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Bone marrow-derived dendritic cells pulsed with tumor lysates induce anti-tumor immunity against gastric cancer ex vivo

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World Journal of Gastroenterology 2008年第46期14卷 7127-7132页

作者： Yan-Lin Li Yu-Gang Wu yong-qing wang Zhong Li Rong-Chao wang Liang wang Yan-Yun Zhang Department of Paediatrics the First People Hospital of Changzhou and The Third Affiliated Hospital of Soochow University Changzhou 213000 Jiangsu Province China Department of Surgery the First People Hospital of Changzhou and The Third Affiliated Hospital of Soochow University Changzhou 213000 Jiangsu Province China Department of Surgery the First Affiliated Hospital of Sooehow University Suzhou 215006 Jiangsu Province China Institute of Health Sciences and Shanghai Institute of Immunology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

AIM： To investigate whether bone marrow-derived denritic cells pulsed with tumor lysates induce immunity against gastric cancer ex vivo. METHODS： c-kit＋ hematopoietic progenitor cells were magnetically isolated with a MiniMACS separator from BALB/c mice bone marrow cells. These cells were cultured with cytokines GM-CSF, IL-4, and TNFα to induce their maturation. They were analysed by morphological observation, phenotype analysis, and mixed lymphocyte reaction （MLR）. Bone marrowderived DCs （BM-DCs） were pulsed with tumor cell lysate obtained by rapid freezing and thawing at a 1：3 DC：tumor cell ratio. Finally, cytotoxic T lymphocyte （CTL） activity and interferon gamma （IFNγ） secretion was evaluated ex vivo. RESULTS： c-kit^＋ hematopoietic progenitor cells from mice bone marrow cells cultured with cytokines for 8 d showed the character of typical mature ***, observed by light microscope, these cells were large with oval or irregularly shaped nuclei and with many small dendrites. Phenotypically, FACS analysis showed that they *** levels of la, DEC-205, CD11b, CD80 and CD86 antigen, moderate levels of CD40, and negative for F4/80. Functionally, these cells gained the capacity to stimulate allogeneic T cells in MLR assay. However, immature DCs cultured with cytokines for 5 d did not have typical DCs phenotypic markers and could not stimulate allogeneic T cells. Ex vivo primed T cells with SGC-7901 tumor cell lysate-pulsed （TP） DCs were able to induce effective CTL activity against SGC-7901 tumor cells （E：T = 100：1, 69.55% ± 6.05% specific lysis）, but not B16 tumor cells, and produced higher levels of IFNγ, when stimulated with SGC-7901 tumor cells but not when stimulated with B16 tumor cells （1575.31 ± 60.25 pg/mL in SGC-7901 group vs 164.11± 18.52 pg/mL in B16 group, P 〈 0.01）. CONCLUSION： BM-derived DCs pulsed with tumor lysates Can induce anti-tumor immunity specific to gastric cancer ex vivo.

关键词： Dendritic cells Cytokine Gastric cancer Cytotoxic T lymphocyte Imrnunotherapy

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Analysis of the Characteristics of Pregnancy and Delivery before and after Implementation of the Two-child Policy

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Chinese Medical Journal 2018年第1期131卷 37-42页

作者： Hong-Xia Zhang Yang-Yu Zhao yong-qing wang Department of Obstetrics and Gynecolog Peking University Third Hospital Beijing 100191 China

Background： After the two-child policy is frilly implemented, new challenges regarding pregnancy management and the treatment of pregnancy complications will arise. The aim of this study was to analyze the characteristics of pregnancy and delivery before and after the implementation of the two-child policy to make suggestions on the quality assurance of the new era of obstetrics. Methods： In total, 5895 cases of pregnant women who delivered from April 2016 to March 2017 in Peking University Third Hospital served as the study group and 5103 cases of pregnant women who delivered from January to December 2015 served as the control group. The characteristics of pregnancy and delivery were retrospectively analyzed. Results： In the study group, the percentage of pregnant women who were older （over 40 years）（3.6% vs. 2.2%）, were multipara （30.3% vs. 17.0%）, received irregular prenatal care （ 1.5% vs. 0.9%）, were transferred for treatment from a subordinate hospital （4.4% vs. 2.8%）, and were not residents of Beijing （3.8% vs. 2.2%）, were significantly increased compared with the control group （P 〈 0.05）. In the study group, the rate of a hypertensive disorder complicating pregnancy （6.4% vs. 5.0%）, gestational diabetes mellitus （25.3% vs. 23.1%）, dangerous placenta previa （3.0% vs. 2.3%）, placental implantation （2.4% vs. 1.8%）, and severe postpartum hemorrhage （2.8% vs. 1.9%） was significantly increased compared with the control group （P 〈 0.05）. In the study group, the cesarean section rate during primipara was significantly reduced compared with the control group （42.0% vs. 44.2%）. However, the rate during inultipara was significantly increased compared with the control group （P 〈 0.05）. Indications for cesarean section in the study group as well as the percentages of scared uterus and placenta previa were significantly increased compared with the control group （P 〈 0.05）. Conclusions： According to the current situation, better methods are needed to strengthen pregnancy

关键词： Cesarean Section Rate Pregnancy Complications Two-child Policy

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Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection

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Chinese Chemical Letters 2010年第10期21卷 1231-1234页

作者： Yin Yan Zhang Zi Cheng Li Jin Kun Zhu Zhi yong Yang qing Jiang wang Pin Gang He Yu Zhi Fang Department of Chemistry East China Normal University Shanghai 200062 China

A high-performance capillary electrophoresis with amperometry detection method （CE-AD） has been developed for the analysis of flavonoids and anthraquinones （emodin, kaempferol, apigenin, luteolin and rhein） in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17 min using a borate-phosphate running buffer （1.5 ×10^-2 mol/L borate-3× 10^-2 mol/L phosphate running buffer, pH 9.0） at a working potential of ＋0.90 V （vs. SCE） and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits （S/N = 3） ranging from 1.0 × 10^-7 to 2.1 × 10^-7 g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.

关键词： Capillary electrophoresis Amperometry detection Chrysanthemu Flavonoids Anthraquinones

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Investigation of diagnosis and treatment of hemolysis-elevated liver enzymes-low platelet counts （HELLP） syndrome： clinical analysis of 59 cases

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Chinese Medical Journal 2010年第10期123卷 1273-1277页

作者： wang yong-qing wang Jing YE Rong-hua ZHAO Yang-yu Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China

Background Hemolysis-elevated liver enzymes-low platelet counts （HELLP） syndrome is a clinical condition occurring in middle and late stage pregnancy. It is characterized by hemolysis, elevated liver enzymes and low platelet counts. This study involves the analysis of the diagnosis, clinical characteristics and treatment of 59 cases of HELLP syndrome as well as the clinical classification, method of delivery and gestational age at delivery. Methods Clinical data from 59 cases of HELLP syndrome occurring from January 2000 to December 2009 were analyzed retrospectively. Thirty-five cases were classified as complete HELLP syndrome and 24 cases were considered partial HELLP syndrome. Results Twenty-six of the 59 analyzed patients （44%） with complete HELLP syndrome showed rapid onset, severe signs, symptoms, and complications in addition to a poor clinical outcome. Complications included multiple organ dysfunction syndrome （MODS） occurring in 18 cases, eclampsia （3 cases）, placental abruption （3 cases）, and perinatal death （4 cases）. The remaining 33 cases （24 with partial and 9 with complete HELLP） were characterized by less severe signs, symptoms, complications and progression of the condition. Two of these cases were complicated with MODS （6.1%）, and 1 with perinatal death （3.0%）.Twelve non-radical-type cases received conservative treatment. The remaining 4 patients had recurring HELLP syndrome （6.78%）. Conclusions HELLP syndrome is classified as the radical type and non-radical-type according to clinical characteristics and outcome. Classification of HELLP syndrome cases according to clinical features can help in the monitoring and treatment of the disease. Active termination of pregnancy should be considered for radical-type cases. Non-radical-type cases can undergo conservative treatment with close monitoring in an attempt to improve perinatal outcome without increasing maternal morbidity.

关键词： HELLP syndrome severe preeclampsia diagnosis treatment

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Correlation between intracoronary thrombus components and coronary blood flow after percutaneous coronary intervention for acute myocardial infarction at different onset time

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World Journal of Clinical Cases 2019年第15期7卷 2013-2021页

作者： Ming-Ji Zhang Xin Liu Li-Hong Liu Ning Li Ning Zhang yong-qing wang Xue-Jun Sun Ping-He Huang Hong-Mei Yin yong-Hui Liu Hong Zheng Department of Cardiology AnSteel Group Hospital

BACKGROUND Acute myocardial infarction(AMI)is a leading cause of *** reperfusion to restore blood flow is crucial to successful *** the current reperfusion regimen,an increasing number of patients have benefited from direct percutaneous coronary intervention(PCI).In order to understand whether there is a correlation between the components of coronary thrombosis and the absence of reflow or slow blood flow after coronary stent implantation in direct PCI,we collected data on direct PCI cases in our hospital between January 2016 and November *** To investigate the correlation between intracoronary thrombus components and coronary blood flow after stent implantation in direct PCI in *** We enrolled 154 patients(85 male and 69 female,aged 36–81 years)with direct PCI who underwent thrombus catheter aspiration within<3,3–6 or 6–12 h of onset of AMI between January 2016 and November *** thrombus was removed for pathological examination under a *** patients of the three groups according to the onset time of AMI were further divided into those with a white or red *** thrombolysis in myocardial infarction(TIMI)blood flow after stent implantation was recorded based on digital subtraction angiography during *** number of patients with no-reflow and slow blood flow in each group was *** analysis was performed based on data such as onset time,TIMI blood *** There were significant differences in thrombus components between the patients with acute ST-segment elevation myocardial infarction and non-ST-segment elevation myocardial infarction(P<0.01).In the group with PCI<3 h after onset of AMI,there was no significant difference in the incidence of no-reflow and slow-flow between the white and red thrombus *** the groups with PCI 3-6 and 6-12 h after onset of AMI,there was a significant difference in the incidence of no-reflow and slow-flow between the white and red thrombus groups(P<0.01).Th

关键词： Acute myocardial infarction Pathological thrombotic component Direct percutaneous coronary intervention Blood flow

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Dynamic-mechanical analysis and SEM morphology of wood flour/polypropylene composites

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Journal of Forestry Research 2006年第4期17卷 315-318页

作者： GUO Chui-gen SONG yong-ming wang qing-wen SHEN Chang-sheng MOE Key Laboratory of Bio-based Material Science and Technology Northeast Forestry University Harbin 150040 P. R. China

A study was conducted to investigate the effects of compatibilizers, including Maleic anhydride grafted polypropylene （MA-PP） and maleic anhydride grafted ethylene-propylene-diene copolymer （MA-EPDM）, on wood-flour/polypropylene （WF/PP） composites. WF/PP composites were prepared by direct extrusion profiles using a twin-screw/single-screw extruder system. DMA analysis showed that the loss factor of composites decreased and the storage modulus improved in the presence of MA-PP, which indicated much better interfacial adhesion between the PP matrix and wood flour filler than in the absence of compatibilizer. Morphological feature based on SEM observation showed that MA-PP and MA-EPDM improved the dispersion of the wood particles in the plastic matrix. MA-EPDM is a soft segment, although it improved the interfacial adhesion, storage modulus decreases with adding of MA-EPDM. As compatibilizer of wood-flour/polypropylene composites, both DMA analysis and SEM feature proved that MA-PP was superior to MA-EPDM.

关键词： Wood flour Polypropylene Composites Dynamic-mechanical analysis

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Dermatofibrosarcoma protuberans of the chest wall： three-dimensional wide excision and reconstruction

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Chinese Medical Journal 2014年第2期127卷 386-388页

作者： Zhang Zhenyu Cen Ying qing yong wang Huaisheng Liu Xiaoxue Department of Plastic and Burn Surgery West China Hospital Sichuan University Chengdu Sichuan 610041 China

Dermatofibrosarcoma protuberans （DFSP） is a poorly differentiated interstitial tumor that originates from the dermis. It occurs predominantly in the 20-50-year- old males. DFSP generally presents as local pink plaques or nodules and subsequently develops into nodular masses accompanied by infiltrative growth to the surrounding tissue, including muscle and bone.

关键词： dermatofibrosarcoma protuberans surgery chest wall reconstruction

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A case of aggravation of hemolytic anemia, elevated liver enzymes and low platelet count syndrome after delivery

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Chinese Medical Journal 2011年第8期124卷 1261-1263页

作者： JIANG Yuan-hui wang yong-qing wang Jing YE Rong-hua Department of Obstetrics and Gynecology Peking UniversityThird Hospital Beijing 100191 China

Background Hemolytic anemia, elevated liver enzymes and low platelet count （HELLP） syndrome is a severe obstetric complication which usually resolves in most patients after *** We report a rare case of aggravation of HELLP syndrome after *** The patient underwent the treatment for HELLP syndrome,.including glucocorticoid therapy. The symptoms of HELLP syndrome reappeared and became more severe than before the termination of pregnancy. The patient also had severe and persistent hypoproteinemia, hyponatremia and *** HELLP syndrome is an acute and critical obstetric syndrome which can have heterogeneous presentations and variable prognosis. We should be fully aware of the diverse clinical characteristics of this condition.

关键词： HELLP syndrome hypocalcemia hyponatremia hypoproteinemia

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Efficacy of Different Treatment Regimens for Antiphospholipid Syndrome-related Recurrent Spontaneous Abortion

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Chinese Medical Journal 2017年第12期130卷 1395-1399页

作者： Sheng-Long Ye Xun-Ke Gu Li-Yuan Tao Ji-Mei Cong yong-qing wang Department of Obstetrics and Gynaecology Peking University Third Hospital Beijing 100191 China Research Center of Clinical Epiderniology Peking University Third Hospital Beijing 100191 China

Background： Antiphospholipid syndrome （APS）-related immune factors are considered as an important cause of recurrent spontaneous abortion （RSA）. Anticoagulant and anti-inflammatory treatments are believed to effectively improve adverse pregnancy outcomes by affecting the abnormal autoimmune response of the maternal-fetal interface. The aim of this study was to observe the clinical characteristics and treatment outcomes of anticoagulant regimens and anti-inflammatory plus anticoagulation regimens for APS-related RSA. Methods： APS-related RSA cases from September 2011 to September 2016 at Peking University Third Hospital were retrospectively analyzed. The patients were assigned to study group （anti-inflammation plus anticoagulation） and control group （simple anticoagulation）, The incidence of repeat abortion, the incidence of placental dysfunction, the gestational weeks of pregnancy, and the mean weight of the fetus were observed. Results： The pregnancy and neonatal outcome indicators of the repeat pregnancy loss rate （ 11. 11% vs. 22.70%）, placental dysfunction-related diseases （6.35% vs. 15.60%）, the mean birth weight of infants born after 24 weeks gestation （3152.4 ± 844.67 g vs. 2765.76 ± 816.40 g）, full-ternl delivery weight （3456.28 ±419.79 g vs. 3076.18±518.79 g）, the proportions of low birth weight infants （ 12.70% vs. 21.98%）, and small for gestational age （6.35% vs. 14.18%） differed significantly between the study and control groups （all P 〈 0.05）. The incidence of preterm delivery, term delivery, and stillbirth was not significantly different between the two groups, and there was no significant difference between the study and control groups in gestational age at birth （37.6 ± 3.3 weeks vs. 36.9 ± 3.2 weeks： P 〉 0.05）. Conclusion： The anti-inflammatory and anticoagulation regimen is more effective than the simple anticoagulation regimen in the treatlnent of APS recurrent abortion.

关键词： Anticoagulant Therapy Anti-inflammatory Therapy Antiphospholipid Syndrome Recurrent Spontaneous Abortion

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Experimental study on mechanism and protection of stress ulcer produced by explosive noise

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World Journal of Gastroenterology 1998年第6期4卷 64-68页

作者： LIU Guo Shi 1, HUANG Yu Xin 1, LI Shuan Wei 2, PAN Bo Rong 3, wang Xin 1, SUN Da yong 1 and wang qing Li 1

AIM To establish an experimental model of stress ulcer produced by explosive noise, and to probe into its mechanism and protection. METHODS The country standard Wistar white rats were randomly divided into control group ( n =8), which were neither stimulated nor protected, and stimulating group (divided into subgroups A, B and C, including 8 rats each which were decapitated to draw blood for test immediately, 12 hours and 24 hours after stimulation) and prevention group (divided into subgroups A, B and C, having 8 rats each, subgroup A was given cimetidine, B anisodamine and C both drugs). Firing noises of submachine guns were used as inflicting factor. The rats were fasted for 24 hours and stimulated by firing noise for 12 hours. The change of ulcer index, gastric mucosal and related serum hormones were observed. RESULTS Stress ulcer was significant in the stimulating group, and its ulcer index (8 6±0 6) was remarkably higher than that in both the control group and prevention group (0 3±0 1, P <0 01). Its serum gastrin (Gas ng/L , 294±163 vs 63±40, P <0 01) and endothelin (ET ng/L , 181±57 vs 135±42, P <0 01) were apparently higher than those in the control group, and its serum nitric oxide (NO) level was conspicuously lower than that in the control group ( ng/L , 0 2±0 1 vs 0 8±0 5, P <0 05), while the serum gastrin level ( ng/L , 556±225) in prevention group was distinctly higher than that in both the control ( P <0 01) and stimulating group ( P <0 05). There were no significant differences in the changes of ET and NO between the control and the stimulating groups. CONCLUSION Stress ulcer model of rats can be successfully established by the stimulation of explosive noise. Gas, ET and NO are related to the formation of stress ulcer, and play an important role in its mechanism. Hepatic function affected by noise is observed in this experiment.

关键词： stomach ulcer/etiology stomach ulcer/prevention and control gastric mucosa/pathology noise/adverse effects stress ulcer

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