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Induction of Apoptosis by L-NMMA,via FKHRL1/ROCK Pathway in Human Gastric Cancer Cells

Biomedical and Environmental Sciences 2006年第4期19卷 285-291页

作者： yong-zhong wang ZHEN-QING FENG Department of Pathology Nanjing Medical University Nanjing 210029 Jiangsu China

Objective To investigate the apoptosis-inducing effect of endogenous nitric oxide （NO） suppression in gastric cancer cells and its mechanisms. Methods Apoptosis of gastric cancer cells was detected by flow cytometry. Expression of phosphorylated FKHRL1 （thr-32, ser-253） and FKHRL1 in gastric cancer cells was analyzed using Western blotting. Immunofluorescence assay was performed to localize the intracellular phosphorylated FKHRL1 （thr-32, ser-253） and FKHRL 1. Transfection of FKHRL1-HA wild type and mutant FKHRL1-HA T32A constructs was performed by lipofectamine plus reagent. NO generation was determined by Griess reaction. Results Gastric cancer cells were significantly apoptotic after treatment with NG-monomethyl-L-arginine （L-NMMA, a nitric oxide synthase inhibitor）, compared with the control （P〈0.01）. The apoptosis of gastric cancer cells induced by L-NMMA was dose-dependent and time-independent. However, the Z-DEVD-fmk, a caspase-3, 6, 7, 8, l0 inhibitor, did not prevent the apoptosis. The immunofluorescence assays showed that FKHRL1 protein was strongly expressed in the nucleu and p-FKHRL1 thr-32 protein was ：strongly expressed in the cytoplasm of SGC-7901 cells when endogenous nitric oxide generation was blocked by L-NMMA, but no change in FKHRL1 ser-253 phosphorylation. Nevertheless, ROCK protein was strongly expressed in p-FKHRL1 thr-32-positive SGC-7901 cells. The wortmannin, an inhibitor of phosphoinositol-3-OH kinase （PI3K）, did not block the phosphorylated FKHRL1 thr-32 protein induced by L-NMMA. However, Y-27632, a specific inhibitor of the protein kinase ROCK, significantly blocked apoptosis induced by phosphorylated FKHRL1 thr-32 （P〈0.01）, which was mediated by L-NMMA. A significant decrease in NO generation （P〈0.01） and a significant increase in apoptosis （P〈0.01） were observed when FKHRL1-HA wild-type cells were transfected, which caused increased FKHRL1 thr-32 phosphorylation. Conclusions L-NMMA triggers gastric carcinoma cell apoptosis, possibly

关键词： Gastric carcinoma Nitric oxide FKHRL1 Apoptosis Signaling

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Local Uniqueness of Weak Solutions for a Class of Quasilinear Subelliptic Equations

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Journal of Mathematical Research and Exposition 2011年第2期31卷 295-302页

作者： Xue Wei CUI yong zhong wang Department of Applied Mathematics Northwestern Polytechnical University Shaanxi 710072 P. R. China

In this note,we obtain some a-priori estimates for gradient of weak solutions to a class of subelliptic quasilinear equations constructed by Ho¨rmander’s vector fields,and then prove local uniqueness of weak solutio... 详细信息

关键词： Hrmander’s vector fields subelliptic weak solution uniqueness

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Oligonucleotide chip, real-time PCR and sequencing for genotyping of hepatitis B virus

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World Journal of Gastroenterology 2007年第31期13卷 4260-4263页

作者： yong-zhong wang Guo-Xiang Wu Li-Bo Luo Min Chen Li-Hua Ruan Research Center for Liver diseases The ThirdHospital of Changzhou Changzhou 213001 Jiangsu ProvinceChina

To compare the oligonucleotide chip, real-time PCR and sequencing for genotyping of hepatitis B virus in Chinese patients with chronic hepatitis B. METHODS： Mixture of samples with different genotypes and clinical serum samples from 126 chronic hepatitis B patients was tested for hepatitis B virus genotypes by oligonucleotide chip, real-time PCR and sequencing of PCR products, respectively. Clinical performances, time required and costs of the three assays were evaluated. RESULTS： Oligonucleotide chips and real-time PCR detected 1% and 0.1% genotypes, respectively, in mixed samples. Of the 126 clinical samples from patients with chronic hepatitis B, genotype B was detected in 41 （33%）, 41 （33%） and 45 （36%） samples, and genotype C in 76 （60%）, 76 （60%） and 81 （64%） samples, by oligonucleotide chip, real-time PCR and sequencing, respectively. Oligonucleotide chip and real-time PCR detected mixed genotypes B and C in 9 samples. Real- time PCR was the rapidest and cheapest among the three assays. CONCLUSION： Oligonucleotide chip and real-time PCR are able to detect mixed genotypes, while sequencing only detects the dominant genotype in clinical samples.

关键词： Hepatitis B virus Genotypes Oligonucleotidechips Real-time PCR Sequencing

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Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China

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World Journal of Gastroenterology 2007年第13期13卷 1975-1979页

作者： Xiang-Rong Tang Ji-Shen Zhang Hui Zhao Yu-Hua Gong yong-zhong wang Jian-Long Zhao Shanghai Institute of Microsystem and Information Technology CAS Shanghai 200050 China The Third People's Hospital of Zhenjiang Zhenjiang 212003 Jiangsu Province China The Third People's Hospital of Changzhou Changzhou 213001 Jiangsu Province China

AIM： TO determine the genotype distribution of hepatitis B virus （HBV） with a newly oligonucleotide chip assay among the HBV carriers in Eastern China. METHODS： An assay using oligonucleotide chip was developed for detection of HBV genotypes in serum samples from HBV DNA-positive patients in Eastern China. This method is based on the principle of reverse hybridization with Cy5-labeled amplicons hybridizing to type-specific oligonucleotide probes that are immobilized on slides. The results of 80 randomly chosen sera were confirmed by direct sequencing. RESULTS： HBV genotype B, C and mixed genotype were detected in 400 serum samples, accounting for 8.3% （n = 33）, 83.2% （n = 333）, and 8.5% （n = 34）, respectively. The evaluation of the oligonucleotide assay showed 100% concordance with the amplicon phylogenetic analysis except 9 mixed genotype infections undetected by sequencing. CONCLUSION： The study indicates that HBV genotype C and B prevail in the Eastern China. It is suggested that the oligonucleotide chip is a reliable and convenient tool for the detection of HBV genotyping.

关键词： Oligonucleotide chip Hepatitis B virus Genotype

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Influence of Gd doping on the absolute quantum efficiency and lifetime of Eu_xGd_(1-x)(TTA)_3 phens

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Chinese Chemical Letters 2014年第6期25卷 892-896页

作者： Ting-Ting Dai Lei Liu Dong-Liang Tao Shi-Gang Li HongZhang Yu-Min Cui yong-zhong wang Ji-Tang Chen Kun Zhang Wen-zhong Sun Xiao-Yun Zhao gel Liu College of Chemistry and Chemical Industry Fuyang Normal College Fuyang 236041 China Anhui Provincial Key Laboratory for Degradation and Monitoring of Pollution of the Environment Fuyang 236041 China School of Physics and Electronic Science Fuyang Normal College Fuyang 236041 China

Absolute quantum yield （Ф） is one of the most important parameters to evaluate the potential of novel materials. Lanthanide complexes EuxGd1-x（TTA）3phens are synthesized with the ratio of Gd3＋ dopant concentration ranging from 10% to 90% to improve the absolute quantum yield. EuxGd1-x（TTA）3phens possess similar infrared and ultraviolet spectra, showing that they have similar molecular structures. The absolute emission quantum yields of EuxGd1-x（TTA）3phens are determined using a fluoromax-4 spectrofluorometer equipped with an integrating sphere. The fluorescence lifetimes of the EuxGd1-x（TTA）3phens are measured in the same experiment. It was found that both absolute quantum yields and fluorescence lifetimes of EuxGd1-x（TTA）3phens are of quasi-periodic variation with the change of the Gd3. dopant concentrations. The absolute quantum efficiency and fluorescence lifetime vary with respect to the Gd content in an opposite fashion, indicating that the rate of energy absorption by the EuxGd1-x（TTA）3phens and the conversion to light energy is critical for the absolute quantum efficiency. The radiative rate constant Kr and non-radiative rate constant Knr are calculated. The dependence of Kr and Knr on the Gd3＋ dopant concentrations is very similar to that of absolute quantum efficiency. The radiation rate constant Kr and absolute quantum efficiency have a linear relationship.

关键词： JEuropium complexes Gd doping Absolute quantum yield Fluorescent lifetime

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The Hilbert expansion of the Boltzmann equation in the incompressible Euler level in a channel

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Science China Mathematics 2025年第1期68卷 39-88页

作者： Feimin Huang Weiqiang wang yong wang Feng Xiao Academy of Mathematics and Systems Science Chinese Academy of Sciences School of Mathematical Sciences University of Chinese Academy of Sciences Key Laboratory of Computing and Stochastic Mathematics (Ministry of Education) School of Mathematics and StatisticsHunan Normal University

The study of the hydrodynamic limit of the Boltzmann equation with physical boundary is a challenging problem due to the appearance of the viscous and Knudsen boundary *** this paper,the hydrodynamic limit from the Boltzmann equation with the specular reflection boundary condition to the incompressible Euler equations in a channel is *** on the multi-scaled Hilbert expansion,the equations with boundary conditions and compatibility conditions for interior solutions,and viscous and Knudsen boundary layers are derived under different scaling,***,some uniform estimates for the interior solutions,and viscous and Knudsen boundary layers are *** the help of the L2-L∞framework and the uniform estimates obtained above,the solutions to the Boltzmann equation are constructed by the truncated Hilbert expansion with multiscales,and hence the hydrodynamic limit in the incompressible Euler level is justified.

关键词： Boltzmann equation incompressible Euler equations hydrodynamic limit Hilbert expansion specular reflection boundary condition viscous boundary layer Knudsen boundary layer

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Dative B–N bonds weaven toward two-dimensional purely organic materials for single crystals

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Science China Chemistry 2025年第1期68卷 12-14页

作者： Wei-Lei Zhou Siwei wang yong Chen College of Chemistry and Materials Science Inner Mongolia Key Laboratory of Chemistry for Nature Products and Synthesis for Functional Molecules Innovation Team of Optical Functional Molecular Devices Inner Mongolia Minzu University College of Chemistry Nankai University

Molecular topology is one of the important topics to study and understand the relationship between molecular structure and properties [1]. Particularly, two-dimensional (2D) materials with extremely high surface area and ultra-thin morphology have attracted considerable attention due to their unexpected properties in materials and chemistry [2,3].

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Diagnosis and treatment of biliary atresia: a retrospective study

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 108-112页

作者： Shao-Tao Tang, Qing-Lan Ruan, Zhi-Qing Cao, yong-zhong Mao, yong wang and Shi-wang Li Wuhan, China Department of Pediatric Surgery, Wuhan Union Hos- pital, Huazhong Science and Technology University Wuhan 430022, China Department of Pediatric Surgery Wuhan Union Hospital Huazhong Science and Technology University Wuhan 430022 China. tshaotao83@***

BACKGROUND: Biliary atresia (BA) is the end results of an inflammatory process, which affect the intrahepatic and extrahepatic bile ducts, leading to fibrosis and obliteration of the biliary tract with the development of biliary cirrho- sis. Early diagnosis of BA is difficult, and there is no speci- fic therapy for BA at present. The purpose of this study was to investigate the diagnosis, treatment and postoperative outcome of BA and to explore new diagnostic and thera- peutic strategies. METHODS; Thirty-one children with biliary atresia from our hospital and 201 children with such disease from other hospitals in China were reviewed retrospectively in terms of diagnostic modalities, operative age, operative methods and long-term survival rates after operation. RESULTS : The operative age of the 31 patients varied from 13 to 270 days. It was 120 days in 3 ( 10% ). No children underwent transplantation for BA. Their 1-6 year survival rate on average was 32. 3% (10/31). The early operative rate in China was 12.9% (30/ 232). One child received liver transplantation with a long- term survival rate of 31.9%. CONCLUSIONS; Early recognition of babies with BA is critical for optimal intervention for preventing progressive fibrosis. Clinical presentation, imaging and laparoscopy are helpful in enhancing the early diagnostic rate of BA pa- tients. The long-term survival rates can be obtained after the improvement of operative methods, liver transplanta- tion for children with advanced BA, and development of other medical strategies.

关键词： biliary atresia diagnosis treatment strategies

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Industrial Control Anomaly Detection Based on Distributed Linear Deep Learning

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Computers, Materials & Continua 2025年第1期82卷 1129-1150页

作者： Shijie Tang yong Ding Huiyong wang School of Computer Science and Information Security Guilin University of Electronic TechnologyGuilin541004China School of Electronic Engineering and Automation Guilin University of Electronic TechnologyGuilin541004China Guangxi Engineering Research Center of Industrial Internet Security and Blockchain Guilin University of Electronic TechnologyGuilin541004China Institute of Cyberspace Technology HKCT Institute for Higher EducationHong Kong999077China School of Mathematics&Computing Science Guilin University of Electronic TechnologyGuilin541004China

As more and more devices in Cyber-Physical Systems(CPS)are connected to the Internet,physical components such as programmable logic controller(PLC),sensors,and actuators are facing greater risks of network attacks,and fast and accurate attack detection techniques are *** key problem in distinguishing between normal and abnormal sequences is to model sequential changes in a large and diverse field of time *** address this issue,we propose an anomaly detection method based on distributed deep *** method uses a bilateral filtering algorithm for sequential sequences to remove noise in the time series,which can maintain the edge of discrete *** use a distributed linear deep learning model to establish a sequential prediction model and adjust the threshold for anomaly detection based on the prediction error of the validation *** method can not only detect abnormal attacks but also locate the sensors that cause *** conducted experiments on the Secure Water Treatment(SWAT)and Water Distribution(WADI)public *** experimental results show that our method is superior to the baseline method in identifying the types of attacks and detecting efficiency.

关键词： Anomaly detection CPS deep learning MLP(multi-layer perceptron)

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溶液法CH3NH3PbCl3单晶的生长与性能研究

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无机材料学报 2016年第10期31卷 1063-1067页

作者：王万富苏静雷勇仲坤王迪南京信息工程大学物理与光电工程学院南京210044 固体微结构物理国家重点实验室人工微结构科学与技术协同创新中心南京210093

分别采用蒸发结晶法和逆温结晶法生长尺寸约为4 mm′3 mm′3 mm的CH_3NH_3PbCl_3单晶。对两种方法生长的单晶粉体的XRD分析结果显示,单晶具有立方晶系结构,其晶格常数分别为0.56833、0.56891 nm。实验测量了CH_3NH_3PbCl_3单晶的红外光... 详细信息

分别采用蒸发结晶法和逆温结晶法生长尺寸约为4 mm′3 mm′3 mm的CH_3NH_3PbCl_3单晶。对两种方法生长的单晶粉体的XRD分析结果显示,单晶具有立方晶系结构,其晶格常数分别为0.56833、0.56891 nm。实验测量了CH_3NH_3PbCl_3单晶的红外光谱(FT-IR)和拉曼光谱,并对谱峰进行了指认;使用UV-VIS-NIR分光光度计、荧光光度计对CH_3NH_3PbCl_3单晶的光学性能进行了测试。结果表明:CH_3NH_3PbCl_3晶体的吸收边约为423 nm,光致发光峰为433 nm,带隙值为2.97 e V,与CH_3NH_3PbCl_3薄膜的光学特性相比,CH_3NH_3PbCl_3单晶更具潜在的应用前景。最后,结合第一性原理研究了CH_3NH_3PbCl_3晶体的能带结构,计算得出带隙值2.428 e V,与实验值吻合较好。

关键词： CH3NH3PbCl3单晶蒸发结晶法逆温结晶法光谱

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