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Streamlined whole-genome genotyping through NGS-enhanced thermal asymmetric interlaced(TAIL)-PCR

Plant Communications 2024年第9期5卷 25-37页

作者： Sheng zhao Yue Wang Zhenghang Zhu Peng Chen Wuge Liu Chongrong Wang Hong Lu yong Xiang Yuwen Liu Qian Qian Yuxiao chang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China 2 Guangdong Provincial Key Laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China

Whole-genome genotyping(WGG)stands as a pivotal element in genomic-assisted plant ***theless,sequencing-based approaches for WGG continue to be costly,primarily owing to the high expenses associated with library preparation and the laborious *** prior development of foreground and background integrated genotyping by sequencing(FBI-seq),we discovered that any sequence-specific primer(SP)inherently possesses the capability to amplify a massive array of stable and reproducible non-specific PCR products across the ***,we further improved FBI-seq by replacing the adapter ligated by Tn5 transposase with an arbitrary degenerate(AD)*** protocol for the enhanced FBI-seq unexpectedly mirrors a simplified thermal asymmetric interlaced(TAIL)-PCR,a technique that is widely used for isolation of flanking ***,the improved TAIL-PCR maximizes the primer-template mismatched annealing capabilities of both SP and AD *** addition,leveraging of next-generation sequencing enhances the ability of this technique to assay tens of thousands of genome-wide loci for any *** cost-effective,user-friendly,and powerful WGG tool,which we have named TAIL-PCR by sequencing(TAIL-peq),holds great potential for widespread application in breeding programs,thereby facilitating genome-assisted crop improvement.

关键词： whole-genome genotyping primer-template mismatched annealing specific primer arbitrary degenerate primer molecular breeding

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Progress in in-situ electrochemical nuclear magnetic resonance for battery research

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Magnetic Resonance Letters 2024年第2期4卷 13-21页

作者： yong Jiang Mengmeng zhao Zhangquan Peng Guiming Zhong Laboratory of Advanced Spectro-electrochemistry and Li-ion Batteries Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China University of Chinese Academy of Sciences Beijing100049China Tianmu Lake Institute of Advanced Energy Storage Technologies Co. Ltd.Liyang213300China

A thorough understanding of the fundamental electrochemical and chemical processes in batteries is crucial to advancing energy density and power ***,the characterizations of such processes are ***-situ electrochemical nuclear magnetic resonance(EC-NMR)offers the capability to collect real-time data during battery operation,furnishing insights into the local structures and ionic dynamics of materials by monitoring changes in the chemical environment around the ***-NMR also has the advantages of being both quantitative and *** paper systematically reviews the design of EC-NMR approach,and delves into the applications and progress of EC-NMR concerning battery reaction mechanisms,failure mechanisms,and overall battery *** review culminates in a comprehensive summary of the perspective and challenges associated with EC-NMR.

关键词： In-situ NMR Reaction mechanism Failure mechanism Battery systems Ionic dynamics

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Optimal V_(2)O_(3) extraction by sustainable vanadate electrolysis in molten salts

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Tungsten 2024年第1期6卷 141-149页

作者： Bao-Yan Feng Zhong-Hua zhao yong-Zheng Jia Jia-Liang An Ming-yong Wang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China Beijing Key Laboratory of Green Recovery and Extraction of Rare and Precious Metals University of Science and Technology BeijingBeijing 100083China

Recently,an environmentally friendly electrolysis process of soluble vanadates is proposed and successfully confirmed for V_(2)O_(3) *** this paper,the solubilities of various vanadates(***_(3),Na_(4)V_(2)O_(7) and Na_(3)VO_(4))in NaCl molten salt are *** dependences of V_(2)O_(3) extraction on vanadate form,anode material and molten salt system are studied.A long-term electrolysis is carried *** result indicates that the solubilities of all vanadates are high and meet electrolytic *** to Na_(4)V_(2)O_(7) and Na_(3)VO_(4),NaVO_(3) exhibits larger current efficiency and lower electricity *** using SnO_(2) anode instead of graphite anode,the current efficiency of NaVO_(3) electrolysis can be increased to 58.1%and carbon pollution is avoided at 700℃.The extraction rate of V_(2)O_(3) reaches 81.3%after long-term electrolysis of 51 *** work optimizes the electrochemical preparation process of V_(2)O_(3) from various vanadates and contributes to the improvement of current efficiency and extraction rate.

关键词： Molten salt electrolysis Vanadate Vanadium trioxide Current efficiency

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A novel Ce_(0.485)Zr_(0.485)Y_(0.03)O_(2) composite oxide with surface doping of Y and its application in Pd-only three-way catalyst

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Rare Metals 2024年第2期43卷 749-757页

作者： Zheng zhao Wei-Xin zhao yong-Qi Zhang Mei-Sheng Cui yong-Ke Hou Dong-Ming Chen Juan-Yu Yang Zong-Yu Feng Xiao-Wei Huang National Engineering Research Center for Rare Earth Grirem Advanced Materials Co.Ltd.GRINM Group Corporation LimitedBeijing100088China Griem Hi-Tech Co.Ltd. Langfang065000China Rare Earth Functional Materials(Xiong’an)Innovation Venter Co.Ltd. Baoding071700China

The ceria-zirconia compound oxide-supported noble metal Pd(Pd@CZ)is widely used in three-way ***,the surface structure of CZ plays an important role in catalytic activity of ***,how to regulate the surface structure of CZ and clarify the structure–activity relationship is still a *** this paper,a strategy is proposed to develop high activity Pd@CZ nanocatalysts by tuning Y doping sites in *** precipitate-deposition method is developed to prepare the novel Ce_(0.485)Zr_(0.485)Y_(0.03)O_(2) composite with surface doping of Y(CZ-Y-S).In addition,the Pd@CZ-Y-S(Pd supported on CZ-Y-S)exhibits superior catalytic activity for HC,CO,and NO oxide,wherein,for CO and C_(3)H_(6) oxidation,the low-temperature activity of Pd@CZ-Y-S is still 20%higher than that of Pd@CZ-Y-B(Y bulk doping)and commercial Pd@CZ after 1000℃/4 h *** effect mechanism is further studied by density functional theory(DFT)*** with Pd@CZ-Y-B,Pd@CZ-Y-S shows the lower CO oxide reaction energy barriers due to the weaker adsorption strength of *** Y surface doping strategy could provide valuable insights for the development of highly efficient Pd@CZ catalyst with extensive applications.

关键词： Rare earth Ceria-zirconia-supported Pd Surface doping Experiment and theory Catalytic application

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Tuning interface mechanism of FeCo alloy embedded N,S-codoped carbon substrate for rechargeable Zn-air battery

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Journal of Energy Chemistry 2024年第6期93卷 400-410,I0010页

作者： Hui chang Lulu zhao Shan zhao Zong-Lin Liu Peng-Fei Wang Ying Xie Ting-Feng Yi School of Materials Science and Engineering Northeastern UniversityShenyang 110819LiaoningChina Key Laboratory of Functional Inorganic Material Chemistry Ministry of EducationSchool of Chemistry and Materials ScienceHeilongjiang UniversityHarbin 150080HeilongjiangChina Key Laboratory of Dielectric and Electrolyte Functional Material Hebei Province School of Resources and MaterialsNortheastern University at QinhuangdaoQinhuangdao 066004HebeiChina

The interface mechanism between catalyst and carbon substrate has been the focus of *** this paper,the FeCo alloy embedded N,S co-doped carbon substrate bifunctional catalyst(FeCo/S-NC)is obtained by a simple one-step pyrolysis *** experimental results and density functional theory(DFT)calculation show that the formation of FeCo alloy is conducive to promoting electron transfer,and the introduction of S atom can enhance the interaction between FeCo alloy and carbon substrate,thus inhibiting the migration and agglomeration of particles on the surface of carbon *** FeCo/SNC catalysts show outstanding performance for oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).FeCo/S-NC shows a high half-wave potential(E_(1/2)=0.8823 V)for ORR and a low overpotential at 10 mA cm^(-2)(E_(j=10)=299 mV)for *** addition,compared with Pt/C+RuO_(2) assembled Zn-air battery(ZAB),the FeCo/S-NC assembled ZAB exhibits a larger power density(198.8 mW cm^(-2)),a higher specific capacity(786.1 mA h g_(zn)~(-1)),and ultra-stable cycle *** results confirm that the optimized composition and the interfacial interaction between catalyst and carbon substrate synergistically enhance the electrochemical performance.

关键词： FeCo alloy N S co-doped carbon DFT calculation Zn-air batteries Interfacial interaction

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Regional Climate Damage Quantification and Its Impacts on Future Emission Pathways Using the RICE Model

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Advances in Atmospheric Sciences 2024年第9期41卷 1843-1852页

作者： Shili YANG Wenjie DONG Jieming CHOU yong ZHANG Weixing zhao Beijing Meteorological Observation Centre Beijing Meteorological BureauBeijing 100089China School of Atmospheric Sciences Sun Yat-sen UniversityZhuhai 519082China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519082China Beijing Normal University Beijing 100101China CMA Institute for Development and Programme Design Beijing 100081China

This study quantified the regional damages resulting from temperature and sea level changes using the Regional Integrated of Climate and Economy(RICE)model,as well as the effects of enabling and disabling the climate impact module on future emission *** highlight varied damages depending on regional economic development and ***,China and Africa could suffer the most serious comprehensive damages caused by temperature change and sea level rise,followed by India,other developing Asian countries(OthAsia),and other high-income countries(OHI).The comprehensive damage fractions for China and Africa are projected to be 15.1%and 12.5%of gross domestic product(GDP)in 2195,with corresponding cumulative damages of 124.0 trillion and 87.3 trillion United States dollars(USD)from 2005 to 2195,***,the comprehensive damage fractions in Japan,Eurasia,and Russia are smaller and projected to be lower than 5.6%of GDP in 2195,with cumulative damages of 6.8 trillion,4.2 trillion,and 3.3 trillion USD,***,coastal regions like Africa,the European Union(EU),and OHI show comparable damages for sea level rise and temperature *** China,however,sea level-induced damages are projected to exceed those from temperature ***,this study indicates that switching the damage modules on or off affects the regional and global emission trajectories,but the magnitude is relatively *** 2195,global emissions under the experiments with all of the damage modules switched off,only the sea level damage module switched on,and only the temperature damage module switched on,were 3.5%,2.3%and 1.2%higher than those with all of the damage modules switched on,respectively.

关键词： climate damage integrated assessment model carbon emissions sea level rise temperature change

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Spectral Selectivity of CdTe Cells with Substrate Configuration for Photovoltaic/Thermal Applications

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Journal of Thermal Science 2024年第4期33卷 1542-1553页

作者： CHEN Ken HU Kongfu zhao Bin CHEN Tao HAO yong PEI Gang Department of Thermal Science and Energy Engineering University of Science and Technology of ChinaHefei230027China Key Laboratory of Solar Thermal Conversion of Anhui Province Hefei230027China Hefei National Laboratory for Physical Sciences at Microscale Department of Materials Science and EngineeringUniversity of Science and Technology of ChinaHefei230026China Institute of Engineering Thermophysics Chinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences Beijing100049China

Existing photovoltaic cells with high infrared emissivity generate huge radiative heat loss in photovoltaic/thermal applications and degrade the photothermal *** purpose of this work is to evaluate the full spectral absorptivity of CdTe cells to find a spectrally selective photovoltaic cell for photovoltaic/thermal *** this end,the solar absorptivity and mid-infrared thermal emissivity of CdTe cells were tested by ellipsometry,UV-Vis-NIR spectrophotometer,and Fourier transform infrared *** experimental results show that the AM 1.5 solar spectrum weighted absorptivity of the substrate configuration CdTe cell reaches 0.91,and the mid-infrared emissivity is only 0.29,while the superstrate configuration cell emissivity is as high as *** research shows that substrate configuration with a transparent conductive layer on top can be flexibly grown on metal foils and has spectral selectivity with high solar absorptivity and low mid-infrared emissivity should be considered in the future for photovoltaic/thermal applications.

关键词： photovoltaic/thermal spectral selectivity substrate configuration CdTe cell ITO

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Spatial Equity Evaluation of Rural Eldercare Service Resources Based on Accessibility: A Case Study of Huanxian County of Gansu Province, China

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Chinese Geographical Science 2024年第5期34卷 869-885页

作者： chang Xiaoyan MA Libang CUI Xijuan TAO Tianmin zhao Shoucun College of Geography and Environmental Science Northwest Normal UniversityLanzhou 730000China Key Laboratory of Resource Environment and Sustainable Development of Oasis Gansu ProvinceLanzhou 730070China Northwest Institute of Urban-Rural Development and Collaborative Governance Lanzhou 730070China

Safeguarding the elderly population in the countryside is an inherent requirement for alleviating social conflicts in rural areas and effectively carrying out China’s Rural *** existing spatial mismatch between rural elderly service facilities and pop-ulation distribution in China aggravates the imbalance of facility resource space,how to improve the accuracy of demand and supply results and better measuring the spatial fairness of elderly service is significant to realize the optimal allocation of rural elderly *** on the above,this study pays attention to the refined needs of the elderly population,and focuses on the spatial equity of the rural elderly facilities,to make up for the short boards of the basic public services in the countryside and realize the all-round rural *** study takes Huanxian County in the hilly loess area of Longdong,China as an example and explores the spatial equity of rural elderly services by using the Urban Spatial Network Analysis(UNA)to measure the accessible quantity and supply capacity of elderly *** results found that there were 553 rural eldercare service facilities in Huanxian County,including 285 eldercare facilities and 268 medical and health facilities,and the spatial distribution is characterized by a southward shift in the east and uneven regional ***,the supply capacity of rural pension services was generally good,with the township center as the gathering point and spreading out in a faceted manner,with nearby townships forming a contiguous area;however,there were strong contrasts and obvious differences in the strength of supply ***,the spatial equity of rural eldercare service was *** 42.05%of the eld-erly population grid cells,eldercare service spatial equity was extremely *** spatial distribution also presented a northern preference over the southern,a western preference over the eastern,and a concentration along the *** stud

关键词： rural eldercare service spatial equity hilly loess region of Longdong Huanxian County China

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Broadband absorption performance of 3D-printed polyetheretherketone-based electromagnetic wave-absorbing composites

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Chinese Journal of Aeronautics 2024年第8期37卷 547-558页

作者： Lin BAI Nan chang Meiying zhao Chi HOU Yi CAO Dichen LI School of Aeronautics Northwestern Polytechnical UniversityXi’an 710072China Chengdu Aircraft Design and Research Institute Chengdu 610091China Xi’an Key Laboratory of Advanced Structure Design and Application of Aircraft Northwestern Polytechnical UniversityXi’an 710072China State Key Laboratory for Manufacturing System Engineering Xi’an Jiaotong UniversityXi’an 710054China

The rapid development of additive manufacturing technology has offered a new avenue for designing and fabricating high wave-absorbing meta *** this study,the mechanical properties and broadband absorption performance of Poly-Ether-Ether-Ketone(PEEK)–based electromagnetic wave–absorbing composite materials was *** high-performance polymer PEEK was used as the matrix,and the materials with electromagnetic wave loss,such as reduced graphene oxide,Carbonyl Iron(CI),and Flake CI(FCI),were used as *** on the theory of impedance matching,a wave-absorbing structure with a gradual impedance gradient was designed and *** test results showed that at the 2.0–18.0 GHz frequency band,the coverage rate of the effective absorption bandwidth was up to 72.0%,the average optimal reflectivity was–18.09 dB,and the wide-angle absorption range was 0°–30°.The advantages of additive manufacturing technology in designing and fabricating wave-absorbing structures are presented,demonstrating that the technology is an effective method for creating broadband absorbing structures.

关键词： Absorption performance Additive manufacturing Electromagnetic absorption PEEK composite materials Gradual structure Broadband absorption

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Identification of Motor Nuclei in the Medulla Oblongata of Carp for Biological Control

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Journal of Bionic Engineering 2024年第2期21卷 842-851页

作者： Yang zhao yong Peng Yudong Wen Lingjun Han Yanhong Yan Xueying Dong Hui Zhang Zheng zhao Xiaoyue Liu School of Electrical Engineering Yanshan UniversityQinhuangdao066004China School of Mechanical Engineering Yanshan UniversityQinhuangdao066004China Key Laboratory of National Defense of Mechanical Structure and Material Science Under Extreme Conditions Yanshan UniversityQinhuangdao066004China Institute of Marine Science and Engineering Yanshan UniversityQinhuangdao066004China Shijiazhuang University Shijiazhuang050035China

Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the *** purpose of this study was to explore the motor nuclei of the medulla oblongata in *** carps were subjected to electrical stimulation and chemical stimulation experiments,respectively,in the water-free state,and the effective chemical stimulation sites were injected with the pontamine sky blue *** brain tissue sections were obtained by paraffin tissue section technology and the neutral red staining *** comparing the positions of the brain nuclei shown in earlier studies,the motor nerve nuclei in the medulla oblongata were *** brain electrode was implanted into the motor nucleus of the medulla oblongata,and the underwater control experiment and behavioral tests were carried out with different electrical stimulation *** results showed that the abducens nucleus(NVI)was the motor nucleus that controls the ipsilateral steering,and the facial nucleus(NVII)was the motor nucleus that controls the forward *** adjusting the stimulation voltage and the stimulation pulse number,the carp can be stably controlled to achieve a left–right steering motion of 30°–180°and a forward motion of more than 80 cm/*** study indicated that the quantitative control of the steering and forward behavior of the carp can be achieved by electrical stimulation of the NVI and NVII,which provided a certain experimental basis for the accurate control of the carp robot.

关键词： Biological control Carp Medulla oblongata motor nucleus Electrical stimulation technique Chemical stimulation technique

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