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Aberrant activation of latent transforming growth factor-β initiates the onset of temporomandibular joint osteoarthritis

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Bone Research 2018年第4期6卷 383-392页

作者： Liwei Zheng Caixia Pi jun Zhang Yi Fan Chen Cui Yang Zhou Jianxun Sun Quan Yuan Xin Xu Ling ye Xu Cao Xuedong Zhou State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University Department of Orthopaedic Surgery School of Medicine Johns Hopkins University

There is currently no effective medical treatment for temporomandibular joint osteoarthritis(TMJ-OA) due to a limited understanding of its pathogenesis. This study was undertaken to investigate the key role of transforming growth factor-β(TGF-β)signalling in the cartilage and subchondral bone of the TMJ using a temporomandibular joint disorder(TMD) rat model, an ageing mouse model and a Camurati–Engelmann disease(CED) mouse model. In the three animal models, the subchondral bone phenotypes in the mandibular condyles were evaluated by μCT, and changes in TMJ condyles were examined by TRAP staining and immunohistochemical analysis of Osterix and p-Smad2/3. Condyle degradation was confirmed by Safranin O staining, the Mankin and OARSI scoring systems and type X collagen(Col X), p-Smad2/3 a and Osterix immunohistochemical analyses. We found apparent histological phenotypes of TMJ-OA in the TMD, ageing and CED animal models, with abnormal activation of TGF-βsignalling in the condylar cartilage and subchondral bone. Moreover, inhibition of TGF-β receptor I attenuated TMJ-OA progression in the TMD models. Therefore, aberrant activation of TGF-β signalling could be a key player in TMJ-OA development.

关键词： TMJ OA TMD Aberrant activation of latent transforming growth factor initiates the onset of temporomandibular joint osteoarthritis

温度对厚壳贻贝(Mytilus coruscus)鳃组织纤毛结构及摄食率的影响机制研究

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海洋与湖沼 2024年第2期55卷 471-478页

作者：梁泽玮李伟业殷小龙徐羡孙闻婧王雪儿陈传悦廖智严小军张晓林浙江海洋大学海洋科学与技术学院浙江舟山316022 浙江省舟山市水产研究所浙江舟山316000

纤毛是着生在贻贝鳃上感知外界物理和化学信号并帮助食物转运的一类重要细胞结构,温度的变化会改变纤毛的形态和生理生化特征,从而影响贻贝的摄食行为。为探究不同温度下厚壳贻贝的摄食率变化,以及温度对纤毛的形态、酶活和相关基因表... 详细信息

纤毛是着生在贻贝鳃上感知外界物理和化学信号并帮助食物转运的一类重要细胞结构,温度的变化会改变纤毛的形态和生理生化特征,从而影响贻贝的摄食行为。为探究不同温度下厚壳贻贝的摄食率变化,以及温度对纤毛的形态、酶活和相关基因表达的影响,实验设置16、26和32℃三种温度处理厚壳贻贝,处理24h后测定1h内每个处理组的摄食率,同时采用组织学方法对各处理组鳃上的纤毛形态结构进行观察,并测定鳃组织的抗氧化酶活力和纤毛相关基因tekt1、tekt2、tekt3、tekt4、caf58、caf100、dnah、rsph99和ift74的表达变化。实验结果显示,32℃处理组厚壳贻贝的摄食率显著低于其他两组(P<0.05),且32℃处理组贻贝鳃上的前纤毛、侧纤毛以及纤毛细胞受损严重并部分脱落,血腔间隙破损暴露,而其余两组鳃上纤毛形态较为正常;不同温度处理下鳃组织的过氧化氢(H2O2)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力呈现温度特异性;与16℃处理组相比,26℃处理组中除tekt4和ndah外其他7个纤毛相关基因表达均显著上调,32℃处理组除tekt1外其余8个基因表达均显著下调(P<0.05)。上述结果表明,32℃高温处理对贻贝鳃上纤毛造成严重损伤,导致纤毛运动、纤毛再生和纤毛感知功能均受到一定程度的抑制,从而影响食物颗粒的运输,造成摄食率的降低。

关键词：厚壳贻贝温度纤毛摄食率细胞氧化损伤

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温度和盐度对香港牡蛎碳收支的影响

南方农业学报 2024年第8期55卷 2504-2513页

作者：官俊良何庆松张兴志何苹萍韦嫔媛张立陈泳先白科许尤厚彭金霞广西水产科学研究院/农业农村部中国(广西)-东盟水产种业资源开发与综合利用重点实验室(省部共建)/广西水产遗传育种与健康养殖重点实验室广西南宁530021 广西北部湾海洋生物多样性养护重点实验室/北部湾海洋生态环境广西野外科学观测研究站/北部湾大学海洋学院广西钦州535011

【目的】明确温度和盐度对香港牡蛎生理代谢及碳收支的影响,为解析香港牡蛎碳收支分配机制提供参考依据,也为后续探究牡蛎固碳功能及发展蓝色、低碳的新兴经济产业奠定理论基础。【方法】利用静水法测定香港牡蛎在不同温度(12、19、26... 详细信息

【目的】明确温度和盐度对香港牡蛎生理代谢及碳收支的影响,为解析香港牡蛎碳收支分配机制提供参考依据,也为后续探究牡蛎固碳功能及发展蓝色、低碳的新兴经济产业奠定理论基础。【方法】利用静水法测定香港牡蛎在不同温度(12、19、26和33℃)和盐度(6‰、15‰、24‰和33‰)条件下的耗氧率、排氨率、摄食率和排粪率,并建立香港牡蛎的碳收支方程。【结果】香港牡蛎耗氧率随温度的升高而逐渐升高,在试验设定的最高温度(33℃)时达最大值[1.87 mg/(g∙h)];排氨率随温度的升高呈先下降后上升趋势,根据拟合方程计算得知在17.70℃下的排氨率达最小值[0.02 mg/(g∙h)];摄食率随温度的升高呈先上升后下降的变化趋势,根据拟合方程计算得知在21.99℃下的摄食率达最大值[17.54 mg/(g∙h)];排粪率随温度的变化无明显规律。不同盐度条件下,香港牡蛎摄食率和耗氧率随盐度的增加呈先上升后下降趋势,根据拟合方程计算得知,盐度为21.54‰时的耗氧率达最大值[1.29 mg/(g∙h)],盐度为18.88‰时的摄食率达最大值[28.48 mg/(g∙h)];排氨率和排粪率随盐度的增加整体上呈下降趋势,二者在试验设定的最高盐度(33‰)下达最小值。温度和盐度对香港牡蛎的碳收支存在明显影响,在温度19~26℃、盐度15‰~24‰的条件下香港牡蛎将摄食的大部分碳用于生长。不同温度和盐度条件下,香港牡蛎的碳收支均表现为生长碳占摄食碳的比例最高,其次是粪便碳和呼吸碳,排泄碳的占比最低。【结论】温度和盐度对香港牡蛎生理代谢及碳收支均存在影响,尤其在温度19~26℃、盐度15‰~24‰的条件下生长碳占摄食碳的比例较高,更适宜其生长,具有较高的固碳能力。

关键词：香港牡蛎温度盐度生理代谢碳收支

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达格列净对2型糖尿病病人肝脏脂肪含量及胱抑素C的影响

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蚌埠医学院学报 2024年第2期49卷 225-229页

作者：汪运生朱妍夏金翔连虎叶军曹永红章容戴武安徽省合肥市第二人民医院安徽医科大学附属合肥医院内分泌科230011 安徽省合肥市第二人民医院安徽医科大学附属合肥医院超声医学科230011 安徽省合肥市第二人民医院安徽医科大学附属合肥医院磁共振影像科230011

目的:比较使用达格列净+二甲双胍联合用药与单纯使用二甲双胍治疗对2型糖尿病病人肝脏脂肪含量(LFC)和胱抑素C(Cys C)水平的影响。方法:选取住院病人121例,按照治疗方案不同分为二甲双胍治疗组(甲组,n=51)和二甲双胍联合达格列净治疗组... 详细信息

目的:比较使用达格列净+二甲双胍联合用药与单纯使用二甲双胍治疗对2型糖尿病病人肝脏脂肪含量(LFC)和胱抑素C(Cys C)水平的影响。方法:选取住院病人121例,按照治疗方案不同分为二甲双胍治疗组(甲组,n=51)和二甲双胍联合达格列净治疗组(乙组,n=70),所有病人均连续稳定使用上述治疗方案达半年以上,利用氢质子磁共振波谱法检测LFC,血清Cys C水平使用免疫比浊法进行检测,收集病人的一般人口学资料及实验室检测指标数据。分析不同实验室指标与LFC的关联,探讨不同治疗方式及其他实验指标对LFC和Cys C的影响。结果:2组病人的年龄、性别构成比、患病时长和体质量指数(BMI)的差异均无统计学意义(P>0.05);与甲组相比,乙组病人的LFC、血清Cys C水平、总胆固醇(TC)均明显降低(P<0.05~P<0.01),但三酰甘油(TG)和极低密度脂蛋白(VLDL)均升高(P<0.05);甲组的非酒精性脂肪肝(90.2%)比例高于乙组(30.0%)(P<0.01);相关分析结果发现,BMI、空腹血糖(FBG)和Cys C均与LFC存在关联(P<0.05~P<0.01)。以LFC为因变量,BMI、FBG、Cys C、病程、TC、TG和药物治疗作为自变量进行多元线性回归分析,发现与甲组相比,乙组可降低0.440个单位的LFC(P<0.01);同时以Cys C为因变量,BMI、FBG、LFC、病程、TC、TG和药物治疗作为自变量进行多元线性回归分析,与甲组相比,乙组联合用药能够降低0.689个单位的血清Cys C(P<0.01)。结论:二甲双胍联合达格列净能有效降低2型糖尿病病人LFC、血清Cys C水平,是LFC、血清Cys C的重要影响因素。

关键词： 2型糖尿病达格列净胱抑素C 肝脏脂肪含量

Stability of high-salinity-enhanced foam:Surface behavior and thin-film drainage

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Petroleum Science 2023年第4期20卷 2343-2353页

作者： Lin Sun Xue-Hui Sun yong-Chang Zhang jun Xin Hong-Ying Sun Yi-Bo Li Wan-Fen Pu Jin-Yu Tang Bing Wei National Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu610500SichuanChina No.9 Oil Production Plant PetroChina Changqing Oilfield CompanyYinchuan750006GansuChina Research Institute of Geological Exploration and Development CNPC Chuanqing Drilling Engineering Company LimitedChengdu610501SichuanChina Department of Chemical and Petroleum Engineering United Arab Emirates UniversityAl AinAbu Dhabi15551United Arab Emirates

Cocamidopropyl hydroxyl sulfobetaine(CHSB)is one of the most promising foaming agents for high-salinity reservoirs because the salt in place facilitates its foam stability,even with salinity as high as 2×10^(5)mg/***,the synergistic effects between CHSB and salt have not been fully *** study utilized bulk foam tests and thin-film interferometry to comprehensively investigate the macroscopic and microscopic decay processes of CHSB foams with NaCl concentrations ranging from 2.3×10^(4)to 2.1×10^(5)mg/*** focused on the dilatational viscoelasticity and dynamic thin-film thickness to elucidate the high-salinity-enhanced foam *** increase in dilatational viscoelasticity and supramolecular oscillating structural force(Π_(OS))with salinity dominated the superior stability of CHSB *** increasing salinity,more CHSB molecules accumulated on the surface with a lower diffusion rate,leading to high dilatational moduli and surface elasticity,thus decelerating coarsening and ***,the number density of micelles in the thin film increased with salinity,resulting in increasedΠ***,the energy barrier for stepwise thinning intensified,and the thin-film drainage *** work conduces to understand the mechanisms behind the pronounced stability of betaine foam and can promote the widespread application of foam in harsh reservoirs.

关键词： High-salinity reservoirs Betaine foam Foam stability Dilatational viscoelasticity Disjoining pressure Thin-film interferometry

Computed Tomographic Qualitative Diagnosis of Renal Masses

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The Chinese-German Journal of Clinical Oncology 2005年第6期4卷 369-372页

作者：叶慧胡道予夏军武汉华中科技大学同济医学院附属同济医院放射科 430030

Objective： To analyze the CT findings of renal masses and to investigate the value of CT imaging in qualitative diagnosis of these diseases. Methods： Twenty-eight cases of renal masses, proven surgically and pathologically, were retrospectively analyzed. Pre- and post-contrast CT scans were performed in all cases. Results： Twenty-one of 28 cases （75%） were diagnosed as having malignant tumors with 16 cases of renal cell carcinoma （RCC） showing as irregular-shaped low density soft tissue mass with no or slight enhancement. Ten of 21 cases were diagnosed as having carcinoma of the renal pelvis showing as soft tissue mass in renal pelvis with no or slight enhancement. Three of 21 cases were diagnosed as having nephroblastoma （Wilms＇ tumor） showing as larger irregular lobucated mass with no enhancement. Three of 28 cases （11%） of benign tumors were diagnosed as having angiomyolipoma （AML）. Two cases showed a mixed density mass while another case showed iso-density mass. The soft tissue part of the tumor showed enhancement while the area of fat didn＇t show any enhancement of the contrast medium. Four of 28 （14%） included 3 cases of renal cysts showing as classic cystic lesions with clear-margin. No enhancement was found. In the remaining one case of renal abscess, CT showed a mixed density mass of soft tissue and cyst with slight enhancement of soft tissue part while no enhancement of cystic part. Conclusion： Pre-contrast and post-contrast CT scans in combination with history play an important role in the diagnosis of renal

关键词： renal mass computed tomography

Comprehensive next-generation sequencing reveals double primary colorectal carcinoma missed by diagnostic imaging: A case report

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World Journal of Gastrointestinal Oncology 2023年第10期15卷 1823-1828页

作者： Yan-jun Qu Qian-Shi Zhang Bo Wang Feng Zhang Evenki Pan Chun-Yan Zhao Si-ye Liu Li-Ping Fang Department of Medical Oncology The Second Hospital of Dalian Medical UniversityDalian 116023Liaoning ProvinceChina Department of Gastrointestinal Surgery The Second Hospital of Dalian Medical UniversityDalian 116023Liaoning ProvinceChina Department of Medical Nanjing Geneseeq Technology Inc.Nanjing 211500Jiangsu ProvinceChina

BACKGROUND Multiple primary colorectal carcinoma(MPCC)is a rare clinical disease,which is challenging to differentiate from metastatic disease using histopathological ***-generation sequencing(NGS)has been employed to identify multiple primary *** SUMMARY This study a rare case of a 63-year-old male patient diagnosed with MPCC by targeted NGS,which was initially missed by radiological *** patient was found to have two tumors located on the surface of the colorectum which had distinct genomic *** on wild-type KRAS detected in the unresected tumor,the patient benefited from the epidermal growth factor receptor(EGFR)inhibitor cetuximab treatment,but developed novel mutations including KIF5B-RET fusion,which provides a possible resistance mechanism to anti-EGFR *** Our case highlights the necessity of using genetic testing for primary tumor diagnosis and the application of serial plasma circulating tumor DNA profiling for dynamic disease monitoring.

关键词： Multiple primary colorectal carcinoma Next-generation sequencing Cetuximab RET fusion Case report

胃癌合并Hp感染外周血单个核细胞中miR-22、NLRP3炎症小体表达及对预后的影响

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中华医院感染学杂志 2024年第14期34卷 2173-2177页

作者：陈伟克胡业晓朱仁武温州市中西医结合医院肿瘤科浙江温州325000 温州市中西医结合医院普外科浙江温州325000

目的探讨胃癌合并幽门螺杆菌(Hp)感染患者外周血单个核细胞(PBMCs)中微小RNA-22(miR-22)和NOD样受体蛋白3(NLRP3)炎症小体表达及对预后的影响.方法选取2019年6月-2022年6月温州市中西医结合医院收治的86例合并Hp感染胃癌患者为Hp阳性... 详细信息

目的探讨胃癌合并幽门螺杆菌(Hp)感染患者外周血单个核细胞(PBMCs)中微小RNA-22(miR-22)和NOD样受体蛋白3(NLRP3)炎症小体表达及对预后的影响.方法选取2019年6月-2022年6月温州市中西医结合医院收治的86例合并Hp感染胃癌患者为Hp阳性组,另选取同期63例无Hp感染胃癌患者为Hp阴性组,检测所有患者PBMCs中miR-22、NLRP3、凋亡相关斑点蛋白(ASC)和半胱氨酸天冬氨酸蛋白酶1(Caspase-1)水平.对Hp阳性组进行一年随访,根据患者生存情况分为生存组(n=72)、死亡组(n=14),收集对比两组患者临床资料、miR-22、NLRP3炎症小体表达,采用Kaplan-Meier法分析miR-22、NLRP3炎症小体表达对胃癌合并Hp感染患者预后的影响.结果与Hp阴性组对比,Hp阳性组PBMCs中miR-22表达水平更低,而NL-RP3、ASC和Caspase-1表达水平更高(P<0.05).生存组和死亡组患者浸润深度、淋巴结转移、临床分期、miR-22、NLRP3、ASC和Caspase-1对比,差异有统计学意义(P<0.05).Kaplan-Meier法分析显示,miR-22低表达、NLRP3高表达的胃癌合并Hp感染患者1年生存时间均低于miR-22高表达及NLRP3低表达患者(P<0.05).结论胃癌合并Hp感染患者PBMCs中miR-22、NLRP3炎症小体异常表达,且与胃癌合并Hp感染患者预后密切相关,可作为评估该类患者的预后重要指标.

关键词：胃癌幽门螺杆菌感染外周血单个核细胞微小RNA-22 NOD样受体蛋白3炎症小体预后

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Synchrotron radiation-based materials characterization techniques shed light on molten salt reactor alloys

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Nuclear Science and Techniques 2020年第1期31卷 57-71页

作者： Li Jiang Xiang-Xi ye De-jun Wang Zhi-jun Li Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Ministry of Ecology and Environment Beijing 100135China

From a safety point of view, it is important to study the damages and reliability of molten salt reactor structural alloy materials, which are subjected to extreme environments due to neutron irradiation, molten salt corrosion, fission product attacks, thermal stress, and even combinations of these. In the past few years, synchrotron radiation-based materials characterization techniques have proven to be effective in revealing the microstructural evolution and failure mechanisms of the alloys under surrogating operation conditions. Here, we review the recent progress in the investigations of molten salt corrosion,tellurium(Te) corrosion, and alloy design. The valence states and distribution of chromium(Cr) atoms, and the diffusion and local atomic structure of Te atoms near the surface of corroded alloys have been investigated using synchrotron radiation techniques, which considerably deepen the understandings on the molten salt and Te corrosion behaviors. Furthermore, the structure and size distribution of the second phases in the alloys have been obtained, which are helpful for the future development of new alloy materials.

关键词： Molten salt reactor Alloy materials Synchrotron radiation Shanghai Synchrotron Radiation Facility Molten salt corrosion Tellurium corrosion