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Characterization of tree shrew telomeres and telomerase

Journal of Genetics and Genomics 2021年第7期48卷 631-639页

作者： Jian Sun Wenjing Liu Yongbo Guo Hailin Zhang Dewei Jiang Ying Luo Rong Liu Ceshi Chen Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences&Yunnan Province National Resource Center for Non-Human PrimatesKunming Primate Research CenterNational Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyKunmingYunnan 650201China Kunming College of Life Sciences University of Chinese Academy SciencesKunmingYunnan 650204China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Laboratory of Molecular Genetics of Aging&Tumor Medical FacultyKunming University of Science and TechnologyKunmingYunnan 650500China

The use of tree shrews as experimental animals for biomedical research is a new practice.Several recent studies suggest that tree shrews are suitable for studying cancers,including breast cancer,glioblastoma,lung cancer,and hepatocellular carcinoma.However,the telomeres and the telomerase of tree shrews have not been studied to date.Here,we characterize telomeres and telomerase in tree shrews.The telomere length of tree shrews is approximately 23 kb,which is longer than that of primates and shorter than that of mice,and it is extended in breast tumor tissues according to Southern blot and flow-fluorescence in situ hybridization(FISH)analyses.Tree shrew spleen,bone marrow,testis,ovary,and uterus show high telomerase activities,which are increased in breast tumor tissues by telomeric repeat amplification protocol assays.The telomere length becomes shorter,and telomerase activity decreases with age.The tree shrew TERT and TERC are more highly similar to primates than to rodents.These findings lay a solid foundation for using tree shrews to study aging and cancers.

关键词： Tree shrews Telomere TERT TERC Breast cancer

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Overoxidized poly(3,4-ethylenedioxythiophene)-overoxidized polypyrrole composite films with enhanced electrocatalytic ability for rutin and luteolin determination

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Frontiers of Chemical Science and Engineering 2023年第6期17卷 735-748页

作者： Rongqian Meng Jianke Tang Hong Yang Lijun guo yongbo Song Qiaoling Li Yulan Niu Department of Chemistry and Chemical Engineering Taiyuan Institute of TechnologyTaiyuan 030008China School of Chemical Engineering and Technology North University of ChinaTaiyuan 030051China School of Science North University of ChinaTaiyuan 030051China

In this study, a simple and effective method was proposed to improve the electrocatalytic ability of overoxidized poly(3,4-ethylenedioxythiophene)-overoxidized polypyrrole composite films modified on glassy carbon electrode for rutin and luteolin determination. The composite electrode was prepared by cyclic voltammetry copolymerization with LiClO_(4)-water as the supporting electrolyte. The peak current of rutin and luteolin on the composite electrode gradually decreased or even disappeared with the increase in the positive potential limit. After incubation in NaOH–ethanol solution with a volume ratio of 1:1, the composite electrodes prepared at positive potential limit greater than 1.5 V exhibited enhanced differential pulse voltammetry peak currents, reduced charge transfer resistance, larger effective specific surface area and higher electron transfer rate constant. The composite electrode prepared in the potential range of 0–1.7 V showed optimal electrocatalytic performance. The X-ray photoelectron spectroscopy results indicated that the content of –SO_(2)/–SO and –C=N– groups in the composite film increased significantly after incubation. Further, the Raman spectra and Fourier transform infrared spectra revealed that the thiophene ring structure changed from benzene-type to quinone-type, and the quinone-type pyrrole ring was formed. The electrocatalytic mechanism of the composite film was proposed based on the experimental results and further verified by Density Functional Theory calculation.

关键词： overoxidized poly(3,4-ethylenedioxythiophene)-overoxidized polypyrrole rutin luteolin incubation electrocatalytic mechanism

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A New Solution Based on Optimal Link-State Routing for Named Data MANET

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China Communications 2021年第4期18卷 213-229页

作者： guo Xian Yang Shengya Cao Laicheng Wang Jing Jiang yongbo School of Computer and Communication Lanzhou University of TechnologyLanzhou730050China

NDN is an important instance of Information-Centric Networking.When integrating NDN into MANET,exploring new routing is a necessary task for this research area.The LSAs flooding is a common method to obtain network topology during route establishment.However,the LSAs flooding often results in a broadcast storm in high-density MANET.Using the MPR set proposed in the OLSR can effectively reduce the number of LSAs in the process of route establishment and can further solve the broadcast storm.In this paper,an enhanced neighbor discovery protocol firstly is designed to establish a MPR set.The new protocol can effectively avoid the problem incurred by unidirectional links that impact the network performance in a wireless environment.And then a new and proactive routing NOLSR based on OLSR for NDN-MANET is proposed to support NDN in MANET.And another important work is that NOLSR is implemented on top of NDN Forwarding Daemon NFD.Finally,we make a comparative analysis between NOLSR and the two most relative schemes such as traditional LSA-flooding and the scheme[1]by emulation experiments in the NDN emulator mini-NDN.

关键词： named-data networking NLSR MANET OLSR

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A Receiver-Forwarding Decision Scheme Based on Bayesian for NDN-VANET

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China Communications 2020年第8期17卷 106-120页

作者： Xian guo Yuxi Chen Laicheng Cao Di Zhang yongbo Jiang School of Computer and Communication Lanzhou University of TechnologyLanzhou 730050China

Named Data Network(NDN) has caused wide concerns in VANET community because NDN uses a content-centric mechanism that naming content rather than the host. However, integrating NDN into VANET(NDN-VANET) also faces several challenges including consumer/provider mobile, broadcast storm problem and so on. In this paper, we propose a Bayesian-based Receiver Forwarding Decision(BRFD) scheme to mitigate the broadcast storm problem incurred by interest packets in NDN-VANET. In the BRFD, vehicles received an interest packet are required to make forwarding decisions based on Bayesian decision theory according to current network conditions obtained by neighbor interaction. However, the receiver-forwarding decision in BRFD can also cause a conflict issue because multiple vehicles forward copies of the same packet at the same time. So a back-off mechanism is introduced in BRFD. Experimental results show that the BRFD algorithm has better performance in several aspects in contrast to probability-based forwarding scheme and "bread crumb" routing.

关键词： named-data networking content routing Bayesian decision theory broadcast storm VANET

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Regulating the properties of XQ-2d for targeted delivery of therapeutic agents to pancreatic cancers

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National Science Review 2023年第8期10卷 166-172页

作者： Qiuxia Yang yongbo Peng Zhengyu Deng Dailiang Zhang Cheng-Yu Long guo-Rong Zhang Juan Li Xue-Qiang Wang Weihong Tan Zhejiang Cancer Hospital Hangzhou Institute of Medicine(HIM) Chinese Academy of Sciences Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan ProvinceHunan University Institute of Molecular Medicine(IMM) Renji HospitalSchool of MedicineCollege of Chemistry and Chemical Engineering Shanghai Jiao Tong University

Enhanced recognition ability, cell uptake capacity, and biostability are characteristics attributed to aptamer-based targeted anticancer agents, and are possibly associated with increased accumulation at the tumor site, improved therapeutic efficacy and reduced negative side effects. Herein, a phosphorothioate backbone modification strategy was applied to regulate the biomedical properties of pancreatic cancer cell–targeting aptamer for efficient in vivo drug delivery. Specifically, the CD71-targeting aptamer XQ-2d was modified into a fully thio-substituted aptamer S-XQ-2d, improving the plasma stability of S-XQ-2d and mitomycin C(MMC)-functionalized S-XQ-2d(MFSX), thus considerably prolonging their half-life in mice. Moreover, the binding and uptake capacities of S-XQ-2d were significantly enhanced. MFSX showed the same level of cytotoxicity as that of MMC against targeted cancer cells, but lower toxicity to non-targeted cells, highlighting its specificity and biosafety. Brief mechanistic studies demonstrated that XQ-2d and S-XQ-2d had different interaction modes and internalization pathways with the targeted cells.

关键词： phosphorothiolation MMC-functionalized aptamer targeted pancreatic cancer therapy

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Design and Implementation of NOLSR for NDNMANET Routing

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China Communications 2020年第6期17卷 246-260页

作者： Xian guo Shengya Yang Laicheng Cao Jing Wang yongbo Jiang School of Computer and Communication Lanzhou University of TechnologyLanzhou730050 China

Named Data Network(NDN)is an important instance of future internet architectures.NDN-MANET routing is a hot research area.Link State Advertisements(LSAs)flooding is a common method to obtain network topology during routes establishment.However,LSAs flooding often causes a broadcast storm in high-density MANET.Using a MultiPoint Relay(MPR)set proposed in the Optimized Link State Routing protocol(OLSR)can effectively reduce the number of LSAs in the process of route establishment.So,this solution can solve the broadcast storm.Based on OLSR,we design and implement a novel routing Named-data Optimized Link State Routing protocol(NOLSR)for NDN-MANET.In NOLSR,a new neighbor discovery protocol to establish the MPR set is proposed.Only nodes in the MPR set will forward LSAs so that other nodes can obtain a network topology and further establish the routing table.The neighbor discovery protocol can also avoid the problem of unidirectional links which impacts the performance of a network.Finally,we make a comparative analysis between classic flooding and NOLSR by simulation experiment in an NDN emulator mini-NDN.

关键词： named-data networking NLSR MANET OLSR

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High Arterial Oxygen Saturation in the Acclimatized Lowlanders Living at High Altitude

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Phenomics 2023年第4期3卷 329-332页

作者： Yaoxi He Chaoying Cui Yongbo Guo Wangshan Zheng Tian Yue Hui Zhang Ouzhuluobu Tianyi Wu Xuebin Qi Bing Su State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China High Altitude Medical Research Center School of MedicineTibetan UniversityLhasa 850000China National Key Laboratory of High Altitude Medicine High Altitude Medical Research InstituteXining 810012China

Blood oxygen saturation(SpO_(2))is a key indicator of oxygen availability in the body.It is known that a low SpO_(2)at high altitude is associated with morbidity and mortality risks due to physiological hypoxemia.Previously,it was proposed that the lowlander immigrants living at high altitude should have a lower SpO_(2)level compared to the highlander natives,but this proposal has not been rigorously tested due to the lack of data from the lowlander immigrants living at high altitude.In this study,we compared arterial oxygen saturation of 5929 Tibetan natives and 1034 Han Chinese immigrants living at altitudes ranging from 1120 m to 5020 m.Unexpectedly,the Han immigrants had a higher SpO_(2)than the Tibetan natives at the same high altitudes.At the same time,there is a higher prevalence of chronic mountain sickness in Han than in Tibetans at the same altitude.This result suggests that the relatively higher SpO_(2)level of the acclimatized Han is associated with a physiological cost,and the SpO_(2)level of Tibetans tends to be sub-optimal.Consequently,SpO_(2)alone is not a robust indicator of physiological performance at high altitude.

关键词： Oxygen saturation High altitude Acclimatization Adaptation

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新课标视域下“学、练、赛”一体化教学的主要特征、现实问题及实现路径

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上海体育大学学报 2024年第2期48卷 34-44页

作者：翟宗鹏郭永波深圳大学体育学院广东深圳518060 华南师范大学体育科学学院广东广州510006

“学、练、赛”一体化教学的内涵体现在系统化的教学过程、真实化的教学设计、专项化的教学方式,其特征表现为聚焦“学、练、赛”三者的有机结合和整体运动能力的形成,注重通过有针对性的教学安排进行具有深度的体育教学,强调以“赛”... 详细信息

“学、练、赛”一体化教学的内涵体现在系统化的教学过程、真实化的教学设计、专项化的教学方式,其特征表现为聚焦“学、练、赛”三者的有机结合和整体运动能力的形成,注重通过有针对性的教学安排进行具有深度的体育教学,强调以“赛”为核心唤醒和增强学生的体育运动意识。“学、练、赛”一体化教学实践有助于体育教学目标的分解与转换、内容的重构与升级、方法和过程的优化以及教学评价的改进。针对当前“学、练、赛”一体化教学存在的问题提出实现路径:以核心素养为导向定位体育教学目标的应然价值;对“学、练、赛”各环节进行针对性设计;动态调整“学、练、赛”整体结构;选择适宜学生的“学、练、赛”教学流程;将表现性评价作为实现“学、练、赛”一体化的有力抓手。

关键词：体育教学设计 “学、练、赛”一体化体育教学改革体育教学论新课标

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A 75 GHz regenerative dynamic frequency divider with active transformer using InGaAs/InP HBT technology

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Journal of Semiconductors 2017年第8期38卷 55-60页

作者： Xi Wang Bichan Zhang Hua Zhao yongbo Su Asif Muhammad Dong guo Zhi Jin Institute of Microelectronics Chinese Academy of Sciences

This letter presents a high speed 2 ： 1 regenerative dynamic frequency divider with an active transformer fabricated in 0.7μm InP DHBT technology with fTof 165 GHz and fmax of 230 GHz. The circuit includes a two-stage active transtbrmer, input buffer, divider core and output buffer. The core part of the frequency divider is composed of a double-balanced active mixer （widely known as the Gilbert cell） and a regenerative feedback loop. The active transformer with two stages can contribute to positive gain and greatly improve phase difference. Instead of the passive transformer, the active one occupies a much smaller chip area. The area of the chip is only 469 × 414μm2 and it entirely consumes a total DC power of only 94.6 mW from a single -4.8 V DC supply. The measured results present that the divider achieves an operating frequency bandwidth from 75 to 80 GHz, and performs a -23 dBm maximunl output power at 37.5 GHz with a 0 dBm input signal of 75 GHz.

关键词： InP hetero-junction bipolar transistors dynamic frequency divider

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Seasonality and Sex-Biased Fluctuation of Birth Weight in Tibetan Populations

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Phenomics 2022年第1期2卷 64-71页

作者： Yaoxi He Jun Li Tian Yue Wangshan Zheng Yongbo Guo Hui Zhang Li Chen Chunxia Li Hongyan Li Chaoying Cui Ouzhuluobu Xuebin Qi Bing Su State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Fukang Obstetrics Gynecology and Children Branch HospitalTibetan Fukang HospitalLhasa 850000China High Altitude Medical Research Center School of MedicineTibetan UniversityLhasa 850000China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China Kunming College of Life Science University of Chinese Academy of SciencesBeijing 100101China

Birth weight(BW)is a key determinant of infant mortality.Previous studies have reported seasonal fluctuation of BW.However,the responsible environmental factors remain disputable.High-altitude environment provides a great opportunity to test the current hypotheses due to its distinctive climate conditions.We collected BW data of~9000 Tibetan singletons born at Lhasa(elevation:3660 m)from 2014 to 2018.Using regression models,we analyzed BW seasonality of highland Tibetans.Multivariate models with meteorological factors as independent variables were employed to examine responsible environmental factors accounting for seasonal variation.We compared BW,low-BW prevalence and sex ratio between high-land and lowland populations,and we observed a significant seasonal pattern of BW in Tibetans,with a peak in winter and a trough in summer.Notably,there is a marked sex-biased pattern of BW seasonality(more striking in males than in females).Sunlight exposure in the 3rd trimester and barometric pressure exposure in the 2nd trimester are significantly correlated with BW,and the latter can be explained by seasonal change of oxygen partial pressure.In particular,due to the male-biased BW seasonality,we found a more serious BW reduction and higher prevalence of low-BW in males,and a skewed sex ratio in highlanders.The infant BW of highland Tibetans has a clear pattern of seasonality.The winter BW is larger than the summer BW,due to the longer sunlight exposure during the late-trimester.Male infants are more sensitive to hypoxia than female infants during the 2nd trimester,leading to more BW reduction and higher mortality.

关键词： Birth weight Seasonality Sex-bias High altitude Tibetans

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