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Molecular genome tracking of East,Central and South African genotype of Chikungunya virus in South-east Asia between 2006 and 2009

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Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 535-540页

作者： Kamol Suwannakarn Apiradee Theamboonlers yong poovorawan Center of Excellence in Clinical Virology Faculty of MedicineChulalongkorn University and King Chulalongkorn Memorial HospitalBangkokThailand

Objective:To understand the epidemiology of the East,Central and South African(ECSA) genotype of Chikungunya virus(CHIKV) in terms of emerging and re-emerging infections,this study has been aimed at investigating the evolutionary parameters,genomic signatures and molecular tracking of the CHIKV ECSA genotype in South-east Asia and coastal areas of the Indian Ocean between 2006 and 2009 by using phylogenetie analysis and the Bayesian Markov Chain Monte Carlo(BMCMC) evolutionary ***:Nearly complete genome sequences of 53 CHIKV isolates from all genotypes were subjected to phylogenetie analysis and evolutionary parameter *** amino acids of 67 of ECSA genotype during 2006 to 2009 were compared for finding molecular signature *** ECSA genotype signatures were visualized to find the possible transmission root was projected onto a geographic ***: Phylogenetie analysis showed the ECSA genotype was divided into 2 *** first group comprises viruses from India and Southeast Asian *** second group consists of strains typically circulating in Sri Lanka in *** evolutionary parameters of these groups depicted the time of the most recent common ancestor at approximately 7.5 years *** genomic signatures revealed the positions of amino acid variation in each ***:The molecular evolution projected onto a geographical map showed the routes of CHIKV transmission from 2006 to *** tracking will assist in understanding transmission routes, epidemiology and molecular evolution of CHIKV.

关键词： Chikungunya virus East Central and South African genotype Genomic signature Evolutionary parameter

Hepatitis C virus genotype 6:Virology,epidemiology,genetic variation and clinical implication

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World Journal of Gastroenterology 2014年第11期20卷 2927-2940,1812页

作者： Vo Duy Thong Srunthron Akkarathamrongsin Kittiyod poovorawan Pisit Tangkijvanich yong poovorawan Center of Excellence in Clinical Virology Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand Department of Medicine Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand Department of Biochemistry Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand

Hepatitis C virus (HCV) is a serious public health problem affecting 170 million carriers worldwide. It is a leading cause of chronic hepatitis, cirrhosis, and liver cancer and is the primary cause for liver transplantation worldwide. HCV genotype 6 (HCV-6) is restricted to South China, South-East Asia, and it is also occasionally found in migrant patients from endemic countries. HCV-6 has considerable genetic diversity with 23 subtypes (a to w). Although direct sequencing followed by phylogenetic analysis is the gold standard for HCV-6 genotyping and subtyping, there are also now rapid genotyping tests available such as the reverse hybridization line probe assay (INNO-LiPA II; Innogenetics, Zwijnaarde, Belgium). HCV-6 patients present with similar clinical manifestations as patients infected with other genotypes. Based on current evidence, the optimal treatment duration of HCV-6 with pegylated interferon/ribavirin should be 48 wk, although a shortened treatment duration of 24 wk could be sufficient in patients with low pretreatment viral load who achieve rapid virological response. In addition, the development of direct-acting antiviral agents is ongoing, and they give high response rate when combined with standard therapy. Herein, we review the epidemiology, classification, diagnosis and treatment as it pertain to HCV-6.

关键词： Hepatitis C virus Genotype 6 Epidemiology Clinical Treatment

IL-18 and IL-18 binding protein levels in patients with dengue virus infection

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Asian Pacific Journal of Tropical Medicine 2010年第10期3卷 812-814页

作者： Chintana Chirathaworn yong poovorawan Viboonsak Vuthitanachot Department of Microbiology Faculty of MedicineChulalongkorn University Department of Pediatrics Faculty of MedicineChulalongkorn University Chumphae Hospital

Objective:To determine the levels of IL-18 and IL-18 binding protein(BP) in patients with dengue virus ***:Acute and convalescent sera were collected from each patient. Control group was sera from blood *** levels of both IL-18 and IL-18BP were measured by ELISA ***:It was shown that IL-18 and IL-18BP levels were significantly higher in patients when compared with *** addition,the level of IL-18BP was lower in convalescent than in acute ***:These data suggest that both IL-18 and IL-18BP production was induced following dengue virus *** the regulation of IL-18 by its natural regulator could lead to further understanding of the immune response or immunopathogenesis following dengue virus infection.

关键词： Dengue virus IL-18 IL-18BP

Immunization status and hospitalization for vaccine-preventable and non-vaccine-preventable infections in liver-transplanted children

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World Journal of Hepatology 2021年第1期13卷 120-131页

作者： Palittiya Sintusek yong poovorawan Thai Pediatric Gastroenterology Hepatology and Immunology Research UnitFaculty of MedicineChulalongkorn UniversityBangkok 10330Thailand Department of Pediatrics Faculty of MedicineKing Chulalongkorn Memorial HospitalChulalongkorn UniversityBangkok 10330Thailand Center of Excellence in Clinical Virology Department of PediatricsFaculty of MedicineKing Chulalongkorn Memorial HospitalChulalongkorn UniversityBangkok 10330Thailand

BACKGROUND Infections and associated morbidity and mortality may be more frequent in children who have undergone liver transplant than in healthy *** strategies to prevent vaccine-preventable infections(VPIs)can effectively minimize this infection ***,data on age-appropriate immunization and VPIs in children after liver transplant in Asia are *** To evaluate the immunization status,VPIs and non-VPIs requiring hospitalization in children who have undergone a liver *** The medical records of children who had a liver transplant between 2004 and 2018 at King Chulalongkorn Memorial Hospital(Bangkok,Thailand)were retrospectively *** status was evaluated via their vaccination *** for infections that occurred up to 5 years after liver transplantation were evaluated,and divided into VPIs and *** for cytomegalovirus and Epstein-Barr virus were *** of infection,length of hospital stay,ventilator support,intensive care unit requirement,and mortality were *** Seventy-seven children with a mean age of 3.29±4.17 years were included in the study,of whom 41(53.2%)were *** mean follow-up duration was 3.68±1.45 *** children(62.3%)had vaccination *** was a significant difference in the proportion of children with incomplete vaccination according to Thailand’s Expanded Program on Immunization(52.0%)and accelerated vaccine from Infectious Diseases Society of America(89.5%)(P<0.001).Post-liver transplant,47.9%of the children did not catch up with ageappropriate *** were 237 infections requiring hospitalization during the 5 years of *** were no significant differences in hospitalization for VPIs or non-VPIs in children with complete and incomplete *** risk of serious infection was high in the first year after receiving a liver transplant,and two children *** and gastroint

关键词： Children Hospitalization Immunization Liver transplant Thailand Vaccinepreventable infection

Liver tumors in children with chronic liver diseases

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World Journal of Gastrointestinal Oncology 2021年第11期13卷 1680-1695页

作者： Palittiya Sintusek Teerasak Phewplung Anapat Sanpavat yong poovorawan Thai Pediatric Gastroenterology Hepatology and Immunology Research UnitDepartment of PediatricsDivision of GastroenterologyFaculty of MedicineKing Chulalongkorn Memorial HospitalChulalongkorn UniversityBangkok 10330Thailand Department of Radiology Faculty of MedicineChulalongkorn UniversityKing Chulalongkorn Memorial HospitalBangkok 10330Thailand Department of Pathology Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand Center of Excellence in Clinical Virology Chulalongkorn UniversityBangkok 10330Thailand

Liver tumors are rare in children,but the incidence may increase in some circumstances and particularly in chronic liver *** liver tumors consequent to chronic liver diseases are malignant hepatocellular *** liver tumors include hepatoblastoma,focal nodular hyperplasia,adenoma,pseudotumor,and nodular regenerative *** of suspected cases is *** and surrogate markers of alpha-fetoprotein are used initially as noninvasive tools for ***,liver biopsy for histopathology evaluation might be necessary for patients with inconclusive *** the malignant liver tumor is detected in children with cirrhosis,liver transplantation is currently considered the preferred option and achieves favorable *** on the current evidence,this review focuses on liver tumors with underlying chronic liver disease,their epidemiology,pathogenesis,early recognition,and effective management.

关键词： Liver tumor Chronic liver disease Children Hepatocellular carcinoma Liver cancer Liver neoplasm

Eliminating viral hepatitis in children after liver transplants:How to reach the goal by 2030

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World Journal of Gastroenterology 2022年第3期28卷 290-309页

作者： Palittiya Sintusek Kessarin Thanapirom Piyawat Komolmit yong poovorawan The Thai Pediatric Gastroenterology Hepatology and Immunology(TPGHAI)Research UnitChulalongkorn UniversityBangkok 10330Thailand Division of Gastroenterology Department of PediatricsFaculty of MedicineKing Chulalongkorn Memorial HospitalChulalongkorn UniversityBangkok 10330Thailand Division of Gastroenterology Department of MedicineLiver Fibrosis and Cirrhosis Research UnitFaculty of MedicineChulalongkorn University and King Chulalongkorn Memorial HospitalThai Red Cross SocietyBangkok 10330Thailand Center of Excellence in Liver Diseases King Chulalongkorn Memorial HospitalChulalongkorn UniversityBangkok 10330Thailand Center of Excellence in Clinical Virology Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand

Viral hepatitis infections are a great burden in children who have received liver *** viruses can cause liver inflammation that can develop into liver graft fibrosis and cirrhosis over the long *** reactions due to viral hepatitis infections are associated with or can mimic graft rejection,rendering the condition difficult to *** strategies using vaccinations are agreeable to patients,safe,cost-effective and ***,strategies to eliminate viral hepatitis A and B focus mainly on immunization programmes for children who have received a liver *** a vaccine has been developed to prevent hepatitis C and E viruses,its use is not licensed ***,eliminating hepatitis C and E viruses mainly involves early detection in children with suspected cases and effective treatment with antiviral *** hygiene and sanitation are also important to prevent hepatitis A and E *** blood products and liver grafts should be screened for hepatitis B,C and E in children who are undergoing liver *** research on early detection of viral hepatitis infections should include molecular techniques for detecting hepatitis B and ***,novel antiviral drugs for eradicating viral hepatitis that are highly effective and safe are needed for children who have undergone liver transplantation.

关键词： Viral hepatitis Children Adolescent Liver transplantation Infection Elimination

Increased osteopontin and liver stiffness measurement by transient elastography in biliary atresia

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World Journal of Gastroenterology 2010年第43期16卷 5467-5473页

作者： Sittisak Honsawek Maneerat Chayanupatkul Voranush Chongsrisawat Paisarn Vejchapipat yong poovorawan Department of Biochemistry Faculty of Medicine Chulalongkorn University and King Chulalongkorn Memorial Hospital Department of Physiology Faculty of Medicine Chulalongkorn University Center of Excellence in Clinical Virology Department of Pediatrics Faculty of Medicine Chulalongkorn University Department of Surgery Faculty of Medicine Chulalongkorn University

AIM: To analyze plasma osteopontin levels and liver stiffness using transient elastography in postoperative biliary atresia (BA) children compared with healthy controls. METHODS: Thirty children with postoperative BA and 10 normal controls were enrolled. The patients were categorized into two groups according to their jaundicestatus. Plasma levels of osteopontin were determined using commercially available enzyme-linked immunosorbent assay. Liver stiffness was measured by using transient elastography (Fibroscan). Ten validated Fibroscan measurements were performed in each patient and control with the result expressed in kilopascals (kPa). RESULTS: Plasma osteopontin was significantly elevated in BA children compared with that of healthy controls (47.0 ± 56.4 ng/mL vs 15.1 ± 15.0 ng/mL, P = 0.01). The liver stiffness measurement was markedly elevated in the patients with BA compared with that of controls (26.9 ± 24.6 kPa vs 3.9 ± 0.7 kPa, P = 0.001). Subgroup analysis showed that the BA patients with jaundice had more pronounced plasma osteopontin levels than those without jaundice (87.1 ± 61.6 ng/mL vs 11.9 ± 6.1 ng/mL, P = 0.001). Furthermore, the mean liver stiffness was significantly greater in the jaundiced BA patients compared with non-jaundiced patients (47.7 ± 21.8 kPa vs 8.7 ± 3.0 kPa, P = 0.001). Additionally, plasma osteopontin was positively related to serum total bilirubin (r = 0.64, P < 0.001). There was also a correlation between plasma osteopontin and liver stiffness values (r = 0.60, P < 0.001). CONCLUSION: High plasma osteopontin positively correlated with degree of hepatic fibrosis and could be used as a biochemical parameter reflecting disease severity in postoperative BA children.

关键词： Biliary atresia Fibroscan Jaundice Liver stiffness Osteopontin

Cytokine levels in patients with chikungunya virus infection

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Asian Pacific Journal of Tropical Medicine 2013年第8期6卷 631-634页

作者： Chintana Chirathaworn yong poovorawan Somrat Lertmaharit Norra Wuttirattanakowit Department of Microbiology Faculty of Medicine Chulalongkorn University Department of Pediatrics Faculty of Medicine Chulalongkorn University Department of Preventive and Social Medicine Faculty of Medicine Chulalongkorn University Narathiwatratchanakharin Hospital

Objective: To investigate cytokine profile in patients with chikungunya virus (CHIKV) infection. Methods: Twenty eight pairs of serum samples collected from CHIKV infected patients during the outbreak of chikungunya fever in South Thailand in 2008 were obtained. A multiple cytokine assay for detection of 17 cytokines was performed. Results: In the acute stage of CHIKV infection, the patients had significantly higher levels of interleukin-6, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, monocyte chemotactic protein 1 and tumor necrosis factor alpha than the control ( P<0.001, P=0.023, P=0.015, P <0.001 and P=0.024, respectively). When the disease developed to the recovery stage, the patients had significantly lower levels of interleukin-6, granulocyte-macrophage colony-stimulating factor, monocyte chemotactic protein 1 and macrophage inflammatory protein beta than in the acute stage ( P<0.001). Conclusions: This study provides additional information that these cytokines could play roles in pathogenesis of CHIKV infection and could be used as disease biomarkers or drug targets.

关键词： Chikungunya virus Cytokine Interleukin-6 Granulocyte-macrophage colonystimulating factor Monocyte chemotactic protein 1

Early viral kinetics during hepatitis C virus genotype 6 treatment according to IL28B polymorphisms

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World Journal of Gastroenterology 2014年第30期20卷 10599-10605页

作者： Srunthron Akkarathamrongsin Sunchai Payungporn Vo Duy Thong Kittiyod poovorawan Phisit Prapunwattana yong poovorawan Pisit Tangkijvanich Center of Excellence in Clinical Virology Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand Department of Biochemistry Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand Department of Medicine Faculty of MedicineChulalongkorn UniversityBangkok 10330Thailand

AIM: To investigate the early viral kinetics and interleukin-28B (IL28B) polymorphisms of hepatitis C genotype 6 during pegylated interferon and ribavirin therapy.

关键词： Hepatitis C virus Genotype 6 Interleukin28B rs12979860 Early viral kinetics