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Synthesis of Polyethylenes with In-Chain Isolated Carbonyls from CO_(2)and Ethylene via a Tandem Photoreduction/Polymerization Protocol

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CCS Chemistry 2024年第6期6卷 1591-1599页

作者： Fulin Zhou Haojie Dai Shan Tang yongfeng Zhou Frontiers science center for Transformative Molecules Shanghai Key Laboratory for Molecular Engineering of Chiral DrugsSchool of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240

Aiming at accessing polyethylene-bearing in-chain degradable functionality,copolymerization of carbon dioxide(CO_(2))with ethylene has long been recognized as a significant but highly challenging ***,most trials have resulted in ethylene homopolymerization because of the endothermic property and high energy barriers of CO_(2)insertion during polymer chain *** we report an unprecedented tandem reaction protocol for the synthesis of polyethylenes with in-chain carbonyls from CO_(2)and ethylene by the combination of photocatalytic CO_(2)reduction and palladium-catalyzed coordination/insertion *** protocol provides an easy way to adjust the carbonyl content(from 0.13 to 12 mol%)and insertion selectivity(up to 99%isolated carbonyls)of the polyethylene copolymers,which enables the polymers tomaintain the main properties of highdensity polyethylene and simultaneously endows them with good *** addition,besides apolar polyethylene copolymers,polar-functionalized polyethylenes with in-chain isolated carbonyls can also be synthesized from CO_(2),ethylene and a polar monomer using the same *** believe our present work sheds new light on the synthesis of degradable polyethylenes by employing CO_(2)as a green carbonyl source.

关键词： carbon dioxide coordination polymerization polyethylene photoreduction photodegradation

Ryanodine receptor-protein regulator interaction revealed a general molecular mechanism of channel inhibition

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Ryanodine receptor-protein regulator interaction revealed a ...

Ryanodine receptor-protein regulator interaction revealed a general molecular mechanism of channel inhibition

第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： Chang-Cheng Yin department of Biophysics,health science center,peking university,38 xueyuan Road,beijing 100191

＜正＞Ryanodine receptors（RyR）are the major Ca2+release channels in both cardiac and skeletalmuscle,they play a crucial role in the Ca2+signaling pathway that govern the muscle

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生物物理学报 2009年第S1期25卷 79-79页

作者： Chang-Cheng Yin department of Biophysics, health science center, peking university, 38 xueyuan Road, beijing 100191,

Ryanodine receptors (RyR) are the major Ca2+ release channels in both cardiac and skeletal muscle, they play a crucial role in the Ca2+ signaling pathway that govern the

关键词： RyR Ryanodine receptor-protein regulator interaction revealed a general molecular mechanism of channel inhibition

Expression,purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells

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World Journal of Gastroenterology 2003年第4期9卷 678-682页

作者： Bing Li Hong-Yan Wu Xiao-Ping Qian Yan Li Wei-Feng Chen Immunology department of peking university health science center,beijing,100083,china Immunology department of peking university health science center 38 Xueyuan Road Beijing 100083 China. wfchen@***

AIM: In order to assess hepatocellular carcinoma associated antigen HCA587 as a potential target for immunotherapy,the Bac-to-Bac expression system was used to express recombinant protein HCA587 in insect ***: The cDNA encoding HCA587 gene was cloned into donor vector pFasBacHtb and recombinant pFasBac Htb587 was transformed into competent cells *** Bacmid-587 was transfected into Sf9 insect cells using CELLFECTIN, Recombinant HCA587 protein was produced in Sf9 insect cells after infection with recombinant baculovirus, and was purified using Ni-NTA resin. Sera from HCC patients were also screened using recombinant protein ***: The molecular weight of the recombinant protein HCA587 expressed in insect cells was approximately *** blot results proved the recombinant protein HCA587had the similar antigenicity with its native *** analysis told that the rate of seroreactivity to HCA587 was not high in HCC ***: The recombinant protein HCA587 was successfully expressed and purified using Bac-to-Bac expression system. It paved the way for generation of specific antibody and investigation of immunohistochemical analysis and immune responses of HCC in the future.

关键词： HCA587抗原肝细胞癌血清学检查免疫疗法免疫组织化学

Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

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science china(Life sciences) 2009年第9期52卷 885-890页

作者： SHI yongfeng1,2,CHEN Jie1,LIU WenQiang1,HUANG QiNa1,2,SHEN Bo2,LEUNG Hei3 & WU JianLi1 1 Chinese National center for Rice Improvement/National key Laboratory for Rice biology,china National Rice Research Institute,Hangzhou 310006,china 2 College of Life and Environmental sciences,Hangzhou Normal university,Hangzhou 310016,china 3 International Rice Research Institute,Metro Manila DAPO Box 7777,Philippines Chinese National center for Rice Improvement/National key Laboratory for Rice biology China National Rice Research Institute Hangzhou China College of Life and Environmental sciences Hangzhou Normal University Hangzhou China International Rice Research Institute Manila Philippines

To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway.

关键词： rice (Oryza sativa L.) chemical mutagenesis molecular marker rolled-leaf gene physical map

ASF1A-dependent P300-mediated histone H3 lysine 18 lactylation promotes atherosclerosis by regulating EndMT

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Acta Pharmaceutica Sinica B 2024年第7期14卷 3027-3048页

作者： Mengdie Dong Yunjia Zhang Minghong Chen Yongkang Tan Jiao Min Xian He Fuhao Liu Jiaming Gu Hong Jiang Longbin Zheng Jiajing Chen Quanwen Yin Xuesong Li Xiang Chen yongfeng Shao Yong Ji Hongshan Chen department of Cardiovascular Surgery the First Affiliated Hospital of Nanjing Medical Universityand Key Laboratory of Cardiovascular and Cerebrovascular MedicineSchool of PharmacyNational Vaccine Innovation PlatformNanjing Medical UniversityNanjing 211166China Key Laboratory of Cardiovascular and Cerebrovascular Medicine School of PharmacyNanjing Medical UniversityNanjing 211166China department of Clinical Medicine Nanjing Medical University TIANYUAN Honors SchoolNanjing Medical UniversityNanjing 211166China department of Anesthesiology Sir Run Run HospitalNanjing Medical UniversityNanjing 211166China department of Cardiovascular Surgery the First Affiliated Hospital of Nanjing Medical UniversityNanjing 211166China Key Laboratory of Cardiovascular and Cerebrovascular Medicine Key Laboratory of Targeted Intervention of Cardiovascular DiseaseCollaborative Innovation Center for Cardiovascular Disease Translational MedicineState Key Laboratory of Reproductive MedicineSchool of Pharmacythe Affiliated Suzhou Hospital of Nanjing Medical UniversityGusu SchoolNanjing Medical UniversityNanjing 211166China National Key Laboratory of Frigid Zone Cardiovascular Diseases(NKLFZCD) Department of Pharmacology(State-Province Key Laboratories of Biomedicine-Pharmaceutics of China)College of PharmacyKey Laboratory of Cardiovascular Medicine Research and Key Laboratory of Myocardial IschemiaChinese Ministry of EducationNHC Key Laboratory of Cell Transplantationthe Central Laboratory of the First Affiliated HospitalHarbin Medical UniversityHarbin 150081China Key Laboratory of Targeted Intervention of Cardiovascular Disease Collaborative Innovation Center for Cardiovascular Disease Translational MedicineNanjing Medical UniversityNanjing 211166China department of Cardiology Huai’an First People’s Hospital Affiliated with Nanjing Medical UniversityHuai’an 223399China

Endothelial-to-mesenchymal transition(EndMT)is a key driver of *** glycolysis is increased in the endothelium of atheroprone areas,accompanied by elevated lactate *** lactylation,mediated by lactate,can regulate gene expression and participate in disease ***,whether histone lactylation is involved in atherosclerosis remains ***,we report that lipid peroxidation could lead to EndMT-induced atherosclerosis by increasing lactatedependent histone H3 lysine 18 lactylation(H3K18la)in vitro and in vivo,as well as in atherosclerotic patients’***,the histone chaperone ASF1A was first identified as a cofactor of P300,which precisely regulated the enrichment of H3K18la at the promoter of SNAI1,thereby activating SNAI1 transcription and promoting *** found that deletion of ASF1A inhibited EndMT and improved endothelial *** analysis based on Apoe^(KO)Asf1a^(ECKO) mice in the atherosclerosis model confirmed the involvement of H3K18la in atherosclerosis and found that endotheliumspecific ASF1A deficiency inhibited EndMT and alleviated atherosclerosis *** of glycolysis by pharmacologic inhibition and advanced PROTAC attenuated H3K18la,SNAI1 transcription,and EndMT-induced *** study illustrates precise crosstalk between metabolism and epigenetics via H3K18la by the P300/ASF1A molecular complex during EndMT-induced atherogenesis,which provides emerging therapies for atherosclerosis.

关键词： Histone lactylation Atherosclerosis Endothelial-tomesenchymal transition ASF1A SNAI1 Endothelial dysfunction Lactate Epigenetic

Epigenetics and cancer

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Epigenetics and cancer

LSD1 is required for euchromatic origin firing and replication timing

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： yongfeng shang department of biochemistry and molecular biology peking university health science center

Epigenetic mechanisms are essential for normal development and maintenance of lineage-specific gene expression patterns in *** of epigenetic processes can lead to the alteration of gene function and malignant transformation. The initiation and progression of cancer,traditionally seen as a genetic disease,is now realized to involve epigenetic abnormalities along with genetic ***

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Signal Transduction and Targeted Therapy 2022年第5期7卷 1703-1718页

作者： Yue Wang Yunchao Huang Edith Cheng Xinhua Liu Yu Zhang Jianguo Yang Jordan T.F.Young Grant W.Brown Xiaohan Yang yongfeng shang department of biochemistry and molecular biology School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China department of biochemistry and molecular biology School of Basic Medical SciencesHangzhou Normal UniversityHangzhou 311121China department of biochemistry and molecular biology School of Basic Medical SciencesCapital Medical UniversityBeijing 100069China department of biochemistry and Donnelly Centre University of TorontoTorontoON M5S 1A8Canada Lunenfeld-Tanenbaum Research Institute Mount Sinai Hospital600 University Ave.TorontoON M5G 1×5Canada

The chromatin-based rule governing the selection and activation of replication origins remains to be *** is believed that DNA replication initiates from open chromatin domains;thus,replication origins reside in open and active ***,we report here that lysine-specific demethylase 1(LSD1),which biochemically catalyzes H3K4me1/2 demethylation favoring chromatin condensation,interacts with the DNA replication machinery in human *** find that LSD1 level peaks in early S phase.

关键词： origin LSD1 replication

Chromodomain protein CDYL is required for transmission/restoration of repressive histone marks

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Journal of molecular Cell biology 2017年第3期9卷 178-194页

作者： Yongqing Liu Shumeng Liu Shuai Yuan Huajing Yu Yu Zhang Xiaohan Yang Guojia Xie Zhe Chen Wanjin Li Bosen Xu Luyang Sun yongfeng shang Jing Liang Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China department of biochemistry and molecular biology School of Basic Medical Sciences Capital Medical University Beijing 100069 China Tianjin Key Laboratory of Medical Epigenetics Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Tianjin Medical University Tianjin 300070 China

Faithful transmission or restoration of epigenetic information such as repressive histone modifications through generations is crit- ical for the maintenance of cell identity. We report here that chromodomain Y-like protein （CDYL）, a chromodomain-containing transcription corepressor, is physically associated with chromatin assembly factor 1 （CAF-1） and the repiicative heUcase MCM complex. We showed that CDYL bridges CAF-1 and MCM, facilitating histone transfer and deposition during DNA replication. We demonstrated that CDYI. recruits histone-modifying enzymes G9a, SETDB1, and EZH2 to replication forks, leading to the addition of H3Kgme2/3 and H3K27me2/3 on newly deposited histone H3. Significantly, depletion of CDYL impedes early S phase progres- sion and sensitizes cells to DNA damage. Our data indicate that CDYL plays an important role in the transmission/restoration of repressive histone marks, thereby preserving the epigenetic landscape for the maintenance of cell identity.

关键词： epigenetic inheritance histone modification CDYL CAF-1 MCM