检索结果-南通市图书馆

Characterization of long-term ex vivo expansion of tree shrew spermatogonial stem cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Zoological Research 2023年第6期44卷 1080-1094页

作者： Cong Li Rui Bi Lin Wang Yu-Hua Ma yong-Gang yao Ping Zheng State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Key Laboratory of Animal Models and Human Disease Mechanisms of Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan 650204China National Resource Center for Non-Human Primates National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650107China KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650204China

Tree shrews(Tupaia belangeri chinensis)share a close relationship to primates and have been widely used in biomedical *** previously established a spermatogonial stem cell(SSC)-based gene editing platform to generate transgenic tree ***,the influences of long-term expansion on tree shrew SSC spermatogenesis potential remain ***,we examined the in vivo spermatogenesis potential of tree shrew SSCs cultured across different *** found that SSCs lost spermatogenesis ability after long-term expansion(>50 passages),as indicated by the failure to colonize the seminiferous epithelium and generate donor spermatogonia(SPG)-derivedspermatocytesor spermatids marking *** sequencing(RNA-seq)analysis of undifferentiated SPGs across different passages revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages on feeder ***,DNA damage response and repair genes(e.g.,MRE11,SMC3,BLM,and GEN1)were down-regulated,whereas genes associated with mitochondrial function(e.g.,NDUFA9,NDUFA8,NDUFA13,and NDUFB8)were up-regulated after *** DNA damage accumulation and mitochondrial dysfunction were experimentally validated in high-passage *** with nicotinamide adenine dinucleotide(NAD+)precursor nicotinamide riboside(NR)exhibited beneficial effects by reducing DNA damage accumulation and mitochondrial dysfunction in SPG elicited by long-term *** research presents a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.

关键词： Tree shrews Spermatogonial stem cells Nicotinamide riboside DNA damage Mitochondrial dysfunction

Site testing campaign for the Large Optical Telescope at the Ali site

在线全文

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2020年第6期20卷 141-150页

作者： Li-yong Liu yong-Qiang yao Jia Yin Hong-Shuai Wang Jun-Rong Li Yun-He Zhou Xian-Long You Peng Tang Xi-Yue Zhao De-Qiang Ma Jian Dong National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

The Large Optical/infrared Telescope(LOT)is a ground-based 12 m diameter telescope which is proposed to be built in western *** site selection for LOT in China began in 2016,and Ali was listed as one of the three candidate *** studies and local surveys have been carried out for more than 15 years in western China,and the results show that Ali is a promising site with comprehensive quality in terms of atmospheric and supporting *** overview of the site testing campaign at the Ali site from 2016 to 2019 is *** the two years of data collection,the overall median seeing value is found to be 1.17 arcsec,the observable nights are 81.71%and the good observable nights are 71.76%.The weather conditions as follows,the median night temperature value is-5.18℃,the median night relative humidity value is 41.25%,the median night atmospheric pressure value is 540.92 hPa,the median night wind speed value is 7.41 m s-1 and the mainly wind direction is southwestern(SW).The median night sky background value is 22.07 *** also discuss the wind speed at different locations on-site,the possibility of light pollution and the effect of wind speed on differential image motion monitor(DIMM)seeing measurements.

关键词： large optical telescope site testing Ali site cloudiness seeing

Recent development of pillar[n]arene-based amphiphiles

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2021年第4期32卷 1267-1279页

作者： Yan Cai Zhecheng Zhang Yue Ding Lanping Hu Jin Wang Tingting Chen yong yao* School of Chemistry and Chemical Engineering Nantong UniversityNantong 226019China

Pillar[n]arene-based amphiphiles,mainly including amphiphilic pillar[n]arenes and supra-amphiphilic pillar[n]arenes,have obtained considerable interests in recent years due to their fascinating chemical structures,various self-assembly behaviors,and widely *** to the pillar-like frameworks and the rich host-guest recognitions of the cavities,these amphiphiles can be easily controlled to form dimensional and morphologic assemblies for multiple *** with traditional linear covalent amphiphiles,the introduction of host-guest recognitions facilitated the preparation and controllability of these supramolecular amphiphilic ***,the host-guest recognitions endow the assemblies from pillar[n]arene-based amphiphiles with stimuli-responsive *** this mini-review,we summarized the chemical structures,self-assembly features,and the applications of pillar[n]arene-based ***,several research topics of pillar[n]arenebased amphiphiles can be further developed in the future,such as larger cavity amphiphilic pillar[n]arenes,co-assembly with 2 D materials and utilization of the host-guest interactions.

关键词： Pillararene Supramolecular assemblies Amphiphiles Reversible conversion Controlled release

Joining of C_f/SiBCN composite with CuPd-V filler alloy

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2018年第9期34卷 1652-1659页

作者： Wen-Wen Li Bo Chen La-Mei Cao Wei Liu Hua-Ping Xiong yao-yong Cheng Welding and Plastic Forming Division Beijing Institute of Aeronautical Materials Beijing 100095 China Science and Technology on Advanced High Temperature Structural Materials taboratory Beijing Institute of Aeronautical Materials Beijing 100095 China

Two compositions of CuPd-V system filler alloy were designed for joining the Cf/SiBCN composite. Their dynamic wettability on the Cf/SiBCN composite was studied with the sessile drop method. The CuPd-8 V alloy exhibited a contact angle of 57° after holding at 1170℃ for 30 min, whereas for CuPd-13 V alloy,a lower contact angle of 28°can be achieved after heating at 1200 ℃ for 20 min. Sound Cf/SiBCN joints were successfully produced using the latter filler alloy under the brazing condition of（1170-1230）℃for 10 min. The results showed that the active element V strongly diffused to the surface of Cf/SiBCN composite, with the formation of V2 C/VN reaction layer. The microstructure in the central part of the joint brazed at 1200 ℃ was characterized by the V2 C/VN particles distributing scatteringly in CuPd matrix. The corresponding joints showed the maximum three-point bend strength of 82.4 MPa at room *** the testing temperature was increased to 600 0 C, the joint strength was even elevated to 108.8 ***, the joints exhibited the strength of 92.4 MPa and 39.8 MPa at 800 ℃ and 900 ℃, respectively.

关键词： Cf/SiBCN composite Wettability Joint strength Interfacial reaction

Immobilized chitosan as a selective absorbent for the nickel removal in water sample

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2003年第5期15卷 633-638页

作者： WU Jian min, WANG yong yao* (Department of Chemistry, College of Science, Zhejiang University, Hangzhou 310027, China. ) Department of Chemistry College of Science Zhejiang University Hangzhou 310027 Chin

Method for preparation of chitosan immobilized on silica gel(CTS silica) was described. The CTS silica was used as absorbent for the absorption of nickel in water. The results showed that this absorbent had relatively high selectivity and strong affinity to nickel. The maximum absorption capacity for nickel can reach 667 mg/g of chitosan. Factors that affect the absorption capacity, such as pH, ion strength and the presence of calcium, EDTA and the mechanism of absorption were discussed in detail. The absorbent can be regenerated with acid and reused for several times. The recovery rate for nickel can reach 99 99%. This absorbent filled in a column can be used in nickel removal from wastewater and drinking water.

关键词： immobilized chitosan nickel wastewater treatment

Corrosion Behaviour of a Non‑equiatomic CoCrFeNiMo High‑Entropy Alloy in H_(2)S‑Containing and H_(2)S‑Free Environments

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2023年第3期36卷 366-378页

作者： Zhu Wang Guo‑Hui Zhang yong yao* Xue‑Hua Fan Jie Jin Lei Zhang Yan‑Xia Du Institute for Advanced Materials and Technology University of Science and Technology BeijingBeijing 100083China CITIC Pacific Special Steel Group Co. LtdJiangsu 214429China China Petroleum Engineering&Construction Co. Ltd Beijing CompanyBeijing 100085China

The corrosion behaviour of a non-equiatomic CoCrFeNiMo high-entropy alloy(HEA)in H_(2)S-containing and H_(2)S-free environments was studied by electrochemical tests,surface characterization,and solution *** results showed that the HEA exhibited primary and secondary passivation in the H_(2)S-free environment,and the transition was owing to the enhanced dissolution of Fe *** with the primary passive film,the Cr/Fe ratio in the secondary passive film increased at the expense of the selective dissolution of ***,the corrosion resistance of HEA decreased with the applied *** was the most stable element in the film of HEA,regardless of H_(2)*** presence of H_(2)S accelerated the dissolution of all the cationic elements in the HEA.H_(2)S promoted the formation of thicker but less protective surface film and induced the loss of passivity.

关键词： High-entropy alloy(HEA) Corrosion H_(2)S Secondary passivation X-ray photoelectron spectroscopy(XPS) Induced coupled plasma(ICP)

A Practical Approach to Synthesize Multi-channel Fiber Bragg Grating with Right-Angled Triangular Spectrum

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics Letters 2012年第11期29卷 98-101页

作者： YU Xue-Lian yao yong XIAO Jun-Jun TIAN Jia-Jun School of Electronic and Information Engineering Shenzhen Graduate SchoolHarbin Institute of TechnologyShenzhen 518055 Department of Optics Information Science and Technology Harbin University of Science and TechnologyHarbin 150080

A novel approach is proposed towards the design of fiber Bragg gratings with multi-channel right-angled triangular ***,a single-channel grating is synthesized utilizing an adaptive quantum particle swarm optimization with the piecewise constant mutated ***,the reflectivity spectrum with good linear edge for a short grating is ***,for its merits of easy fabrication,the superposition method is adopted to design multi-channel gratings with initial spectral ***,this distortion is optimized by the method in the first *** is shown that the design outcomes still retain the features of easy fabrication and short *** gratings would be useful as wavelength-interrogation devices with multiple physical parameters in optical sensor systems.

关键词： channel Angle utilizing

SCSMRD: A database for single-cell skeletal muscle regeneration

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第3期22卷 864-871页

作者： FENG Xi-kang XIE Chun-di LI yong-yao WANG Zi-shuai BAI Li-jing School of Software Northwestern Polytechnical UniversityXi’an 710072P.R.China Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000P.R.China

Skeletal muscle regeneration is a complex process where various cell types and cytokines are ***-cell RNA-sequencing (scRNA-seq) provides the opportunity to deconvolute heterogeneous tissue into individual cells based on their transcriptomic *** scRNA-seq studies on mouse muscle regeneration have provided insights to understand the transcriptional dynamics that underpin muscle ***,a database to investigate gene expression profiling during skeletal muscle regeneration at the single-cell level is ***,we collected over 105 000 cells at 7 key regenerative time-points and non-injured muscles and developed a database,the Singlecell Skeletal Muscle Regeneration Database (SCSMRD).SCSMRD allows users to search the dynamic expression profiles of genes of interest across different cell types during the skeletal muscle regeneration *** also provides a network to show the activity of regulons in different cell types at different time *** analysis showed the state changes trajectory of muscle stem cells (MuSCs) during skeletal muscle *** database is freely available at https://***.

关键词： scRNA-seq skeletal muscle regeneration database regulon network pseudotime

一类半正定多项式的平方和分解及其表达式的自动生成

在线全文

学校读者我要写书评

暂无评论

计算机学报 2006年第10期29卷 1862-1868页

作者：姚勇冯勇中国科学院成都计算机应用研究所成都610041

建立了一个把半正定稀疏多项式表为多项式平方和的算法.这一算法依赖于Hilbert第17问题的一系列经典研究结果以及实闭域上量词消去的柱形代数剖分算法.该算法的机器实现为一类代数不等式可读性证明的自动生成提供了一种非常自然的途径.

关键词：平方和分解柱形代数剖分 Gram矩阵方法 Hilbert 17问题