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Identification of key gene networks controlling monoterpene biosynthesis during grape ripening by integrating transcriptome and metabolite profiling

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Horticultural Plant Journal 2023年第5期9卷 931-946页

作者： Xiangpeng Leng Jiameng Cong Lixia Cheng Haoliang Wan yuanxia Liu yongbing yuan Jinggui Fang Institute of Grape Science and Engineering College of HorticultureQingdao Agricultural UniversityQingdaoShandong 266109China Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province College of HorticultureQingdao Agricultural UniversityQingdaoShandong 266109China Laboratory of Quality&Safety Risk Assessment for Fruit(Qingdao) Ministry of Agriculture and Rural AffairsNational Technology Centre for Whole Process Quality Control of FSEN Horticultural Products(Qingdao)Qingdao Agricultural UniversityQingdaoShandong 266109China

Monoterpenes are typical aroma components of muscat grape cultivars,providing pleasant floral and fruity aromas to grapes and ***,the molecular mechanism of monoterpene biosynthesis between muscat and non-muscat grape remains ***,the muscat grape cultivar‘Jumeigui’and the non-muscat grape cultivar‘Kyoho’were chosen as plant materials for a comprehensive transcriptome and metabolite *** gas chromatography-mass spectrometry(GC-MS)analysis demonstrated that a total of 27 and 23 monoterpene compounds were identified and quantified in the‘Jumeigui’and‘Kyoho’grape,respectively.‘Jumeigui’grape accumulated significantly higher concentrations of monoterpenes than‘Kyoho’***,geraniol,linalool,geranic acid,and β-citronellol might be important odorants contributing to the floral character of the‘Jumeigui’grape due to the high levels odor activity values(OAVs).Transcriptome analysis demonstrated that the expression profiles of VvDXS,***1,Vv TPS-b/g showed a positive correlation with monoterpene accumulation in *** addition,the expression patterns of the genes involved in jasmonic acid(JA)synthesis and signal were also positively correlated with monoterpene *** these results will help guide the functional verification of candidate genes related to monoterpene biosynthesis,as well as identify the master transcriptional and hormonal regulators of this pathway in grapes.

关键词： Grape Muscat Monoterpene Transcriptome Monoterpene biosynthesis

“双一流”建设背景下化学化工类研究生联合培养模式探析与实践

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化工管理 2024年第15期 44-47页

作者：袁永兵张岑周从山许文苑杨长安湖南理工学院化学化工学院湖南岳阳414006

基于湖南理工学院现有科研资源与创新实践平台对接石化企业,聚焦研发项目,校企双方联合培养研究生。文章从培养目标、培养方法及评价体系等方面对化学化工类研究生创新实践能力开展综合性、系统性的理论研究。同时,从校内教学、校外实... 详细信息

基于湖南理工学院现有科研资源与创新实践平台对接石化企业,聚焦研发项目,校企双方联合培养研究生。文章从培养目标、培养方法及评价体系等方面对化学化工类研究生创新实践能力开展综合性、系统性的理论研究。同时,从校内教学、校外实践、成果转化、论文评阅、就业情况等多个方面对校企联合培养的研究生进行分阶段、全过程、全覆盖考察评价,通过建立健全全面、科学、可靠的评价体系,激励研究生积极参与校企合作项目,提高创新实践能力水平。

关键词：研究生联合培养 “双一流” 化学化工

An apple (Malus domestica) AP2/ERF transcription factor modulates carotenoid accumulation

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Horticulture Research 2021年第1期8卷 3055-3066页

作者： Qingyuan Dang Haiyun Sha Jiyun Nie Yongzhang Wang yongbing yuan Dongjie Jia Qingdao Key Lab of Modern Agriculture Quality and Safety Engineering College of HorticultureQingdao Agricultural UniversityQingdao 266109China

Color is an important trait for horticultural *** are one of the main pigments for coloration and have important implications for photosynthesis in plants and benefits for human ***,we identified an APETALA2(AP2)/ETHYLENE RESPONSE FACTOR(ERF)transcription factor named MdAP2-34 in apple(Malus domestica Borkh.).MdAP2-34 expression exhibited a close correlation with carotenoid content in‘Benin Shogun’and‘Yanfu 3’fruit ***2-34 promotes carotenoid accumulation in MdAP2-34-OVX transgenic apple calli and fruits by participating in the carotenoid biosynthesis *** major carotenoid contents of phytoene andβ-carotene were much higher in overexpressing MdAP2-34 transgenic calli and fruit skin,yet the predominant compound of lutein showed no obvious difference,indicating that MdAP2-34 regulates phytoene andβ-carotene accumulation but not ***2-1(phytoene synthase 2)is a major gene in the carotenoid biosynthesis pathway in apple fruit,and the MdPSY2-1 gene is directly bound and transcriptionally activated by *** addition,overexpressing MdPSY2-1 in apple calli mainly increases phytoene and total carotenoid *** findings will advance and extend our understanding of the complex molecular mechanisms of carotenoid biosynthesis in apple,and this research is valuable for accelerating the apple breeding process.

关键词： apple breeding expressing

Differential gene expression analysis of ‘Chili’ (Pyrus bretschneideri) fruit pericarp with two types of bagging treatments

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Horticulture Research 2017年第1期4卷 348-357页

作者： Yuling Wang Xinfu Zhang Ran Wang Yingxin Bai Chenglian Liu yongbing yuan Yingjie Yang Shaolan Yang Department of Horticulture Qingdao Agricultural UniversityQingdao266109China Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants Qingdao266109China

Preharvest bagging is a simple,grower-friendly and safe physical protection technique commonly applied to many fruits,and the application of different fruit bags can have various *** explore the molecular mechanisms underlying the fruit quality effects of different bagging treatments,digital gene expression(DGE)profiling of bagged and unbagged‘Chili’(Pyrus bretschneideri Rehd.)pear pericarp during development was *** to unbagged fruit,a total of 3022 and 769 differentially expressed genes(DEGs)were detected in the polyethylene(PE)-bagged and non-woven fabric-bagged fruit,*** annotated as photosynthesis-antenna proteins and photosynthesis metabolism pathway were upregulated in non-woven fabric-bagged fruit but downregulated in the PE-bagged ***-woven fabric bagging inhibited lignin synthesis in‘Chili’pear pericarp by downregulating DEGs involved in phenylpropanoid biosynthesis;consequently,the fruit lenticels in non-woven fabric-bagged fruit were smaller than those in the other *** results indicate that the non-woven fabric bagging method has a positive effect on the appearance of‘Chili’pear fruit but neither of the two bagging treatments is conducive to the accumulation of soluble sugar.

关键词： sugar metabolism bagging

The origin and evolution of Earth's nitrogen

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National Science Review 2024年第6期11卷 56-75页

作者： yuan Li State Key Laboratory of Isotope Geochemistry Guangzhou Institute of GeochemistryChinese Academy of Sciences Bayerisches Geoinstitut Universit?t Bayreuth

Nitrogen is a vital element for life on Earth. Its cycling between the surface(atmosphere + crust) and the mantle has a profound influence on the atmosphere and climate. However, our understanding of the origin and evolution of Earth's nitrogen is still incomplete. This review presents an overview of the current understanding of Earth's nitrogen budget and the isotope composition of different reservoirs, laboratory constraints on deep nitrogen geochemistry, and our understanding of the origin of Earth's nitrogen and the deep nitrogen cycle through plate subduction and volcanism. The Earth may have acquired its nitrogen heterogeneously during the main accretion phase, initially from reduced, enstatite-chondrite-like impactors,and subsequently from increasingly oxidized impactors and minimal CI-chondrite-like materials. Like Earth's surface, the mantle and core are also significant nitrogen reservoirs. The nitrogen abundance and isotope composition of these three reservoirs may have been fundamentally established during the main accretion phase and have been insignificantly modified afterwards by the deep nitrogen cycle, although there is a net nitrogen ingassing into Earth's mantle in modern subduction zones. However, it is estimated that the early atmosphere of Earth may have contained ～1.4 times the present-day atmospheric nitrogen(PAN),with ～0.4 PAN being sequestered into the crust via biotic nitrogen fixation. In order to gain a better understanding of the origin and evolution of Earth's nitrogen, directions for future research are suggested.

关键词： nitrogen isotopes silicate Earth atmosphere magma ocean

Robustness of the octupole collectivity in 144Ba within the cranking covariant density functional theory in 3D lattice

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Nuclear Science and Techniques 2024年第8期35卷 124-131页

作者： Ze‑Kai Li yuan‑yuan Wang Mathematics and Physics Department North China Electric Power UniversityBeijing 102206China Hebei Key Laboratory of Physics and Energy Technology North China Electric Power UniversityBaoding 071000China

The octupole deformation and collectivity in octupole double-magic nucleus 144Ba are investigated using the Cranking covariant density functional theory in a three-dimensional lattice *** reduced B(E3)transition probability is implemented for the first time in semiclassical approximation based on the microscopically calculated electric octupole *** available data,including the I-ωrelation and electric transitional probabilities B(E2)and B(E3)are well ***,it is shown that the ground state of 144Ba exhibits axial octupole and quadrupole deformations that persist up to high spins(I≈24h).

关键词： Octupole collectivity Cranking covariant density functional theory Rotational spectrum Electric transitional probabilities

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情绪调节对小学生心理弹性的影响

科教导刊 2017年第5Z期 158-160页

作者：袁勇兵广东省河源市源城区城南小学广西河源517000

目的:探讨情绪调节对3~5年级小学生心理弹性的影响。方法:本研究采用了情绪调节量表和心理弹性量表对715名3~5年级的小学生进行调查研究。结果:(1)在不同性别间,心理弹性不存在显著差异,而情绪调节及其下的认知重评存在显著差异,但情绪... 详细信息

目的:探讨情绪调节对3~5年级小学生心理弹性的影响。方法:本研究采用了情绪调节量表和心理弹性量表对715名3~5年级的小学生进行调查研究。结果:(1)在不同性别间,心理弹性不存在显著差异,而情绪调节及其下的认知重评存在显著差异,但情绪调节的另一个维度表达抑制却不存在显著性的差异。(2)心理弹性、情绪调节及其下的两个维度在独生与非独生子女之间,以及在不同年级之间均不存在显著性的差异。(3)认知重评和表达抑制对心理弹性有正向的预测作用,而且认知重评的预测作用比表达抑制的强。结论:情绪调节对3~5年级的小学生心理弹性有积极的影响。

关键词：小学生情绪调节心理弹性

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后进生转化的心理学策略

科教导刊 2013年第20期 215-215,243页

作者：袁勇兵广东省河源市源城区城南小学广东河源517000

我国后进生有心理问题的比例在逐年上升。近年来,后进生问题越来越受到教育学和心理学的关注。本文回归了后进生的主要种类及其可能的形成原因,对如何在中小学进行后进生转化提供了一些思路和建议。

关键词：后进生转化策略

Collective Molecular Machines: Multidimensionality and Reconfigurability

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Nano-Micro Letters 2024年第8期16卷 309-340页

作者： Bin Wang yuan Lu Department of Chemical Engineering Tsinghua UniversityBeijing 100084People’s Republic of China Key Laboratory of Industrial Biocatalysis Ministry of EducationTsinghua UniversityBeijing 100084People’s Republic of China

Molecular machines are key to cellular activity where they are involved in converting chemical and light energy into efficient mechanical *** the last 60 years,designing molecular structures capable of generating unidirectional mechanical motion at the nanoscale has been the topic of intense *** progress has been made,attributed to advances in various fields such as supramolecular chemistry,biology and nanotechnology,and ***,individual molecular machines are only capable of producing nanometer work and generally have only a single *** order to address these problems,collective behaviors realized by integrating several or more of these individual mechanical units in space and time have become a new *** this review,we comprehensively discuss recent developments in the collective behaviors of molecular *** particular,collective behavior is divided into two *** is the appropriate integration of molecular machines to efficiently amplify molecular motions and deformations to construct novel functional *** other is the construction of swarming modes at the supramolecular level to perform nanoscale or microscale *** discuss design strategies for both modes and focus on the modulation of features and ***,in order to address existing challenges,the idea of transferring experience gained in the field of micro/nano robotics is presented,offering prospects for future developments in the collective behavior of molecular machines.

关键词： Molecular machines Collective control Collective behaviors DNA Biomolecular motors