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Human-induced pluripotent stem cell-derived neural stem cell exosomes improve blood-brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

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Neural Regeneration Research 2025年第2期20卷 518-532页

作者： Conglin Wang Fangyuan Cheng Zhaoli Han Bo Yan Pan liao Zhenyu Yin Xintong Ge Dai li Rongrong zhong Qiang liu Fanglian Chen Ping Lei Department of Geriatrics Tianjin Medical University General HospitalTianjinChina School of Medicine Nankai UniversityTianjinChina Tianjin Neurological Institute TianjinChina

Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor ***-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system *** this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying *** results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral ***,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory *** sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier *** with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these *** summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.

关键词： AKT astrocyte blood-brain barrier cerebral edema exosomes human-induced pluripotent stem cells intracerebral hemorrhage neural stem cells neuroinflammation PI3K

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Expression of sigma receptor 1 in rat retina

Expression of sigma receptor 1 in rat retina

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Lei-Lei liU,Lu WANG,yong-Mei zhong~*,Xiong-li YANG Institute of Neurobiology,Institute of Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032,China

＜正＞Sigma receptors（aRs） are nonopiate,nonphencyclidine binding sites in mammalian brain and peripheral organs, distinct from other known *** are thought to have neuroprotective role via modulation of N-methyl-Daspartate （NMDA） *** date,two types of oRs（oRl andσR2） have been *** this work,expression ofσR1 in rat retina was studied using immunofluorescence double labeling and confocal laser scanning *** blot analysis demonstrated that the antibody was specific in rat *** transcription-polymerase chain reactive （RT-PCR） analysis showed thatσR1 mRNA was present in rat *** forσR1 was diffusely distributed throughout the full thickness of the inner plexiform ***σR1 -immunoreactivity（IR） was also observed in lots of cells in the inner nuclear layer and in the ganglion cell ***σR1-IR was detectable in the outer *** labeling experiments further showed thatσR1 was expressed in a population of glycinergic amacrine cells（ACs） as well as glycine-labeled bipolar cells（BCs） that could be cone ON type *** labeling was seen in parvalbumin-positive All *** addition,σR1-IR was also seen in almost all somata of ganglion cells retrogradely labeled by *** results suggest thatσR1 may play neuroprotective roles for neurons in the inner retina.

关键词： σR1 immunofluorescence double labeling amacrine cells ganglion cells retina

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The Citron homology domain of MAP4Ks improves outcomes of traumatic brain injury

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Neural Regeneration Research 2025年第11期20卷 3233-3244页

作者： Xiaoling zhong Wenjiao Tai Meng-Lu liu Shuaipeng Ma Tianjin Shen Yuhua Zou Chun-li Zhang Department of Molecular Biology University of Texas Southwestern Medical CenterDallasTXUSA Hamon Center for Regenerative Science and Medicine University of Texas Southwestern Medical CenterDallasTXUSA Peter O’Donnell Jr.Brain Institute University of Texas Southwestern Medical CenterDallasTXUSA

The mitogen-activated protein kinase kinase kinase kinases(MAP4Ks)signaling pathway plays a pivotal role in axonal regrowth and neuronal degeneration following *** targeting this pathway is beneficial to brain injury remains *** this study,we showed that adeno-associated virus-delivery of the Citron homology domain of MAP4Ks effectively reduces traumatic brain injury-induced reactive gliosis,tauopathy,lesion size,and behavioral *** inhibition of MAP4Ks replicated the ameliorative effects observed with expression of the Citron homology ***,the Citron homology domain acted as a dominant-negative mutant,impeding MAP4K-mediated phosphorylation of the dishevelled proteins and thereby controlling the Wnt/β-catenin *** findings implicate a therapeutic potential of targeting MAP4Ks to alleviate the detrimental effects of traumatic brain injury.

关键词： adeno-associated virus Citron homology Citron homology domain gene therapy mitogen-activated protein kinase kinase kinase kinases traumatic brain injury

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Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

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Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu Guo Lanyue Zhang Gening Du Hao You Junfeng Huang Mudong Tu Hua li yong Peng Wei Dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 mA cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： X ray photoelectron spectroscopy

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高抗冲及强表面附着力PET合金的制备与性能研究

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塑料工业 2018年第10期46卷 91-94页

作者：邓小亮周欣肖勇钟鹏李俊峰普拉斯包装材料有限公司聚酯事业部四川宜宾644007

采用聚乙烯-辛烯共聚弹性体(POE)接枝马来酸酐(POE-g-MAH)增韧聚对苯二甲酸乙二醇酯(PET),同时为了提高PET/POE-g-MAH产品的电镀性能,通过乙烯丙烯酸类聚合物(EAA)对合金进行增黏改性。冲击测试表明,POE-g-MAH能够大幅度提高PET的抗冲... 详细信息

采用聚乙烯-辛烯共聚弹性体(POE)接枝马来酸酐(POE-g-MAH)增韧聚对苯二甲酸乙二醇酯(PET),同时为了提高PET/POE-g-MAH产品的电镀性能,通过乙烯丙烯酸类聚合物(EAA)对合金进行增黏改性。冲击测试表明,POE-g-MAH能够大幅度提高PET的抗冲击性能,结合公司产品使用环境以及成本,选择添加10%POE-g-MAH用于提高PET韧性能够达到综合性能优异、成本低的要求。另外,加入增黏剂后,PET/POE-g-MAH共混体系的冲击强度先上升后下降,当EAA含量为2%时,附着力与冲击强度均较大,并且底座电镀后百格测试油漆无脱落。

关键词：聚对苯二甲酸乙二醇酯附着力电镀增韧

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人巨细胞病毒cDNA文库的构建、鉴定及pp65阳性克隆筛选

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微生物学通报 2004年第2期31卷 87-90页

作者：韩俊李艳秋姜永中余莉王明丽安徽医科大学微生物学教研室

为进一步进行人巨细胞病毒 (HCMV)后基因组功能的研究 ,以及为疫苗分子和早期诊断试剂的研制提供有效工具 ,从HCMVAD1 69株感染 96h的HF细胞中提取HCMV的mRNA ,逆转录合成cDNA片段 ,重组入λgt1 1EcoRI酶切位点之间 ,包装蛋白包装 ,构建... 详细信息

为进一步进行人巨细胞病毒 (HCMV)后基因组功能的研究 ,以及为疫苗分子和早期诊断试剂的研制提供有效工具 ,从HCMVAD1 69株感染 96h的HF细胞中提取HCMV的mRNA ,逆转录合成cDNA片段 ,重组入λgt1 1EcoRI酶切位点之间 ,包装蛋白包装 ,构建HCMVAD1 69株基因组cDNA表达文库。结果表明 ,初始HCMVcDNA文库容量为 3 6× 1 0 6,重组率为 96% ;HCMV鼠多克隆抗血清筛选出 1 68个HCMV阳性克隆 ;地高辛标记pp65特异性寡核苷酸探针原位噬斑杂交筛选出 3 4个pp65阳性克隆 ,再经PCR扩增 ,筛选出 2个pp65阳性克隆 ;pp65PCR扩增产物进一步被Southernblotting证实 ;3 端测序比较 ,同源性为 98%。为进一步克隆。

关键词：人巨细胞病毒 pp65 cDNA表达文库

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Activation of the sigma receptor 1 modulates NMDA receptors in rat retinal ganglion cells

Activation of the sigma receptor 1 modulates NMDA receptors ...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xin-Jun ZHANG,Lei-Lei liU,yong-Mei zhong,Xiong-li YANG~* Institute of Neurobiology,Institutes of Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032,China

Sigma receptors(oRs) represent unique nonopiate,nonphencyclidine binding sites in mammalian nervous *** is evidence that ligands of aRs have robust neuroprotective *** the present work,we investigated howσR1 is expressed in the rat retina and how activation ofσR1 modulates NMDA receptors in rat retinal ganglion cells (GCs).By immunocytochemistry,we showed thatσR1 was strongly expressed in the somata of amacrine cells and GCs. Labeling forσR1 was also observed in both outer and inner plexiform *** blotting demonstrated thatσR1 was predominantly distributed in the fractions of cell membrane and cytoplasm,but not in those of *** whole-cell recordings in retinal slice preparations,we first characterized NMD A receptor-mediated currents of GCs.A robust inward current was induced from GCs with focal puff of 1 mM NMD A to the dendrites or with puff of 120 mM KCl onto the somata of associated bipolar cells in the absence of *** current could be completely blocked by D-Aβ5,a specific NMDA receptor *** further showed that theσR1 agonist SKF10047 or PRE-084 suppressed NMDA-induced currents in a dose-dependent manner from both ON and OFF type GCs and the effect could be reversed by theσR1 antagonist BD1047 or ***,activation ofσR1 could also suppress NMDA receptor-mediated ON and OFF light responses of *** results suggest that theσR1-mediated neuroprotective effects on GCs may be,at least in part, due to the suppression of NMDA receptors caused by activation ofσR1.

关键词： σR1 NMD A receptor ganglion cells retina

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Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

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Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： Lu Guan Mengting Qiu Na li Zhengxiang Zhou Ru Ye liyan zhong Yashuang Xu Junhui Ren Yi liang Xiaomei Shao Jianqiao Fang Junfan Fang Junying Du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health Service Center of Fuyang District HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission

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肝泡状棘球蚴病的超声及病理表现

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世界华人消化杂志 2008年第26期16卷 3001-3004页

作者：敬基刚李永忠庄华彭玉兰四川大学华西医院超声诊断科阿坝州人民医院超声科四川省马尔康县624000

目的:分析肝泡状棘球蚴病患者的临床、病理及超声图像特征.方法:回顾性分析2002-2007年我院经手术病理证实的肝泡状棘球蚴病患者15例的超声图像特征并分型.结果:15例肝泡状棘球蚴病单发12例,多发3例,共发现肿块20个;超声特征:多位于左肝... 详细信息

目的:分析肝泡状棘球蚴病患者的临床、病理及超声图像特征.方法:回顾性分析2002-2007年我院经手术病理证实的肝泡状棘球蚴病患者15例的超声图像特征并分型.结果:15例肝泡状棘球蚴病单发12例,多发3例,共发现肿块20个;超声特征:多位于左肝,大小多为5cm以上,形态多不规则,边界多清晰,内部回声多呈高回声、不均匀、无液化、无钙化,后方回声多衰减,无声晕、无内部血流多见,未见肝门及腹腔淋巴结肿大;根据声像图特征分为:实性肿块型(13个,65%)、肿块液化型(7个,35%)、肿块钙化型(8个,40%).结论:超声检查肝泡球蚴病超声影像具有特征性,是肝泡球蚴病重要的影像学检查方法.

关键词：泡状棘球蚴病肝脏超声

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Multivariate synergy for heightening ionic HOF robustness and proton conductivity

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Science China Chemistry 2025年

作者： Xiang-Tian Bai li-Hui Cao Xu-yong Chen Jia-Yu Wang Zi-Ye Zhou Shaanxi Key Laboratory of Chemical Additives for Industry College of Chemistry and Chemical EngineeringShaanxi University of Science and Technology

Multicomponent crystalline materials construct more complex and diversified structures with different combinations and modes,which expands the material structure space and application *** this work,we have successfully synthesized two twocomponent ionic hydrogen-bonded organic frameworks （iHOF-32 and iHOF-33） and a three-component iHOF （iHOF-34）.Guanidine hydrochloride which is rich in N and H and has a short chain length strengthens the hydrogen bonding network of iHOF,thereby aiding in the efficient migration of *** rigid architecture of 1,1'-diamino-4,4'-bipyridine diiodide furnishes additional scaffolding for the iHOFs and enhances its robustness under harsh ***-34 accomplishes the combination of the ultra-high proton conductivity of iHOF-32 and the robustness of iHOF-33 by the synergistic interaction among the three components of *** addition,iHOF-34 was fabricated as a composite membrane with a peak proton conductivity of 1.55×10-1S cm-1,which is 3.8 times higher than that of recast Nafion,and has extensive potential in fuel *** self-assembly strategy of multicomponent HOFs opens up new avenues for constructing diverse structures while promising new opportunities for applications in the fields of proton conduction and energy storage.

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