检索结果-南通市图书馆

Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第2期20卷 557-573页

作者： Jiewen Chen Xiaolin Zeng Le Wang Wenwu Zhang Gang Li Xing Cheng Peiqiang su yong Wan Xiang Li Department of Spine surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Province Key Laboratory of Orthopedics and Traumatology GuangzhouGuangdong ProvinceChina

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord *** and astrocytes play key roles in the spinal cord injury micro-environment and share a close ***,the mechanisms involved remain *** this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming *** expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord *** vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between *** further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling ***,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling *** vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function ***,Gas6 may play a role in the treatment of spinal cord *** can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

关键词： astrocytes Axl cell polarization Gas6 Hippo signal inflammatory micro-environment intercellular interaction microglia single-cell sequencing spinal cord injury

在线全文

学校读者我要写书评

暂无评论

灭鼠药致继发性凝血功能障碍13例诊治分析

中华儿科杂志 2010年第8期48卷 629-632页

作者：陈兴华戴碧涛于洁徐酉华宪莹苏庸春肖剑文温贤浩管贤敏重庆医科大学附属儿童医院血液肿瘤科 400014

目的总结灭鼠药致继发性凝血功能障碍患儿临床特征,探讨诊断及治疗措施.方法回顾分析2009-2010年13例住院治疗的灭鼠药中毒致凝血功能障碍患儿的临床资料.结果该组患儿以鼻衄、牙龈出血、皮肤瘀点、瘀斑等皮肤黏膜出血为主要临床表现... 详细信息

目的总结灭鼠药致继发性凝血功能障碍患儿临床特征,探讨诊断及治疗措施.方法回顾分析2009-2010年13例住院治疗的灭鼠药中毒致凝血功能障碍患儿的临床资料.结果该组患儿以鼻衄、牙龈出血、皮肤瘀点、瘀斑等皮肤黏膜出血为主要临床表现（66.6%）,既往身体健康,无类似出血表现.凝血像检查均有活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）延长,凝血因子Ⅱ缺乏,Ⅶ、Ⅸ、Ⅹ因子水平降低.纤维蛋白原均正常.13例患儿血、尿毒物检测,均检测出化学毒物灭鼠药（然而其中12例患儿均无明确进食灭鼠药史）.使用凝血酶原复合物,新鲜血浆和维生素K1（VitK1）治疗有效.6例患儿2～3周后再次发生出血.结论儿童继发性凝血功能障碍疾病需高度警惕抗凝血灭鼠药中毒可能.及时补充凝血因子,早期使用VitK1有效.因灭鼠药排泄慢,VitK1治疗时间宜大于2个月或更长,应随访检测凝血指标并决定是否停药.

关键词：血液凝固障碍抗啮齿动物药维生素K1 儿童

EphA2基因沉默对人食管癌EC9706细胞迁移、侵袭的影响及其机制研究

在线全文

学校读者我要写书评

暂无评论

中国现代医学杂志 2022年第1期32卷 40-46页

作者：任利兵贾如江苏春永杨晓光秦景云邯郸市中心医院胸外科河北邯郸056001

目的探讨促红细胞生成素产生肝细胞受体A2(EphA2)基因沉默对人食管癌EC9706细胞迁移、侵袭的影响及其作用机制。方法取对数生长期人食管癌EC9706细胞随机分为对照组、空载组及沉默组,分别转染Lipofectamine;2000、含无义随机序列(siRNA-... 详细信息

目的探讨促红细胞生成素产生肝细胞受体A2(EphA2)基因沉默对人食管癌EC9706细胞迁移、侵袭的影响及其作用机制。方法取对数生长期人食管癌EC9706细胞随机分为对照组、空载组及沉默组,分别转染Lipofectamine;2000、含无义随机序列(siRNA-NC-PGCsi3.0)和EphA2干扰序列(EphA2-siRNA-PGCsi3.0)质粒和脂质体的复合物。稳定转染后,Brd U法检测细胞增殖能力,划痕实验检测细胞迁移能力,Transwell小室实验检测细胞侵袭能力,qRT-PCR和Western blotting分别检测细胞中EphA2、Wnt1、β-连环蛋白(β-catenin)、E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)mRNA及蛋白相对表达量。结果对照组、空载组、沉默组BrdU阳性率分别为(26.48±2.79)%、(23.52±2.57)%、(13.29±2.06)%,Brd U阳性率组间比较,差异有统计学意义(F=38.560,P=0.000)。与对照组、空载组比较,沉默组Brd U阳性率降低(P 0.05)。对照组、空载组、沉默组细胞划痕愈合率分别为(83.16±8.31)%、(82.35±7.24)%、(34.24±5.27)%,细胞划痕愈合率组间比较,差异有统计学意义(F=78.869,P=0.000)。与对照组、空载组比较,沉默组细胞划痕愈合率降低(P 0.05)。对照组、空载组、沉默组穿膜细胞数分别为(326.74±33.15)个、(331.27±34.59)个、(126.23±26.18)个,穿膜细胞数组间比较,差异有统计学意义(F=68.997,P=0.000),与对照组、空载组比较,沉默组穿膜细胞数减少(P 0.05)。细胞中EphA2、Wnt1、β-catenin、Ecadherin、Vimentin mRNA相对表达量比较,差异有统计学意义(P 0.05)。结论 EphA2基因沉默对人食管癌EC9706细胞迁移和侵袭有一定的抑制作用,其机制可能与阻止Wnt/β-catenin信号通路相关。

关键词：食管肿瘤促红细胞生成素产生肝细胞受体A2 迁移侵袭

Glucocorticoid receptor signaling in the brain and its involvement in cognitive function

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第9期20卷 2520-2537页

作者： Chonglin su Taiqi Huang Meiyu Zhang Yanyu Zhang Yan Zeng Xingxing Chen Brain Science and Advanced Technology Institute Hubei Province Key Laboratory of Occupational Hazard Identification and ControlSchool of MedicineWuhan University of Science and TechnologyWuhanHubei ProvinceChina

The hypothalamic-pituitary-adrenal axis regulates the secretion of glucoco rticoids in response to environmental *** the brain,a nuclear receptor transcription fa ctor,the glucocorticoid recepto r,is an important component of the hypothalamicpituitary-a d renal axis's negative feedback loop and plays a key role in regulating cognitive equilibrium and *** glucoco rticoid receptor influences cognitive processes,including glutamate neurotransmission,calcium signaling,and the activation of brain-derived neurotrophic factor-mediated pathways,through a combination of genomic and non-genomic *** interactions within the central nervous system can alter the expression and activity of the glucocorticoid receptor,there by affecting the hypothalamic-pituitary-a d renal axis and stress-related cognitive *** appropriate level of glucocorticoid receptor expression can improve cognitive function,while excessive glucocorticoid receptors or long-term exposure to glucoco rticoids may lead to cognitive *** with cognitive impairment-associated diseases,such as Alzheimer's disease,aging,depression,Parkinson's disease,Huntington's disease,stroke,and addiction,often present with dysregulation of the hypothalamic-pituitary-adrenal axis and glucocorticoid receptor *** review provides a comprehensive overview of the functions of the glucoco rticoid receptor in the hypothalamic-pituitary-a d renal axis and cognitive *** emphasizes that appropriate glucocorticoid receptor signaling fa cilitates learning and memory,while its dysregulation can lead to cognitive *** provides clues about how glucocorticoid receptor signaling can be targeted to ove rcome cognitive disability-related disorders.

关键词： brain-derived neurotrophic factor calcium signaling glucocorticoid receptor glucocorticoid glutamate transmission hypothalamic-pituitary-adrenal axis long-term potentiation neurocognitive disorders neuroplasticity stress

在线全文

学校读者我要写书评

暂无评论

儿童急性淋巴细胞白血病单中心临床研究

中华儿科杂志 2009年第12期47卷 939-941页

作者：梁筱灵宪莹戴碧涛徐酉华苏庸春王世一陆玲玲李欣于洁重庆医科大学附属儿童医院血液肿瘤科 400014

目的初步评估所采用的2004全国小儿血液病学术会议关于儿童急性淋巴细胞白血病诊疗建议方案（简称04方案）的诊断、治疗效果。方法对2004年10月-2007年6月282例急性淋巴细胞性白血病（简称急淋）患儿，参照04方案诊疗建议进行诊断、分... 详细信息

目的初步评估所采用的2004全国小儿血液病学术会议关于儿童急性淋巴细胞白血病诊疗建议方案（简称04方案）的诊断、治疗效果。方法对2004年10月-2007年6月282例急性淋巴细胞性白血病（简称急淋）患儿，参照04方案诊疗建议进行诊断、分型及治疗；按危险度分型进行统计并用SPSS统计软件对结果进行生存分析。结果2004年10月-2007年6月88例新诊急淋患儿接受了04方案化疗，总完全缓解（CR）率为91．30％（63／69），标、中危组CR率均为100％（37／37），高危组CR率为81．25％（26／32）；总4年无病生存率（EFS）为（59．73±7．22）％，标、中危患儿的EFS分别为（75．60±9．71）％和（65．50±11．69）％，高危组EFS为（44．03±12．36）％；总复发率为18．18％，骨髓复发占87．50％，单纯的中枢复发占12．50％；化疗相关死亡率为9．09％，其中诱导缓解治疗阶段因感染死亡7例（7．95％），真菌是主要病原菌。结论应用04方案对儿童急淋进行诊治疗效满意；化疗第19天（d19）骨髓幼稚细胞数和临床危险度分型是独立的预后指标，大剂量甲氨蝶呤对中枢神经系统白血病的预防起到了重要作用；诱导期化疗过强，化疗相关死亡率高。

关键词：儿童白血病淋巴细胞急性回顾性研究

Single-cell and spatial omics:exploring hypothalamic heterogeneity

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第6期20卷 1525-1540页

作者： Muhammad Junaid Eun Jeong Lee su Bin Lim Department of Biochemistry&Molecular Biology Ajou University School of MedicineSuwonSouth Korea Department of Biomedical Sciences Graduate School of Ajou UniversitySuwonSouth Korea Department of Brain Science Ajou University School of MedicineSuwonSouth Korea

Elucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions. Over the past decade, single-cell and spatial omics technologies have significantly evolved, overcoming initial technical challenges in capturing and analyzing individual cells. These high-throughput omics technologies now offer a remarkable opportunity to comprehend the complex spatiotemporal patterns of transcriptional diversity and cell-type characteristics across the entire hypothalamus. Current single-cell and single-nucleus RNA sequencing methods comprehensively quantify gene expression by exploring distinct phenotypes across various subregions of the hypothalamus. However, single-cell/single-nucleus RNA sequencing requires isolating the cell/nuclei from the tissue, potentially resulting in the loss of spatial information concerning neuronal networks. Spatial transcriptomics methods, by bypassing the cell dissociation, can elucidate the intricate spatial organization of neural networks through their imaging and sequencing technologies. In this review, we highlight the applicative value of single-cell and spatial transcriptomics in exploring the complex molecular-genetic diversity of hypothalamic cell types, driven by recent high-throughput achievements.

关键词： cellular diversity hypothalamus multi-omics single-cell transcriptomics spatial transcriptomics

Statistical characteristics and classification of ionospheric mid-latitude trough as revealed by the observations of DMSP-F18

在线全文

学校读者我要写书评

暂无评论

Earth and Planetary Physics 2025年第1期9卷 137-147页

作者： Yi Liu JiuHou Lei FuQing Huang su Zhou School of Earth and Space Sciences University of Science and Technology of ChinaHefei 230026China School of Science Guiyang UniversityGuiyang 550005China

Statistical characteristics and the classification of the topside ionospheric mid-latitude trough are systemically analyzed,using observations from the Defense Meteorological Satellite Program F18(DMSP-F18)*** data was obtained at an altitude of around 860 km in near polar orbit,throughout *** study identified the auroral boundary based on the in-situ electron density and electron spectrum,allowing us to precisely determine the location of the mid-latitude *** differs from most previous works,which only use Total Electron Content(TEC)or in-situ electron *** our study,the troughs exhibited a higher occurrence rate in local winter than in summer,and extended to lower latitudes with increasing geomagnetic *** was found that the ionospheric mid-latitude trough,which is associated with temperature changes or enhanced ion drift,exhibited distinct ***,the ionospheric mid-latitude troughs related to electron temperature(Te)peak were located more equatorward of auroral oval boundary in winter than in *** ionospheric mid-latitude troughs related to Te-maximum were less frequently observed at 60−70°S magnetic latitude and 90−240°E ***,the troughs related to ion temperature(Ti)maximums were observed at relatively higher latitudes,occurring more frequently in *** addition,the troughs related to ion velocity(Vi)maximums could be observed in all *** troughs with the maximum-Ti and maximum-Vi were located closer to the equatorward boundary of the auroral oval at the nightside,and in both *** implies that enhanced ion drift velocity contributes to increased collisional frictional heating and enhanced ion temperatures,resulting in a density depletion within the trough region.

关键词： topside ionosphere mid-latitude trough ionospheric main trough auroral oval boundary temperature maximum

Molecular Dynamics Simulation Studies on the Micromorphology and Proton Transport of Nafion/Ti_(3)C_(2)T_(x) Composite Membrane

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2024年第3期42卷 373-387,I0009页

作者： Zhi-Yue Han su-Peng Pei chun-Yang Yu yong-Feng Zhou School of Chemical and Environmental Engineering Shanghai Institute of TechnologyShanghai 201418China School of Chemistry&Chemical Engineering Frontiers Science Center for Transformative MoleculesShanghai Key Laboratory of Electrical Insulation and Thermal AgingShanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Green and High-end Utilization of Salt Lake Resources Chinese Academy of SciencesXining 810008China

The perfluorosulfonic acid(PFSA) membrane doped with two-dimensional conductive filler Ti_(3)C_(2)T_(x) is a fuel cell proton exchange membrane with high application potential. Experimental studies showed that the proton conductivity of Nafion/Ti_(3)C_(2)T_(x) composite membrane is improved significantly compared with that in pure Nafion. However, the microscopic mechanism of doping on the enhancement of membrane performance is remain unclear now. In this work, molecular dynamics simulation was used to investigate the microscopic morphology and proton transport behaviors of Nafion/Ti_(3)C_(2)T_(x) composite membrane at the molecular level. The results shown that there were significant differences about the diffusion kinetics of water molecules and hydroxium ions in Nafion/Ti_(3)C_(2)T_(x) at low and high hydration levels in the nanoscale *** the increase of water content, Ti_(3)C_(2)T_(x) in membrane was gradually surrounded by ambient water molecules to form a hydration layer, and forming a relatively continuous proton transport channel between Nafion polymer and Ti_(3)C_(2)T_(x) monomer. The continuous proton transport channel could increase the number of binding sites of proton and thus achieving high proton conductivity and high mobility of water molecules at higher hydration level. The current work can provide a theoretical guidance for designing new type of Nafion composite membranes.

关键词： Nafion/Ti_(3)C_(2)T_(x)composite membrane Proton transport Molecular dynamics simulation

Interfacing biosynthetic CdS with engineered Rhodopseudomonas palustris for efficient visible light-driven CO_(2)-CH_(4) conversion

在线全文

学校读者我要写书评

暂无评论

Frontiers of Chemical Science and Engineering 2024年第10期18卷 1-12页

作者： Yu Zhang Yulei Qian Zhenye Tong su Yan Xiaoyu yong Yang-chun yong Jun Zhou Bioenergy Research Institute College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816China Biofuels Institute Jiangsu Collaborative Innovation Center of Technology and Material of Water TreatmentSchool of Emergency Management&School of Environment and Safety EngineeringJiangsu UniversityZhenjiang 212013China

Engineered photosynthetic bacterium Rhodo-pseudomonas palustris is excellent at one-step CO_(2) biomethanation and can use near-infrared light sources,overcoming the limitations of conventional photosynthetic *** current study constructed a biohybrid system that deposited CdS nanoparticles on *** biohybrid system broadens the capture of sustainable solar energy,achieving a 155 nmol-mL-biological CH,production under full visible light irradiation,13.4-fold of that by the pure *** transcriptome profiles revealed that gene expression related to photosynthetic electron transfer chain,nitrogenase,nanofilaments,and redox stress defense was ***,we attributed the much-enhanced CO_(2) biomethanation in the biohybrid system to the remarkable increase in the intracellular reducing power and the stronger rigidity of the cells assisted by photoexcited electrons from CdS *** discovery offers insight and a promising strategy forimproving the current CO_(2)-CH_(4) biomanufacturing system.

关键词： CO_(2)methanation Rhodopseudomonas palustris CdS nanoparticles green catalysis