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Transplantation of VEGF165-gene-transfected endothelial progenitor cells in the treatment of chronic venous thrombosis in rats

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Chinese Medical Journal 2010年第4期123卷 471-477页

作者： MENG Qing-you LI xiao-qiang YU xiao-bin LEI Feng-rui JIANG Kun LI Chuan-yong Department of Vascular Surgery Second Affiliated Hospital of Suzhou University Suzhou Jiangsu 215004 China

Background The organization and recanalization of thrombi is a dynamic and complex process. The aim of this research was to study the cotherapeutic effect of stem cell transplantation and gene transfection on chronic venous thrombosis. Methods We constructed a recombinant adenoviral vector carrying the vascular endothelial growth factor 165 （VEGF165） gene by using the pAdEasy system, which was subsequently identified and amplified. Simultaneously, endothelial progenitor cells （EPCs） were isolated from rat bone marrow using Ficoll, cultured in EBM-2MV medium, and identified. Then, the cells were transfected with the recombinant Ad-VEGF165. The EPCs were labeled with 1 ,1＇-dioctadecyl-3,3,3＇,3＇-tetramethylindocarbocyanine （Dil） before transplantation. A rat model of chronic vein thrombosis was developed by partial ligation of the inferior vena cava. The rats were randomly divided into 4 groups （n=25, each）： A, Ad-VEGF165/EPC-transplantation group received 1 ml （10^6） of Ad-VEGF165/EPCs; B, EPC-transplantation group received 1 ml （10^6） of EPCs; C, Ad/EPC-transplantation group received 1 ml （10^6） of Ad/EPCs; D, control group received 1 ml of the transplantation medium. The thrombi and adjacent caval walls were harvested 28 days after transplantation; real-time quantitative polymerase chain reaction was used to detect the expression level of vascular endothelial growth factor （VEGF） mRNA; and western blotting was used to measure changes in VEGF protein expression. Hematoxylin-eosin staining and immunohistochemical staining were performed to detect recanalization. Neovascularization was detected by immunohistochemical staining using the antibody for von Willebrand factor （vWF）, which is a component of endothelial *** capillary density was quantitatively determined by counting the capillaries under a high-power microscope. Results The Ad-VEGF165 was constructed, and bone-marrow-derived EPCs were cultivated and successfully identified. We determined the optimum tran

关键词： vascular endothelial growth factor endothelialprogenitor cell gene transfection adenovirus venous thrombosis

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一测多评法同时测定木瓜中2种常见三萜酸

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中成药 2013年第4期35卷 770-773页

作者：张勇张玲谢晓梅安徽中医学院药学院现代中药安徽省重点实验室安徽合肥230031

目的建立"一测多评"法同时测定木瓜中齐墩果酸、熊果酸2种常见三萜酸成分。方法以齐墩果酸为内参物,考察HPLC和UPLC两种色谱体系,建立相对校正因子计算熊果酸的量,并与外标法(实测值)进行比较。结果在不同色谱分析体系中,熊果酸相对校... 详细信息

目的建立"一测多评"法同时测定木瓜中齐墩果酸、熊果酸2种常见三萜酸成分。方法以齐墩果酸为内参物,考察HPLC和UPLC两种色谱体系,建立相对校正因子计算熊果酸的量,并与外标法(实测值)进行比较。结果在不同色谱分析体系中,熊果酸相对校正因子及保留时间比值的重现性良好,RSD分别为1.4%和0.51%。不同产地木瓜药材及饮片熊果酸计算值与实测值偏差范围较小(相对偏差范围:-3.3%～2.1%),Pearson相关系数0.9997(P<0.01)。结论采用一测多评法分析定量木瓜中2种常见三萜酸的可靠性良好。

关键词：齐墩果酸熊果酸一测多评 HPLC UPLC

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Influential factors and control of water inrush in a coal seam as the main aquifer

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International Journal of Mining Science and Technology 2018年第2期28卷 187-193页

作者： Gao Rui Yan Hao Ju Feng mei Xianchen Wang Xiulin Key Laboratory of Deep Coal Resource Mining Ministry of Education School of Mines China University of Mining and Technology State Key Laboratory for Geomechanics & Deep Underground Engineering China University of Mining and Technology Huadian Coal Industry Group Co. Ltd.

In this paper, a combination of field measurement, theoretical analysis and numerical simulation were used to study the main control factors of coal mine water inrush in a main aquifer coal seam and its control scheme. On the basis of revealing and analyzing the coal seam as the main aquifer in western coal mine of xiao Jihan coal mine, the simulation software of PHASE-2D was applied to analyze the water inflow under different influencing factors. The results showed that water inflow increases logarithmically with the coal seam thickness, increases as a power function with the permeability coefficient of the coal seam, and increases linearly with the coal seam burial depth and the head pressure; The evaluation model for the factors of coal seam water inrush was gained by using nonlinear regression analysis with SPSS. The mine water inrush risk evaluation partition within the scope of the mining field was obtained,through the engineering application in xiao Jihan coal mine. To ensure the safe and efficient production of the mine, we studied the coal mine water disaster prevention and control measures of a main aquifer coal seam in aspects of roadway driving and coal seam mining.

关键词： Coal seam Main aquifer Water inrush Influential factors Control

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基于风电机组控制原理的风功率数据识别与清洗方法

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动力工程学报 2021年第4期41卷 316-322,329页

作者：梅勇李霄胡在春姚惠刘达国家电投集团能源科技工程有限公司上海201100 国家电投集团广西电力有限公司南宁530003 中国核科技信息与经济研究院北京100048

针对变速变桨距控制风电机组在风功率曲线拟合中所面临的非正常运行数据识别与清洗难题,提出了一种基于风电机组控制原理的风功率数据识别与清洗方法。从控制原理出发,首先通过有功功率和发电机转速对风功率散点进行预处理,然后分析功... 详细信息

针对变速变桨距控制风电机组在风功率曲线拟合中所面临的非正常运行数据识别与清洗难题,提出了一种基于风电机组控制原理的风功率数据识别与清洗方法。从控制原理出发,首先通过有功功率和发电机转速对风功率散点进行预处理,然后分析功率时序分布,识别出限功率点,最后结合四分位法对叶尖速比散点和风功率散点进行清洗,在完成功率散点筛选的同时,识别出机组在不同风速下的运行状态。结果表明:本文方法对不同形态特征的风功率散点都具有很好的清洗效果,而且算法执行效率高。

关键词：风功率控制原理叶尖速比四分位法

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Serological investigation of IgG and IgE antibodies against food antigens in patients with inflammatory bowel disease

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World Journal of Clinical Cases 2019年第16期7卷 2189-2203页

作者： Hai-Yang Wang Yi Li Jia-Jia Li Chun-Hua Jiao xiao-Jing Zhao Xue-Ting Li mei-Jiao Lu Xia-Qiong Mao Hong-Jie Zhang Department of Gastroenterology The Affiliated Sir Run Run HospitalNanjing Medical UniversityNanjing 211100Jiangsu ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina

BACKGROUND Food antigens have been shown to participate in the etiopathogenesis of inflammatory bowel disease (IBD),but their clinical value in IBD is still *** To analyze the levels of specific immunoglobulin G (IgG) and E (IgE) antibodies against food antigens in IBD patients and to determine their clinical value in the pathogenesis of *** We performed a retrospective study based on patients who visited the First Affiliated Hospital of Nanjing Medical University between August 2016 and January 2018.A total of 137 IBD patients,including 40 patients with ulcerative colitis (UC) and 97 patients with Crohn’s disease (CD),and 50 healthy controls (HCs),were *** food-specific IgG antibodies were detected by semiquantitative enzyme-linked immunosorbent assay,and serum food-specific IgE antibodies were measured by Western *** value of food-specific IgG antibodies was compared among different groups,and potent factors related to these antibodies were explored by binary logistic *** Food-specific IgG antibodies were detected in 57.5% of UC patients,in 90.72% of CD patients and in 42% of HCs.A significantly high prevalence and titer of foodspecific IgG antibodies were observed in CD patients compared to UC patients and *** number of IgG-positive foods was greater in CD and UC patients than in HCs (CD vs HCs,P = 0.000;UC vs HCs,P = 0.029).The top five food antigens that caused positive specific IgG antibodies in CD patients were tomato (80.68%),corn (69.32%),egg (63.64%),rice (61.36%),and soybean (46.59%).The foods that caused positive specific IgG antibodies in UC patients were egg (60.87%),corn (47.83%),tomato (47.83%),rice (26.09%),and soybean (21.74%).Significantly higher levels of total food-specific IgG were detected in IBD patients treated with anti-TNFα therapy compared to patients receiving steroids and immunosuppressants (anti-TNFα vs steroids,P = 0.000;anti-TNFα vs immunosuppressants,P = 0.000;anti-TNFα vs steroids

关键词： Inflammatory bowel disease Food-specific immunoglobulin G Food intolerance

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Application of Ginkgo biloba L. Extract at the Early Stage of Tumor Development Helps Cyclophosphamide Inhibit the Growth of Tumor Cells

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Biomedical and Environmental Sciences 2021年第12期34卷 1010-1014页

作者： YANG Jing Hui MENG Fan Zhu BESELER Cheryl L LI Hao LIU xiao mei GUO Yin Pei QIN Li Li ZUO Ru Yue REN Shu Ping Department of Liver Gallbladder and PancreasChina-Japan Union HospitalJilin UniversityChangchun 130030JilinChina Department of Occupational and Environmental Health School of Public HealthJilin UniversityChangchun 130021JilinChina Department of Environmental Agricultural and Occupational HealthCollege of Public HealthUniversity of Nebraska Medical CenterOmahaNebraskaUSA 68198-4388 Department of Radiation Chemistry and Toxicology School of Public HealthJilin UniversityChangchun 130021JilinChina

Globally,breast cancer is the second major cause of cancer death among women[1].Cyclophosphamide(CTX)is a common chemotherapeutic agent for patients with breast cancer who have undergone *** metabolites act by disrupting deoxyribonucleic acid and by arresting the cell cycle of tumor cells,which results in their apoptosis or growth inhibition[2].However,CTX triggers the production of excessive amounts of reactive oxygen species(ROS),which produces various side effects in different organs or systems[3].In the initial stage of cancer development,ROS may promote the expansion of cancerous cells by modifying apoptosis-related genes[4].Furthermore,ROS will activate the NF-κB pathway and NF-κB-dependent genes that are involved in apoptosis and cell adhesion,which are implicated in multiple aspects of cancer development[5].ROS,as a survival or apoptotic signal to cancer cells,is determined by the dosage,duration,type,and site of ROS production[6].

关键词： dosage organs cancer

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Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication

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World Journal of Gastroenterology 2015年第32期21卷 9554-9565页

作者： Jie Wang Zhong-Wei Xu Shuang Liu Rui-Yang Zhang Shan-Long Ding xiao-Meng Xie Lu Long Xiang-mei Chen Hui Zhuang Feng-Min Lu State Key Laboratory of Natural and Biomimetic Drugs Department of Microbiology and Infectious Disease Center School of Basic Medicine Peking University Health Science Center Department of Gastroenterology Pennsylvania Hospital University of Pennsylvania Beijing Artificial Liver Treatment and Training Center Beijing Youan Hospital Capital Medical University Department of Epidemiology and Biostatistics College of Public Health Zhengzhou University

AIM: To screen and investigate the effective g RNAs against hepatitis B virus(HBV) of genotypes ***: A total of 15 g RNAs against HBV of genotypes A-D were designed. Eleven combinations of two above g RNAs(dual-g RNAs) covering the regulatory region of HBV were chosen. The efficiency of each g RNA and 11 dual-g RNAs on the suppression of HBV(genotypes A-D) replication was examined by the measurement of HBV surface antigen(HBs Ag) or e antigen(HBe Ag) in the culture supernatant. The destruction of HBV-expressing vector was examined in Hu H7 cells co-transfected with dual-g RNAs and HBVexpressing vector using polymerase chain reaction(PCR) and sequencing method, and the destruction of ccc DNAwas examined in Hep AD38 cells using KCl precipitation, plasmid-safe ATP-dependent DNase(PSAD) digestion, rolling circle amplification and quantitative PCR combined method. The cytotoxicity of these g RNAs was assessed by a mitochondrial tetrazolium ***: All of g RNAs could significantly reduce HBs Ag or HBe Ag production in the culture supernatant, which was dependent on the region in which g RNA against. All of dual g RNAs could efficiently suppress HBs Ag and/or HBe Ag production for HBV of genotypes A-D, and the efficacy of dual g RNAs in suppressing HBs Ag and/or HBe Ag production was significantly increased when compared to the single g RNA used alone. Furthermore, by PCR direct sequencing we confirmed that these dual g RNAs could specifically destroy HBV expressing template by removing the fragment between the cleavage sites of the two used g RNAs. Most importantly, g RNA-5 and g RNA-12 combination not only could efficiently suppressing HBs Ag and/or HBe Ag production, but also destroy the ccc DNA reservoirs in Hep AD38 ***: These results suggested that CRISPR/Cas9 system could efficiently destroy HBV expressing templates(genotypes A-D) without apparent cytotoxicity. It may be a potential approach for eradication of persistent HBV ccc DNA in c

关键词： Dual g RNAs CRISPR/Cas9 Hepatitis B ccc DNA Antivi

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Crystal-phase engineering of PdCu nanoalloys facilitates selective hydrodeoxygenation at room temperature

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The Innovation 2022年第1期3卷 31-37页

作者： Shaopeng Li Minghua Dong Mi Peng Qingqing mei Yanyan Wang Junjuan Yang Youdi Yang Bingfeng Chen Shulin Liu Dequan xiao Huizhen Liu Ding Ma Buxing Han Beijing National Laboratory for Molecular Sciences Key Laboratory of Colloid and Interface and ThermodynamicsCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China School of Chemical Science University of Chinese Academy of SciencesBeijing 100049China Beijing National Laboratory for Molecular Engineering College of Chemistry and Molecular Engineering and College of EngineeringBIC-ESATPeking UniversityBeijing 100871China Center for Integrative Materials Discovery Department of Chemistry and Chemical EngineeringUniversity of New HavenWest HavenCT 06516USA

Selective hydrodeoxygenation of biomass-derived aromatic alcohols to value-added chemical or fuel is of great importance for sustainable biomass upgrading,and hydrodeoxygenation of 5-hydroxymethylfurfural(HMF)to 2,5-dimethylfuran(DMF)is one of the most attractive *** the conversion of HMF to DMF using H_(2)at ambient temperature is *** this work,we used PdCu nanoalloys to catalyze the selective hydrodeoxygenation reaction of HMF to DMF using H_(2)as the reducing *** reaction path and the product selectivity are governed by the crystallographic phase of the PdCu *** was discovered that body-centered cubic(BCC)PdCu nanoalloys supported on activated carbon(AC)exhibited outstanding performance with 93.6%yield of DMF at room temperature(PdCu/AC-BCC).A combination of experimental and density functional theory(DFT)studies showed that the tilted adsorption modes of furanic intermediates on PdCu-BCC nanoalloy surfaces accounted for the high selectivity of DMF;however,furan ring was activated on PdCu face-centered cubic(FCC)nanoalloy ***,PdCu/AC-BCC could also catalyze the hydrodeoxygenation of other aromatic alcohols at room temperature while maintaining the aromatic *** work opens the way for selective hydrodeoxygenation of the aromatic alcohols at room temperature with the aromatic ring intact.

关键词： alcohols aromatic selectivity

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Evaluation and Application of Two High-Iron Transgenic Rice Lines Expressing a Pea Ferritin Gene

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Rice science 2008年第1期15卷 51-56页

作者： YE Hong-xia LI mei Guo Ze-jian SHU Qing-yao Xu xiao-hui BAO Jin-song SHEN Sheng-quan College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Department of Plant Pathology China Agricultural University Beijing 100094 China College of Life Science China Jiliang University Hangzhou 310018China

A total of 105 transgenic dce lines independently transformed with a pea ferritin gene （Fer） were previously obtained. After seven generations of selfing and β-glucuronidase （GUS） assisted selection, 82 transgenic lines with stable agronomic traits were got. Among the 82 transgenic lines, two high-iron transgenic rice lines Fer34 and Fer65, with the iron contents in the milled rice being 4.82 and 3.46 times of that of the wild type Xiushui 11, respectively were identified. In the two transgenic lines, the exogenous Fer gene was highly expressed, and inherited as a single locus. The transgene had no negative effect on the agronomic traits of rice plant, other mineral nutritional components, appearance quality and eating quality of the milled rice, indicating that these two lines were elite high-iron breeding lines. Furthermore, the practical application and further studies facilitating utilization of the two elite breeding lines were discussed.

关键词： transgenic rice ferritin gene iron mineral nutrition rice quality

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Dietary Behaviors and Influencing Factors among Rural Left-behind Children Aged below 7 Years in China

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Biomedical and Environmental Sciences 2018年第12期31卷 902-907页

作者： FAN Chun Li MA Quan Fu LUO Jia You WU Xu Feng LUO Mi Yang ZENG Rong LI xiao Hui LI Ya mei FANG Jun Qun Department of Gynecology Maternal and Child Health Hospital of Hubei Province Wuhan 430070 Hubei China Central South University Changsha 410000 Hunan China Department of Epidemiology Saw Swee Hock School of Public Health National University of Singapore119077 Singapore Xiangya Hospital of Central South University Changsha 410000 Hunan China Department of Child Health Care Maternal and Child Health Hospital of Hunan Provincial Changsha 410000 Hunan China

Left-behind children comprise a special population inChinese rural areas. According to the 2007 China Census,the number ofleft-behind children aged≤14 years was estimated at 58 million which suggested that these chil... 详细信息

关键词： Dietary Behaviors and Influencing Factors among Rural Left-behind Children Aged below 7 Years in China

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