped radiator is taken as the studied *** pulsating frequency of this case is multiple,and the waves propagate in half *** is shown that the present method can treat multiple-frequency pulsation well,even when the radiator is a complex shape,and a robust convergence can be attained quickly.

关键词： Step approach method Time domain Radiated waves Multiple frequencies Boundary integral equation Neumann boundary condition

HIV-1 Subtype Diversity and Factors Affecting Drug Resistance among Patients with Virologic Failure in Antiretroviral Therapy in Hainan Province,China,2014–2020

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生物医学与环境科学:英文版 2023年第9期36卷 800-813页

作者： YU De E XU Yu Jun LI Mu YANG Yuan LIANG Hua Yue ZHONG Shan Mei QIN Cai LAN Ya Nan LI Da Wei YU Ji Peng PANG Yuan QIN Xue Qiu LIANG Hao ZHU kao kao YE Li LIANG Bing Yu Guangxi Key Laboratory of AIDS Prevention and Treatment School of Public HealthGuangxi Medical UniversityNanning 530021GuangxiChina International School of Public Health and One Health Hainan Medical UniversityHaikou 571199HainanChina Prevention and Treatment Department the Fifth People's Hospital of Hainan Province&Affiliated Dermatology Hospital of Hainan Medical UniversityHaikou 570102HainanChina Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry Life Sciences InstituteGuangxi Medical UniversityNanning 530021GuangxiChina Oncology Medical College Guangxi Medical UniversityNanning 530021GuangxiChina The First Clinical Medical College Guangxi Medical UniversityNanning 530021GuangxiChinaGuangxi Key Laboratory of AIDS Prevention and TreatmentSchool of Public HealthGuangxi Medical UniversityNanning 530021GuangxiChina

Objective This study aimed to determine the HIV-1 subtype distribution and HIV drug resistance(HIVDR)in patients with ART failure from 2014 to 2020 in Hainan,*** A 7-year cross-sectional study was conducted among HIV/AIDS patients with ART failure in *** used online subtyping tools and the maximum likelihood phylogenetic tree to confirm the HIV subtypes with pol *** resistance mutations(DRMs)were analyzed using the Stanford University HIV Drug Resistance *** A total of 307 HIV-infected patients with ART failure were included,and 241 available pol sequences were *** 241 patients,CRF01_AE accounted for 68.88%,followed by CRF07_BC(17.00%)and eight other subtypes(14.12%).The overall prevalence of HIVDR was 61.41%,and the HIVDR against non-nucleoside reverse transcriptase inhibitors(NNRTIs),nucleotide reverse transcriptase inhibitors(NRTIs),and protease inhibitors(PIs)were 59.75%,45.64%,and 2.49%,*** patients,hypoimmunity or opportunistic infections in individuals,and samples from 2017 to 2020 increased the odd ratios of ***,HIVDR was less likely to affect female *** common DRMs to NNRTIs were K103N(21.99%)and Y181C(20.33%),and M184V(28.21%)and K65R(19.09%)were the main DRMs against *** The present study highlights the HIV-1 subtype diversity in Hainan and the importance of HIVDR surveillance over a long period.

关键词： HIV-1 subtypes Antiretroviral therapy Virological failure Drug resistance

An Improved Training Algorithm Based on Ensemble Penalized Cox Regression for Predicting Absolute Cancer Risk

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China CDC weekly 2023年第9期5卷 206-212,I0009-I0014页

作者： Liyuan Liu Fu Yang Yeye Fan Chunyu kao Fei Wang Lixiang Yu Yong He Jiadong Ji Zhigang Yu Department of Breast Surgery The Second HospitalCheeloo College of MedicineShandong UniversityJinan CityShandong ProvinceChina School of Mathematics Shandong UniversityJinan CityShandong ProvinceChina Zhongtai Securities Institute for Financial Studies Shandong UniversityJinan CityShandong ProvinceChina Institute of Translational Medicine of Breast Disease Prevention and Treatment Shandong UniversityJinan CityShandong ProvinceChina.

Introduction:Biases in cancer incidence characteristics have led to significant imbalances in databases constructed by prospective cohort *** they use imbalanced databases,many traditional algorithms for training cancer risk prediction models perform ***:To improve prediction performance,we introduced a Bagging ensemble framework to an absolute risk model based on ensemble penalized Cox regression(EPCR).We then tested whether the EPCR model outperformed other traditional regression models by varying the censoring rate of the simulated ***:Six different simulation studies were performed with 100 *** assess model performance,we calculated mean false discovery rate,false omission rate,true positive rate,true negative rate,and the areas under the receiver operating characteristic curve(AUC)*** found that the EPCR procedure could reduce the false discovery rate(FDR)for important variables at the same true positive rate(TPR),thereby achieving more accurate variable *** addition,we used the EPCR procedure to build a breast cancer risk prediction model based on the Breast Cancer Cohort Study in Chinese Women *** for 3-and 5-year predictions were 0.691 and 0.642,representing improvements of 0.189 and 0.117 over the classical Gail model,***:We conclude that the EPCR procedure can overcome challenges posed by imbalanced data and improve the performance of cancer risk assessment tools.

关键词： Cancer false prediction

八车道高速公路交通轴载与沥青路面使用性能指标的车道差异性

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公路 2024年第5期69卷 424-432页

作者：黄考取陈宇徐文军郝金海晏博智周献龙朱传江谷雨浙江交工集团股份有限公司设计院分公司公路数智养护浙江省工程研究中心杭州市310051 浙江沪杭甬高速公路股份有限公司宁波管理中心宁波市315016 浙江师范大学浙江省城市轨道交通智能运维技术与装备重点实验室金华市321004

为积极响应高速公路集约化施工的思想,实现高速公路车道级精准养护,以浙江省沪杭甬公路典型八车道路段交通轴载和沥青路面使用性能指标数据为基础,分析了其交通轴载的车道差异性,并采用多种拟合模型和集度分析相结合的方法,分析了历年... 详细信息

为积极响应高速公路集约化施工的思想,实现高速公路车道级精准养护,以浙江省沪杭甬公路典型八车道路段交通轴载和沥青路面使用性能指标数据为基础,分析了其交通轴载的车道差异性,并采用多种拟合模型和集度分析相结合的方法,分析了历年车辙深度RD、国际平整度指数IRI和路面破损率DR等3项使用性能指标的车道差异性,探讨了交通轴载与各使用性能指标的相关性及其车道差异性。结果表明:交通轴载和沥青路面各使用性能指标及其集度均与所在车道有关,各严重病害主要集中在第三、四车道,交通轴载与各使用性能指标的相关性分析结果在第一、二车道和第三、四车道也分别呈现不同的团聚。因此,建议在后续双向八车道高速公路养护工作中,充分考虑第一、二车道和第三、四车道的差异性。

关键词：沥青路面精准养护决策车道系数车辙深度国际平整度指数路面破损率集度拟合分析

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Numerical Simulation of Co-Combustion of Pulverized Coal and Different Proportions of Refused Derived Fuel in TTF Precalciner

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Journal of Renewable Materials 2021年第7期9卷 1329-1343页

作者： Jiekun Zhu Hongtao kao College of Materials Science and Engineering Nanjing Tech UniversityNanjing211816China

Based on the theory of computational fluid dynamics(CFD),pulverized coal combustion alone,and the co-combustion of pulverized coal and refuse-derived fuel(RDF)in a Trinal-sprayed calciner(TTF)precalciner were *** results revealed that when coal was used as a single fuel,the velocity field in the precalciner had good symmetry,and formed three spray effects and multiple recirculation *** main combustion zone was distributed in the lower tertiary air and pulverized coal area,and the highest temperature reached up to 1,500 *** to the simulation results,the predicted decomposing rate of raw meal was 90.12%,which is in good agreement with the actual measured *** addition,with the increase in RDF content,the average temperature of the furnace,the decomposition rate of the raw meal,and the NO_(x) concentration all exhibited a downward *** the condition of ensuring the normal operation of the precalciner,blending with 20%RDF is the most reasonable strategy,and the NO_(x) emissions decreased by approximately 16%.

关键词： Numerical simulation TTF precalciner RDF co-combustion NO_(x)emission

Numerical Simulation of Sludge Combustion in TTF Precalciner

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Journal of Renewable Materials 2021年第4期9卷 671-693页

作者： Penghui Wang Hongtao kao College of Materials Science and Engineering Nanjing Tech UniversityNanjing211816China

The research method of computational fluid dynamics(CFD)is used to study the technology of burning sludge in cement *** simulation results show that the flow field in the TTF precalciner is stable,the spray effect is good,and no raw meal collapse *** special structure of the TTF(Trinal-sprayed)precalciner allows the pulverized coal and sludge to fully exchange heat with the flue gas and *** there is no sludge burning in the furnace,the decomposition rate can reach 92.1%,and the maximum temperature of the precalciner can reach 1600 *** the moisture content of the sludge is constant,as the amount of sludge increases,the overall furnace temperature shows a downward trend and the minimum temperature at the precalciner outlet can be reduced to 1080 *** phenomenon shows that sludge has a great influence on furnace *** order to ensure that the decomposition rate of the raw meal is not less than 85%,the decomposition rate of the raw meal under different conditions is *** was found that the sludge input can be about S-20%when the mositure content of the sludge is 50%.Moreover,when the water content of the sludge is 60%,the burning amount of sludge cannot be higher than S-20%,which ensures the normal decomposition of *** addition of sludge reduces the temperature in the precalciner and further inhibits the generation of thermal NO_(x).The reduction of CO content produced by incomplete combustion of pulverized coal indicates that CO suppresses the production of fuel-type NO_(x).The NO_(x) concentration at the gooseneck is as low as 522 mg/***,it was also found that when the sludge input is fixed,the NO_(x) concentration decreases with the moisture content of the sludge increasing,indirectly proving the inhibitory effect of H2O on NO_(x).

关键词： Sludge combustion numerical simulation TTF precalciner NO_(x)reduction

Numerical Simulation of Urea Based SNCR Process in a Trinal-Sprayed Precalciner

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Journal of Renewable Materials 2021年第2期9卷 269-294页

作者： Yu Liu Hongtao kao College of Materials Science and Engineering Nanjing Tech UniversityNanjing211816China

In order to study the combustion characteristics,NOx emission and NH3 slip in a new trinal-sprayed precalciner,the simulations of combustion and aqueous urea solution based selective non-catalytic reduction(SNCR)process were conducted by computational fluid dynamics in this precalciner,the effects of different injection heights,different injection flow rates and stratified injection under different flow rates on SNCR process were *** results showed that the flow field was symmetrically distributed in the precalciner,and the flue gas from the rotary kiln formed the recirculation region on both sides of the cone body,which increased the residence time of the solid *** temperature was mainly between 1100 K and 1250 K in the middle and upper column of the precalciner,which met the demand of the pulverized coal combustion and raw material *** concentration of NO at the outlet of the precalciner was 559 ppm,moreover,different injection heights and different injection flow rates had a strong influence on NOX removal efficiency and NH3 *** aqueous urea solution should be injected at SNCR-1 to prolong the residence time of NH3,and injection flow rate had an optimal flow rate but not the higher the *** the injection flow rate under stratified injection was 0.019 kg/s,which could play a better optimization role on NO removal efficiency on the basic of the injection flow *** consideration of cost effective,a stratified injection with an injection flow rate of 0.019 kg/s and an injection height of 20 m,25 m and 30 m was suggested as a compromise of a satisfactory NOx reduction rates and reasonable NH3 *** this condition,numerical simulation result showed that NOx concentration at the outlet of precalciner was 297.27 mg/Nm3 and NH3 slip was 4.67 mg/Nm3,meeting emission standard.

关键词： Trinal-sprayed precalciner selective non-catalytic reduction aqueous urea solution NOx reduction

Generalized Artificial Life Structure for Time-dependent Problems

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Chinese Journal of Mechanical Engineering 2009年第3期22卷 317-324页

作者： TSAU Minhe kao Weiwen CHANG Albert Department of Mechanical Engineering Taiwan University of Science and Technology Department of Industrial Management Vanung University of Science and Technology

In recent years, more attention has been paid on artificial life researches. Artificial life（AL） is a research on regulating gene parameters of digital organisms under complicated problematic environments through natural selections and evolutions to achieve the final emergence of intelligence. Most recent studies focused on solving certain real problems by artificial life methods, yet without much address on the AL life basic mechanism. The real problems are often very complicated, and the proposed methods sometimes seem too simple to handle those problems. This study proposed a new approach in AL research, named ＂generalized artificial life structure（GALS）＂, in which the traditional ＂gene bits＂ in genetic algorithms is first replaced by ＂gene parameters＂, which could appear anywhere in GALS. A modeling procedure is taken to normalize the input data, and AL ＂tissue＂ is innovated to make AL more complex. GALS is anticipated to contribute significantly to the fitness of AL evolution. The formation of ＂tissue＂ begins with some different AL basic cells, and then tissue is produced by the casual selections of one or several of these cells. As a result, the gene parameters, represented by ＂tissues＂, could become highly diversified. This diversification should have obvious effects on improving gene fitness. This study took the innovative method of GALS in a stock forecasting problem under a carefully designed manipulating platform. And the researching results verify that the GALS is successful in improving the gene evolution fitness.

关键词： artificial life artificial intelligence generalized artificial life structure (GALS)