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A high-strength mineralized collagen bone scaffold for large-sized cranial bone defect repair in sheep

Regenerative Biomaterials 2018年第5期5卷 283-292页

作者： Shuo Wang Zhijun Zhao yongdong yang Antonios G.Mikos Zhiye Qiu Tianxi Song Fuzhai Cui XiumeiWang Chunyang Zhang State Key Laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced Materials of Ministry of EducationSchool of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Department of Neurosurgery The First Affiliated Hospital of Baotou Medical SchoolBaotou 014010China Dongzhimen Hospital Affiliated Beijing University of Chinese Medicine Beijing 100700China Department of Bioengineering Rice UniversityHoustonTX 77030USA Beijing Allgens Medical Science and Technology Co. Ltd.Beijing 100176China

Large-sized cranial bone defect repair presents a great challenge in the *** ideal cranioplasty materials to realize the functional and cosmetic recovery of the defect must have sufficient mechanical support,excellent biocompatibility,good osseointegration and biodegradability as *** this study,a high-strength mineralized collagen(MC)bone scaffold was developed with biomimetic composition,microstructure and mechanical properties for the repair of sheep largesized cranial bone defects in comparison with two traditional cranioplasty materials,polymethyl methacrylate and titanium *** compact MC scaffold showed no distinct pore structure and therefore possessed good mechanical *** strength and elastic modulus of the scaffold were much higher than those of natural cancellous bone and slightly lower than those of natural compact *** vitro cytocompatibility evaluation revealed that the human bone marrow mesenchymal stem cells(hBMSC)had good viability,attachment and proliferation on the compact MC scaffold indicating its excellent *** adult sheep cranial bone defect model was constructed to evaluate the performances of these cranioplasty materials in repairing the cranial bone *** results were investigated by gross observation,computed tomography scanning as well as histological *** in vivo evaluations indicated that compact MC scaffold showed notable osteoconductivity and osseointegration with surrounding cranial bone tissues by promoting bone *** results suggested that the compact MC scaffold has a promising potential for large-sized cranial bone defect repair.

关键词： mineralized collagen cranial bone defect bone regeneration sheep cranioplasty materials

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Deep electrical structures of Qinzhou-Fangcheng Junction Zone in Guangxi and seismogenic environment of the 1936 Lingshan M6^(3/4)earthquake

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Science China Earth Sciences 2024年第2期67卷 584-603页

作者： Bin ZHOU Chunheng YAN Yan ZHAN Xiangyu SUN Sha LI Xiang WEN Yajun MO yongdong YUAN Yuan YUAN Meili HUANG Earthquake Agency of Guangxi Zhuang Autonomous Region Nanning530022China State Key Laboratory of Earthquake Dynamics Institute of GeologyChina Earthquake AdministrationBeijing100029China Guangxi Geophysical Survey Institute Liuzhou545005China

The tectonic position of the southwest section of the Qinzhou Bay-Hangzhou Bay Tectonic Junction Zone(QHTJZ)can be determined by examining the Qinzhou-Fangcheng Junction Zone(QFJZ)in *** zone is significant because it was the location of the largest earthquake ever recorded in the inland region of South China,specifically the 1936 Lingshan M6^(3/4)earthquake in ***,this region serves as an optimal location for researching the origins of intraplate earthquakes in South *** study presents a display of a broadband magnetotelluric(MT)prospecting profile that traverses the Guangxi QFJZ and the Lingshan earthquake zone,extending from the northwest(NW)to the southeast(SE).A resistivity structure model was generated using three-dimensional(3D)inversion technology along the *** main faults in QFJZ were analyzed in terms of their deep extension forms and tectonic *** analysis was performed by integrating the results obtained from geology,gravity,wave velocity ratio,Global Position System(GPS),and geothermal *** results showed that(1)the Dongzhong-Xiaodong fault(DXf),the eastern Fangcheng-Lingshan fault(FLf2),and the eastern Hepu-Beiliu fault(HBf2)were all trans-crustal deep faults,and crust-mantle ductile shear zones developed in the deep *** electrical boundary zones,DXf and HBf2,were *** inclined towards the northwest,while HBf2 inclined towards the *** FangchengLingshan fault(FLf)exhibits a tectonic style resembling a“flower”shape in the upper *** the deeper section,it is characterized by an electrical boundary zone that gradually slopes towards the southeast direction.(2)The Hunan-Guangxi Passive Continental Margin(HGPCM)on the NW side of DXf had a stratified resistivity structure and relatively stable Bouguer gravity anomalies,which conformed to the quasi-craton tectonic attribute of the local failure at the southeastern margin of the yangtze Block(YB).The southeastern side of this bloc

关键词： South China Block Qinzhou-Fangcheng Junction Zone Magnetotelluric 1936 Lingshan M6^(3/4)earthquake Deep seismogenic environment

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Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1

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Animal Models and Experimental Medicine 2024年第2期7卷 179-188页

作者： Weidong Qian Xuefei Wang Ting Wang Jie Huang Qian Zhang yongdong Li Si Chen School of Food and Biological Engineering Shaanxi University of Science and TechnologyXi'anP.R.China Department of Dermatology Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenP.R.China Ningbo Municipal Center for Disease Control and Prevention NingboP.R.China Medical College of Shenzhen University ShenzhenP.R.China

Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of individuals with early infection of HBoV1 remains somewhat ***,we present a novel faster,lower cost,reliable method for the detection of HBoV1,which integrates a recombinase polymerase amplification(RPA)assay with the CRISPR/Cas12a system,designated the RPA-Cas12a-fluorescence *** RPA-Cas12a-fluorescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37℃without the need for sophisticated *** method also demonstrates excellent specificity without cross-reactivity to non-target ***,the method was appraised using 28 clinical samples,and displayed high accuracy with positive and negative predictive agreement of 90.9%and 100%,***,our proposed rapid and sensitive HBoV1 detection method,the RPA-Cas12a-fluorescence assay,shows promising potential for early on-site diagnosis of HBoV1 infection in the fields of public health and health *** established RPA-Cas12a-fluorescence assay is rapid and reliable method for human bocavirus 1 *** RPA-Cas12a-fluorescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

关键词： CRISPR-Cas12a detection human bocavirus 1 on-site diagnosis recombinase polymerase amplification

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The auxin transporter OsAUX1 regulates tillering in rice(Oryza sativa)

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Journal of Integrative Agriculture 2024年第5期23卷 1454-1467页

作者： Luqi Jia yongdong Dai Ziwei Peng Zhibo Cui Xuefei Zhang yangyang Li Weijiang Tian Guanghua He Yun Li Xianchun Sang Chongqing Key Laboratory of Crop Molecular Improvement/Rice Research Institute Academy of Agricultural SciencesSouthwest UniversityChongqing400715China Rice and Sorghum Research Institute Sichuan Academy of Agricultural Sciences/Key Laboratory of Southwest Rice Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsDeyang 618000China

Tillering is an important agronomic trait of rice(Oryza sativa)that affects the number of effective panicles,thereby affecting *** phytohormone auxin plays a key role in *** we identified the high tillering and semi-dwarf 1(htsd1)mutant with auxin-deficiency root characteristics,such as shortened lateral roots,reduced lateral root density,and enlarged root ***1 showed reduced sensitivity to auxin,but the external application of indole-3-acetic acid(IAA)inhibited its *** identified the mutated gene in htsd1 as AUXIN1(OsAUX1,LOC_Os01g63770),which encodes an auxin influx *** promoter sequence of OsAUX1 contains many SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)binding sites,and we demonstrated that SPL7 binds to the OsAUX1 *** BRANCHED1(OsTB1),a key gene that negatively regulates tillering,was significantly downregulated in *** was enhanced in the OsTB1 knockout mutant,and the external application of IAA inhibited tiller elongation in this *** OsTB1 restored the multi-tiller phenotype of *** results suggest that SPL7 directly binds to the OsAUX1 promoter and regulates tillering in rice by altering OsTB1 expression to modulate auxin signaling.

关键词： rice(Oryza sativa L.) tillering indole-3-acetic acid(IAA) OsAUX1 OsTB1

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White matter regeneration induced by aligned fibrin nanofiber hydrogel contributes to motor functional recovery in canine T12 spinal cord injury

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Regenerative Biomaterials 2022年第1期9卷 260-270页

作者： Zheng Cao Weitao Man Yuhui Xiong Yi Guo Shuhui yang Dongkang Liu He Zhao yongdong yang Shenglian Yao Chuzhong Li Lingyun Zhao Xiaodan Sun Hua Guo Guihuai Wang Xiumei Wang State Key Laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced MaterialsSchool of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Department of Neurosurgery Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China Center for Biomedical Imaging Research Tsinghua UniversityBeijing 100084China Department of Orthopedics Dongzhimen HospitalBeijing 100007China School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China Beijing Neurosurgical Institute Beijing Tiantan HospitalBeijing 100070China

A hierarchically aligned fibrin hydrogel(AFG)that possesses soft stiffness and aligned nanofiber structure has been successfully proven to facilitate neuroregeneration in vitro and in ***,its potential in promoting nerve regeneration in large animal models that is critical for clinical translation has not been sufficiently ***,the effects of AFG on directing neuroregeneration in canine hemisected T12 spinal cord injuries were *** obvious white matter regeneration consisting of a large area of consecutive,compact and aligned nerve fibers is induced by AFG,leading to a significant motor functional *** canines with AFG implantation start to stand well with their defective legs from 3 to 4 weeks postoperatively and even effortlessly climb the steps from 7 to 8 ***,high-resolution multi-shot diffusion tensor imaging illustrates the spatiotemporal dynamics of nerve regeneration rapidly crossing the lesion within 4 weeks in the AFG *** findings indicate that AFG could be a potential therapeutic vehicle for spinal cord injury by inducing rapid white matter regeneration and restoring locomotion,pointing out its promising prospect in clinic practice.

关键词： aligned fibrin hydrogel spinal cord injury white matter regeneration diffusion tensor imaging canine

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分析师预测的“技术”含量——基于Week52high的经验证据

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系统工程理论与实践 2024年第6期44卷 1853-1868页

作者：王谨乐杨振宇史永东东北财经大学金融科技学院大连116025 东北财经大学金融学院大连116025

本文以2007–2020年中国A股上市企业为样本,重点探讨技术分析是否影响分析师预测.研究发现:1)技术分析显著影响分析师预测.当股价接近52周历史高点(Week52high上升),分析师评级出现明显变化,预测偏差和预测分歧也随之下降;2)来自机构投... 详细信息

本文以2007–2020年中国A股上市企业为样本,重点探讨技术分析是否影响分析师预测.研究发现:1)技术分析显著影响分析师预测.当股价接近52周历史高点(Week52high上升),分析师评级出现明显变化,预测偏差和预测分歧也随之下降;2)来自机构投资者的压力越小,信息披露质量越高时,技术分析对分析师预测的影响越强.进一步研究发现:受“决策疲劳”影响,发布越多研报的分析师,其决策过程越依赖技术分析;与常识相悖,明星分析师从技术分析中“获益”更多;技术分析可能具有“信号”功能,触发技术指标能够有效增加分析师调研和研报发布,最终提升股价信息含量.

关键词：技术分析分析师预测 Week52high

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经寰枕后间隙蛛网膜下腔注射是脊髓损伤模型大鼠可供选择的给药方式

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中国组织工程研究 2022年第33期26卷 5376-5383页

作者：李传鸿俞兴杨永栋杨凯坦赵赫北京中医药大学东直门医院骨科北京市100700

背景:蛛网膜下腔给药是脊髓损伤动物实验中常用的干预方式,但目前缺乏可与缓释/控释剂型配合从而减少给药次数、避免蛛网膜下腔内置管的一次性较大剂量蛛网膜下腔内给药的方法。目的:探索一种对于大鼠脊髓损伤模型可以一次性较大剂量给... 详细信息

背景:蛛网膜下腔给药是脊髓损伤动物实验中常用的干预方式,但目前缺乏可与缓释/控释剂型配合从而减少给药次数、避免蛛网膜下腔内置管的一次性较大剂量蛛网膜下腔内给药的方法。目的:探索一种对于大鼠脊髓损伤模型可以一次性较大剂量给药的蛛网膜下腔注射方法。方法:将SD大鼠随机分为6组:脊髓损伤组采用NYU(New York University)脊髓打击器构建大鼠脊髓损伤模型,蛛网膜下腔注射组经寰枕后间隙将200μL模拟药液(生理盐水)注入蛛网膜下腔,脊髓损伤+蛛网膜下腔注射组在脊髓损伤造模后进行蛛网膜下腔注射,正常对照组无处理,蛛网膜下腔注射假手术组暴露寰枕后膜后关闭切口,脊髓损伤+蛛网膜下腔注射假手术组在脊髓损伤造模后重复蛛网膜下腔注射假手术组的操作。借助甲苯胺蓝染色探索药物注射1.5 h后在蛛网膜下腔内的弥散与分布情况,通过旷场试验、斜板试验、热板试验、体质量测量等验证是否发生蛛网膜下腔注射相关并发症。结果与结论:①各组经寰枕后间隙蛛网膜下腔注射未造成大鼠BBB评分、热板试验与斜板试验结果的异常。②蛛网膜下腔注射假手术组、蛛网膜下腔注射组大鼠术后1周时可恢复术前体质量(P>0.05),脊髓损伤组、脊髓损伤+蛛网膜下腔注射假手术组、脊髓损伤+蛛网膜下腔注射组术后2周时体质量可恢复到术前水平(P>0.05)。③术中及术后未观察到蛛网膜下腔注射引起大鼠中枢神经系统损伤的特殊征象,未见脑脊液/模拟药液外漏,术后大鼠可正常抬头,未出现精神萎靡和自残行为,蛛网膜下腔注射假手术组、蛛网膜下腔注射组未发生排尿排便障碍。④蛛网膜下腔注射后1.5 h,甲苯胺蓝溶液在大鼠蛛网膜下腔内广泛弥散,全段脊髓均有分布。⑤上述数据表明,经寰枕后间隙蛛网膜下腔注射的方法安全可靠,对脊髓损伤大鼠单次给药剂量200μL时,药物能快速到达脊髓损伤部位,引起严重并发症的风险较小,因此是大鼠脊髓损伤模型可供选择的蛛网膜下腔给药方式。

关键词：蛛网膜下腔注射寰枕后间隙寰枕后膜脊髓损伤血脊髓屏障动物实验安全性可靠性

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脊髓损伤中的小胶质细胞:M1/M2表型极化发挥神经毒性/神经保护作用

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中国组织工程研究 2022年第14期26卷 2265-2272页

作者：李传鸿俞兴杨永栋赵赫北京中医药大学东直门医院骨科北京市100700

背景:大量研究发现,小胶质细胞在脊髓损伤后被激活,发挥着神经保护和神经毒性的双向作用,与继发性损伤密切相关。目的:对脊髓损伤后M1/M2表型小胶质细胞的特征、以M1/M2表型为靶点的脊髓损伤干预研究进展及针对脊髓损伤后小胶质细胞未... 详细信息

背景:大量研究发现,小胶质细胞在脊髓损伤后被激活,发挥着神经保护和神经毒性的双向作用,与继发性损伤密切相关。目的:对脊髓损伤后M1/M2表型小胶质细胞的特征、以M1/M2表型为靶点的脊髓损伤干预研究进展及针对脊髓损伤后小胶质细胞未来可能的研究方向等内容进行综述。方法:第一作者在2021年5月以"microglia,spinal cord injury,M1 phenotype,M2 phenotype,polarization,activation,activated,regulation"为检索词在PubMed数据库中检索相关文献,在阅读文题及摘要后根据入选与排除标准筛选检索到的文章,最后共纳入文献76篇进行归纳总结与综述。结果与结论:(1)小胶质细胞独特的细胞起源与发育环境赋予它特殊的生物学功能,生理状态下的小胶质细胞依靠高度活跃的分枝状突起持续监测脊髓组织内微环境变化,发挥免疫监视功能。(2)脊髓损伤发生后,小胶质细胞快速响应微环境异常信号,极化为M1和M2表型,分别发挥神经毒性与神经保护作用。(3)大量基础实验证实,中药提取物、现代临床药物、基因表达调控、细胞移植、生物材料等干预措施可调控小胶质细胞的M1/M2表型,改善啮齿动物脊髓损伤模型的预后。但目前对于M1/M2型小胶质细胞的数量及比例的最佳调控目标尚无统一结论,同时缺乏靶向作用于小胶质细胞的活体给药方式,未来应深入探究促进M1/M2型小胶质细胞功能平衡的方法,将脊髓损伤后的炎症反应限定在有利于损伤脊髓修复的水平上,随着对脊髓损伤后小胶质细胞极化表型精准调控机制的深入研究,相应的基础研究成果有望用于临床治疗。

关键词：脊髓损伤继发性损伤小胶质细胞 M1表型 M2表型炎症反应神经保护综述

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节能减排视角下电力行业变电运维创新发展研究

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现代工业经济和信息化 2023年第10期13卷 166-168页

作者：杨永东十堰供电公司湖北十堰442000

研究旨在从节能减排的视角探讨电力行业变电运维的创新发展。首先,分析了电力行业与变电运维的现状及挑战,揭示了电力行业需要通过创新提升变电运维效率,实现节能减排。其次,通过理论研究和案例分析,深入探讨了创新发展的重要性以及节... 详细信息

研究旨在从节能减排的视角探讨电力行业变电运维的创新发展。首先,分析了电力行业与变电运维的现状及挑战,揭示了电力行业需要通过创新提升变电运维效率,实现节能减排。其次,通过理论研究和案例分析,深入探讨了创新发展的重要性以及节能减排与变电运维创新的关系。研究结果显示,引入智能预测维护、远程监控和诊断、高效变电设备等创新技术,以及优化运维管理、加强人员培训、塑造节能减排的企业文化等策略,能显著提升电力行业的运维效率,实现节能减排。研究提供了可行的研究方向和策略建议,为电力行业的节能减排作出一定的贡献。

关键词：电力行业变电运维创新发展节能减排

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Isobar EVO非融合动态固定治疗腰椎融合术后邻椎病的中期疗效

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中国组织工程研究 2022年第6期26卷 908-913页

作者：宋佳伟杨永栋俞兴杨济洲王逢贤曲弋毕连涌北京中医药大学东直门医院骨科北京市100700

背景:腰椎融合术后邻椎病的治疗是脊柱外科的研究热点,传统延长融合节段的手术有可能加速邻椎病的再次出现。以椎弓根螺钉为基础的非融合技术在治疗腰椎退行性疾病和预防邻近节段退变中表现出良好的中短期疗效,但采用非融合技术治疗腰... 详细信息

背景:腰椎融合术后邻椎病的治疗是脊柱外科的研究热点,传统延长融合节段的手术有可能加速邻椎病的再次出现。以椎弓根螺钉为基础的非融合技术在治疗腰椎退行性疾病和预防邻近节段退变中表现出良好的中短期疗效,但采用非融合技术治疗腰椎融合术后邻椎病的临床疗效亟待研究。目的:评估Isobar EVO非融合动态固定治疗腰椎融合术后邻椎病的效果。方法:选择2013年7月至2017年12月于东直门医院因保守治疗无效后再次手术的邻椎病患者,纳入了其中15例接受Isobar EVO原位非融合动态稳定治疗的患者,并回顾了病历、手术报告和X射线成像研究。比较手术前后的临床结果(包括腰痛和下肢痛目测类比评分、腰椎功能障碍指数、SF-36量表生理健康评分)和影像学测量指标(包括手术节段及上位邻近节段活动度、手术节段前凸角和腰椎前凸角、手术节段及上位邻近节段平均间隙高度指数)。结果与结论:15例患者完成了至少3年的随访。(1)临床结果:末次随访时各项评分较术前有显著改善(P0.05)。(2)影像学测量指标:末次随访时,手术节段活动度较术前明显减少[(3.16±0.45)°vs.(6.32±1.81)°,P0.05);以上指标在术后随访各时段之间相比差异均无统计学意义(P>0.05)。所有病例在随访周期内未见内置物松动,未再次发生邻椎病。(3)结论:Isobar EVO非融合动态固定是治疗邻椎病的有效方式,临床效果明显。术后3年以上随访结果显示该术式可在一定程度上预防再手术后邻椎病的发生,长期效果有待于进一步随访的结果和更高级别循证医学证据的支持。

关键词：邻椎病翻修手术非融合手术动态稳定 Isobar EVO 腰椎

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