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Bovine Serum Albumin-Conjugated Ferrimagnetic Iron Oxide Nanoparticles to Enhance the Biocompatibility and Magnetic Hyperthermia Performance

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Nano-Micro Letters 2016年第1期8卷 80-93页

作者： Viveka Kalidasan Xiao Li Liu Tun Seng Herng yong Yang jun Ding Department of Materials Science and Engineering Faculty of Engineering National University of Singapore Shaanxi Key Laboratory of Degradable Biomedical Materials School of Chemical Engineering Northwest University

Magnetic hyperthermia is a fast emerging, non-invasive cancer treatment method which is used synergistically with the existing cancer therapeutics. We have attempted to address the current challenges in clinical magnetic hyperthermia-improved biocompatibility and enhanced heating characteristics, through a single combinatorial approach. Both superparamagnetic iron oxide nanoparticles(SPIONs) of size 10 nm and ferrimagnetic iron oxide nanoparticles(FIONs) of size 30 nm were synthesized by thermal decomposition method for comparison studies. Two different surface modifying agents, viz, Cetyl Trimethyl Ammonium Bromide and 3-Aminopropyltrimethoxysilane, were used to conjugate Bovine Serum Albumin(BSA) over the iron oxide nanoparticles via two different methods—surface charge adsorption and covalent amide bonding, respectively. The preliminary haemolysis and cell viability experiments show that BSA conjugation mitigates the haemolytic effect of the iron oxide nanoparticles on erythrocytes and is non-cytotoxic to the healthy Baby Hamster Kidney cells. It was observed from the results that due to better colloidal stability, the SAR value of the BSA-iron oxide nanoparticles is higher than the iron oxide nanoparticles without BSA, irrespective of the size of the iron oxide nanoparticles and method of conjugation. The BSA-FIONs seem to show improved biocompatibility, as the haemolytic index is less than 2 % and cell viability is up to 120 %, when normalized with the control. The SAR value of BSAFIONs is 2300 Wg^(-1) when compared to 1700 Wg^(-1) of FIONs without BSA conjugation. Thus, we report here that BSA conjugation over FIONs(with a high saturation magnetization of 87 emug^(-1)) provide a single combinatorial approach to improve the biocompatibility and enhance the SAR value for magnetic hyperthermia, thus addressing both the current challenges of the same.

关键词： Magnetic hyperthermia Ferrimagnetic iron oxide nanoparticles Bovine serum albumin Haemolysis Cell viability Specific absorption rate

Analysis of Translocation of the CagA Protein and Induction of a Scattering Phenotype in AGS Cells Infected with Helicobacter pylori

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Biomedical and Environmental Sciences 2009年第5期22卷 394-400页

作者： XIAN-HONG LIANG YA-NAN ZHANG yong-jun WANG XI-XIONG KANG Department of Neurology Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Microbiology Peking University Health Science Center Beijing 100191 China Department of Laboratory Beijing Tiantan Hospital Capital Medical UniversityBeijing 100050 China

Objective To investigate whether the presence of structured CagA proteins in Western- and Eastern-type Helicobacter pylori （H. pylori） induces different incidences of gastric diseases. Methods CagA and phosphorylatd CagA were expressed in AGS gastric epithelial cells infected with wild type and mutant strains. The ability of individual CagA was determined by immunoprecipitation and Western blot assay. Morphological changes of these cells were observed under microscope to evaluate the appearance of elongation hummingbird phenotype. Results The sizes of CagA proteins in different strains were different, and no phosphorylated CagA proteins were detected in wild-type strains. Meanwhile, the kinetics of CagA status in AGS infected with H. pylori was detected. The molecular weight of phosphorylated CagA with the same size of CagA proteins in H. pylori was different in infections with different wild-type strains. CagA and phosphorylated CagA increased in a time-dependent manner after the infection. The hummingbird phenotype with H. pylori for time-course was observed under microscope. Instead of HPK5 strain, the wild-type 26695 strain induced hummingbird phenotype in a time-dependent manner. Conclusion Translocation and phosphorylation of CagA are necessary, but not sufficient, for the induction of hummingbird phenotype in AGS cells.

关键词： CagA Western CagA-specific sequence (WSS) Eastern CagA-specific sequence (ESS) Hummingbird phenotype

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某骨科医院加强运营管理的实践与思考

现代医院管理 2024年第2期22卷 40-42页

作者：岳秋颖芮永军郁晓静林敏无锡市第九人民医院江苏省无锡市214000

加强运营管理是公立医院高质量发展的必然要求。某三甲骨科医院针对医院面临的主要问题,开展了一系列加强医院精细化运营管理的实践,以加强专科建设、提高服务能力和效率、提升患者满意度为重点,优化绩效考核,加强运营成本控制,构建具... 详细信息

加强运营管理是公立医院高质量发展的必然要求。某三甲骨科医院针对医院面临的主要问题,开展了一系列加强医院精细化运营管理的实践,以加强专科建设、提高服务能力和效率、提升患者满意度为重点,优化绩效考核,加强运营成本控制,构建具有专科特色的运营管理模式。有效提升了医疗服务质量和科室运营管理效率,全面推动医院高质量发展。

关键词：公立医院高质量发展精细化管理运营管理

宽温区Yb∶YAG板条激光模块热畸变仿真研究

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激光与红外 2024年第4期54卷 503-507页

作者：朱铎宋艳洁陈中正许元斋薄勇彭钦军中国科学院物理与化学技术研究所固体激光重点实验室北京100190 中国科学院理化技术研究所功能晶体与激光技术重点实验室北京100190 中国科学院大学北京100190 齐鲁中科光学物理与工程技术研究所山东济南250000

本文对不同冷却温度下的Yb:YAG晶体板条激光模块高功率运转时的温度场、应力场及对应的热畸变进行了仿真分析研究。结果显示,随着冷却温度从300 K降低至77K,板条的温度梯度热应力与应变都明显降低。当冷却温度为77 K时,最大主应力为4.14... 详细信息

本文对不同冷却温度下的Yb:YAG晶体板条激光模块高功率运转时的温度场、应力场及对应的热畸变进行了仿真分析研究。结果显示,随着冷却温度从300 K降低至77K,板条的温度梯度热应力与应变都明显降低。当冷却温度为77 K时,最大主应力为4.14 MPa,仅为常温时的15.6%,最大主应变为3.82×10^(-5),仅为常温时的6%。为了分析Yb:YAG晶体板条激光模块不同冷却温度下输出激光的光束质量,以确定其最佳运转温度,我们采用光线追迹的方法,对单程通过板条的1030 nm探测光进行仿真。可以看到,当冷却温度为77 K时,远场光斑能量更为集中,且探测光光程差的PV值为0.7941μm,仅为300 K时的59.6%。模拟结果表明低温运转有利于Yb:YAG晶体板条激光模块产生高功率高光束质量激光输出,这为高功率高光束质量Yb:YAG晶体板条激光的设计工作奠定了基础。

关键词：板条激光器 Yb∶YAG 热效应仿真模拟

Catalytical oxidation of styrene by molecularly imprinted polymer with phenylacetic acid as template and hemin as co-monomer

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Chinese Chemical Letters 2007年第8期18卷 981-984页

作者： jun Wang Zhi yong Chen Mei Ping Zhao Yuan Zong Li The Key Laboratory of Bioorganic Chemistry and Molecular Engineering College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

This letter used the molecular imprinting technology to build up the microenvironment around co-monomer bemin to mimic the cytochrome P450 catalyzing the epoxidation of styrene. The results showed that the conversion rates of products were obviously enhanced by molecularly imprinted polymers, compared to free heroin solution, using three kinds of oxidants. The used axial ligand in polymers synthesis also improved the total conversion rates.

关键词： Molecularly imprinted polymers Enzyme mimic Cytochrome P450 Styrene Epoxidation

QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

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Journal of Integrative Agriculture 2017年第2期16卷 435-444页

作者： ZHOU Sheng-hui FU Lin wu Qiu-hong CHEN Jiao-jiao CHEN yong-xing XIE Jing-zhong WANG Zhen-zhong WANG Guo-xin ZHANG De-yun LIANG yong ZHANG Yan YOU Ming-shan LIANG Rong-qi HAN jun LIU Zhi-yong State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 P.R. China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 P.R. China China Rural Technology Development Center Beijing 100045 P.R.China College of Plant Science and Technology Beijing University of Agriculture Beijing 102206 P.R.China

Pre-harvest sprouting （PHS） occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci （QTL） for PHS resistance were mapped using an available high-density single nucleotide polymorphism （SNP） and simple sequence repeat （SSR） genetic linkage map developed from a 269 recombinant inbred lines （RILs） population of Yanda 1817xBeinong 6. Using phenotypic data on two locations （Beijing and Shijiazhuang, China） in two years （2012 and 2013 harvesting seasons）, five QTLs, designated as ***-3A. 1, ***-3A.2, ***-5B, ***-4A, and ***-6A, for PHS （GP） were detected by inclusive composite interval mapping （ICIM）（LOD≥2.5）. Two major QTLs, ***-3A.2 and ***-5B, were mapped on 3AL and 5BS chromosome arms, explaining 6.29-21.65% and 4.36-5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the TaVp-1A flanking region on 3AL is responsible for ***-3A.2. SNP markers flanking ***-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection （MAS）.

关键词： wheat pre-harvest sprouting quantitative trait loci SNP TaVp-IA

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引入神经网络极限学习机的关键数据查询模型

计算机仿真 2024年第3期41卷 519-523页

作者：张勇飞陈艳君赵世忠南昌大学科学技术学院江西九江332020 南昌大学工程建设学院江西南昌330031

网络空间数据的结构具有较高相似性,海量数据的不断增量更新,导致关键数据查询结果存在冗余和偏离问题。因此提出基于神经网络极限学习机的关键数据查询方法。建模描述关键数据查询问题。基于此引入神经网络极限学习机,建立关键数据查... 详细信息

网络空间数据的结构具有较高相似性,海量数据的不断增量更新,导致关键数据查询结果存在冗余和偏离问题。因此提出基于神经网络极限学习机的关键数据查询方法。建模描述关键数据查询问题。基于此引入神经网络极限学习机,建立关键数据查询模型。预处理数据库中无用数据和重复数据做,通过输出权值范数的最小二乘解,避免算法陷入局部最优。结合输出矩阵,训练查询模型,输出结果结果即为关键数据查询结果。为证明上述方法的性能优势,设计对比实验,结果表明提出的方法应用于关键数据查询的均方根误差不超过1.2,平均绝对百分比误差最高为4.1%,关系数F可达0.6,网络节点的使用率低于20%。以上实验数据验证了上述方法数据查询精度较高,可应用性更强。

关键词：神经网络极限学习机关键数据输出权值最小二乘解数据预处理

Effects of Chemical Components on the Amount of Green Tea Cream

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Agricultural Sciences in China 2011年第6期10卷 969-974页

作者： XU yong-quan CHEN Su-qin SHEN Dan-yu YIN jun-feng Key Laboratory of Machining and Quality Control of Tea and Beverage Plants Ministry of Agriculture/National Engineering Technology Research Center for Tea Industry/Tea Research Institute Chinese Academy of Agrieultural Sciences Hangzhou 310008 P.R.China Shenzhen ShenbaoHuacheng Foods Co. Ltd Shenzhen 518020 P.P.China Research Institute of Subtropical Forestry Chinese Academy of Forestry Fuyang 311400 P.R. China

The relationship between the amount of tea cream and the chemical components contents in the original green tea infusion was investigated. The results showed that the amounts of tea cream in the green tea infusions obtained from different cultivars and different parts of new shoots were varied. There were many chemical components participating in the formation of green tea cream. However, there were only the contents of caffeine （Y=0.85, P〈0.01） and polyphenols （Y=0.65, P〈0.05） in the original green tea infusion highly correlated with the amount of green tea cream. Stepwise regression analysis of overall chemical components indicated that the contents of caffeine and gallated catechins in the original green tea infusion had a significant effect （P〈0.01） on green tea cream levels. Cream （g L-1）=-172.071＋ 0.129×Ccaffeine＋0.024×Ggallated catechins （R2=0.936）. The amount of green tea cream can be predicted by the contents of gallated catechins and caffeine in the original tea infusion. Principal component analysis also indicated that catechins, minerals, and polysaccharides were the important chemical components in the formation of green tea cream.

关键词： green tea infusion chemical components amount of tea cream

FSI simulation of dynamics of fish passing through a tubular turbine based on the immersed boundary-lattice Boltzmann coupling scheme

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Journal of Hydrodynamics 2022年第1期34卷 135-147页

作者： Ze-hao Huang yong-guang Cheng Jia-yang wu Wei Diao Wen-xin Huai State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan UniversityWuhan430072China Changjiang Institute of Survey PlanningDesign and ResearchWuhan430010China School of River and Ocean Engineering Chongqing Jiaotong UniversityChongqing400016China

The anadromous fish can pass through turbines of run-of-the-river hydropower stations to reach the downstream watershed, but their mortality is significant because of the complex turbine structure, the fast-rotating runner, and the special flow patterns. Numerical simulations of the dynamics of fish passing are a challenging task, because the fish motion in the turbines involves a strong fluid-structure interaction (FSI). In this paper, the 3-D immersed boundary-lattice Boltzmann (IB-LB) coupling scheme is proposed to treat the FSI between the water and the fish. The process of one fish and three fish passing through a tubular turbine is simulated on a graphics processing unit (GPU) platform. The fish motion postures (translation and rotation), the fish body pressure distributions and histories are analyzed, and the results are consistent with the previous studies. This paper presents the IB-LB models, the simulation procedures, the specific treatments, and related results, to demonstrate the effectiveness of the IB-LB coupling scheme in simulating FSI problems and its application prospects in developing fish-friendly turbines.

关键词： Fluid-structure interaction(FSI) immersed boundary method lattice Boltzmann method tubular turbine fish passing dynamics 3-D simulation