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Relationship between Vesicoureteral Reflux and Glomerular Filtration Rate in Children

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Current Medical Science 2020年第5期40卷 845-850页

作者： Wei ZHANG Hui-ming yi Xiao-le ZHANG yong-hong yi Jian-hua ZHOU Li-ru QIU Department of Ultrasound Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Vesicoureteral reflux(VUR)is one of the most common urinary tract anomalies in children and causes renal damage and studies focusing on the effect of VUR on renal function are *** recruited 35 primary VUR patients with recurrent urinary tract infection(UTI)and 10 non-VUR patients with recurrent ***-enhanced voiding urosonography(ceVUS)was performed for VUR grading,and renal dynamic imaging was used for evaluating glomerular filtration rate(GFR,mL/min).Standardized GFR(sGFR),namely GFR/BSA(mL·min-1·m-2),was calculated based on the body surface area(BSA).Total sGFR(tsGFR,mL·min-1·m-2)was obtained from the sum of sGFR on the left and right sides of all the *** risk of renal regurgitation was equal in the unilateral reflux *** sGFR of children with grade Ⅳ(45.74±18.05mL·min-1·m-2)and grade V(49.67±23.63mL·min-1·m-2)reflux was significantly lower than that in children with grade Ⅱ(77.69±22.21 mL·min-1·m-2).The renal function compensation of contralateral non-reflux kidney increased in unilateral reflux group,which was higher than that in the control group and level Ⅱ,Ⅳ and Ⅴ of reflux group *** VUR group of the same grade,sGFR decreased with the age at *** unilateral grade V refux group,the tsGFR was lower than that in the unilateral grade I reflux group(133.51±48.21 vs.186.87+53.49mL·min-1·m-2).The patients with VUR of unilateral grade Ⅱ were significantly older than those with VUR of unilateral grades Ⅱ and Ⅳ.This study indicates that severe VUR is significantly associated with decreased renal ***,VUR should be diagnosed early and managed individually.

关键词： vesicoureteral reflux renal dynamic imaging glomerular filtration rate renal damage children

Human transcription factor genes involved in neuronal development tend to have high GC content and CpG elements in the proximal promoter region

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Journal of Genetics and Genomics 2011年第4期38卷 157-163页

作者： Yue-Sheng Long Jia-Ming Qin Tao Su Qi-Hua Zhao yong-hong yi Wei-Ping Liao Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510260China

Transcription factors（TFs）play critical roles in the development of the nervous system,but the transcriptional regulatory mechanisms of these genes are poorly *** we analyzed 5-kb of the 5＇ flanking genomic DNA sequences of 41 TF genes involved in neuronal *** results showed that the TF genes tend to have higher GC contents in the proximal region and most of the TF genes have at least one proximal GC-rich（GC content60%）promoter with a CpG *** promoter distribution analysis showed that the GC-poor promoters were sporadically distributed within the 5-kb flanking genomic sequence（FGS）;however,more than half（37 of 70）of the GC-rich promoters were located in the proximal region between nucleotides—1 and—*** assays showed that partial GC-rich promoters increased gene expression in SH-SY5Y cells and that CpG methylation repressed the promoter *** study suggests a potential general mechanism for regulation of TF expression.

关键词： Transcription factor Promoter GC content CpG island DNA methylation

Ion Channel Genes and Epilepsy:Functional Alteration,Pathogenic Potential,and Mechanism of Epilepsy

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Neuroscience Bulletin 2017年第4期33卷 455-477页

作者： Feng Wei Li-Min Yan Tao Su Na He Zhi-Jian Lin Jie Wang yi-Wu Shi yong-hong yi Wei-Ping Liao Institute of Neuroscience Department of Neurology of The Second Affiliated Hospital of Guangzhou Medical UniversityKey Laboratory of Neurogenetics and Channelopathies of Guangdong Province and Ministry of Education of ChinaGuangzhou Medical University Department of Neurology Guangdong Second Provincial General Hospital

Ion channels are crucial in the generation and modulation of excitability in the nervous system and have been implicated in human epilepsy. Forty-one epilepsyassociated ion channel genes and their mutations are systematically reviewed. In this paper, we analyzed the genotypes, functional alterations（funotypes）, and phenotypes of these mutations. Eleven genes featured loss-offunction mutations and six had gain-of-function *** genes displayed diversified funotypes, among which a distinct funotype-phenotype correlation was found in SCN1A. These data suggest that the funotype is an essential consideration in evaluating the pathogenicity of mutations and a distinct funotype or funotype-phenotype correlation helps to define the pathogenic potential of a gene.

关键词： Epilepsy Ion channel gene Epilepsy gene Genetics Gene function Pathogenic mechanism

Proteomic analysis of the immune response of the silkworm infected by Escherichia coli and Bacillus bombyseptieus

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Insect Science 2012年第5期19卷 559-569页

作者： Xiao-Wu Zhong Ping Zhao yong Zou hong-yi Nie Qi-ying yi Qing-You Xia Zhong-Huai Xiang The Key Sericultural Laboratory of Agriculture Ministry Southwest University Chongqing China

he silkworm, Bombyx mori, is an economically important insect with a 5 000-year history of domestication. During evolution, the silkworm has developed highly effective defenses against invasion and parasitization by microorganisms. In this study, two microorganisms Escherichia coli and Bacillus bombyseptieus were orally infected to silk- worm larvae. After infection with E. coli and B. bombyseptieus for 24 h, we investigated the polypeptide changes in the hemolymph, midgut and integument using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time of flight mass spec- trometry. Forty-seven differentially expressed proteins were identified in these tissues. They belonged to a variety of functional classes, including immune proteins, metabolic proteins and structural proteins. Compared with controls, E. coli-infected silkworms showed 21 up- regulated proteins, 25 down-regulated proteins and lost one protein. After infection with B. bombyseptieus, silkworms showed 15 up-regulated proteins, 27 down-regulated pro- teins, lost three proteins and retained two proteins unchanged. We speculate that all these proteins may play a role in the silkworm immune response, although it is unclear why and how the two kinds of bacteria can so markedly alter expression of these proteins. These re- sults offer valuable insights for measuring the proteomic responses of the silkworm innate immune mechanism.

关键词： Bacillus bombyseptieus Escherichia coli immune response proteomics silkworm

A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level

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Neuroscience Bulletin 2013年第3期29卷 348-354页

作者： Guang-Fei Deng Shu-Jing Liu Xun-Sha Sun Wei-Wen Sun Qi-Hua Zhao Wei-Ping Liao yong-hong yi Yue-Sheng Long Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes Guangzhou Medical University

LIM kinase 1 （LIMK1）, a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI （8.0） software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt ＋884 to ＋966 in the human LIMK1 3＇ untranslated region （UTR） was highly conserved in the mouse Limkl 3＇ UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3＇ UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3＇-UTR mutant and neuroloqical disorders.

关键词： LIM kinase 1 region post-transcriptsecondary structurethree-prime untranslatedonal regulation RNA

Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells

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Acta Pharmacologica Sinica 2005年第2期26卷 223-227页

作者： Jing LIU,Qing-li WU,yong-hong FENG,yi-fu YANG,Xiao-yu LI,Jian-ping ZUO~2State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academyof Sciences,Shanghai 201203,China State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:To investigate the effects of triptolide,a diterpenoid triepoxide fromTripterygium wilfordii Hook F(TWHF),on the co-stimulatory molecule expres-sion and interleukin-12(IL-12)production from THP-1 ***:THP-1 cellswere differentiated into macrophage-like cells by Me2SO,and then cultured withIFN-γ(500kU/L)and lipopolysaccharide(LPS)(1mg/L)with or without *** surface molecule expressions were analyzed on a FACScan flow ***-12p40,IL-12p70 were assayed by ***:Tripolide suppressed CD80and CD86 expressions on IFN-γ(500kU/L)and LPS(1mg/L)activated THP-1 cellsat nontoxic dosages of 2.5-0.625μg/***,the production of IL-12p40and IL-12p70 were also significantly reduced in THP-1 cells exposed to ***:Triptolide impairs the antigen-presenting function by inhibiting CD80and CD86 expressions and decreased IL-12p40 and IL-12p70(bioactive form)pro-ductions from the activated THP-1 cells.

关键词： triptolide CD80 antigens CD86 antigens interleukin-12

Expression of microRNA-134 and Limk1 in brain tissue of FMR1 knockout mice

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Expression of microRNA-134 and Limk1 in brain tissue of FMR1...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： yong-hong yi~*,Zhi-yong ZENG,Wei-Wen SUN,Sheng-Qiang CHEN,Yue-Sheng LONG,yi-Wu SHI,Wei-Ping LIAO Institute of Neuroscience,Guangzhou Medical College,Guangzhou 510260

Objective Fragile X syndrome(FXS) is a common inherited mental retardation caused by CGG trinucleotide expansion in the 5’untranslated region of the FMR1 *** significant neuropathologic lesion associated with FXS is abnormally thin and tortilis dendritic immature *** FMR1 product FMRP might contribute to the function of miRNAs as one of component of RNA-induced silencing complex(RISC).The present study is to explore whether the loss of FMRP causes the dysfunction of miR-134 silencing Limk1mRNA related to development of dendritic *** FVB strain male FMR1 KO mice and their wild type(WT) counterparts were *** immunohistochemistry method was used to examine the expression of Limk 1 and qRT-PCR was used to determine the transcriptional level of *** (1)The mean optical density and positive cell count of Limk1 in each brain regions of KO,K0 and KO group are significantly higher than the age-matched WT group(P0.05).(3)The transcriptional level of miR-134 was significantly decreased in both 6 week KO and WT mice when compared with the newborn and 2 week same genotype mice(P<0.05).Conclusion The increased Limk1 level in KO mice demonstrates that FMRP down-regulates the expressions of Limk 1,which might contribute to the abnormal development of dentritic spine in *** loss of FMRP do not affect the level of miR-134 It is needed to study further whether the increased Limk1 is caused by dysfunction of miR- 134.

关键词： fragile X syndrome LIM-kinase 1(Limk1) microRNA-134(miR-134) fragile X mental retardation protein dentritic spine

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《全清词》失收粤人词作49首

汕头大学学报（人文社会科学版） 2023年第3期39卷 14-19,94页

作者：杨洪艺湘潭大学文学与新闻学院湖南湘潭411100

《全清词·顺康卷》和《全清词·雍乾卷》是收录清代顺治、康熙、雍正、乾隆四朝共150多年词作的总集,其力求全面和完备,以反映该时段词创作全貌,但因为种种原因而仍有失收。载于清代词总集和别集等文献,出自梁无技、易宏、李继燕、陶... 详细信息

《全清词·顺康卷》和《全清词·雍乾卷》是收录清代顺治、康熙、雍正、乾隆四朝共150多年词作的总集,其力求全面和完备,以反映该时段词创作全貌,但因为种种原因而仍有失收。载于清代词总集和别集等文献,出自梁无技、易宏、李继燕、陶鍹、卢作樑、黄呈兰等6位广东词人的49阕词作未见于《全清词》,今予以辑补,同时简略考察作者的生平事迹、词创作等相关情况。

关键词：《全清词·顺康卷》《全清词·雍乾卷》辑佚

Spectrum of SCN1A mutations in Chinese patients with febrile seizures related epilepsy

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Spectrum of SCN1A mutations in Chinese patients with febrile...