检索结果-南通市图书馆

Upregulation of miR-34c after silencing E2F transcription factor 1 inhibits paclitaxel combined with cisplatin resistance in gastric cancer cells

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2020年第5期26卷 499-513页

作者： Hong Zheng Jin-Jing Wang Xiao-rong yang yong-Lin Yu Department of Pathology the Affiliated Hospital of Zunyi Medical University

BACKGROUND MicroRNA 34c(miR-34c)has been reported to be associated with malignant types of cancer,however,it remains unknown whether miR-34c is involved in chemoresistance in gastric cancer(GC).AIM To investigate the effect of miR-34c and its upstream transcription factor E2F1 on paclitaxel combined with cisplatin resistance in GC *** Paired GC tissues and adjacent normal tissues were randomly sampled from 74 GC ***-34c and E2F1 were detected by real-time quantitative PCR(qPCR)and Western *** addition,the drug resistance of GC cells to paclitaxel and cisplatin was induced by concentration gradient increasing methods,and changes in miR-34c and E2F1 during this process were ***,E2F1 and miR-34c overexpression or underexpression vectors were constructed and transfected into drug-resistant GC *** was employed to test the sensitivity of cells to paclitaxel combined with cisplatin,qPCR was adopted to detect the expression of miR-34c,Western blot was applied to detect the expression levels of E2F1,drug resistance-related proteins and apoptosis-related proteins,and flow cytometry was used for the determination of cell apoptosis and cell cycle *** E2F1 was overexpressed while miR-34c was underexpressed in *** inducing GC cells to be resistant to paclitaxel and cisplatin,E2F1 expression increased while miR-34c expression *** silencing E2F1 and overexpressing miR-34c could increase the sensitivity of drug-resistant GC cells to paclitaxel combined with cisplatin,promote cell apoptosis and inhibit cell *** which,silencing E2F1 could reduce the expression of drug resistance-related proteins and apoptosis-related proteins,while over-expression of miR-34c could upregulate the expression of apoptosis-related proteins without affecting the expression of MDR-1,MRP and other drug resistance-related *** experiments demonstrated that inhibiting miR-34c could significantly weaken the sensi

关键词： E2F transcription factor 1 MicroRNA 34c Gastric cancer Paclitaxel combined with cisplatin resistance

SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2015年第2期14卷 326-336页

作者： SUN Xiao-chun GAO yong-feng ZHANG Ning LI Hui-rong yang Shu-zhang LIU yong-sheng Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment/State Key Laboratory of Hydraulics and Mountain River Engineering/College of Life Science Sichuan University School of Biotechnology and Food Engineering Hefei University of Technology

SOM encodes a nucleus-localized CCCH-type zinc finger protein and negatively regulates seed germination in Arabidopsis thaliana. We have previously demonstrated that ectopic expression of Sl ABI3, an important transcription factor in abscisic acid（ABA） signaling pathway, resulted in alteration of Sl SOM expression patterns in both leaf and seed of tomato. In this study, we aimed to elucidate the function of tomato Sl SOM in regarding to seed germination and seedling development. Here, we constructed Sl SOM over-expression vector p BI121-SOM driven by Ca MV 35 S promoter, and the recombinant plasmid was incorporated into wild-type tomato by the method of Agrobacterium tumefaciens-mediated transformation. The result showed that over-expression of Sl SOM conferred enhanced responses to exogenous ABA application during seed germination and seedling development. In addition, ectopic expression of Sl SOM resulted in the alteration of expression level of ABA/GA（gibberellins） metabolic genes, such as Sl ABA1, Sl CYP707A1, Sl GA3ox2, and Sl GA2ox4, in both leaf and seed. The ABA anabolic gene Sl ABA1 and the GA catabolic gene Sl GA2ox4 were up-regulated while the ABA catabolic gene Sl CYP707A1 and the GA anabolic gene Sl GA3ox2 were down-regulated. Compared to wild type, the expression level of Sl ABI5 was increased by about 40–50% in transgenic seeds while adding exogenous ABA treatment. These results support the notion that Sl SOM inhibits seed germination by regulating ABA/GA metabolic genes and Sl ABI5 expression in Solanum lycopersicum.

关键词： tomato SOM seed germination abscisic acid

Machine learning for prediction of retained austenite fraction and optimization of processing in quenched and partitioned steels

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2024年第8期31卷 2002-2013页

作者： Shuai Wang Jie Li Li-yang Zeng Xun-wei Zuo Nai-lu Chen yong-hua rong Shanghai Key Laboratory of Materials Laser Processing and Modification School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China

The metastable retained austenite(RA)plays a significant role in the excellent mechanical performance of quenching and partitioning(Q&P)steels,while the volume fraction of RA(V_(RA))is challengeable to directly predict due to the complicated relationships between the chemical composition and process(like quenching temperature(Qr)).A Gaussian process regression model in machine learning was developed to predict V_(RA),and the model accuracy was further improved by introducing a metallurgical parameter of martensite fraction(fo)to accurately predict V_(RA) in Q&P *** developed machine learning model combined with Bayesian global optimization can serve as another selection strategy for the quenching temperature,and this strategy is very effcient as it found the"optimum"Qr with the maximum V_(RA) using only seven consecutive *** benchmark experiment also reveals that the developed machine learning model predicts V_(RA) more accurately than the popular constrained carbon equilibrium thermodynamic model,even better than a thermo-kinetic quenching-partitioning-tempering-local equilibrium model.

关键词： Q&P steel Retained austenite fraction Machine learning Quenching temperature Gaussian process regression model

Quantile Regression for Right-Censored and Length-Biased Data

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Mathematicae Applicatae Sinica 2012年第3期28卷 443-462页

作者： Xue-rong CHEN yong ZHOU Department of Statistics Yunnan UniversityKunming 650091China Academy of Mathematics and System Sciences Chinese Academy of ScienceBeijing 100190China School of Statistics and management Shanghai University of Finance and EconomicsShanghai 200433

Length-biased data arise in many important fields, including epidemiological cohort studies, cancer screening trials and labor economics. Analysis of such data has attracted much attention in the literature. In this paper we propose a quantile regression approach for analyzing right-censored and length-biased data. We derive an inverse probability weighted estimating equation corresponding to the quantile regression to correct the bias due to length-bias sampling and informative censoring. This method can easily handle informative censoring induced by length-biased sampling. This is an appealing feature of our proposed method since it is generally difficult to obtain unbiased estimates of risk factors in the presence of length-bias and informative censoring. We establish the consistency and asymptotic distribution of the proposed estimator using empirical process techniques. A resampling method is adopted to estimate the variance of the estimator. We conduct simulation studies to evaluate its finite sample performance and use a real data set to illustrate the application of the proposed method.

关键词： length-biased sampling right-censored information censoring quantile regression estimatingequations resampling method

Coupled dynamic response analysis of multi-column floating offshore wind turbine with low center of gravity

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean Engineering and Science 2024年第1期9卷 25-39页

作者： Jie yang Yan-ping He yong-sheng Zhao Xiao-yan yang Guo-rong Zhang State Key Laboratory of Ocean Engineering Shanghai Jiao Tong UniversityShanghai 200240China Collaborative Innovation Center for Advanced Ship and Deep-Sea Exploration(CISSE) Shanghai Jiao Tong UniversityShanghai 200240China School of Naval Architecture Ocean&Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China Faculty of Maritime and Transportation Ningbo UniversityNingbo 315211China

To realize the application of the floating offshore wind turbine(FOWT)from deep to relatively shallow waters,a new concept of multi-column floating wind turbine platform with low center of gravity(CG)is designed and *** multi-column low CG platform is designed to support a 6MW wind turbine class and operated at a water depth of 50m in the South China *** frequency domain software WADAM and time domain software NREL-FAST are used to simulate coupled dynamic responses of the floating wind turbine system with second-order wave loads *** dynamic behaviors of multi-column low CG FOWT system under normal operation and parked conditions are *** influence of second-order wave force on the motion responses of the multi-column platform,fore-aft force and moment of the tower base and mooring force are researched *** results demonstrate that the coupled dynamic responses at rated operating condition and extreme condition meet the normal operating requirements and extreme survival requirements of FOWT system in the shallow water(50m)of South China *** addition,it is found that,the wave frequency response gradually replaces the second-order low frequency response as the main influencing factor of the coupled dynamic response of the FOWT system with the increasing severity of the sea ***,in general,the magnitude of second-order low frequency response increases with the increasing severity of the design load ***,in the subsequent design of the shallow water FOWT system,the second-order effects should be paid enough attention.

关键词： Floating offshore wind turbine Multi-column platform Second-order wave force Concept design Coupled dynamic response

Influence of the embedded structure on the EFP formation of compact terminal sensitive projectile

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Defence Technology（防务技术） 2017年第4期13卷 310-315页

作者： Bo-yang Xing rong-zhong Liu Rui Guo Liang Chen Hao Zhou yong-liang yang Lei Liu School of Mechanical Engineering Nanjing University of Science and Technology Faculty of Engineering University of Nottingham Department of Mechanical Engineering Eindhoven University of Technology

To improve the damage efficiency of compact terminal sensitive projectile with EFP warhead,it is vital to understand how the embedded structure(ES)affects the EFP forming *** this paper,the corresponding numerical investigation is focused on,in which the fluid-structure interaction(FSI)method and the experimental verification are *** on the obtained quantitative relations between the forming performance and a(the ratio of height to maximum radius of ES),an optimal design is further *** results indicate that:when the embedded structural length and width range 0.1e0.3D and 0.1e0.2D(D:diameter of EFP warhead)at a fixed volume,respectively,EFP forming velocity nearly keeps as a constant,1760 m/s;the height of ES has a dramatical effect on the propagating range of detonation wave,resulting in significant influence on the aerodynamic shape and length-to-diameter ratio of EFP;under the given constraints,the EFP length-diameter ratio can reach the optimal value2.76,when the height of ES is 0.22D.

关键词： Terminal sensitive projectile Embedded structure Detonation wave EFP formation

Unfolding analysis of LaBr3:Ce gamma spectrum with a detector response matrix constructing algorithm based on energy resolution calibration

在线全文

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 2018年第1期29卷 23-31页

作者： Rui Shi Xian-Guo Tuo Huai-Liang Li yang-yang Xu Fan-rong Shi Jian-Bo yang yong Luo Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory Southwest University of Science and Technology Mianyang 621010 China Sichuan University of Science and Engineering Zigong 643000 China State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology Chengdu 610059 China

With respect to the gamma spectrum, the energy resolution improves with increase in energy. The counts of full energy peak change with energy, and this approximately complies with the Gaussian distribution. This study mainly examines a method to deconvolve the LaBr_3:Ce gamma spectrum with a detector response matrix constructing algorithm based on energy resolution *** the algorithm, the full width at half maximum(FWHM)of full energy peak was calculated by the cubic spline interpolation algorithm and calibrated by a square root of a quadratic function that changes with the energy. Additionally, the detector response matrix was constructed to deconvolve the gamma spectrum. Furthermore, an improved SNIP algorithm was proposed to eliminate the background. In the experiment, several independent peaks of ^(152)Eu,^(137)Cs, and ^(60)Co sources were detected by a LaBr_3:Ce scintillator that were selected to calibrate the energy resolution. The Boosted Gold algorithm was applied to deconvolve the gamma spectrum. The results showed that the peak position difference between the experiment and the deconvolution was within ± 2 channels and the relative error of peak area was approximately within 0.96–6.74%. Finally, a ^(133) Ba spectrum was deconvolved to verify the efficiency and accuracy of the algorithm in unfolding the overlapped peaks.

关键词： Detector response matrix Energy resolution calibration LaBr3:Ce scintillator SNIP background elimination Boosted Gold deconvolution algorithm

Gaussian mixture model based adaptive control for uncertain nonlinear systems with complex state constraints

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Aeronautics 2022年第5期35卷 361-373页

作者： Yuzhu BAI rong CHEN yong ZHAO Yi WANG College of Aerospace and Engineering National University of Defense TechnologyChangsha 410073China Nanjing Research Institute of Electronics Technology Nanjing 210000China

This paper addresses an uncertain nonlinear control system problem with complex state constraints and mismatched uncertainties.A novel Gaussian Mixture Model(GMM)based adaptive PID-Nonsingular Terminal Sliding Mode Control(NTSMC)(GMM-adaptive-PID-NTSMC)method is *** is achieved by combining a GMM based adaptive potential function with a novel switching surface of ***,the stability of the closed-loop system is *** main contribution of this paper is that the GMM method is applied to obtain the analytic description of the complex bounded state constraints,ensuring that the states'constraints are not violated with GMM-based adaptive potential *** developed potential function can consider the influence of *** importantly,the GMM-adaptive-PID-NTSMC can be generalized to control a more representative class of uncertain nonlinear systems with constrained states and mismatched *** addition,the proposed controller enhances the robustness,and requires less control cost and reduces the steady state error with respect to the Artificial Potential Function based Nonsingular Terminal Sliding Mode Control(APF-NTSMC),GMM-NTSMC and *** last,numerical simulation is performed to validate the superior performance of the proposed controller.

关键词： Gaussian mixture model Nonlinear systems State constrained Terminal sliding mode control Uncertain

Effects of topography on dynamic responses of single piles under vertical cyclic loading

在线全文

学校读者我要写书评

暂无评论

Journal of Mountain Science 2020年第1期17卷 230-243页

作者： QU Li-ming DING Xuan-ming WU Chong-rong LONG yong-hong yang Jin-chuan College of Civil Engineering Chongqing UniversityChongqing 400045China Key Laboratory of New Technology for Construction of Cities in Mountain Area Chongqing UniversityChongqing 400045China Citation:Qu LMDing

This paper describes model tests of single piles subjected to vertical cyclic compressive loading for three kinds of topography: sloping ground, level ground, and inclined bedrock. Comprehensive dynamic responses involving cyclic effects and vibration behaviours are studied under various load combinations of dynamic amplitude, mean load,frequency and number of cycles. Test results show that permanent settlement can generally be predicted with a quadratic function or power function of *** ground topography produces more pronounced settlement than level ground under the same load condition. For vibration behaviour,displacement amplitude is weakly affected by the number of cycles, while load amplitude significantly influences dynamic responses. Test results also reveal that increasing load amplitude intensifies nonlinearity and topography effects. The strain distribution in a pile and soil stress at the pile tip are displayed to investigate the vibration mechanism accounting for sloping ground effects. Furthermore, the dynamic characteristics among three kinds of topography in the elastic stage are studied using a three-dimensional finite method. Numerical results are validated by comparing with experimental results for base inclination topography. An inclined soil profile boundary causes non-axisymmetric resultant deformation, though a small difference in vertical displacement is observed.

关键词： Pile Model test Dynamic response Permanent settlement Vibration displacement Topography effects