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Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium

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World Journal of Gastroenterology 2003年第7期9卷 1554-1558页

作者： Xu-Yi Ren yong Zhou jian-peng Zhang Wei-Hua Feng Bing-Hua Jiao, Department of Biochemistry and Molecular Biology, Second Militarv Medical University, Shanghai 200433, China Department of Biochemistry and Molecular Biology Second Military Medical University Shanghai 200433 China. renxuyi2003@***

AIM: Rodent testes are generally more susceptible to cadmium (Cd)-induced toxicity than liver. To clarify the molecular mechanism of Cd-induced toxicity in testes, we compared metallothionein (MT) gene expression, MT protein accumulation, and Cd retention at different time in freshly isolated testicular interstitial cells and liver of rats treated with ***: Adult male Sprague-Dawley rats weighing 250-280 g received a s.c injection of 4.0 μmol Cd/kg and were euthanized by CO2 asphyxiation 1h, 3 h, 6 h, or 24 h *** was sampled and testicular interstitial cells were isolated. There were three replicates per treatment and 3animals per replicate for RNA analyses, others, three replicates per treatment and one animal per replicate. MT1 and MT2 mRNA levels were determined by semi-quantitative RT-PCR analysis followed by densitometry scanning, and MT was estimated by the enzyme-linked immunosorbent assay (ELISA) method. Cadmium content was determined by atomic absorption spectrophotometry. The same parametersd were also analyzed in the liver, since this tissue unquestionably accumulate ***: The rat testis expressed MT1 and MT2, the major isoforms. We also found that untreated animals contained relatively high basal levels of both isoform mRNA, which were increased after Cd treatment in liver and peaked at 3 h, followed by a decline. In contrast, the mRNA levels in interstitial cells peaked at 6 h. Interestingly, the induction of MT1 mRNA was lower than MT2 mRNA in liver of rat treated with Cd, but it was opposite to interstitial cells. Cd exposure substantially increased hepatic MT (3.9-fold increase), but did not increase MT translation in interstitial cells. CONCLUSION: Cd-induced expression of MT isoforms is not only tissue dependent but also time-dependent. The inability to induce the metal-detoxicating MT-protein in response to Cd, may account for a higher susceptibility of testes to Cd toxicity and carcinogenesis compared to liver.

关键词：金属结合蛋白基因表达镉肝毒性酶链免疫吸附实验

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Integrinα6 Targeted Near Infrared Fluorescent Imaging and Photoacoustic Imaging of Hepatocellular Carcinoma in Mice

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Journal of Clinical and Translational Hepatology 2023年第1期11卷 110-117页

作者： Yan-Zhu Lin You Wu De-Hai Cao yong-jian peng Jun Deng Wen-Jie Lin Min-Yi Si-Tu Ling Zhuo Jie-Min Chen Man-Xia Lei Rong-Bin Liu Wei-Guang Zhang jian-Jun Li Xiao-Chun Yang Guo-Kai Feng State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhouGuangdongChina Guangdong Institute for Drug Control Department of Biologic ProductsGuangzhouGuangdongChina Department of Endocrinology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongChina Department of Ultrasound Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouGuangdongChina

Background and Aims:Hepatocellular carcinoma(HCC)is the fourth most common cause of cancer-related death and ranks sixth in terms of incident cases *** purpose of this study was to develop an effective and sensitive method to distinguish liver cancer tissues from normal tissues in HCC ***α6 is a promising cell surface target for molecular imaging of HCC,where it is overexpressed and is a prognostic *** previously identified an integrinα6-targeted peptide CRWYDENAC(RWY)that has been used for positron emission tomography(PET)imaging of HCC in mouse ***:We labeled the integrinα6-targeted RWY peptide with cyanine 7(Cy7)to form an optical probe(Cy7-RWY)for near infrared fluorescent(NIRF)and photoacoustic(PA)imaging in *** transplanted with subcutaneous HCC-LM3 or orthotopic HCC-H22 cells that overexpressed integrinα6 were intravenously injected with Cy7-RWY and its corresponding *** and PA images of mice were collected from 0 to 48 h after ***:Both NIRF and PA signals started to accumulate in the tumor 2 h after injection of Cy7-RWY and peaked at 24 ***:Cy7-RWY is a promising optical probe for NIRF and PA imaging of HCC in mice,and has potential clinical application for HCC detection.

关键词： Photoacoustic imaging Near-infrared fluorescent imaging Integrinα6 Peptide Molecular imaging Hepatocellular carcinoma

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婴幼儿细菌性肺炎合并腹泻治疗方法及对免疫细胞的影响

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世界华人消化杂志 2016年第31期24卷 4311-4314页

作者：彭军彭永剑温岭市第四人民医院儿科浙江省温岭市317511 温岭市第四人民医院消化内科浙江省温岭市317511

目的观察婴幼儿细菌性肺炎合并腹泻治疗方法及对免疫细胞的影响,为临床提供依据.方法选取2015-01/2016-07诊治的婴幼儿细菌性肺炎合并腹泻患儿60例,根据治疗方案不同分为对照组(n=30)和观察组(n=30).对照组采用乳酸菌片治疗,观察组联合... 详细信息

目的观察婴幼儿细菌性肺炎合并腹泻治疗方法及对免疫细胞的影响,为临床提供依据.方法选取2015-01/2016-07诊治的婴幼儿细菌性肺炎合并腹泻患儿60例,根据治疗方案不同分为对照组(n=30)和观察组(n=30).对照组采用乳酸菌片治疗,观察组联合思密达治疗,采用流式细胞仪检测免疫指标,比较2组临床疗效及对细胞免疫的影响.结果观察组治疗7 d疗效率显著高于对照组(93.33%v s 70.00%,P<0.05);观察组大便次数正常、大便性状正常、实验室指标正常及临床症状消失时间,显著短于对照组(P<0.05);观察组治疗后细胞免疫功能水平Ig M、Ig G及Ig A,显著高于对照组(P<0.05).结论婴幼儿细菌性肺炎合并腹泻患儿在乳酸菌片基础上联合思密达治疗效果理想,能提高患儿T淋巴细胞亚群水平,值得推广应用.

关键词：婴幼儿细菌性肺炎腹泻治疗方法免疫细胞流式细胞仪

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Improved two-cuff technique for orthotopic liver transplantation in rat

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Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 33-37页

作者： yong peng, jian-Ping Gong, Lu-Nan Yan, Shou-Bo Li and Xu-Hong Li Chongqing, China Department of Hepatobiliary Surgery, Second Affilia- ted Hospital, Chongqing Medical University, Chongqing 400010, China Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041 , China Department of Hepatobiliary Surgery Second Affiliated Hospital Chongqing Medical University Chongqing 400010 China. pengyong725@***

BACKGROUND: The first orthotopic liver transplantatio in rat (ROLT) was reported by Lee in 1973. Kamada inno vatively applied cuff technique to ROLT in 1979. However the operative procedures were highly demanding and th operative mortality was relatively high. The purpose of thi study was to improve the model of ROLT, simplify opera tive procedures, and enhance the successful rate of opera tion. METHODS: Orthotopic liver transplantation was per formed in 160 Wistar rats by improved two-cuff technique The portal vein between donor and recipient was anasto mosed with the cuff technique. The same method was use to anastomose the infrahepatic vena cava. The suprahepati vena cava and the hepatic artery were anastomosed by m crovascular suturing and the bile duct was anastomosed en to end by a Teflon catheter. RESULTS: The average time for donor operation, graf preparation and anhepatic phase was 31 minutes, 14 mi nutes and 13 minutes, respectively. The anastomosis tim for the suprahepatic vena cava, portal vein, infrahepatic ve na cava, hepatic artery and bile duct was 7 minutes, 2 mi nutes, 2 minutes, 8 minutes and 1 minute, respectively The main causes for operative mortality were pneumotho rax, anesthesia, air embolism and massive bleeding, an the successful rate of operation was 92.5%. The causes fo death after operation were stoma bleeding, infection, b liary obstruction and graft failure. CONCLUSION: The improved two-cuff technique can re duce operative mortality, enhance survival rate, and serv as an ideal method for the establishment of animal model o ROLT.

关键词： rat liver transplantation animal, model

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黄瓜矮生基因(cp)连锁的简单重复序列(SSRs)和序列标签位点(STS)标记

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农业生物技术学报 2013年第10期21卷 1152-1158页

作者：雍建朋李玉红孟永娇钟艳花程智慧陈鹏西北农林科技大学园艺学院西北农林科技大学生命学院

矮化株型是黄瓜(Cucumis sativus L.)重要的农艺性状,对于实现黄瓜的密植高效栽培和机械采收具有潜在的应用价值。矮生基因(compact gene,cp)控制着黄瓜的矮生性状,初始精细定位已经将cp基因定位在220 kb的区间,且与细胞分裂素氧化酶基... 详细信息

矮化株型是黄瓜(Cucumis sativus L.)重要的农艺性状,对于实现黄瓜的密植高效栽培和机械采收具有潜在的应用价值。矮生基因(compact gene,cp)控制着黄瓜的矮生性状,初始精细定位已经将cp基因定位在220 kb的区间,且与细胞分裂素氧化酶基因(CKX)共分离。为了进一步缩短cp基因的定位区间,本研究在原有研究基础上,以黄瓜蔓生材料WI7200(长节间)与矮生材料WI7201(短节间)为亲本,即与cp初始精细定位的F2群体(1 273株)的相同亲本,新构建了一个较大的F2群体,利用cp初始精细定位的两侧翼微卫星标记UW084680和UW084870筛选以相同亲本新构建的F2群体中的1 348个单株,共筛选出57株重组交换单株;在cp基因初始精细定位的220 kb区间内,利用黄瓜基因组测序结果及生物信息学分析,在标记区域内新开发了68对微卫星标记和1对序列标签位点(STS)标记,2对简单重复序列(SSR)标记和1对STS标记被成功用于交换单株的分析,并结合cp基因初始精细定位所用F2群体的1 273株单株的数据,最终利用2 621 F2单株将cp基因定位在178 kb的区间内,其共分离标记是SSR标记UW057998和STS标记cp-STS-6。最近的两侧翼标记CKX-indel和UW058058与cp基因分别相距0.04和0.23 cM,此结果排除了CKX作为cp候选基因的可能性,因此矮生基因的候选基因定位在标记CKX-indel和UW058058的178 kb区间,本研究结果不仅为进一步筛选和确认cp基因提供了基础数据,也为黄瓜矮生性状的分子标记辅助育种提供了科学依据。

关键词：黄瓜矮生基因(cp) 精细定位微卫星标记序列标签位点

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镉中毒大鼠睾丸与肝脏金属硫蛋白表达的时相研究

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生物化学与生物物理进展 2002年第5期29卷 811-815页

作者：任绪义周雍张建鹏冯伟华第二军医大学基础医学部生物化学与分子生物学教研室上海200433

啮齿目动物睾丸对镉毒性较肝脏更敏感 .为阐明睾丸的镉毒性分子机制 ,比较了肝脏与睾丸金属硫蛋白(MT)表达的时相变化 .mRNA采用RT PCR技术分析并用光密度扫描定量 ;蛋白质定量用ELISA方法 .结果显示 ,睾丸中存在MT ,镉中毒后MT1与MT2m... 详细信息

啮齿目动物睾丸对镉毒性较肝脏更敏感 .为阐明睾丸的镉毒性分子机制 ,比较了肝脏与睾丸金属硫蛋白(MT)表达的时相变化 .mRNA采用RT PCR技术分析并用光密度扫描定量 ;蛋白质定量用ELISA方法 .结果显示 ,睾丸中存在MT ,镉中毒后MT1与MT2mRNA明显升高 ,但MT没有相应增加 ;肝脏镉中毒后MTmRNA与MT均明显升高 .结果提示 :镉虽然能诱导睾丸MTmRNA的转录 ,但没有促进其MT的合成。

关键词：镉中毒大鼠睾丸肝脏金属硫蛋白表达时相研究

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Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

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Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu Guo Lanyue Zhang Gening Du Hao You Junfeng Huang Mudong Tu Hua Li yong peng Wei Dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 mA cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： NiFe-MIL-53 Rh Electrochemical reconstruction Catalytic mechanism Oxygen evolution reaction

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Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats

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World Journal of Gastroenterology 2025年第1期31卷 93-109页

作者： Ping Zhang Ying Zhu Pu Chen Tong Zhou Zhe-Yi Han Jun Xiao jian-Feng Ma Wen Ma peng Zang Ying Chen Graduate School Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina Key Laboratory of Aerospace Nutrition and Food Engineering China Astronaut Research and Training CenterBeijing 100094China Department of Gastroenterology The Air Force Medical CenterBeijing 100142China Department of Blood Transfusion Air Force Medical CenterBeijing 100142China

BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility *** pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal ***,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal *** cells are the pacemakers of gastrointestinal *** To investigate the effects of Bifidobacterium lactis(***)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit *** The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 *** tail group was given *** BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/***&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and *** fecal flora and mucosal flora of rats in each group were detected by 16S *** Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic *** microgravity reduced the expressions of SCF and c-kit,and increased the express

关键词： Simulated microgravity Rat Intestinal flora Gastrointestinal motility Stem cell factor/c-kit signaling pathway

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镉暴露大鼠睾丸支持细胞金属硫蛋白表达的时相研究

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生物化学与生物物理进展 2003年第6期30卷 965-968页

作者：任绪义周雍张建鹏仲燕冯伟华焦炳华第二军医大学基础医学部生物化学与分子生物学教研室上海200433

啮齿目动物的睾丸较肝脏对镉毒性更为敏感 .为阐明不同组织细胞的镉毒性分子机制 ,对镉暴露大鼠睾丸支持细胞与肝脏金属硫蛋白基因 (MT )的表达及镉蓄积进行了时相研究 .成年雄性SD大鼠低剂量镉(4 0 μmol/kg)皮下注射后 ,立即进行睾丸... 详细信息

啮齿目动物的睾丸较肝脏对镉毒性更为敏感 .为阐明不同组织细胞的镉毒性分子机制 ,对镉暴露大鼠睾丸支持细胞与肝脏金属硫蛋白基因 (MT )的表达及镉蓄积进行了时相研究 .成年雄性SD大鼠低剂量镉(4 0 μmol/kg)皮下注射后 ,立即进行睾丸支持细胞和肝脏组织的分离 .采用RT PCR技术分析mRNA ,并用光密度扫描作半定量分析 ,蛋白质定量用ELISA方法 ,原子分光光度吸收法测定镉浓度 .结果显示 :镉暴露 1h后肝脏MTmRNA即有明显的诱导表达 ,3h达高峰 ;支持细胞也有明显的诱导表达 ,6h达高峰 .镉暴露后肝脏MT有明显的诱导表达 ,但睾丸支持细胞不但未见MT增加而且还稍有下降 .提示 :a 镉对MTmRNA的诱导表达具有时间依赖性和组织特异性 .b 镉虽然能诱导睾丸MT的转录 ,但没有促进其MT的合成。

关键词：金属硫蛋白镉睾丸支持细胞睾丸组织镉损伤

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公共agent请求代理体系结构CARBA开放性验证研究

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计算机科学 2003年第2期30卷 118-120,96页

作者：张云勇彭舰刘锦德电子科技大学计算机学院微机所成都610054

After the persistent practice in the 1990's,using "Open System" idea to integrate large scale computer systems has been the consenting fact. The new mechanism based on mobile agent is discussed and the core architecture called common agent request broker architecture (CARBA)is established to embody intelligent collaboration in the next generation open system. In order to evaluate whether CARBA is open system,ODP specification is used to validate the openness of CARBA.

关键词：请求代理体系结构 CARBA 开放性验证人工智能信息处理 Agent

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