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3D printed silk-gelatin hydrogel scaffold with different porous structure and cell seeding strategy for cartilage regeneration

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Bioactive Materials 2021年第10期6卷 3396-3410页

作者： Qingtao Li Sheng xu Qi Feng Qiyuan Dai Longtao Yao Yichen Zhang Huichang Gao hua Dong Dafu Chen Xiaodong Cao National Engineering Research Center for Tissue Restoration and Reconstruction(NERC-TRR) South China University of TechnologyGuangzhouGuangDong510641China Department of Biomedical Engineering School of Material Science and EngineeringSouth China University of TechnologyGuangzhouGuangDong510641China School of Medicine South China University of TechnologyGuangzhouGuangDong510641China Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of EducationInnovation Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhouGuangDong510641China Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Orthopaedics and TraumatologyBeijing JiShuiTan HospitalBeijing100035China Zhongshan Institute of Modern Industrial Technology of SCUT ZhongshanGuangdong528437China

Hydrogel scaffolds are attractive for tissue defect repair and reorganization because of their human tissue-like ***,most hydrogels offer limited cell growth and tissue formation ability due to their submicron-or nano-sized gel networks,which restrict the supply of oxygen,nutrients and inhibit the proliferation and differentiation of encapsulated *** recent years,3D printed hydrogels have shown great potential to overcome this problem by introducing macro-pores within *** this study,we fabricated a macroporous hydrogel scaffold through horseradish peroxidase(HRP)-mediated crosslinking of silk fibroin(SF)and tyramine-substituted gelatin(GT)by extrusion-based low-temperature 3D *** physicochemical characterization,we found that this hydrogel has excellent structural stability,suitable mechanical properties,and an adjustable degradation rate,thus satisfying the requirements for cartilage *** suspension and aggregate seeding methods were developed to assess the inoculation efficiency of the ***,the chondrogenic differentiation of stem cells was *** cells in the hydrogel differentiated into hyaline cartilage when the cell aggregate seeding method was used and into fibrocartilage when the cell suspension was ***,the effect of the hydrogel and stem cells were investigated in a rabbit cartilage defect *** implantation for 12 and 16 weeks,histological evaluation of the sections was *** found that the enzymatic cross-linked and methanol treatment SF5GT15 hydrogel combined with cell aggregates promoted articular cartilage *** summary,this 3D printed macroporous SF-GT hydrogel combined with stem cell aggregates possesses excellent potential for application in cartilage tissue repair and regeneration.

关键词： Silk hydrogel 3D printing Enzymatic cross-linking Cell aggregate seeding Cartilage regeneration

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Identification of differential gene expression profiles of radioresistant lung cancer cell line established by fractionated ionizing radiation in vitro

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Chinese Medical Journal 2008年第18期121卷 1830-1837页

作者： xu Qing-yong GAO Yuan LIU Yan YANG Wei-zhi xu Xiang-ying Department of Radiation Oncology Cancer Hospital Harbin Medical University Harbin Heilongjiang 150081 China Department of Biochemistry Harbin Medical University Harbin Heilongjiang 150086 China Department of Radiobiology Cancer Institute (Hospital) Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100021 China

Background Radiotherapy plays a critical role in the management of non-small cell lung cancer （NSCLC）. This study was conducted to identify gene expression profiles of acquired radioresistant NSCLC cell line established by fractionated ionizing radiation （FIR） by cDNA microarray. Methods The human lung adenocarcinoma cell line Anip973 was treated with high energy X-ray to receive 60 Gy in 4 Gy fractions. The radiosensitivity of Anip973R and its parental line were measured by clonogenic assay. Gene expression profiles of Anip973R and its parental line were analyzed using cDNA microarray consisting of 21 522 human genes. Identified partly different expressive genes were validated by quantitative reverse transcription-polymerase chain reaction （Q-RT-PCR）. Results Fifty-nine upregulated and 43 downregulated genes were identified to radio-resistant Anip973R. Up-regulated genes were associated with DNA damage repair （DDB2）, extracellular matrix （LOX）, cell adhesion （CDH2）, and apoptosis （CRYAB）. Down-regulated genes were associated with angiogenesis （GBP-1）, immune response （CD83）, and calcium signaling pathway （TNNC1）. Subsequent validation of selected eleven genes （CD24, DDB2, IGFBP3, LOX, CDH2, CRYAB, PROCR, ANXA1 DCN, GBP-1 and CD83） by Q-RT-PCR was consistent with microarray analysis. Conclusions Fractionated ionizing radiation can lead to the development of radiation resistance. Altered gene profiles of radioresistant cell line may provide new insights into mechanisms underlying clinical radioresistance for NSCLC.

关键词： radioresistance cDNA microarray non-small cell lung cancer fractionated ionizing radiation

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生活垃圾焚烧残灰中有毒成分的排放特性（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2015年第4期16卷 316-325页

作者： Xiao-dong LI yong REN Sha-sha JI Xia-li HOU Tong CHEN Sheng-yong LU Jian-hua YAN State Key Laboratory of Clean Energy Utilization Zhejiang University

目的:探索中国生活垃圾焚烧残灰中重金属、氯苯、多环芳烃等有毒成分的排放特性、排放水平及相互之间的关联特性,并认识其产生、排放与焚烧炉型、焚烧条件的关联,以探索控制其危害的有效方法。方法:1.对中国几个典型的生活垃圾焚烧炉现... 详细信息

目的:探索中国生活垃圾焚烧残灰中重金属、氯苯、多环芳烃等有毒成分的排放特性、排放水平及相互之间的关联特性,并认识其产生、排放与焚烧炉型、焚烧条件的关联,以探索控制其危害的有效方法。方法:1.对中国几个典型的生活垃圾焚烧炉现场采样,获得多个飞灰和底渣的样品;2.通过多种不同的检测手段和方法,分别检测残灰的基本物理化学特性、氯苯、多环芳烃和主要金属元素的浓度;3.结合焚烧炉型和焚烧特性等条件,分析各有毒成分的排放特性和相互之间的关联特性。结论:1.氯苯、多环芳烃和重金属受焚烧因素影响,在残灰中的排放特性各不相同,流化床焚烧炉能消除焚烧和原始垃圾的扰动,能控制氯苯在残灰中的排放,但多环芳烃排放控制不如炉排焚烧炉;2.残灰中主要的有机有毒成分为高氯代氯苯和2至4环等少环类多环芳烃;3.氯苯和多环芳烃在残灰中的含量可能因为不同的产生机理而表现出一定的负关联特点;4.残灰中的金属主要为铝和铁等轻金属,浓度远高于重金属元素,而无毒重金属(主要为Mn、Ni、As和Zn)浓度高于有毒重金属元素(Cu、Pb和Cr),且不同金属表现出不同的对氯苯和多环芳烃的催化促进或抑制作用。

关键词：残灰氯苯多环芳烃重金属生活垃圾焚烧

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基于ARIS构建中烟工业公司业务流程管理系统

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中国烟草学报 2018年第5期24卷 77-83页

作者：华勇红塔烟草(集团)有限责任公司工艺质量部云南653100

为从企业全局的高度对业务流程进行系统性管理,以红塔集团为研究实例,构建中烟工业公司业务流程管理系统。系统不仅可以解决ERP业务流程管理"两张皮"的问题,提升ERP运维管理质量,而且通过设计中烟工业公司流程体系框架,创建公司流程体... 详细信息

为从企业全局的高度对业务流程进行系统性管理,以红塔集团为研究实例,构建中烟工业公司业务流程管理系统。系统不仅可以解决ERP业务流程管理"两张皮"的问题,提升ERP运维管理质量,而且通过设计中烟工业公司流程体系框架,创建公司流程体系模型,开发公司全局流程地图,实现了公司流程体系的全景式管理。通过管理思想与信息技术相结合,拓展流程管理应用,使该系统能够支撑并促进中烟工业公司流程体系管理的深入开展。

关键词：业务流程管理系统流程框架 ARIS SAP

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New Strategy for Prostate Cancer

New Strategy for Prostate Cancer

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第十届全国化学生物学学术会议

作者： yong xu Guangdong Provincial Key Laboratory of Biocomputing Guangzhou Institute of Biomedicine and HealthChinese Academy of Sciences

Prostate cancer（PCa） is the most common cancer in men and remains the second leading cause of cancer mortality in men.[1-3] Androgen-deprivation therapy（ADT） represents the gold standard treatment for PCa. However, disease will inevitably recur and progress to a lethal form named castration-resistant prostate cancer（CRPC）. CRPC remains androgen receptor（AR） signaling dependent and responds to recently approved androgen-signaling inhibitor enzalutamide and the androgen-synthesis inhibitor abiraterone acetate. Most patients eventually develop resistance to these second-generation therapies as well. Therefore, alternative strategy for development of next generation therapeutic agents is urgent needed. Here, we will present the identification and validation of new therapeutic targets for CRPC. By combining computational biology, computer-aided drug design with chemical biology and structural biology, some key nuclear receptors and related coregulator interaction network will be elucidated. As case studies, development of RORγ, BRD4 and CBP inhibitors against CRPC will be presented.

关键词： Prostate cancer CRPC Nuclear receptor Bromodomain

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Ligand Binding and Release Investigated by Contact-Guided Iterative Multiple Independent Molecular Dynamics Simulations

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Chinese Journal of Chemical Physics 2021年第3期34卷 334-342,I0044,I0045,I0048页

作者： Xin-fan hua Xin-zheng Du Zhi-yong Zhang National Science Center for Physical Sciences at the Microscale Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China

Binding and releasing ligands are critical for the biological functions of many proteins,so it is important to determine these highly dynamic *** there are experimental techniques to determine the structure of a protein-ligand complex,it only provides a static picture of the *** the rapid increase of computing power and improved algorithms,molecular dynamics(MD)simulations have diverse of superiority in probing the binding and release ***,it remains a great challenge to overcome the time and length scales when the system becomes *** work presents an enhanced sampling tool for ligand binding and release,which is based on iterative multiple independent MD simulations guided by contacts formed between the ligand and the *** the simulation results on adenylate kinase,we observe the process of ligand binding and release while the conventional MD simulations at the same time scale cannot.

关键词： Molecular dynamics simulation Enhanced sampling Binding and releasing process Adenylate kinase

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Deep learning model combined with computed tomography features to preoperatively predicting the risk stratification of gastrointestinal stromal tumors

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World Journal of Gastrointestinal Oncology 2024年第12期16卷 4663-4674页

作者： Yi Li Yan-Bei Liu xu-Bin Li Xiao-Nan Cui Dong-hua Meng Cong-Cong Yuan Zhao-Xiang Ye Department of Radiology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerTianjin’s Clinical Research Center for CancerState Key Laboratory of Druggability Evaluation and Systematic Translational MedicineTianjin Key Laboratory of Digestive CancerTianjin 300060China School of Life Sciences Tiangong UniversityTianjin 300387China Department of Radiology Tianjin First Central HospitalTianjin 300190China

BACKGROUND Gastrointestinal stromal tumors(GIST)are prevalent neoplasm originating from the gastrointestinal *** 50%of GIST patients experience tumor recurrence within 5 ***,there is a pressing need to accurately evaluate risk stratification *** To assess the application of a deep learning model(DLM)combined with computed tomography features for predicting risk stratification of *** Preoperative contrast-enhanced computed tomography(CECT)images of 551 GIST patients were retrospectively *** image features were independently analyzed by two *** parameters were statistically analyzed to identify significant predictors of high-risk *** were randomly assigned to the training(n=386)and validation cohorts(n=165).A DLM and a combined DLM were established for predicting the GIST risk stratification using convolutional neural network and subsequently evaluated in the validation *** Among the analyzed CECT image features,tumor size,ulceration,and enlarged feeding vessels were identified as significant risk predictors(P<0.05).In DLM,the overall area under the receiver operating characteristic curve(AUROC)was 0.88,with the accuracy(ACC)and AUROCs for each stratification being 87%and 0.96 for low-risk,79%and 0.74 for intermediate-risk,and 84%and 0.90 for high-risk,*** overall ACC and AUROC were 84%and 0.94 in the combined *** ACC and AUROCs for each risk stratification were 92%and 0.97 for low-risk,87%and 0.83 for intermediate-risk,and 90%and 0.96 for high-risk,*** in AUROCs for each risk stratification between the two models were significant(P<0.05).CONCLUSION A combined DLM with satisfactory performance for preoperatively predicting GIST stratifications was developed using routine computed tomography data,demonstrating superiority compared to DLM.

关键词： Gastrointestinal stromal tumors Deep learning Risk stratification Tomography,X-ray computed Prognosis

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CHRM3 is a novel prognostic factor of poor prognosis andpromotes glioblastoma progression via activation of oncogenicinvasive growth factors

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Oncology Research 2023年第6期31卷 917-927页

作者： BIN ZHANG JIANYI ZHAO yongZHI WANG hua xu BO GAO GUANGNING ZHANG BIN HAN GUOHONG SONG JUNCHEN ZHANG WEI MENG Department of Neurosurgery Affiliated Hospital of Jining Medical UniversityJiningChina Department of Neurosurgery The City Peoples’Hospital of FuyangFuyangChina Brain Injury Center Department of NeurosurgeryRen Ji HospitalShanghai Jiao Tong University School of MedicineShanghaiChina

Glioblastoma(GBM)is the most aggressive cancer of the brain and has a high mortality rate due to the lack of effective treatment *** of molecular mechanisms of GBM’s characteristic invasive growth is urgently needed to improve the poor ***-nuclear sequencing of primary and recurrent GBM samples revealed that levels of M3 muscarinic acetylcholine receptor(CHRM3)were significantly higher in the recurrent samples than in the primary ***,immunohistochemical staining of an array of GBM samples showed that high levels of CHRM3 correlated with poor prognosis,consistent with The Cancer Genome Atlas *** of CHRM3 inhibited GBM cell growth and *** assay of orthotopic GBM animal model in vivo indicated that inhibition of CHRM3 significantly suppressed GBM progression with prolonged survival *** analysis revealed that CHRM3 knockdown significantly reduced an array of classic factors involved in cancer invasive growth,including MMP1/MMP3/MMP10/MMP12 and CXCL1/CXCL5/*** together,CHRM3 is a novel and vital factor of GBM progression via regulation of multiple oncogenic genes and may serve as a new biomarker for prognosis and therapy of GBM patients.

关键词： Glioblastoma Prognosis CHRM3 MMP CXCL

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Comparison of tirofiban combined with dalteparin or unfractionated heparin in primary percutaneous coronary intervention of acute ST-segment elevation myocardial infarction patients

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Chinese Medical Journal 2011年第20期124卷 3275-3280页

作者： LI Wei-ming YANG Xin-chun WANG Le-feng GE yong-gui WANG Hong-shi xu Li NI Zhu-hua ZHANG Da-peng The Heart Center Beijing Chao Yang Hospital Capital MedicalUniversity Beijing 100020 China

Background Primary percutaneous coronary intervention （PCI） is the best treatment of choice for acute ST segment elevation myocardial infarction （STEMI）. This study aimed to determine the clinical outcomes of tirofiban combined with the low molecular weight heparin （LMWH）, dalteparin, in primary PCI patients with acute STEMI. Methods From February 2006 to July 2006, a total of 120 patients with STEMI treated with primary PCI were randomised to 2 groups： unfractionated heparin （UFH） with tirofiban （group I： 60 patients, （61.2±9.5） years）, and dalteparin with tirofiban （group il： 60 patients, （60.5±10.1） years）. Major adverse cardiac events （MACE） during hospitalization and at 4 years after PCI were examined. Bleeding complications during hospitalization were also examined. Results There were no significant differences in sex, mean age, risk factors, past history, inflammatory marker, or echocardiography between the 2 groups. In terms of the target vessel and vascular complexity, there were no significant differences between the 2 groups. During the first 7 days, emergent revascularization occurred only in 1 patient （1.7%） in group I. Acute myocardial infarction （AMI） occurred in 1 （1.7%） patient in group I and in 1 （1.7%） in group II. Three （5.0%） patients in group I and 1 （1.7%） in group II died. Total in-hospital MACE during the first 7 days was 4 （6.7%） in group I and 2 （3.3%） in group Ⅱ. Bleeding complications were observed in 10 patients （16.7%） in group I and in 4 patients （6.7%） in group II, however, the difference was not statistically significant. No significant intracranial bleeding was observed in either group. Four years after PCI, death occurred in 5 （8.3%） patients in group I and in 4 （6.7%） in group Ⅱ. MACE occurred in 12 （20.0%） patients in group I and in 10 （16,7%） patients in group Ⅱ. Conclusions Dalteparin was effective and safe in primary PCI of STEMI patients and combined dalteparin with tirofiban was effective and safe without significant bleedi

关键词： acute myocardial infarction, heparin primary percutaneous coronary intervention

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Hutong Tour in Beijing

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Women of China 1997年第3期 54-55页

作者： LI JIANGSHU and xu yong

Touring in rickshaws adds fun to the tour. The areas between the Drum Tower and Bell Tower are dense ly covered with thousands of one-story houses and hutongs.

关键词： Hutong Tour in Beijing

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