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Recovery of photosynthesis,sucrose metabolism,and proteolytic enzymes in Kandelia obovata from rare cold events in the northernmost mangrove,China

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Ecological Processes 2016年第1期5卷 108-119页

作者： Chunfang Zheng yong ye Weicheng Liu Jianwu Tang Chengnian Zhang Jianbiao Qiu Jinong Chen Wenzhou Ocean Research Institute Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resourceZhejiang Mariculture Research InstituteWenzhou 325000China Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem College of the Environment and EcologyXiamen UniversityXiamenChina Nanji Islands National Marine Nature Reserve Administration PingyangChina Wenzhou Vocational College of Science and Technology WenzhouChina

Introduction:Understanding how mangroves respond to rare cold events has implications for both restoration and conservation under climate change *** study investigated the responses of photosynthesis and activities of key enzymes involving carbon and nitrogen metabolism at different ages of Kandelia obovata to a rare cold event in the winter of ***:This study took place on Ximen Island,Zhejiang Province,*** measured the physiological recovery of 2-3-,5-6-,9-10-and 54-55-year-old *** trees after freezing injury in February and March in 2011 and 2012,***:Chilling injury index and electrolyte leakage of *** increased with increasing tree age in the winter of 2010,and electrolyte leakage in *** at different ages in the winter of 2010 was far higher than that in the winter of *** rare cold events significantly changed the recoveries of the leaf net photosynthetic rate(Pn)and stomatal conductance(Gs);ratios of chlorophyll a/chlorophyll b(Chl a/Chl b);contents of total soluble sugar(TSS),sucrose,free amino acid(FAA),and soluble protein;and activities of sucrose phosphate synthase(SPS),endopeptidase,and carboxypeptidase in *** at different *** effects were mainly due to changes in the physiological mechanism in the 2-year-old trees.A clear decrease in Pn of the 2-year-old trees was observed in February 2011,as exemplified by reductions in ratios of Chl a/Chl b and chlorophyll/carotenoid(Chl/Car),as well as inhibition of the levels of TSS and FAA(osmotic substances).During recovery in 2011 and 2012,the activities of SPS and sucrose synthase(SS)were responsible for sucrose synthesis after the rare cold events in 2011,but only SPS activity was one of the main factors contributing to the metabolism of stachyose to sucrose without cold damage in *** played a more important role than endopeptidase during protein hydrolysis after the rare cold ***:The results suggest that

关键词： Kandelia obovata Photosynthesis Sucrose synthase Rare cold events Peptidase

Mechanisms on inhibition of photosynthesis in Kandelia obovata due to extreme cold events under climate change

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Ecological Processes 2016年第1期5卷 250-260页

作者： Chunfang Zheng Jianwu Tang Jinong Chen Weicheng Liu Jianbiao Qiu Xin Peng yong ye Zhejiang Mariculture Research Institute Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resourceWenzhou 325005ZhejiangPeople’s Republic of China Nanji Islands National Marine Nature Reserve Administration Pingyang 325401People’s Republic of China Key Laboratory of the Ministry of Eduction for Coastal and Wetland Ecosystem College of the Environment and EcologyXiamen UniversityXiamen 361005People’s Republic of China

Introduction:Mangroves that grow at the latitudinal extremes of their distribution are susceptible to extreme cold *** enhancement of low temperature stress(seLTS)is a typical characteristic of extreme cold *** temperature stress can inhibit mangrove photosynthesis,which often inhibits the growth and development of ***,the possible reasons for impairment to photosynthesis of mangroves due to extreme cold events remain ***:Kandelia obovata seedlings in a growth chamber were exposed to 5℃/−2℃(day/night)for 36 h(−2℃ for 16 h)with 12 h light per day at 600μmol m^(−2) s^(−1) photosynthetic photon flux density(PPFD)(a low temperature stress,aLTS),then the plants were transferred to the control condition(15℃/10℃(day/night)and allowed to recover for 5 days(R1).The other seedlings were subjected to low temperature treatment with a day/night temperature of 5℃/−1℃ in a growth chamber for 24 *** these plants were transferred to 5℃/−2℃(day/night)under the same light and climate conditions for 36 h(two low temperature stresses,tLTS).Following the successive enhancement of low temperature treatment,these plants were returned to 15℃/10℃(day/night)for another 5-day recovery period(R2).Results:Results showed that aLTS treatment significantly reduced leaf net photosynthetic rate(P_(n))and stomatal conductance(G_(s)),while increased intercellular CO_(2) concentration(C_(i)).Photosynthetic activity of leaves quickly recovered after the plants were returned to control temperature for 5 days(R1).However,decreases in leaf Pn were more obvious under tLTS treatment than those under aLTS *** reduced Pn might be attributed to stomatal and non-stomatal ***,non-stomatal limitation played a major role in reducing Pn during tLTS treatment,as proven by reduced ribulose 1,5-biphosphate carboxylase(RuBPCase)*** limitation also enhanced lipid peroxidation in chloroplasts,decreased sucrosemetabolizing enzymes and ra

关键词： Kandelia obovata Low temperature Gas exchange Sucrose synthesis Peptidase

Rational design of a bifunctional fluorescent probe for distinguishing Hcy/Cys from GSH with ideal properties

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Chinese Chemical Letters 2021年第3期32卷 1061-1065页

作者： Xiaojie Ren Lide Liao Zhaoguang Yang Haipu Li Xi Li Yangang Wang yong ye Xiangzhi Song College of Chemistry&Chemical Engineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety Changsha 410083China College of Biological Chemical Science and Engineering Jiaxing UniversityJiaxing 314001China College of Chemistry and Molecular Engineering Zhengzhou UniversityZhengzhou 450001China

By pairing two fluoropho res according to their optical prope rties such as absorption spectral overlap and absorptivity,fluorescent quantum yield and emission spectral separation,a bifunctional fluorescent probe,TQBF-NBD,was rationally designed and synthesized to discriminatively sense Hcy/Cys and GSH with good selectivity and *** is noted that this probe could work under a single-wave length excitation and displayed a mega-large Stokes ***-NBD reacted with Hcy/Cys to give a mixed green-red fluorescence and displayed a red fluorescence upon the treatment with *** imaging of intracellular Hcy/Cys from GSH with the help of TQBF-NBD was realized in living cells and zebrafish.

关键词： Borate complex Red-emitting Mega-large stokes shift Fluorescent probe

Analysis and optimization of performance parameters of the^(220)Rn chamber in flow-field mode using computational fluid dynamics method

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Nuclear Science and Techniques 2024年第10期35卷 70-85页

作者： Shao-Hua Hu yong-Jun ye Zheng-Zhong He De-Tao Xiao Xiang-Yu Xu Jian-Kai Wang Qing-Zhi Zhou School of Nuclear Science and Technology University of South ChinaHengyang 421001China School of Resources Environment and Safety Engineering University of South ChinaHengyang 421001China

The impact of the radiation dose produced by^(222)Rn/^(220)Rn and its progeny on human health has garnered increasing interest in the nuclear research *** establishment of robust,regulatory,and competent^(220)Rn chambers is crucial for accurately measuring radioactivity ***,studying the uniformity of the^(220)Rn progeny through experimental methods is challenging,because measuring the concentration of^(220)Rn and its progeny in multiple spatial locations simultaneously and in real time using experimental methods is ***,achieving precise control of the concentration of^(220)Rn and its progeny as well as the reliable sampling of the progeny pose significant *** solve this problem,this study uses computational fluid dynamics to obtain the flow-field data of the^(220)Rn chamber under different wind speeds and progenyreplenishment *** analysis of the concentration distribution of the progeny and quantitative analysis of the progeny concentration and uniformity of the progeny concentration are *** research findings indicated that the progeny concentration level is primarily influenced by wind speed and the progeny-complement *** speed also plays a crucial role in determining progeny concentration uniformity,whereas the progeny-complement rate has minimal impact on *** ensure the accuracy of^(220)Rn progeny concentration sampling,we propose a methodology for selecting an appropriate sampling area based on varying progeny *** study holds immense importance for enhancing the regulation and measurement standards of^(220)Rn and its progeny.

关键词： Thoron Progeny Regulatory CFD simulation

FACE RECOGNITION BASED ON WAVELET-CURVELET-FRACTAL TECHNIQUE

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Journal of Electronics(China) 2010年第2期27卷 206-211页

作者： Zhang Zhong Zhuang Peidong Liu yong Ding Qun ye Hong＇an Department of Electronic Engineering Heilongjiang University Harbin 150080 China

In this paper,a novel face recognition method,named as wavelet-curvelet-fractal technique,is proposed. Based on the similarities embedded in the images,we propose to utilize the wave-let-curvelet-fractal technique to extract facial features. Thus we have the wavelet’s details in diagonal,vertical,and horizontal directions,and the eight curvelet details at different angles. Then we adopt the Euclidean minimum distance classifier to recognize different faces. Extensive comparison tests on dif-ferent data sets are carried out,and higher recognition rate is obtained by the proposed technique.

关键词： Face recognition Wavelet decomposition Curvelet transform Fractal Facial feature extraction

Synthesis and Cleavage Activity of Artifical Minic Polypeptides

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Chinese Chemical Letters 2006年第9期17卷 1197-1200页

作者： yong ye Xiao Lian HU Ping LI Ming Yu NIU Li Feng CAO Yu Fen ZHAO Department of Chemistry Key Laboratory of Chemical Biology and Organic Chemistry Zhengzhou University Zhengzhou 450052 Key laboratory of Bioorganic Phosphorus Chemistry ＆ Chemical Biology （Ministry of Education） Department of Chemistry Tsinghua University Beijing 100084

Two artificial minic polypeptides which are synthetic analogues of natural products with DNA affinity were synthesized, and theirs cleavage activity with DNA were examined. The structures of these compounds was confir... 详细信息

关键词： DNA artificial minic polypeptide cleavage agents.

Largest dinosaur tracksite in China (Cretaceous, Zhaojue area, Sichuan Province): On the verge of destruction

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Geoscience Frontiers 2021年第5期12卷 124-137页

作者： Lida Xing Martin G.Lockley Hendrik Klein W.Scott Persons IV Guangzhao Peng yong ye Miaoyan Wang State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesBeijing 100083China School of the Earth Sciences and Resources China University of GeosciencesBeijing 100083China Dinosaur Trackers Research Group University of ColoradoDenver 80217USA Saurierwelt Paläontologisches Museum Alte Richt 7D-92318 NeumarktGermany Mace Brown Museum of Natural History Department of Geology and Environmental GeosciencesCollege of CharlestonCharleston 29401USA Zigong Dinosaur Museum ZigongSichuan ProvinceChina

The Zhaojue(ZJ)tracksites represent multiple steeply-inclined,track-bearing exposures in the clastic Feitianshan Formation of Sichuan Province that have been sequentially,excavated,mapped,expanded and destroyed,by quarrying,erosion or collapse,for almost three *** quarried area extends for more almost 1.0 km from north to south and-0.5 km from east to *** important track-bearing surfaces have been identified and identified as ZJ-Ⅰ,ZJ-Ⅱ,ZJ-ⅡN and ZJ-Ⅲ,some of which are newly exposed surfaces or expanded surfaces that were previously partially *** represent surfaces lost since they were first reported in papers published in *** ZJ-Ⅱ site represents a hugely expanded continuation of a much smaller track-bearing surface described in *** was mapped using drone technology,and now represents the largest tracksite in China,with a minimum count of 933 recognizable tracks,and the longest recorded sauropod and ornithopod trackways from China(-80 m and-52 m respectively),which also reveal a pronounced change in the direction of trackmaker *** ZJ-Ⅱ surface represents the best Zhaojue paleo-census sample giving a count of 68 individuals from 61 trackways(37 ornithopod,10 theropod,14 sauropod)and isolated tracks(representing 7 individuals).Thus,ornithopods(both large and small trackmakers)represent-54%of the total number of *** smaller ZJ-Ⅲ surface was mapped using traditional methods,and reveals at least 6 ornithopod-and 4 theropod *** the ZJ-Ⅱ and ZJ-Ⅲ surfaces reveal parallel ornithopod trackways suggestive of social or gregarious *** combined data from all four Zhaojue surfaces reveal a total of 1928 tracks,and include a few previously reported pterosaurs and theropod swim *** Zhaojue quarry complex provide a good example of multiple track-bearing sites(surfaces)that require long term study and monitoring to extract as much trackway data as possible before in situ physical evidence is lost.

关键词： Feitianshan Formation Onithopod Teropod Suropod

The Application of the Trial-Discovery Model to English Grammar Teaching in Senior High Schools

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Sino-US English Teaching 2012年第2期9卷 924-936页

作者： ye yong-mei Zhejiang Lishui High School Lishui China

In senior high schools, great importance and attention are required to be attached to grammar teaching and learning. However, a majority of teachers either apply the traditional method or neglect grammar teaching and learning, both of which go against the ideas of the new curriculum of the high school. To solve the problem, based on the constructionist theory, Halliday＇s theory of register, and the trial-discovery model, the trial-discovery English grammar teaching model was constructed. To find out whether this teaching model is more effective than the traditional grammar teaching, an experimental study of the trial-discovery model in senior English grammar teaching is carried out. This study deals with the problem how we can improve the efficiency of grammar teaching, that is by increasing the students＇ learning interest and improving the accuracy in English writing. After the analysis of qualitative and quantitative data collected through questionnaires, interviews, and writing tests, the study reveals that the trial-discovery grammar teaching is more effective to stimulate the students＇ interest in learning grammar, and is more helpful to improve the accuracy and complexity in English writing than the traditional grammar teaching

关键词： English grammar senior high school trial-discovery model teaching efficiency

Quantitative parameters in novel spectral computed tomography:Assessment of Ki-67 expression in patients with gastric adenocarcinoma

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World Journal of Gastroenterology 2023年第10期29卷 1602-1613页

作者： Li-Ting Mao Wei-Cui Chen Jian-ye Lu Han-Liang Zhang yong-Song ye Yu Zhang Bo Liu Wei-Wei Deng Xian Liu Department of Radiology The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510120Guangdong ProvinceChina Department of Pathology The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510120Guangdong ProvinceChina Department of Scientific Research Clinical&Technical SupportPhilips Healthcare ChinaShanghai 200040China

BACKGROUND The level of Ki-67 expression has served as a prognostic factor in gastric *** quantitative parameters based on the novel dual-layer spectral detector computed tomography(DLSDCT)in discriminating the Ki-67 expression status are *** To investigate the diagnostic ability of DLSDCT-derived parameters for Ki-67 expression status in gastric carcinoma(GC).METHODS Dual-phase enhanced abdominal DLSDCT was performed preoperatively in 108 patients with gastric *** tumor monoenergetic CT attenuation value at 40-100 kilo electron volt(kev),the slope of the spectral curve(λ_(HU)),iodine concentration(IC),normalized IC(nIC),effective atomic number(Z^(eff))and normalized Z^(eff)(nZ^(eff))in the arterial phase(AP)and venous phase(VP)were retrospectively compared between patients with low and high Ki-67 expression in gastric ***’s correlation coefficient was used to analyze the association between the above parameters and Ki-67 expression *** operating characteristic(ROC)curve analysis was performed to compare the diagnostic efficacy of the statistically significant parameters between two *** Thirty-seven and 71 patients were classified as having low and high Ki-67 expression,***_(40 kev-VP),CT_(70 kev-VP),CT_(100 kev-VP),and Z^(eff)-related parameters were significantly higher,but IC-related parameters were lower in the group with low Ki-67 expression status than the group with high Ki-67 expression status,and other analyzed parameters showed no statistical difference between the two ***’s correlation analysis showed that CT_(40 kev-VP),CT_(70 kev-VP),CT_(100 kev-VP),Z^(eff),and n Z^(eff) exhibited a negative correlation with Ki-67 status,whereas IC and nIC had positive correlation with Ki-67 *** ROC analysis demonstrated that the multi-variable model of spectral parameters performed well in identifying the Ki-67 status[area under the curve(AUC)=0.967;sensitivity 9

关键词： Spectral computer tomography Quantitative parameters Gastric carcinoma Iodine concentration Effective atomic number Ki-67 expression