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冷藏技术 2024年第2期47卷 80-83页

作者：王强谭永安王家政冰山冷热科技股份有限公司大连116600 冰山松洋冷机系统(大连)有限公司大连116600

冷库在实际运行中噪声问题不容忽视,特别是离居民区较近时,应使噪声排放符合国家标准。本文以某冷库为例,介绍了厂界噪音计算方法、改造措施和改造前后噪音测试结果对比。通过降噪处理,使得冷库噪声排放满足需求,为冷库的建设和改造提... 详细信息

冷库在实际运行中噪声问题不容忽视,特别是离居民区较近时,应使噪声排放符合国家标准。本文以某冷库为例,介绍了厂界噪音计算方法、改造措施和改造前后噪音测试结果对比。通过降噪处理,使得冷库噪声排放满足需求,为冷库的建设和改造提供了一种适用方案。

关键词：冷库噪音降噪

Machine learning-assisted sparse observation assimilation for real-time aerodynamic field perception

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Science China(Technological Sciences) 2024年第5期67卷 1458-1469页

作者： ZHAO QingYu HUANG Jun GUO YuXin PAN YuXuan JI JingJing HUANG yongan State Key Laboratory of Intelligent Manufacturing Equipment and Technology School of Mechanical Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China

Accurate aerodynamic distribution perception and real-time flight state evaluation are crucial for flight safety,e.g.,stall ***,the observations are usually sparse due to limitations in sensor mounting space and cost,and a reconstruction technology is urgently ***,a machine learning-assisted assimilation method based on sparse observations has been *** from the traditional reconstruction methods focusing on boundary condition correction,the proposed method formulates the flow field pressure distribution as a linear superposition of flow field modes,thereby forming a real-time reconstruction pattern that combines offline modal extraction using computational fluid dynamics(CFD)with real-time determination of modal weights using a neural *** this study,CFD simulations were conducted under 800different operating conditions for common modal extraction and model *** weights of these modes were determined online based on merely five observations for reconstructing the full pressure field.A pressure reconstruction with a relative error of 6.1%and a mean square error of 0.003 was achieved within the prescribed condition *** computational cost was just2 ms for each reconstruction run,significantly faster than the 20 min required by the classical reconstruction ensemble transform Kalman *** also showed that the method maintains almost the same accuracy amidst 1.5%measurement *** practical examples,shock waves and the change of lift coefficient were analyzed using the proposed method,providing remarkable evidence for the capability of the method in supporting stall *** validate the method’s effectiveness and explore its potential in real-time and accurate monitoring of an aircraft.

关键词： aerodynamic force sparse observation neural networks pressure field reconstruction

Identification of heat shock cognate protein 70 gene （Alhsc70） of Apolygus lucorum and its expression in response to different temperature and pesticide stresses

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Insect Science 2016年第1期23卷 37-49页

作者： Yang Sun Jing Zhao Yang Sheng Ying-Fang Xiao Yong-Jun Zhang Li-Xin Bai yongan tan Liu-Bin Xiao Guang-Chun Xu Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing China Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing China Entomology and Nematology Mid-Florida Research and Education Center University of Florida Apopka Florida USA

Heat shock cognate protein 70 （Hsc70） is a very important stress-resistance protein of insects against environmental stresses. We employed fluorescent real-time quan- titative polymerase chain reaction and Western-blot techniques to analyze the transcrip- tional and translational expression profiles of AIHSC70 under extreme temperature （4℃ and 40℃） or 4 pesticide stresses in Apolygus lucorum. The results showed that the expres- sion of AIHSC70 were significantly induced by cyhalothrin or extremely high temperature （40℃） in both transcriptional and translational levels （P 〈 0.05）, while the transcriptional and translational level of AIHSC70 decreased significantly in treatments of chlorpyrifos or extreme cold temperature （4℃）（P 〈 0.05）. Moreover, afterApolygus lucorum treated by imidacloprid or emamectin benzoate, the expression of AIHSC70 was only up-regulated significantly at the transcriptional level （P 〈 0.05）, although obviously up-regulated at the translational level of AIHSC70. Therefore, this study confirmed that the Alhsc70 gene played important roles in response to both temperature and pesticide stresses, especially for cyhalothrin or extremely high temperature （40℃）. In addition, the significant polynomial regression correlations between temperature and the Alhsc70 expression level were shown in all the nymph and adult stages （P 〈 0.01）, indicating temperature was an important factor to affect the relative expression of Alhsc70.

关键词： Apolygus lucorum expression files extreme temperature hsc70 pesticide

A mix & act liposomes of phospholipase A2-phosphatidylserine for acute brain detoxification by blood‒brain barrier selective-opening

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1827-1844页

作者： Zinan Zhang Wenbin Cao Huanchun Xing Shuai Guo Lijuan Huang Lin Wang Xin Sui Kui Lu Yuan Luo yongan Wang Jun Yang State Key Laboratory of Toxicology and Medical Countermeasures Beijing Institutes of Pharmacology and ToxicologyBeijing 100850China Tianjin University of Science and Technology Tianjin 300222China Hebei University of Science and Technology Shijiazhuang 050018China

In the treatment of central nervous system disease,the blood-brain barrier(BBB)is a major obstruction to drug delivery that must be *** this study,we propose a brain-targeted delivery strat-egy based on selective opening of the *** strategy allows some simple bare nanoparticles to enter the brain when mixed with special opening material;however,the BBB still maintains the ability to completely block molecules from passing *** on the screening of BBB opening and matrix delivery mate-rials,we determined that phospholipase A2-catalyzed 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine li-posomes can efficiently carry drugs into the brain *** an effective dose,this delivery system is safe,especially with its effect on the BBB being *** mix&act delivery system has a simple structure and rapid preparation,making it a strong potential candidate for drug delivery across the BBB.

关键词： Liposomes Phospholipase A2 Phosphoserine Blood-brainbarrier Selectively open Mix&act

A universal performance-enhancing method for Li-S batteries:the cathode material of Li_(2)S@Li_(2)S_(2)@Li_(2)S_(6) double-shell structure

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Science China Chemistry 2024年第4期67卷 1229-1241页

作者： Shunjin Yang Yujiang Sun Qiaran Zhang Xiaohu Hu Xing Chen Guoran Li Xiao Sun Yuzhe Zhang Shijie Xu Xinyu Wang yongan Yang Institute of Molecular Plus School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China College of Materials Science and Engineering Nankai UniversityTianjin 300350China Haihe Laboratory of Sustainable Chemical Transformations Tianjin 300192China Tianjin Key Laboratory of Advanced Carbon and Electrochemical Energy Storage Tianjin 300072China

Lithium sulfide(Li_(2)S)as a cathode material for lithium-sulfur(Li-S)batteries,one of the most promising advanced batteries in the future,has received tremendous attention in the past ***,developing the practical Li_(2)S cathode confronts challenges of low conductivity for Li-ions and electrons,high sensitivity to environmental moisture,big overpotential barrier to electrochemical activation,and poor cyclability due to the shuttle effect of intermediate *** article herein reports a simple and effective strategy for making Li_(2)S@Li_(2)S_(2)@Li_(2)S_(6) double-shelled microparticles,which can significantly mitigate these *** are synthesized by dissolving Li2S together with S in dimethoxyethane,then drying off the solvent,and finally calcining the collected *** with pure Li_(2)S,such a double-shell material presents a 26.7% improvement in cycling capacity,0.5 V lower in activation overpotential,and prolonged tolerance in the ambient *** density functional theory calculation shows that the performance enhancement lies in the higher stability of Li2S6in contact with moisture and some autocatalytic effect of Li_(2)S_(2)@Li_(2)S_(6).Such a double-shell structure becomes a universal performanceenhancing approach when being combined with other means,such as cathodes composited with catalytic MoS_(2),separators modified with selenium-doped sulfurized-polyacrylonitrile/montmorillonite,electrolytes containing fluorenone additive,and Li anodes coated with a layer of Li_(3)*** corresponding capacity retention shows up to 80%improvement compared with pure Li_(2)S.

关键词： lithium-sulfur batteries lithium sulfide lithium polysulfides cathode performance double shell structure

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我国历次刑法修正案总述评

中国法律评论 2024年第2期56卷 178-197页

作者：许永安全国人大常委会法工委刑法室

1997年刑法以来,我国逐渐形成了以刑法修正案为主的刑法修改模式,这也为其他法典化法律的修改方式提供了参考。修正案的起草坚持党的领导、人民当家作主和依法治国有机统一,科学立法、民主立法、依法立法的有机统一,坚持宽严相济刑事政... 详细信息

1997年刑法以来,我国逐渐形成了以刑法修正案为主的刑法修改模式,这也为其他法典化法律的修改方式提供了参考。修正案的起草坚持党的领导、人民当家作主和依法治国有机统一,科学立法、民主立法、依法立法的有机统一,坚持宽严相济刑事政策,坚持系统观念、问题导向。刑法修正案总体以完善分则规定为主,其中反腐败相关规定的修改完善是我国刑法修改的重要主线。同时根据实践需要,不断完善恐怖活动犯罪、黑社会性质组织犯罪、安全生产犯罪、金融犯罪、信息网络犯罪、知识产权犯罪等刑法规定;适应我国社会主要矛盾变化的需要,还注意强化对民生领域的刑法保护,对生态环境犯罪、食品药品犯罪,以及侵犯妇女、儿童权益犯罪等及时作出修改完善。在总则方面,在减少死刑罪名的过程中,对死刑相关的刑罚制度作出重大修改;调整刑罚结构,改革完善刑罚制度。

关键词：刑法修正案修法模式问题导向宽严相济刑事政策

A universal performance-enhancing method for Li-S batteries: the cathode material of LiS@LiS@LiSdouble-shell structure

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Science China Chemistry 2024年

作者： Shunjin Yang Yujiang Sun Qiaran Zhang Xiaohu Hu Xing Chen Guoran Li Xiao Sun Yuzhe Zhang Shijie Xu Xinyu Wang yongan Yang College of Materials Science and Engineering Nankai University Haihe Laboratory of Sustainable Chemical Transformations Tianjin Key Laboratory of Advanced Carbon and Electrochemical Energy Storage Institute of Molecular Plus School of Chemical Engineering and Technology Tianjin University

Lithium sulfide(Li2S) as a cathode material for lithium-sulfur(Li-S) batteries, one of the most promising advanced batteries in the future, has received tremendous attention in the past decades. However, developing the practical Li2S cathode confronts challenges of low conductivity for Li-ions and electrons, high sensitivity to environmental moisture, big overpotential barrier to electrochemical activation, and poor cyclability due to the shuttle effect of intermediate species. This article herein reports a simple and effective strategy for making Li2S@Li2S2@Li2S6double-shelled microparticles, which can significantly mitigate these problems. They are synthesized by dissolving Li2S together with S in dimethoxyethane, then drying off the solvent, and finally calcining the collected solid. Compared with pure Li2S, such a double-shell material presents a 26.7% improvement in cycling capacity, 0.5 V lower in activation overpotential, and prolonged tolerance in the ambient environment. The density functional theory calculation shows that the performance enhancement lies in the higher stability of Li2S6in contact with moisture and some autocatalytic effect of Li2S2@Li2S6. Such a double-shell structure becomes a universal performanceenhancing approach when being combined with other means, such as cathodes composited with catalytic MoS2, separators modified with selenium-doped sulfurized-polyacrylonitrile/montmorillonite, electrolytes containing fluorenone additive, and Li anodes coated with a layer of Li3N. The corresponding capacity retention shows up to 80% improvement compared with pure Li2S.

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甜菜夜蛾卵黄原蛋白受体基因的RNA干扰

中国农业科学 2019年第1期52卷 56-64页

作者：赵静陶蓉郝德君肖留斌谭永安南京林业大学南方现代林业协同创新中心/林学院江苏省农业科学院植物保护研究所

【目的】卵黄原蛋白受体(vitellogenin receptor,VgR)是介导昆虫卵黄原蛋白胞吞作用的主要受体,通过RNA干扰(RNAi)方法研究甜菜夜蛾(Spodoptera exigua)VgR的功能,为深入了解甜菜夜蛾生殖生理的分子机制及开发有效防治新方法提供依据。... 详细信息

【目的】卵黄原蛋白受体(vitellogenin receptor,VgR)是介导昆虫卵黄原蛋白胞吞作用的主要受体,通过RNA干扰(RNAi)方法研究甜菜夜蛾(Spodoptera exigua)VgR的功能,为深入了解甜菜夜蛾生殖生理的分子机制及开发有效防治新方法提供依据。【方法】以甜菜夜蛾雌成虫腹部的cDNA为模板,通过PCR克隆得到甜菜夜蛾VgR基因片段,其包含配体结合域功能区。绿色荧光蛋白基因(GFP)片段通过特异性引物从笔者实验室保存的GFP质粒上扩增。将VgR和GFP目的片段连入pMD-19T载体后进行测序,利用DNAMAN软件分析基因序列的准确性。以测序验证正确的质粒作为DNA模板,利用带T7启动子的引物进行PCR扩增。用T7 RiboMAXTM Express RNAi System合成试剂盒合成VgR-dsRNA和GFP-dsRNA。应用10μL微量进样器在化蛹第2、6天的甜菜夜蛾雌蛹腹部注射3μL双链RNA(2μg·μL-1)。利用RT-qPCR技术检测甜菜夜蛾刚羽化、羽化24 h、羽化48 h雌成虫的VgR表达量变化,同时统计对照组(空白对照、注射GFP-dsRNA)和处理组(注射VgR-dsRNA)甜菜夜蛾的羽化率及单雌产卵量。【结果】扩增得到VgR和GFP基因片段,大小分别为327和417 bp。RT-qPCR检测结果表明,与对照组相比,注射dsRNA后甜菜夜蛾的VgR表达水平显著下降。对于刚羽化、羽化24 h、羽化48 h的雌成虫,注射VgR-dsRNA处理组的VgR表达量相比注射GFP-dsRNA的对照组分别下降了79.35%、84.22%、67.68%。通过解剖观察刚羽化、羽化24 h、羽化48 h的甜菜夜蛾卵巢,发现注射VgR-dsRNA处理组与注射GFP-dsRNA对照组相比,卵巢发育进度显著推迟。对于羽化24 h的甜菜夜蛾,与注射GFP-dsRNA组相比,注射VgR-dsRNA处理组卵巢管长度下降了23.92%;注射GFP-dsRNA组的卵巢成熟卵粒较多,平均直径为(0.46±0.05)mm,而注射VgR-dsRNA处理组成熟卵粒数量较少且相对较小,平均直径为(0.23±0.02)mm。注射GFP-dsRNA组和VgR-dsRNA组的羽化率无显著差异。注射VgR-dsRNA处理组的单雌平均产卵量只有170粒,而对照组(空白对照和注射GFP-dsRNA)单雌平均产卵量可达到451粒和420粒,处理组与对照组的产卵量存在显著差异。【结论】通过体外注射dsRNA的方法研究VgR的功能,能够显著降低VgR表达。VgR在甜菜夜蛾的生殖中起着不可替代的作用,直接影响甜菜夜蛾卵巢的发育与产卵量,可以作为控制甜菜夜蛾的潜在靶标。

关键词：甜菜夜蛾卵黄原蛋白受体基因 RNA干扰卵巢发育

绿盲蝽G蛋白偶联受体激酶2基因（Al GRK2）的表达分析及在绿盲蝽生长发育中的功能

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中国农业科学 2021年第22期54卷 4813-4825页

作者：谭永安姜义平赵静肖留斌江苏省农业科学院植物保护研究所

【目的】克隆绿盲蝽(Apolygus lucorum)G蛋白偶联受体激酶2基因(AlGRK2)cDNA序列,明确其时空表达谱,阐明外源蜕皮激素(20E)对AlGRK2表达的影响,分析AlGRK2在绿盲蝽生长发育中的作用,为进一步研究其在蜕皮激素信号转导通路中的功能打下... 详细信息

【目的】克隆绿盲蝽(Apolygus lucorum)G蛋白偶联受体激酶2基因(AlGRK2)cDNA序列,明确其时空表达谱,阐明外源蜕皮激素(20E)对AlGRK2表达的影响,分析AlGRK2在绿盲蝽生长发育中的作用,为进一步研究其在蜕皮激素信号转导通路中的功能打下基础。【方法】RACE法克隆获得AlGRK2全长,实时荧光定量PCR(qRT-PCR)分析不同日龄绿盲蝽及雌成虫不同组织中AlGRK2的表达谱,分析外源20E诱导及RNAi处理后,AlGRK2 mRNA表达的应答反应及对绿盲蝽生长发育主要参数(发育历期、若虫体重及成虫羽化率)的影响。【结果】AlGRK2 cDNA序列全长2 715 bp,开放阅读框2 106 bp,编码701个氨基酸,ExPASy预测其蛋白分子量为80.2 kD,理论等电点为6.56;蛋白结构分析显示AlGRK2包含4个结构域,即G蛋白信号调节区(RGS,54—175 aa)、丝氨酸/苏氨酸激酶结构域(S-TKc,191—454 aa)、丝氨酸/苏氨酸型蛋白激酶的伸展部分(S-TK-X,455—534 aa)和PH结构域(PH,558—655 aa),其中PH结构域是GRK2蛋白的典型结构域;系统发育分析结果表明,绿盲蝽GRK2与茶翅蝽GRK2亲缘关系最近;AlGRK2在绿盲蝽1—16日龄虫体内均有表达,mRNA表达量呈现出波动式下降的模式,在绿盲蝽初始龄期的表达量较高,而在末龄期的表达量显著下降;AlGRK2在绿盲蝽雌成虫卵巢和脂肪体中高表达,在胸与足中的表达量较低;外源20E处理后,AlGRK2在绿盲蝽1日龄和3日龄表达量显著下调,AlGRK2在雌成虫各组织中均表达上调,在卵巢及脂肪体中上调幅度最大,相反的是,20E信号通路中PLC抑制剂U73122处理的AlGRK2表达量下调;绿盲蝽若虫发育历期、末龄若虫体重和成虫羽化率均显著下降,相反的是,U73122处理组若虫期的发育历期显著延长;此外,与注射ds GFP处理组相比,注射ds AlGRK2处理后绿盲蝽的AlGRK2表达水平显著下降,若虫死亡率及发育历期显著增加,而成虫羽化率和5龄若虫体重均显著下降。【结论】AlGRK2在绿盲蝽体内的表达谱显示出发育阶段特异性和组织特异性;外源20E抑制剂及RNAi处理后,均可抑制AlGRK2的表达,同时还可对绿盲蝽生长发育产生不利影响,表现为延缓绿盲蝽的发育进度、降低5龄若虫体重及成虫羽化率。

关键词：绿盲蝽 G蛋白偶联受体激酶2 蜕皮激素基因克隆表达谱 RNA干扰

从导学一体到数智嵌入:研究生思想政治教育引导力的时代转向

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学位与研究生教育 2024年第7期 26-33页

作者：汪永安孙增耀东华大学人文学院上海201620 东华大学研究生院学位办公室上海201620

数智技术的快速发展和数字网络社会的发展对意识形态安全提出了更高要求,推动着研究生思想政治教育守正创新。导学一体模式下的研究生导学思政模式正面临网络多元接触弱化导师引导地位、交互模式诱发导生关系异化、时空跨越消解主流价... 详细信息

数智技术的快速发展和数字网络社会的发展对意识形态安全提出了更高要求,推动着研究生思想政治教育守正创新。导学一体模式下的研究生导学思政模式正面临网络多元接触弱化导师引导地位、交互模式诱发导生关系异化、时空跨越消解主流价值引力等现实困境,而教育数智化转型对于思想政治教育的影响是全方位的,赋予研究生思想政治教育以数据融通、分众传播、虚实互嵌等创新共生源动力。新时代研究生思想政治教育应以研究生为中心,树立全域、全时、全员融合的教育理念,从研究生思想政治教育工作的贴源层、清洗层、融合层、共享层夯实底层数字基座,通过顶层设计、提升素养、搭建平台激发主体参与动力,推动研究生思想政治教育工作评价体系改革,切实提高立德树人的针对性和实效性。

关键词：数智赋能思想政治教育导学思政数智技术研究生教育