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Adipose-derived stem cells modified by BDNF gene rescue erectile dysfunction after cavernous nerve injury

Neural Regeneration Research 2020年第1期15卷 120-127页

作者： Mei Yang Jiang-Yang Sun Cheng-Cheng Ying yong Wang yong-lian guo Department of Endocrinology Tongren Hospital of Wuhan University(Wuhan Third Hospital)WuhanHubei ProvinceChina Department of Hepatobiliary Surgery The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Urology The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Cavernous nerve injury is the main cause of erectile dysfunction following radical *** recovery of erectile function following radical prostatectomy remains *** previous studies found that injecting adipose-derived stem cells(ADSCs)into the cavernosa could repair the damaged cavernous nerves,but the erectile function of the treated rats could not be restored to a normal *** evaluated the efficacy of ADSCs infected with a lentiviral vector encoding rat brain-derived neurotrophic factor(lenti-rBDNF)in a rat model of cavernous nerve *** rats were equally and randomly divided into four *** the control group,bilateral cavernous nerves were isolated but not *** the bilateral cavernous nerve injury group,bilateral cavernous nerves were isolated and injured with a hemostat clamp for 2 *** the ADSCGFP and ADSCrBDNF groups,after injury with a hemostat clamp for 2 minutes,rats were injected with ADSCs infected with lenti-GFP(1×106 in 20μL)and lenti-rBDNF(1×106 in 20μL),*** function was assessed 4 weeks after injury by measuring intracavernosal ***,penile tissues were collected for histological detection and western blot *** demonstrated that compared with the bilateral cavernous nerve injury group,erectile function was significantly recovered in the ADSCGFP and ADSCrBDNF groups,and to a greater degree in the ADSCrBDNF *** nitric oxide synthase content in the dorsal nerves and the ratio of smooth muscle/collagen were significantly higher in the ADSCrBDNF and ADSCGFP groups than in the bilateral cavernous nerve injury *** nitric oxide synthase expression was obviously higher in the ADSCrBDNF group than in the ADSCGFP *** findings confirm that intracavernous injection with ADSCs infected with lenti-rBDNF can effectively improve erectile dysfunction caused by cavernous nerve *** study was approved by the Medical Animal Care and Welfare Committee of

关键词： adipose-derived stem cells brain-derived neurotrophic factor cavernous nerve injury erectile dysfunction infection intracavernous injection lentiviral vector neuronal nitric oxide synthase radical prostatectomy

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G protein-coupled receptor kinase 2 inhibition improves erectile function through amelioration of endothelial dysfunction and oxidative stress in a rat model of type 2 diabetes

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Asian Journal of Andrology 2019年第1期21卷 74-79页

作者： Zhi-Hua Wan Yuan-Jie Zhang Lin Chen yong-lian guo guo-Hao Li Ding Wu yong Wang Department of Urology The Central Hospital of WuhanTongll Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China

Type 2 diabetes mellitus (T2DM)is a common cause of erectile dysfunction (ED).It has been demonstrated that G protein-coupled receptor kinase 2 (GRK2)overexpression contributes to diabetic endothelial dysfunction and oxidative stress,which also underlies ED in *** hypothesized that GRK2 overexpressed and attenuated endothelial function of the cavernosal tissue in a rat model of T2DM.T2DM rats were established by feeding with a high-fat diet (HFD)for 2 weeks and then administering two intraperitoneal (IP) injections of a low dose of streptozotocin (STZ),followed by continuous feeding with a HFD for 6 ***2 was inhibited by IP injection of paroxetine,a selective GRK2 inhibitor,after STZ *** challenge tests,intracavernous pressure (ICP), GRK2 expression,the protein kinase B (Akt)/endothelial nitric oxide synthase (eNOS)pathway,nicotinamide adenine dinucleotide phosphate (NADPH)oxidase subunit gp91phox,nitric oxide (NO),reactive oxygen species (ROS)production,and apoptosis in cavernosal tissue were *** response to insulin injection was observed in T2DM rats 2 weeks after *** increased GRK2 expression,along with impaired Akt/eNOS pathway,reduced NO production,increased gp91phox expression and ROS generation,increased apoptosis and impaired erectile function were found in T2DM *** of GRK2 with paroxetine ameliorated Akt/eNOS signaling,restored NO production,downregulated NADPH oxidase,subsequently inhibited ROS generation and apoptosis,and ultimately preserved erectile *** results indicated that GRK2 upregulation may be an important mechanism underlying T2DM ED,and GRK2 inhibition may be a potential therapeutic strategy for T2DM ED.

关键词： endothelial dysfunction erectile dysfunction G protein-coupled receptor kinase 2 oxidative stress type 2 diabetes mellitus

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Neural-like cells from adipose-derived stem cells for cavernous nerve injury in rats

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Neural Regeneration Research 2019年第6期14卷 1085-1090页

作者： Cheng-Cheng Ying Mei Yang yong Wang yong-lian guo Wan-Li Hu Xin-Min Zheng Department of Urology the Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and Technology Department of Endocrinology Tongren Hospital of Wuhan University (Wuhan Third Hospital) Department of Urology Zhongnan Hospital of Wuhan University

Although the remaining nerve tissue can regenerate and partly restore erectile function when the cavernous nerve is compressed/severed and function lost, the limited regenerative ability of these nerve tissues often fails to meet clinical needs. Adipose-derived stem cells are easy to obtain and culture, and can differentiate into neural cells. Their proliferation rate is easy to control and they may be used to help restore injured cavernous nerve function. Sprague-Dawley male rats(n = 45) were equally randomized into three groups: fifteen rats as a sham-operated group, fifteen rats as a bilateral nerve crush(BINC) group(with no further intervention), fifteen rats as a BINC with intracavernous injection of one million neural-like cells from adipose-derived stem cells(NAS)(BINC + NAS) group. After 4 weeks, erectile function was assessed by stimulating the cavernous body. The number of myelinated axons in the dorsal cavernous nerve was determined by toluidine blue staining. The area of neuronal nitric oxide synthase-positive fibers in the dorsal penile nerve was measured by immunohistochemical staining. Masson staining was used to analyze the ratio of smooth muscle to collagen in penile tissue. The results demonstrate that maximal intracavernous pressure, the ratio of maximal intracavernous pressure to mean arterial pressure, the numbers of myelinated axons and neuronal nitric oxide synthase-positive fibers in the dorsal penile nerve, and the ratio of smooth muscle to collagen could be increased after cell transplantation. These findings indicate that neural-like cells from adipose-derived stem cells can effectively alleviate cavernous nerve injury and improve erectile function. All animal experiments were approved by the Animal Ethics Committee of Huazhong University of Science and Technology, China(approval No. 2017-1925) on September 15, 2017.

关键词： nerve regeneration adipose-derived neural stem cells corpus cavernosum cavernous nerve erectile dysfunction radical prostatectomy neurons cell differentiation neural regeneration

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玉米螟赤眼蜂(Trichogramma ostriniae)寄主标记信息素和学习经历对产卵行为的影响

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生态学报 2007年第9期27卷 3910-3915页

作者：王素琴练永国康总江墨铁路北京市农林科学院植物保护环境保护研究所北京100097 山东农业大学植物保护学院昆虫学系泰安271000

报道了标记信息素和雌蜂经历对玉米螟赤眼蜂Trichogramma ostriniae产卵行为的影响。研究结果表明,玉米螟赤眼蜂的寄主标记信息素是在其产卵过程中的收场阶段由产卵器产生的,并且标记在寄主卵表的化学物质;玉米螟赤眼蜂对标记信息素的... 详细信息

报道了标记信息素和雌蜂经历对玉米螟赤眼蜂Trichogramma ostriniae产卵行为的影响。研究结果表明,玉米螟赤眼蜂的寄主标记信息素是在其产卵过程中的收场阶段由产卵器产生的,并且标记在寄主卵表的化学物质;玉米螟赤眼蜂对标记信息素的识别不具有先天性,其必须先用产卵器插入寄生卵来检测是否被寄生,然后其触角不停地敲击,通过学习获得对标记信息素的识别能力,从而放弃了对寄生卵的选择,学习对初始放弃时间有着重要影响,改善了其对寄主搜索效率,这种学习行为是与寄主联系在一起学习的,它能根据寄主被寄生与否而对标记信息素作出反应与不反应。该研究丰富了生态学理论,为其在生防中更好的应用提供了科学依据。

关键词：寄主标记信息素经历玉米螟赤眼蜂产卵行为初始放弃时间

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ImSpiRE: image feature-aided spatial resolution enhancement method

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Science China Life Sciences 2025年第1期 272-283页

作者： Yuwei Hua Yizhi Zhang Zhenming guo Shan Bian yong Zhang State Key Laboratory of Cardiovascular Diseases and Medical Innovation Center Institute for Regenerative MedicineDepartment of NeurosurgeryShanghai East HospitalShanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji University

The resolution of most spatially resolved transcriptomic technologies usually cannot attain the single-cell level, limiting their applications in biological discoveries. Here, we introduce ImSpiRE, an image feature-aided spatial resolution enhancement method for in situ capturing spatial transcriptome. Taking the information stored in histological images, ImSpiRE solves an optimal transport problem to redistribute the expression profiles of spots to construct new transcriptional profiles with enhanced resolution, together with extending the gene expression profiles into unmeasured regions. Applications to multiple datasets confirm that ImSpiRE can enhance spatial resolution to the subspot level while contributing to the discovery of tissue domains, signaling communication patterns, and spatiotemporal characterization.

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Viscosity and structure relationship with equimolar substitution of CaO with MgO in the CaO–MgO–Al_(2)O_(3)–SiO_(2)slag melts

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 70-79页

作者： yong Hou Shuo Zhang Jie Dang Jia guo Hanghang Zhou Xuewei Lü College of Materials Science and Engineering Chongqing UniversityChongqing 400044China Chongqing Key Laboratory of Vanadium-Titanium Metallurgy and New Materials Chongqing UniversityChongqing 400044China

Currently,the Al_(2)O_(3)content in the high-alumina slag systems within blast furnaces is generally limited to 16wt%–18.5wt%,making it challenging to overcome this *** most studies that concentrated on managing the MgO/Al_(2)O_(3)ratio or basicity,this paper explored the effect of equimolar substitution of MgO for CaO on the viscosity and structure of a high-alumina CaO-MgO-Al_(2)O_(3)-SiO_(2)slag system,providing theoretical guidance and data to facilitate the application of high-alumina *** results revealed that the viscosity first decreased and then increased with higher MgO substitution,reaching a minimum at 15mol%MgO *** transform infrared spectroscopy(FTIR)results found that the depths of the troughs representing[SiO_(4)]tetrahedra,[AlO_(4)]tetrahedra,and Si-O-Al bending became progressively deeper with increased MgO *** of the Raman spectra showed that the average number of bridging oxygens per Si atom and the X_(Q^(3))/X_(Q^(2))(X_(Q^(i))is the molar fraction of Q^(i) unit,and i is the number of bridging oxygens in a[SiO_(4)]tetrahedral unit)ratio increased from 2.30 and 1.02 to 2.52 and 2.14,respectively,indicating a progressive polymerization of the silicate structure.X-ray photoelectron spectroscopy(XPS)results highlighted that non-bridging oxygen content decreased from 77.97mol% to 63.41mol% with increasing MgO concentration,whereas bridging oxygen and free oxygen contents *** analysis demonstrated a gradual increase in the polymerization degree of the tetrahedral structure with the increase in MgO ***,bond strength is another important factor affecting the slag *** occurrence of a viscosity minimum can be attributed to the complex evolution of bond strengths of non-bridging oxygens generated during depolymerization of the[SiO_(4)]and[AlO_(4)]tetrahedral structures by CaO and MgO.

关键词： aluminosilicate viscosity structure spectroscopy

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Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

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Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu guo Lanyue Zhang Gening Du Hao You Junfeng Huang Mudong Tu Hua Li yong Peng Wei Dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 mA cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： NiFe-MIL-53 Rh Electrochemical reconstruction Catalytic mechanism Oxygen evolution reaction

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卵液成分改变及卵表涂施引诱剂对玉米螟赤眼蜂产卵发育的影响

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昆虫知识 2009年第4期46卷 551-556页

作者：练永国王素琴白树雄康总江王振营山东农业大学植物保护学院昆虫学系泰安271000 北京市农林科学院植物保护环境保护研究所北京100097 中国农业科学院植物保护研究所北京100094

试验通过部分改变人造卵卵液成分和在人造卵表涂施引诱剂2种途径来试图提高人造卵繁育玉米螟赤眼蜂Trichogramma ostriniae Panget Chen的成功率。结果表明,不同比例的亚洲玉米螟OstriniafurnacalisGuenée幼虫和蛹匀浆替代部分柞蚕蛹... 详细信息

试验通过部分改变人造卵卵液成分和在人造卵表涂施引诱剂2种途径来试图提高人造卵繁育玉米螟赤眼蜂Trichogramma ostriniae Panget Chen的成功率。结果表明,不同比例的亚洲玉米螟OstriniafurnacalisGuenée幼虫和蛹匀浆替代部分柞蚕蛹匀浆后,对赤眼蜂寄生率有明显提高,但赤眼蜂在人造卵内的化蛹率和羽化率均较低,赤眼蜂发育至老熟幼虫因卵液过剩而被淹死;在原卵液卵表涂施亚洲玉米螟卵和鳞片正己烷提取液后,评价繁蜂成功率的各项参数都有明显提高,赤眼蜂可以完成发育,羽化成蜂,但繁蜂成功率还较低。综合上述试验结果,应用人造卵繁殖玉米螟赤眼蜂研究重点要解决卵液过剩以及其它相关的营养学问题。

关键词：亚洲玉米螟玉米螟赤眼蜂人造卵引诱剂

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PCR检测实验恒河猴和食蟹猴群体中幽门螺杆菌和“猕猴螺杆菌”的感染

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中国比较医学杂志 2016年第10期26卷 61-68页

作者：王立鹏李永旺郭连香时长军苏州西山生物技术有限公司江苏215123

目的调查国内实验恒河猴和食蟹猴中幽门螺杆菌和"猕猴螺杆菌"的感染情况。方法参考文献中的螺杆菌属16S rRNA和幽门螺杆菌16S rRNA的引物序列,和新设计的"猕猴螺杆菌"16S rRNA特异性引物,在人工养殖的45只成年恒河猴和90只成年食蟹猴粪... 详细信息

目的调查国内实验恒河猴和食蟹猴中幽门螺杆菌和"猕猴螺杆菌"的感染情况。方法参考文献中的螺杆菌属16S rRNA和幽门螺杆菌16S rRNA的引物序列,和新设计的"猕猴螺杆菌"16S rRNA特异性引物,在人工养殖的45只成年恒河猴和90只成年食蟹猴粪便样本中,通过q PCR或常规PCR检测来初步调查这两种猕猴中两种螺杆菌的感染情况。结果在恒河猴中幽门螺杆菌和"猕猴螺杆菌"的感染率均为100%,在食蟹猴中幽门螺杆菌和"猕猴螺杆菌"的感染率分别为100%和97.8%。结论证实我国人工繁育饲养的恒河猴和食蟹猴普遍存在"猕猴螺杆菌"感染。幽门螺杆菌和"猕猴螺杆菌"几乎同时存在于所有人工繁育的实验猴个体中,可能会对这两种猕猴的健康以及相关动物实验结果的准确性存在不利影响。

关键词：恒河猴食蟹猴幽门螺杆菌猕猴螺杆菌感染诊断

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挥发性信息化合物对赤眼蜂寄生行为的影响及其利用

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中国生物防治 2007年第1期23卷 89-92页

作者：练永国王素琴王振营墨铁路康总江山东农业大学植物保护学院昆虫学系泰安271000 北京市农林科学院植物保护与环境保护研究所北京100097 中国农科院植保所北京100094

本文简述了挥发性物质对赤眼蜂寄生行为的调控作用以及其在生防中的应用,并探讨了挥发性信息化合物对赤眼蜂行为调控的研究意义与应用前景。

关键词：挥发性信息化合物赤眼蜂人造卵寄生行为

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