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Effects of herbicide butachlor application on the growth of periphytic biofilms and nitrogen loss in paddy systems

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Pedosphere 2024年第1期34卷 211-221页

作者： Yanhui zhao Mingjun LEI Liandong JING Fan XIA Meixia YAN Jiantong LIU yonghong WU Yunli WU Chenxi WU Ecology and Environment Monitoring and Scientific Research Center Yangtze Basin Ecology and Environment AdministrationMinistry of Ecological and EnvironmentWuhan 430010China College of Chemistry and Environmental Protection Engineering Southwest Minzu UniversityChengdu 610041China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil SciencesChinese Academy of SciencesNanjing 210008China

The application of butachlor as an herbicide in paddy fields is widely practiced,aiming to increase rice yield by directly or indirectly influencing the paddy environment.Periphytic biofilms,which form at the soil-water interface in paddy fields,are complex bioaggregates that play an important role in nitrogen (N) cycling.The objective of this study was to investigate the effect of butachlor on periphytic biofilm growth and N cycling under both light and dark conditions in the laboratory.The results revealed that butachlor application hindered the growth of periphytic biofilms and led to the dominance of Cyanobacteria as the primary prokaryotes,while inhibiting the development of eukaryotic Trebouxiophyceae.Furthermore,the application of butachlor reduced the richness and diversity of prokaryotes,but increased those of eukaryotes in periphytic biofilms.The light treatments exhibited higher total N loss because light favored periphytic biofilm growth and enhanced ammonium (NH_(4)^(+)) assimilation and nitrification.Additionally,butachlor application resulted in the increased retention of NH_(4)^(+)-N and nitrate (NO_(3)^(-))-N and an increase in N loss via denitrification.The abundances of functional genes encoding enzymes such as ammonia monooxygenase,nitrite reductase,and nitrous oxide reductase were increased by butachlor application,favoring nitrification and denitrification processes.Overall,the results suggest that butachlor application leads to an increase in total N loss mainly through denitrification in paddy systems,particularly in the presence of periphytic biofilms.Thus,the results may provide valuable insights into the changes in periphytic biofilm growth and N cycling induced by butachlor,and future studies can further explore the potential implications of these changes in paddy soils.

关键词： ammonia-oxidizing bacteria amoA denitrification denitrifying bacteria nirK nitrification nitrogen cycling nosZ volatilization

A unique subseafloor microbiosphere in the Mariana Trench driven by episodic sedimentation

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Marine Life Science & Technology 2024年第1期6卷 168-181页

作者： Jiwen Liu Da-Wei Li Xinxin He Ronghua Liu Haojin Cheng Chenglong Su Mengna Chen yonghong Wang Zhongsheng zhao Hanyue Xu Zhangyu Cheng Zicheng Wang Nikolai Pedentchouk David J.Lea-Smith Jonathan D.Todd Xiaoshou Liu Meixun zhao Xiao-Hua Zhang Frontiers Science Center for Deep Ocean Multispheres and Earth System and College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Ecology and Environmental Science Laoshan LaboratoryQingdao 266237China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationOcean University of ChinaQingdao 266100China Key Lab of Submarine Geosciences and Prospecting Techniques Ministry of Education/College of Marine GeosciencesOcean University of ChinaQingdao 266100China Key Laboratory of Physical Oceanography Ministry of Education/Research Vessel CentreOcean University of ChinaQingdao 266100China School of Environmental Sciences University of East AngliaNorwich NR47TJUK School of Biological Sciences University of East AngliaNorwich NR47TJUK

Hadal trenches are characterized by enhanced and infrequent high-rate episodic sedimentation events that likely introduce not only labile organic carbon and key nutrients but also new microbes that significantly alter the subseafloor microbiosphere.Currently,the role of high-rate episodic sedimentation in controlling the composition of the hadal subseafloor microbiosphere is unknown.Here,analyses of carbon isotope composition in a~750 cm long sediment core from the Challenger Deep revealed noncontinuous deposition,with anomalous ^(14)C ages likely caused by seismically driven mass transport and the funneling effect of trench geomorphology.Microbial community composition and diverse enzyme activities in the upper~27 cm differed from those at lower depths,probably due to sudden sediment deposition and differences in redox condition and organic matter availability.At lower depths,microbial population numbers,and composition remained relatively constant,except at some discrete depths with altered enzyme activity and microbial phyla abundance,possibly due to additional sudden sedimentation events of different magnitude.Evidence is provided of a unique role for high-rate episodic sedimentation events in controlling the subsurface microbiosphere in Earth’s deepest ocean floor and highlight the need to perform thorough analysis over a large depth range to characterize hadal benthic populations.Such depositional processes are likely crucial in shaping deep-water geochemical environments and thereby the deep subseafloor biosphere.

关键词： Hadal subseafloor Deep water sediment Mariana Trench Radiocarbon Microbial community Redox potential

Superior effectiveness and acceptability of saliva samples for the detection of SARS-CoV-2 in China

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Biosafety and Health 2024年第2期6卷 88-91页

作者： Hui Yao Ying Shen Zhichao Liang Xiaoyu Xue Chenxi zhao Xiang Xu Yuxin Cai yonghong Liu Wei Zhang Yang Pan Xiaoli Wang Bejing Office of Global Health Beijing Center for Disease Prevention and ControlBejing 100013China Institute for Infectious Disease and Endemic Disease Control Beijing Center for Disease Prevention and ControlBejing 100013China National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Bejing Ditan HospitalCapital Medical UniversityBejing 100015China Center for Drug Evaluation National Medical Products AdministrationBejing 100176China School of Public Health Capital Medical UniversityBeijing 100069China

In response to problems of poor sampling quality,low sensitivity,and high demand for medical personnel regarding the current severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)oropharyngeal(OP)swab sampling used in China,we aimed to evaluate the diagnostic performance and acceptability of saliva-based nucleic acid amplification tests(NAATs)in China.The results showed that,using nasopharyngeal(NP)swab results as the gold standard,the overall sensitivities for saliva specimens and OP swabs were 93.3%and 85.0%,the specificities were 92.6%and 93.8%,respectively.The results of an acceptability survey showed that the scores for saliva,OP,and NP samples were 9.46±1.69,8.11±2.42,and 4.58±3.82 out of 10,respectively,with significant differences among the three groups(P

关键词： SARS-CoV-2 Saliva sample Sensitivity Acceptability

Experimental study on strain field evolution around a simulated thrust fault

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Earthquake Science 2023年第1期36卷 40-51页

作者： yonghong zhao Yanjun Xiao Jiaying Yang Xiaofan Li Andong Xu School of Earth and Space Sciences Peking UniversityBeijing 100871China State Key Laboratory of Shale Oil and Gas Enrichment Mechanisms and Effective Development Beijing 100083China SinoPEC Key Laboratory of Seismic Elastic Wave Technology Beijing 100083China SinoPEC Petroleum Exploration and Production Research Institute Beijing 100083China

Earthquakes result from continuous geodynamic processes.A topic of significant interest for the scientific community is to elaborate on the phenomena governing the faulting and fracturing of crustal rocks.Therefore,in this study,uniaxial compressive shear failure experiments were conducted on Fangshan marble rock samples with a prefabricated slot to simulate thrust faulting.The center of each marble plate(105 mm × 80 mm × 5 mm) was engraved with a 30-mm long double-sided nonpenetrating slot(depth:2 mm,width:0.5 mm).The deformation and destruction processes of the rock surface were recorded using a high-speed camera.The digital image correlation method was used to calculate the displacement and strain distribution and variation at different loading stages.The accumulative and incremental displacement fields u and v,strain field exand e_(y),and shear strain e_(xy) were analyzed.When the loading level reached its ultimate value,the strain field was concentrated around the prefabricated slot.The concentration reached a maximum at the ends of the prefabricated slot.The magnitude of shear strain reached 0.1.This experiment contributes to our understanding of the dynamic process of active faulting.

关键词： experimental study deformation field evolution fracture process earthquake

Virtual source approach for maximizing resolution in high-penetration gamma-ray imaging

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Matter and Radiation at Extremes 2024年第3期9卷 19-30页

作者： Yuchi Wu Shaoyi Wang Bin Zhu yonghong Yan Minghai Yu Gang Li Xiaohui Zhang Yue Yang Fang Tan Feng Lu Bi Bi Xiaoqin Mao Zhonghai Wang Zongqing zhao Jingqin Su Weimin Zhou Yuqiu Gu National Key Laboratory of Plasma Physics Laser Fusion Research CenterCAEPMianyangSichuan 621900China College of Physics Sichuan UniversityChengdu 610065China

High-energy gamma-ray radiography has exceptional penetration ability and has become an indispensable nondestructive testing(NDT)tool in various fields.For high-energy photons,point projection radiography is almost the only feasible imaging method,and its spatial resolution is primarily constrained by the size of the gamma-ray source.In conventional industrial applications,gamma-ray sources are commonly based on electron beams driven by accelerators,utilizing the process of bremsstrahlung radiation.The size of the gamma-ray source is dependent on the dimensional characteristics of the electron beam.Extensive research has been conducted on various advanced accelerator technologies that have the potential to greatly improve spatial resolution in NDT.In our investigation of laser-driven gamma-ray sources,a spatial resolution of about 90μm is achieved when the areal density of the penetrated object is 120 g/cm^(2).A virtual source approach is proposed to optimize the size of the gamma-ray source used for imaging,with the aim of maximizing spatial resolution.In this virtual source approach,the gamma ray can be considered as being emitted from a virtual source within the convertor,where the equivalent gamma-ray source size in imaging is much smaller than the actual emission area.On the basis of Monte Carlo simulations,we derive a set of evaluation formulas for virtual source scale and gamma-ray emission angle.Under optimal conditions,the virtual source size can be as small as 15μm,which can significantly improve the spatial resolution of high-penetration imaging to less than 50μm.

关键词： resolution approach utilizing

Tet methylcytosine dioxygenase 2(TET2)deficiency elicits EGFR-TKI(tyrosine kinase inhibitors)resistance in non-small cell lung cancer

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1706-1720页

作者： Jian Zhang Kejia zhao Wenjing Zhou Ran Kang Shiyou Wei Yueli Shu Cheng Yu Yin Ku yonghong Mao Hao Luo Juqin Yang Jiandong Mei Qiang Pu Senyi Deng Zhengyu Zha Gang Yuan Shensi Shen Yaohui Chen Lunxu Liu Department of Thoracic Surgery and Institute of Thoracic Oncology West China Hospital of Sichuan UniversityChengdu 610097China Western China Collaborative Innovation Center for Early Diagnosis and Multidisciplinary Therapy of Lung Cancer Sichuan UniversityChengdu 610097China Biobank of West China Hospital Sichuan UniversityChengdu 610041China

Despite epidermal growth factor receptor(EGFR)tyrosine kinase inhibitors(TKl)have shown remarkable efficacy in patients with EGFR-mutant non-small cell lung cancer(NSCLC),acquired resistance inevitably develops,limiting clinical efficacy.We found that TET2 was poly-ubiquitinated by E3 ligase CUL7^(FBXW11) and degraded in EGFR-TKI resistant NSCLC ells.Genetic perturbationof TET2 rendered parental cells more tolerant to TKI treatment.TET2 was stabilized by MEK1 phosphorylation at Ser 1107,while MEK1 inactivation promoted its proteasome degradation by enhancing the recruitment of CUL7^(FBXW11),Loss of TET2 resulted in the upregulation of TNF/NF-kB signaling that confers the EGFR-TKI resistance.Genetic or pharmacological inhibition of NF-kB attenuate the TKI resistance both in vitro and in vivo.Our findings exemplified how a cell growth controlling kinase MEK1 leveraged the epigenetic homeostasis by regulating TET2,and demonstrated an alternative path of non-mutational acquired EGFR-TKI resistance modulated by TET2 deficiency.Therefore,combined strategy exploiting EGFR-TKI and inhibitors of TET2/NF-κB axis holds therapeutic potential for treating NSCLC patients who suffered from this resistance.

关键词： TET2 TKI resistance

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中国基本养老服务制度的伦理向度

中州学刊 2024年第5期 110-117页

作者：庞永红杨钊重庆大学马克思主义学院重庆400044

基本养老服务制度关乎伦理,蕴含遵循以人民为中心的核心价值、彰显共建共享的顶层设计、聚焦正义和效率的协调统一、秉持公共善和个体善相辅相成的伦理属性;在制度的执行和贯彻中存在保基本与需求多样性的差距、传统代际养老责任意识与... 详细信息

基本养老服务制度关乎伦理,蕴含遵循以人民为中心的核心价值、彰显共建共享的顶层设计、聚焦正义和效率的协调统一、秉持公共善和个体善相辅相成的伦理属性;在制度的执行和贯彻中存在保基本与需求多样性的差距、传统代际养老责任意识与普惠性相背离、资源配置的均衡性不足等方面的实然伦理困境;只有聚焦基本养老服务责任的顺序性和协同性相结合、传统孝老爱亲文化的创造性转化和创新性发展、基本养老服务分配形式公平和实质公平相统一等应然伦理路径,基本养老服务制度建设才能在“善”的应然方向彰显更大效能。

关键词：基本养老服务制度伦理属性伦理困境伦理路径

Dislocation Creep Accommodated by Grain Boundary Sliding in Dunite

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Journal of Earth Science 2010年第5期21卷 541-554页

作者： yonghong zhao David L Kohlstedt School of Earth and Space Sciences Peking University Department of Geology and Geophysics University of Minnesota

To investigate the role of grain boundary sliding during dislocation creep of dunite, a series of deformation experiments were carried out under anhydrous conditions on fine-grained （-15 μm） samples synthesized from powdered San Carlos olivine and powdered San Carlos olivine＋1.5 vol.% MORB. Triaxial compressive creep tests were conducted at a temperature of 1 473 K and confining pressures of 200 and 400 MPa using a high-resolution, gas-medium deformation apparatus. Each sample was deformed at several levels of differential stress between 100 and 250 MPa to yield strain rates in the range of 10^-6 to 10^-4 s^-1. Under these conditions, the dominant creep mechanism involves the motion of dislocations, largely on the easy slip system （010）[100], accommodated by grain boundary sliding （gbs）. This grain size-sensitive creep regime is characterized by a stress exponent of n=3.4±0.2 and a grain size exponent of p=2.0±0.2. The activation volume for this gbs-accommodated dislocation creep regime is V＊=（26±3）×10^-6 m2·mol^-1. Comparison of our flow law for gbs-accommodated dislocation creep with those for diffusion creep and for dislocation creep reveals that the present flow law is important for the flow of mantle rocks with grain sizes of 〈100μm at differential stresses 〉20 MPa. Hence, gbs-accommodated dislocation creep is likely to be an important deformation mechanism in deep-rooted, highly localized shear zones in the lithospheric upper mantle.

关键词： grain boundary sliding creep olivine flow law.

VDT作业光环境对不同体质设计师视健康的影响

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西部人居环境学刊 2024年第1期39卷 142-148页

作者：喻泉严永红赵品勇路瑜重庆大学建筑城规学院重庆大学山地城镇建设与新技术教育部重点实验室重庆大学数学与统计学院重庆市中医院

为探究光环境、体质与视健康的交互作用关系,保护高风险体质者视觉作业安全。首先,对重庆地区9家建筑勘察设计企业进行光环境调研,测量72间视觉显示终端(VDT)办公室的水平作业面照度、相关色温、眼位照度等光环境参数,计算节律照明相关... 详细信息

为探究光环境、体质与视健康的交互作用关系,保护高风险体质者视觉作业安全。首先,对重庆地区9家建筑勘察设计企业进行光环境调研,测量72间视觉显示终端(VDT)办公室的水平作业面照度、相关色温、眼位照度等光环境参数,计算节律照明相关指标,并与标准进行比对;然后,对541名青年设计师进行中医体质辨识及视疲劳特征记录;最后,基于中医体病相关理论,分析环境光照下体质与近视、视疲劳的相关性,辨别视健康高风险体质。结果表明:青年设计师的体质类型以平和质为主,在9种体质中占比最高;偏颇体质中阳虚质占比最高,对近视、视疲劳的易感性显著高于平和质;工作场所长期日光暴露不足、节律刺激过低易诱发体质向阳虚质转变,增加视健康风险。

关键词：作业光环境视健康视觉显示终端中医体质个体差异