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Effect of aging temperature on the microstructures and mechanical properties of ZG12Cr9Mo1Co1NiVNbNB ferritic heat-resistant steel

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International Journal of Minerals,Metallurgy and Materials 2016年第2期23卷 168-175页

作者： Xue Yang Lan Sun Ji Xiong Ping Zhou Hong-yuan Fan jian-yong liu School of Manufacturing Science and Engineering Sichuan UniversityChengdu 610065China Dong Fang Steam Turbine Works Deyang 618000China

The effect of aging on the mechanical properties and microstructures of a new ZG12Cr9 MolColNiVNbNB ferritic heat resistant steel was investigated in this work to satisfy the high steam parameters of the ultra-supercritical power *** results show that the main precipitates during aging are Fe（Cr,Mo）23C6,V（Nb）C,and（Fe2Mo） Laves in the *** amounts of the precipitated phases increase during aging,and correspondingly,the morphologies of phases are similar to be ***（Cr,Mo）23C6 appears along boundaries and grows with increasing *** addition,it is revealed that the martensitic laths are coarsened and eventually happen to be *** hardness and strength decrease gradually,whereas the plasticity of the steel ***＇s more,the hardness of this steel after creep is similar to that of other 9%-12%Cr ferritic ***,ZG12Cr9 MolColNiVNbNB can be used in the project.

关键词： heat resistant steel aging temperature precipitates microstructure mechanical properties

Oncological impact of different distal ureter managements during radical nephroureterectomy for primary upper urinary tract urothelial carcinoma

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World Journal of Clinical Cases 2020年第21期8卷 5104-5115页

作者： Shi-Cong Lai Peng-Jie Wu jian-yong liu Samuel Seery Sheng-Jie liu Xing-Bo Long Ming liu jian-Ye Wang Department of Urology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100730China School of Humanities and Social Sciences Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

BACKGROUND The current standard surgical treatment for non-metastatic upper urinary tract urothelial carcinoma(UTUC)is radical nephroureterectomy(RNU)with bladder cuff excision(BCE).Typically,BCE techniques are classified in one of the following three categories:An open technique described as intrasvesical incision of the bladder cuff,a transurethral incision of the bladder cuff(TUBC),and an extravesical incision of the bladder cuff(EVBC)*** though each of these management techniques are widely used,there is no consensus about which surgical intervention is superior,with the best oncologic *** To investigate the oncological outcomes of three BCE methods during RNU for primary UTUC *** We retrospectively analyzed the data of 248 primary UTUC patients,who underwent RNU with BCE between January 2004 to December *** were analyzed according to each BCE *** extracted included patient demographics,perioperative parameters,and oncological *** analyses were performed using chi-square and log-rank *** Cox proportional hazards regression model was utilized to identify independent predictors.P<0.05 was considered statistically *** Of the 248 participants,39.9%(n=99)underwent intrasvesical incision of the bladder cuff,38.7%(n=96)EVBC,and 21.4%(n=53)*** a median followup of 44.2 mo,bladder recurrence developed in 17.2%,12.5%,and 13.2%of the cases,***-specific deaths occurred in 11.1%,5.2%,and 7.5%of patients,***-Meier survival curves with a log-rank test highlighted no significant differences in intravesical recurrence-free survival,cancer-specific survival,and overall survival among these approaches with P values of 0.987,0.825,and 0.497,*** analysis showed that the lower ureter location appears to have inferior intravesical recurrence-free survival(P=0.042).However,cancer-specific survival and overall survival were independently influe

关键词： Bladder cuff excision Prognosis Upper urinary tract urothelial carcinoma Radical nephroureterectomy Technique Survival

Cognitive magnetic resonance imaging-ultrasound fusion transperineal targeted biopsy combined with randomized biopsy in detection of prostate cancer

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World Journal of Clinical Cases 2021年第36期9卷 11183-11192页

作者： Cheng Pang Miao Wang Hui-Min Hou jian-yong liu Zhi-Peng Zhang Xuan Wang Ya-Qun Zhang Chun-Mei Li Wei Zhang jian-Ye Wang Ming liu Department of Urology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Graduate School of Peking Union Medical College Chinese Academy of Medical SciencesBeijing 100730China Department of Radiology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730BeijingChina Department of Pathology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730BeijingChina

BACKGROUND Prostate cancer(PCa)is one of the most common cancers among *** strategies for targeted biopsy based on multiparametric magnetic resonance imaging(mp-MRI)have emerged,which may improve the accuracy of detecting clinically significant PCa in recent *** To investigate the diagnostic efficiency of a template for cognitive MRIultrasound fusion transperineal targeted plus randomized biopsy in detecting *** Data from patients with an increasing prostate-specific antigen(PSA)level but less than 20 ng/mL and at least one lesion suspicious for PCa on MRI from December 2015 to June 2018 were retrospectively *** patients underwent cognitive fusion transperineal template-guided targeted biopsy followed by randomized biopsy outside the targeted area.A total of 127 patients with complete data were included in the final analysis.A multivariable logistic regression analysis was conducted,and a two-sided P<0.05 was considered statistically *** PCa was detected in 66 of 127 patients,and 56 cases presented clinically significant *** fusion targeted biopsy alone detected 59/127 cases of PCa,specifically 52/59 cases with clinically significant PCa and 7/59 cases with clinically insignificant PCa.A randomized biopsy detected seven cases of PCa negative on targeted biopsy,and four cases had clinically significant *** density(OR:1.008,95%CI:1.003-1.012,P=0.001;OR:1.006,95%CI:1.002-1.010,P=0.004)and Prostate Imaging-Reporting and Data System(PI-RADS)scores(both P<0.001)were independently associated with the results of cognitive fusion targeted biopsy combined with randomized biopsy and targeted biopsy *** This single-centered study proposed a feasible template for cognitive MRIultrasound fusion transperineal targeted plus randomized *** with higher PSAD and PI-RADS scores were more likely to be diagnosed with PCa.

关键词： Prostate neoplasms Magnetic resonance imaging Cognitive fusion Prostate biopsy Prostate cancer

Excess dietary cholesterol may have an adverse effect on growth performance of early post-larval Litopenaeus vannamei

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Journal of Animal Science and Biotechnology 2012年第3期3卷 155-159页

作者： Jin Niu Peng-Fei Chen Li-Xia Tian yong-jian liu Hei-Zhao Lin Hui-Jun Yang Gui-Ying Liang Key Laboratory of Aquatic Product Processing.Ministry of Ag riculture South China Sea Fisheries Research Institute Chinese Academy of Fishery ScienceGuangzhou 510300People's Republic of China Nutrition Laboratory Institute of Aquatic Economic AnimalsSchool of Life ScienceSun Yat-sen UniversityGuangzhou 510275People's Republic of China

One experiment was conducted to determine the nutritive value of cholesterol for post-larval shrimp, Litopenaeus vannameL Four isoenergetic and isonitrogenous diets supplemented with four levels of cholesterol （D1, D2, D3 and D4 with 0, 0.5%, 1% and 2% cholesterol, respectively） were fed to triplicate groups of L. vannamei shrimp （mean initial wet weight 0.8 mg） for 27 days. After the trial, shrimp fed the D1 diet had the best growth performance （final body weights： FBW; weight gain： WG; specific growth rate： SGR）, while there was no significant difference between diet treatments with respect to survival. The whole body crude protein level in the shrimp decreased with the increase in dietary cholesterol levels, while the whole body crude lipid level in shrimps in the D4 diet treatment was significantly higher （P 〈 0.05） than in other diet treatments. Dietary analysis indicated that the D1 diet contained 0.92% cholesterol prior to supplementation, which may have satisfied the dietary cholesterol requirement of post-larval L. vannamei; excess dietary cholesterol may thus lead to adverse effects on the growth performance of post-larval shrimp.

关键词： Cholesterol Growth Larvae Lipid classes Litopenaeus vannamei Survival

Quantitative differentiation of dendritic cells in lung tissues of smokers with and without chronic obstructive pulmonary disease

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Chinese Medical Journal 2010年第12期123卷 1500-1504页

作者： SU Yan-wei XU yong-jian liu Xian-sheng Department of Respiratory Medicine Tongji Hospital affiliated to Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Chronic obstructive pulmonary disease （COPD） is thought to be an inflammatory immune response disease. In most cases, the disease is caused by cigarette smoke, but it has been demonstrated that only 10% to 20% of smokers will definitely suffer from COPD. Dendritic cells （DCs） are considered to be the promoter of immune ***, the underlying mechanisms involved are still unrevealed. In this study, we aimed to investigate the quantitative differentiation of pulmonary DC in smokers with or without COPD to explore the possible role of DCs in smokers suffering *** Peripheral lung specimens from non-smokers without airflow obstruction （control group, n=7）, smokers without airflow obstruction （smoker group, n=7） and patients with COPD （COPD group, n=7） were investigated to detect the quantity of S-100 and CD1a positive cells by immunohistochemical or immunofluorescent *** In smokers with COPD, the number of S-100＋ DCs was higher than in the controls and smokers without COPD （P 〈0.01 and P 〈0.05） and there was a higher number of S-100＋ DCs in smokers with COPD than in smokers without COPD, but without a significant difference （P 〉0.05）. An inverse correlation was found between the number of DCs and forced expiratory volume in the first second （FEV1）% pred （r=-0.75, P 〈0.05）, which was also found between the number of DCs and FEV1/forced vital capacity （FVC）（r=-0.72, P 〈0.05）. The mean number of CD1a＋ DCs, increased from non-smokers to non-COPD smokers to COPD patients, with significant differences between each group （P 〈0.01）.Conclusions The quantity of DCs significantly increased in smokers with COPD compared with non-smokers or smokers without COPD. The results suggest that DCs may play an important role in the pathogenesis of smoking-induced COPD, and the upregulation of DCs may be a potential maker to identify the smokers who have more liability to suffer from COPD.

关键词： dendritic cells smoking chronic obstructive pulmonary disease

Indinavir Resistance Evolution in One Human Immunodeficiency Virus Type 1 Infected Patient Revealed by Single-Genome Amplification

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Virologica Sinica 2010年第5期25卷 316-328页

作者： Qing-mao GENG Han-ping LI Zuo-yi BAO yong-jian liu Dao-min ZHUANG Lin LI Si-yang liu Jing-yun LI State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing100071 China

Human Immunodeficiency Virus Type 1 exists in vivo as quasispecies, and one of the genome's characteristics is its diversity. During the antiretroviral therapy, drug resistance is the main obstacle to effective viral prevention. Understanding the molecular evolution process is fundamental to analyze the mechanism of drug resistance and develop a strategy to minimize resistance. Objective: The molecular evolution of drug resistance of one patient who had received reverse transcriptase inhibitors for a long time and had treatment which replaced Nevirapine with Indinavir was analyzed, with the aim of observing the drug resistance evolution pathway. Methods: The patient, XLF, was followed-up for six successive times. The viral populations were amplified and sequenced by single-genome amplification. All the sequences were submitted to the Stanford HIV Drug Resistance Database for the analysis of genotypic drug resistance. Results: 149 entire protease and 171 entire reverse transcriptase sequences were obtained from these samples, and all sequences were identified as subtype B. Before the patient received Indinavir, the viral population only had some polymorphisms in the protease sequences. After the patient began Indinavir treatment, the variants carrying polymorphisms declined while variants carrying the secondary mutation G73S gained the advantage. As therapy was prolonged, G73S was combined with M46I/L90M to form a resistance pattern M46I/G73S/L90M, which then became the dominant population. 97.9% of variants had the M46I/G73S/L90M pattern at XLF6. During the emergence of protease inhibitors resistance, reverse transcriptase inhibitors resistance maintained high levels. Conclusion: Indinavirresistance evolution was observed by single-genome amplification. During the course of changing the regimen to incorporate Indinavir, the G73S mutation occurred and was combined with M46I/L90M.

关键词： Single-Genome Amplification Indinavir Resistance Evolution M46I/G73S/L90M Mutation Pattern

Cigarette smoke extract promotes human pulmonary artery smooth muscle cells proliferation through protein kinase C alpha-dependent induction of cyclin D1

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Chinese Medical Journal 2010年第24期123卷 3663-3670页

作者： XIANG Min XU yong-jian liu Xian-sheng ZENG Da-xiong Department of Respiratory Medicine Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 Chin Department of Respiratory Medicine Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Exposure to cigarette smoke stimulates the proliferation of human pulmonary artery smooth muscle cells （HPASMCs） in vivo and in vitro. However, the molecular mechanism remains unclear. This study aimed at investigating the role of signaling pathways involving protein kinase C alpha （PKCα） and cyclin D1 in the cigarette smoke extract （CSE）-induced HPASMCs *** Synchronized HPASMCs were treated with different concentrations of CSE. Cell proliferation was evaluated by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyttetrazolium bromide （MTT） assay and cell counting. Cell cycle was analyzed by flow cytometry with propidium iodide staining. Activation of PKCα was measured by detecting the expression of PKCαprotein in the cytosolic and membrane fractions using Western blotting analysis. Small interfering RNA （siRNA） was used to knockdown PKCα and cyclin D1. The cyclin D1 mRNA was assessed by real-time RT-PCR. The PKCα and cyclin D1protein levels were detected by Western *** Low concentrations of CSE （1%-10%） stimulated proliferation of HPASMCs, with its maximal effect at 5%.CSE （5%） led to PKCα activation. Inhibition of PKCα activity using G（o） 6976 or siRNA-mediated knockdown of PKCα significantly attenuated CSE-induced cell proliferation and G1/S transition. Cyclin D1, one of key regulators of G1/S transition, was found to be upregulated by 5% CSE at both the mRNA and protein levels. CSE-stimulated cellproliferation and G1/S transition was abolished by cyclin D1 siRNA. Moreover, G（o） 6976 or PKCα siRNA significantlysuppressed CSE-induced upregulation of cyclin D1 at both the mRNA and protein *** PKCα-cyclin D1 pathway at least partially mediates the CSE-induced proliferation in HPASMCs.

关键词： cell proliferation cell cycle protein kinase C cyclin D1

HIV-1 Vif Protein Mediates the Degradation of APOBEC3G in Fission Yeast When Over-expressed Using Codon Optimization

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Virologica Sinica 2008年第4期23卷 255-264页

作者： Lin LI Jing-yun LI Hong-shuai SUI Richard Y. Zhao yong-jian liu Zuo-yi BAO Si-yang liu Dao-min ZHUANG AIDS Research Department State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Being 100071 China Department of Pathology Department of Microbiology- Immunology Institute of Human Virology University of Maryland 10 South Pine Street MSTF7OOA Baltimore MD 21201 USA

Interaction between the HIV-1 Vif protein and the cellular host APOBEC3G protein is a promising target for inhibition of HIV-1 replication. Considering that human cells are a very complicated environment for the study of protein interactions, the goal of this study was to check whether fission yeast could be used as a model cell for studying the Vif-APOBEC3G interaction. Vif and APOBEC3G were expressed in fusion with GFP protein in the S. pombe SP223 strain. Subcellular localizations of Vif and APOBEC3G were observed with fluorescent microscopy. Codon optimization was used to over express the Vif protein in S. pombe cells. The degradation of APOBEC3G mediated by Vif was tested through expressing Vif and GFP-APOBEC3G proteins in the same cell. Western Blot analysis was used to measure the corresponding protein levels under different experimental conditions. The results showed that the Vif protein was predominantly localized in the nucleus of S. pombe cells, APOBEC3G was localized in the cytoplasm and concentrated at punctate bodies that were often in close proximity to the nucleus but were not necessarily restricted from other regions in the cytoplasm. Vif protein expression levels were increased significantly by using codon optimization and APOBEC3G was degraded when Vif was over-expressed in the same S. pombe cells. These results indicate that fission yeast is a good model for studying the interaction between the Vif and APOBEC3G proteins.

关键词： HIV Vif protein APOBEC3G Fission yeast (Schizosaccharomyces pombe)

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Virologica Sinica 2012年第3期27卷 165-171页

作者： Zheng Wang Tian-yi Li Jing-yun Li Li-li Chen yong-jian liu Han-ping Li Zuo-yi Bao Xiao-lin Wang Dao-min Zhuang Si-yang liu Lin Li Department of AIDS Research State Key Laboratory of Pathogen and BiosecurityBeo'ing Institute of Microbiology and EpidemiologyBeijing 100071China Urumqi General Hospital of Lanzhou Military Area Command Urumqi 830000China

Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the neutralizing antibody response in the plasma from LTNP and TP against clade B and clade C pseudoviruses. No difference in the neutralizing activities between the plasma from LTNP and TP was found, which was consistent with the most recent reports. In addition, no correlations between the titer or breadth and CD4+ or viral load in HIV-1 infected individuals were found. The protective roles played by neutralizing antibodies in controlling disease progression of HIV-1 infected people need to be considered in a new viewpoint.

关键词： HIV-1 Long term non-progressor (LTNP) Typical progressor (TP) Neutralization activity