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Protective effect of L-arginine preconditioning on ischemia and reperfusion injury associated with rat small bowel transplantation

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World Journal of Gastroenterology 2005年第19期11卷 2994-2997页

作者： BinCao NingLi yongwang Jie-ShouLi DepartmentofSurgery NanjingUniversityMedicalSchoolTheFirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029JiangsuProvinceChina DepartmentofSurgery NanjingUniversityMedicalSchoolNanjing210002JiangsuProvinceChina

AIM: To investigate the protective effect and possible mechanism of L-arginine preconditioning on ischemia and reperfusion injury associated with small bowel transplantation (SBT).METHODS: Male inbred Wistar rats weighting between 180 and 250 g were used as donors and recipients in thestudy. Heterotopic rat SBT was performed according to the techniques of Li and Wu. During the experiment, intestinal grafts were preserved in 4 ℃ Ringer's solution for 8 h before being transplanted. Animals were divided into three groups. In group 1, donors received intravenous L-arginine (50 mg/kg, 1 mL) injection 90 min before graft harvesting. However, donors in control group were given normal saline (NS) instead. In group 3, six rats were used as sham-operated control. Specimens were taken from intestinal grafts 15 min after reperfusion. Histological grading, tissue malondialdehyde (MDA) and myeloperoxidase (MPO) levels were assessed. The graft survival of each group was monitored daily until 14 d after transplantation. RESULTS: Levels of MDA and MPO in intestine of shamoperated rats were 2.0±0.22 mmol/g and 0.66±0.105 U/g. Eight hours of cold preservation followed by 15 min of reperfusion resulted in significant increases in tissue MDA and MPO levels. Pretreatment with L-arginine before graft harvesting resulted in lower enhancement of tissue levels of MDA and MPO and the differences were significant (4.71±1.02 mmol/g vs8.02±3.49 mmol/g, 1.03±0.095 U/g vs 1.53±0.068 U/g, P＜0.05). Besides, animals in L-arginine pretreated group had better histological structures and higher 2-wk graft survival rates comparing with that in NS treated group (3.3±0.52 vs6±0.1, 0/6 vs6/6, P＜0.05or 0.01).CONCLUSION: L-arginine preconditioning attenuates ischemia and reperfusion injury in the rat SBT model,which was due to antioxidant activities partially.

关键词： L-精氨酸保护机制预处理小肠移植缺血再灌注损伤

Expression and significance of Rap1A in testes ofazoospermic subjects

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Asian Journal of Andrology 2004年第1期6卷 35-40页

作者： BoYang HeWang Xiao-KangGao Bao-QiChen Yuan-QiangZhang He-LiangLiu yongwang Wei-JunQin Rong-LiangQin Guo-XingShao ChenShao DepartmentofUrology XijingHospital DepartmentofHistologyandEmbryology FourthMilitaryMedicalUniversityXi''an710033China

Aim: To evaluate the Rap1A mRNA expression and its significance in the testes of normal and azoospermic subjects. Methods: A cDNA microarray that contained Rap1A and some other genes such as RBM, EIF1 AY was used to identify the differential gene expression profiles between the normal and azoospermic testes. cDNA probes were prepared by labeling mRNA from azoospermic and normal testicular tissues through reverse transcription with Cy5-dUTP and Cy3-dUTP, respectively. The mixed cDNA probes were then hybridized with cDNA microarray (each containing 4096 unique human cDNA sequences). The fluorescent signals were scanned and the values of Cy5-dUTP and Cy3-dUTP on each spot were analyzed and calculated. In situ hybridization was employed to detect the expression of RaplA in the testes of 10 fertile and 39 azoospermic subjects. Results: One hundred and twenty-eight differentially expressed genes were found to be possibly related to azoospermia, of which 56 were up-regulated and 72, down-regulated genes. The mRNA expression of RaplA in the spermatogenic cells of azoospermic was stronger than that in those of the fertile testes. Conclusion: Rap1A may play certain roles in the development of azoospermia.

关键词： Rap1A azoospermia cDNA microarray

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即时凝血检测用于心血管手术的研究进展

中华麻醉学杂志 2024年第3期44卷 380-384页

作者：王永旺冉菊红彭勇刚桂林医学院附属医院麻醉科桂林541001 郑州大学第一附属医院麻醉与围术期医学部郑州450052 佛罗里达大学Shands医院麻醉与围术期医学部盖恩斯维尔32610佛罗里达美国

近年来接受心血管手术的人数呈逐年增长趋势,2021年全国心血管外科手术量近28万例,比2020年增加了25%[1]。心血管手术术式复杂、术前抗凝和/或抗血小板等调节出凝血药物的使用及体外循环的影响(血液稀释、凝血因子消耗、血小板损伤、纤... 详细信息

近年来接受心血管手术的人数呈逐年增长趋势,2021年全国心血管外科手术量近28万例,比2020年增加了25%[1]。心血管手术术式复杂、术前抗凝和/或抗血小板等调节出凝血药物的使用及体外循环的影响(血液稀释、凝血因子消耗、血小板损伤、纤溶亢进、肝素的不完全逆转及肝素反弹等)[2],可能是围术期大量失血和凝血功能障碍的主要因素。目前,围术期出凝血管理仍是一个挑战,麻醉医生对于围术期出凝血监测,需要迎难而上,即时准确地找出围术期出凝血功能障碍的原因,为患者平稳麻醉提供保障。而传统凝血功能检查耗费时间长,仅反映凝血过程的某个阶段,无法准确判断异常出血原因,以及影响凝血功能障碍的具体阶段和因子成分,且围术期出凝血是动态过程,未及时判断原因可能导致延迟或不适当的治疗。即时凝血检测(又称床旁凝血检测)可部分弥补传统凝血功能检查的局限性,快速识别凝血功能障碍,并实施有的放矢的靶向治疗。本文就即时凝血检测用于心血管手术的研究进展予以综述,以期为心血管手术患者围术期出凝血管理提供参考。

关键词：凝血功能障碍凝血检测血液稀释异常出血心血管手术凝血过程手术术式纤溶亢进

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基于BERT的多视角事件日志修复

计算机应用研究 2024年第2期41卷 515-520页

作者：张振虎王丽丽袁永旺安徽理工大学数学与大数据学院安徽淮南232001 安徽理工大学深部煤矿采动响应与灾害防控国家重点实验室安徽淮南232001 安徽理工大学安徽省煤矿安全大数据分析与预警技术工程实验室安徽淮南232001

在业务流程执行过程中,由于信息系统故障或者人工记录出错等问题导致事件日志中数据的丢失,从而产生缺失的事件日志,使用这种缺失日志会严重影响业务流程分析结果的质量。针对这种缺失日志的修复问题,现有研究大部分仅从数据视角或者行... 详细信息

在业务流程执行过程中,由于信息系统故障或者人工记录出错等问题导致事件日志中数据的丢失,从而产生缺失的事件日志,使用这种缺失日志会严重影响业务流程分析结果的质量。针对这种缺失日志的修复问题,现有研究大部分仅从数据视角或者行为视角进行展开,很少从数据和行为相融合的视角开展事件日志的修复工作。提出了一种基于BERT模型的多视角事件日志修复方法。该方法利用双层BERT模型,从数据和行为融合的视角训练模型,通过BERT模型的预训练任务((masked attribute model,MAM)和(masked event model,MEM))以及Transformer编码块的注意力机制捕获输入属性的双向语义信息和长期依赖关系,使用微调策略进行模型训练,以预测的形式修复事件日志中的缺失值。最后,通过公开可用的数据集进行评估分析,结果表明,该方法在修复事件日志方面表现良好。

关键词：缺失日志数据和行为融合多视角修复双层BERT 微调策略

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自驱修孔钻头动力参数及钻齿结构研究

煤田地质与勘探 2024年第4期52卷 179-189页

作者：刘勇李阳魏建平苑永旺代硕李林豫河南理工大学瓦斯地质与瓦斯治理国家重点实验室培育基地河南焦作454000 河南理工大学煤炭安全生产与清洁高效利用省部共建协同创新中心河南焦作454000

自驱修孔钻头是修复失效长钻孔的有效技术手段,为进一步提高修孔速度和瓦斯抽采效果,本文基于射流反冲理论,分析了射流反冲力与喷嘴参数的关系,构建了自驱修孔钻头旋转力学理论模型。采用LS-DYNA开展不同结构钻齿破煤过程,分析了不同钻... 详细信息

自驱修孔钻头是修复失效长钻孔的有效技术手段,为进一步提高修孔速度和瓦斯抽采效果,本文基于射流反冲理论,分析了射流反冲力与喷嘴参数的关系,构建了自驱修孔钻头旋转力学理论模型。采用LS-DYNA开展不同结构钻齿破煤过程,分析了不同钻进压力和扭矩作用下钻齿对煤体破坏形式和钻齿破煤体积变化规律,并开展了破煤实验验证数值模拟结果。形成了自驱修孔钻头参数确定方法,设计了自驱修孔钻头,并在郑煤集团超化煤矿22煤柱面底抽巷进行了工程实验。研究结果表明:(1)喷嘴张角α和偏心角β是决定钻进压力和扭矩分配的关键参数,通过调控张角和偏心角可实现钻进压力和扭矩的最优匹配。(2)相同钻进参数条件下,钻齿结构对破煤体积影响较大,阶梯钻齿破煤效果最优。在钻进压力为120N、扭矩0.6N·m时,阶梯型钻齿结构的修孔钻头能够实现钻进压力和扭矩最优匹配。(3)确定了最优修孔钻头结构参数为:钻头外径28 mm;后置喷嘴张角20°,偏心角90°;前置喷嘴张角90°,偏心角0°。(4)工程应用结果表明:瓦斯抽采纯量提高了1.96倍,瓦斯抽采体积分数提高了3.98倍,修孔速度提高了1.2倍。通过改进钻头动力参数及优化钻齿结构,设计的自驱修孔钻头在工程实践中达到了提高修孔速度和瓦斯抽采效果的目的。

关键词：瓦斯抽采钻孔修复钻进压力钻齿结构煤矿

大豆NRT2家族基因的鉴定、特征及表达模式

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分子植物育种 2024年第13期22卷 4185-4191页

作者：隋超孙永旺郭尚敬聊城大学农学院聊城252000

硝酸盐转运蛋白2 (Nitrate transporter 2, NRT2)是一种高亲和硝酸盐转运蛋白,在许多植物对硝酸盐的吸收和转运过程中发挥重要作用。本研究旨在对大豆NRT2基因家族进行全基因组鉴定和生物信息学分析。结果表明,大豆中有6个NRT2基因,分布... 详细信息

硝酸盐转运蛋白2 (Nitrate transporter 2, NRT2)是一种高亲和硝酸盐转运蛋白,在许多植物对硝酸盐的吸收和转运过程中发挥重要作用。本研究旨在对大豆NRT2基因家族进行全基因组鉴定和生物信息学分析。结果表明,大豆中有6个NRT2基因,分布在5条染色体上。根据进化关系可将大豆NRT2家族基因分为两类,每类中的基因结构和保守结构域分布相似。GmNRT2启动子区域均含有大量的光响应元件,这表明GmNRT2基因的表达可能受光信号调控。表达模式分析发现,大豆NRT2家族基因主要在根和根毛中表达,表明该家族基因与氮素吸收可能有着紧密的关系。本研究结果为大豆NRT2家族基因生物学功能研究提供了一定基础。

关键词：大豆 NRT2基因家族硝酸盐氮吸收

Effects of endostatin-vascular endothelial growth inhibitor chimeric recombinant adenoviruses on antiangiogenesis

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World Journal of Gastroenterology 2004年第10期10卷 1409-1414页

作者： XinPan yongwang MinZhang WeiPan Zhong-TianQi Guang-WenCao DepartmentofMicrobiology SecondMilitaryMedicalUniversityShanghai200433China DepartmentofOphthalmologyofChanghaiHospital SecondMilitaryMedicalUniversityShanghai200433China DepartmentofEpidemiology SecondMilitaryMedicalUniversityShanghai200433China

AIM: To investigate the inhibitory effects of endostatinvascular endothelial growth inhibitor (VEGI151) recombinant adenoviruses on neovascularization.METHODS: We used recombinant adenoviruses to treat human vascular endothelial cell line ECV304, human hepatocellular carcinoma cell line HepG2, and murine fibroblast cell line L929, in order to study the chimeric gene expression in these cell lines. Chick choriallantic membrane(CAM) model, rabbit inflammatory corneal neovascularization(CNV) model, and liver cancer-bearing nude mice model were employed to investigate the negative biological effect of fusion molecules on neovascularization in vivo.RESULTS: Western blot showed that the molecular weight of fusion protein was about 41kD after infection of ECV304,HepG2 and L929 cells with supernatant of AdhENDO-VEGI151.The fusion protein showed a specific inhibitory effect on the proliferation of ECV304 cells, but no inhibitory effect on the growth of HepG2 and L929 cells (F=13112.13, P=0.0001). In the chick choriallantic membrane (CAM) assay, the expressed fusion protein significantly inhibited neovascularization. Rabbit inflammatory corneal neovascularization (CNV) induced by intrastromal sutures resulted in a uniform neovascular response. In this model, direct subconjunctival injection of AdhENDO-VEGI151 expressed the fusion protein in vivo and suppressed the development of CNV. Topical application of AdhENDO-VEGI151 led to a significant suppression of CNV (F=1413.11, P=0.0001),as compared with the control group of AdLacZ. Immunohistochemical staining showed the fusion protein dominantly expressed in corneal epithelium. Compared with the control group of AdLacZ (4075.9±1849.9 mm^3), the average tumor size of group AdhENDO-VEGI151 reduced in size (487.7±241.2 mm^3) (F=14.80, P=0.0085), with an inhibition rate of 88.03%. Immunohistochemical staining showed the adenoviruses carried the fusion gene expressed on liver cancer cell membrane. MVD decreased more significantly in treated mice (30.75±3.31%) than in AdLacZ contro

关键词：血管内皮生长因子重组腺病毒抗肿瘤药微血管形成免疫印迹法

Distribution Patterns of Calcite Cement in the Turbidite Sandstones of the Shahejie Formation in the Dongying Depression,Eastern China

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Acta Geologica Sinica(English Edition) 2022年第3期96卷 1041-1056页

作者： ZHANG yongwang QU Zhengyang State Key Laboratory of Petroleum Resources and Prospecting Beijing 102249China College of Geosciences China University of Petroleum-BeijingBeijing 102249China No.4 Oil Production Plant PetroChina Changqing Oilfield CompanyYinchuan 718500China

Calcite cements are volumetrically dominant among the most diagenetic constituents in turbidite sandstones of the Paleogene Shahejie Formation in the Dongying Depression.The results show carbonate cements mainly occur in three phases:Calcite I(pre-compaction),Calcite II(post-compaction)and ankerite in the turbidite sandstone.The isotopic composition of Calcite I and Calcite II range from−4.3‰to 4.4‰,−3.5‰to 4.3‰PDB,respectively,suggesting that the calcite cements are mainly derived from the interbedded shales.The early calcite cement precipitated from the mixing of lacustrine and meteoric waters at 38-69℃.The late-phase calcite precipitated from pore waters modified by water-rock interactions at 64-126℃.Two distribution patterns of calcite cements can be identified,depending on their position within the sandstone.The peripheral cementation of the sandstones is near the sand-shale contact.The development of cement is controlled by sandstone thickness and the properties of the interbedded shale.For scattered cementation,the distribution of the calcite cement is not controlled by the sand-shale contact.Calcite cement was preferentially distributed in coarse-grained sandstones,indicating that sandstones with high original permeability are the preferred pathway for the migration of early diagenetic fluids.

关键词： turbidite sandstone calcite cement distribution patterns Dongying Depression

Decision-making Impairments in Primary Angle-closure Glaucoma Patients

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Chinese Medical Journal 2017年第12期130卷 1424-1428页

作者： Hui Pu yongwang Qiang Wei Hui-Juan Ma Pan-Pan Hu Shou-Ling Li Hong-Bo Pang Yang-Hua Tian Kai Wang Department of Neurology The Second People's Hospital of Wuhu Wuhu Anhui 241000 China Department of Neurology The First Hospital of Anhui Medical University Hefei Anhui 230032 China Anhui Province Key Laboratory of Cognition and Neuropsychiatric Disorders Hefei Anhui 230032 China Department of Ophthalmology The First Hospital of Anhui Medical University Hefei Anhui 230032 China

Background： Primary angle-closure glaucoma （PACG） is a common eye disease and a common cause of blindness. Inappropriate medical decisions severely affect the prognosis. This study investigated decision-making under risk in PACG patients.Methods： Thirty patients with first acute attack of PACG before surgery and thirty healthy controls were included in the study. Decision-making under risk was evaluated with the game of dice task （GDT）. The results of Eysenck Personality Questionnaire （EPQ） and GDT between PACG patients and healthy controls were compared. Results： Risky decisions in PACG patients were more than those in healthy controls as measured by mean score ofGDT （ 12.47 ±5.72 vs. 4.33± 3.30, P 〈 0.001）. Higher neuroticism score in EPQ was found in PACG patients compared to healthy controls （14.97 ± 3.93 vs. 9.90 ± 4.49, P 〈 0.001 ）. Neuroticism scores in EPQ were associated with decision-making performance （r = 0.417, P = 0.001 ）. Conclusions： Neuroticism positively correlated with risky decisions. Decision-making might be influenced by neuroticism. Future studies will show whether therapy compliance will be improved by emotional management and psychological intervention in PACG patients.

关键词： Cognitive Decision-making： Neuroticism Personality Primary Angle-closure Glaucoma