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Upregulation of miR-34c after silencing E2F transcription factor 1 inhibits paclitaxel combined with cisplatin resistance in gastric cancer cells

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World Journal of Gastroenterology 2020年第5期26卷 499-513页

作者： Hong Zheng Jin-Jing Wang Xiao-Rong Yang yong-lin yu Department of Pathology the Affiliated Hospital of Zunyi Medical University

BACKGROUND MicroRNA 34c(miR-34c)has been reported to be associated with malignant types of cancer,however,it remains unknown whether miR-34c is involved in chemoresistance in gastric cancer(GC).AIM To investigate the effect of miR-34c and its upstream transcription factor E2F1 on paclitaxel combined with cisplatin resistance in GC *** Paired GC tissues and adjacent normal tissues were randomly sampled from 74 GC ***-34c and E2F1 were detected by real-time quantitative PCR(qPCR)and Western *** addition,the drug resistance of GC cells to paclitaxel and cisplatin was induced by concentration gradient increasing methods,and changes in miR-34c and E2F1 during this process were ***,E2F1 and miR-34c overexpression or underexpression vectors were constructed and transfected into drug-resistant GC *** was employed to test the sensitivity of cells to paclitaxel combined with cisplatin,qPCR was adopted to detect the expression of miR-34c,Western blot was applied to detect the expression levels of E2F1,drug resistance-related proteins and apoptosis-related proteins,and flow cytometry was used for the determination of cell apoptosis and cell cycle *** E2F1 was overexpressed while miR-34c was underexpressed in *** inducing GC cells to be resistant to paclitaxel and cisplatin,E2F1 expression increased while miR-34c expression *** silencing E2F1 and overexpressing miR-34c could increase the sensitivity of drug-resistant GC cells to paclitaxel combined with cisplatin,promote cell apoptosis and inhibit cell *** which,silencing E2F1 could reduce the expression of drug resistance-related proteins and apoptosis-related proteins,while over-expression of miR-34c could upregulate the expression of apoptosis-related proteins without affecting the expression of MDR-1,MRP and other drug resistance-related *** experiments demonstrated that inhibiting miR-34c could significantly weaken the sensi

关键词： E2F transcription factor 1 MicroRNA 34c Gastric cancer Paclitaxel combined with cisplatin resistance

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Elimination of methicillin‑resistant Staphylococcus aureus biofilms on titanium implants via photothermally‑triggered nitric oxide and immunotherapy for enhanced osseointegration

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Military Medical Research 2024年第2期11卷 157-179页

作者： yong‑lin yu Jun‑Jie Wu Chuan‑Chuan lin Xian Qin Franklin R.Tay Li Miao Bai‑Long Tao Yang Jiao Department of Pathology Affiliated Hospital of Zunyi Medical UniversityZunyi 563003GuizhouChina Laboratory Research Center the First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Blood Transfusion Laboratory of Radiation Biologythe Second Affiliated HospitalArmy Military Medical UniversityChongqing 400037China Department of Reproductive Endocrinology Chongqing Health Center for Women and ChildrenChongqing 401147China The Graduate School Augusta UniversityAugustaGA 30912USA Department of Stomatology the Seventh Medical Center of PLA General HospitalBeijing 100700China

Background:Treatment of methicillin-resistant Staphylococcus aureus(MRSA)biofilm infections in implant placement surgery is limited by the lack of antimicrobial activity of titanium(Ti)*** is a need to explore more effective approaches for the treatment of MRSA biofilm ***:Herein,an interfacial functionalization strategy is proposed by the integration of mesoporous polydopamine nanoparticles(PDA),nitric oxide(NO)release donor sodium nitroprusside(SNP)and osteogenic growth peptide(OGP)onto Ti implants,denoted as Ti-PDA@*** physical and chemical properties of Ti-PDA@SNP-OGP were assessed by scanning electron microscopy,X-ray photoelectron spectroscope,water contact angle,photothermal property and NO release *** synergistic antibacterial effect and elimination of the MRSA biofilms were evaluated by 2′,7′-dichlorofluorescein diacetate probe,1-N-phenylnaphthylamine assay,adenosine triphosphate intensity,O-nitrophenyl-β-D-galactopyranoside hydrolysis activity,bicinchoninic acid *** staining,assays for alkaline phosphatase activity,collagen secretion and extracellular matrix mineralization,quantitative real‑time reverse transcription‑polymerase chain reaction,and enzyme-linked immunosorbent assay(ELISA)were used to evaluate the inflammatory response and osteogenic ability in bone marrow stromal cells(MSCs),RAW264.7 cells and their co-culture *** staining,ELISA,micro-CT,hematoxylin and eosin,Masson's trichrome and immunohistochemistry staining were used to evaluate the eradication of MRSA biofilms,inhibition of inflammatory response,and promotion of osseointegration of Ti-PDA@SNP-OGP in ***:Ti-PDA@SNP-OGP displayed a synergistic photothermal and NO-dependent antibacterial effect against MRSA following near-infrared light(NIR)irradiation,and effectively eliminated the formed MRSA biofilms by inducing reactive oxygen species(ROS)-mediated oxidative stress,destroying bacterial membrane integrity and

关键词： Polydopamine nanoparticles Methicillin-resistant Staphylococcus aureus Nitric oxide Osseointegration Osteo-immunomodulation Photothermal effect Titanium implants

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Exosomal mi R-182 regulates the effect of RECK on gallbladder cancer

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World Journal of Gastroenterology 2020年第9期26卷 933-946页

作者： Hong Zheng Jin-Jing Wang Li-Jin Zhao Xiao-Rong Yang yong-lin yu Department of Pathology Affiliated Hospital of Zunyi Medical UniversityZunyi 563003Guizhou ProvinceChina Department of Hepatopancreatobiliary Surgery Affiliated Hospital of Zunyi Medical UniversityZunyi 563003Guizhou ProvinceChina

BACKGROUND As the most common biliary malignancy,gallbladder cancer(GC)is an elderlybiased *** extensive studies have elucidated the molecular mechanism of microRNA 182(miR-182)and reversion-inducing-cysteine-rich protein with kazal motifs(RECK)in various cancers,the specific role of exosomal miR-182 and RECK in GC remains poorly *** To explore the relationship between exosomal miR-182/RECK and metastasis of *** Paired GC and adjacent normal tissues were collected from 78 *** polymerase chain reaction was employed to detect miR-182 and exosomal miR-182 expression,and Western blotting was conducted to determine RECK *** addition,the effects of exosomal miR-182/RECK on the biological function of human GC cells were ***,the double luciferase reporter gene assay was applied to validate the targeting relationship between miR-182 and *** Compared with normal gallbladder epithelial cells,miR-182 was highly expressed in GC cells,while RECK had low *** miR-182 could be absorbed and transferred by *** miR-182 inhibited RECK expression and promoted the migration and invasion of GC *** Exosomal miR-182 can significantly promote the migration and invasion of GC cells by inhibiting RECK;thus miR-182 can be used as a therapeutic target for GC.

关键词： Exosome miR-182 RECK Gallbladder cancer Quantitative polymerase chain reaction Therapeutic target

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Novel mutations of the ATP7B gene in Han Chinese families with pre-symptomatic Wilson's disease

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World Journal of Pediatrics 2015年第3期11卷 255-260页

作者： Zhe-Feng yuan Wei Wu yong-lin yu Jue Shen Shan-Shan Mao Feng Gao Zhe-Zhi Xia Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310003China Department of Endocrinology Children's HospitalZhejiang University School of MedicineHangzhou 310003China

Background:Wilson's disease(WD)is an autosomal recessive genetic disorder of copper metabolism,caused by mutations in the ATP7B gene,resulting in copper accumulation in the liver,brain,kidney,and cornea and leading to significant disability or death if *** diagnosis and proper therapy usually predict a good prognosis,especially in pre-symptomatic *** testing is the most accurate and effective diagnostic method for early ***:The clinical and biochemical features of three unrelated Han Chinese families with pre-symptomatic WD were *** molecular defects in these families were investigated by polymerase chain reaction and DNA *** healthy children with the same ethnic background served as *** tools(polymorphism phenotyping-2,sorting intolerant from tolerant,protein analysis through evolutionary relationships,and predictor of human deleterious single nucleotide polymorphisms)were combined and used to predict the functional effects of ***:We identified 2 novel ATP7B mutations(***692Pro and ***728Ser)and 3 known mutations(***769fs,***778Leu and ***216Met)in these Chinese WD *** mutations were not observed in the 100 normal *** bioinformatic method showed that ***692Pro and ***728Ser mutations are ***:Our research enriches the mutation spectrum of the ATP7B gene worldwide and provides valuable information for studying the mutation types and mode of inheritance of ATP7B in the Chinese *** function analysis and genetic testing in young children with WD are necessary to shorten the time to the initiation of therapy,reduce damage to the liver and brain,and improve prognosis.

关键词： ATP7B Chinese mutation Wilson's disease

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Study on Insertion Loss of Fiber Fabry-Perot Filters

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Journal of Computer and Communications 2013年第7期1卷 62-66页

作者： Sheng-Nan Su Hai-Bing Qi yong-lin yu Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan China.

Fiber Fabry-Perot (FFP) filters have been widely used in optical fiber communications, and insertion loss (IL) is one of its important characteristics. Based on theoretically analysis, factors related to IL were discussed. In order to investigate the IL of different structures, simulations are carried out with finite-difference time-domain (FDTD) algorithm. Comparisons are made between the optimized structure and full-size-mirror FFP filter, and fiber-inserted FFP filter as well. Simulation results demonstrated that the finite- size-mirror structure can confine the mode size of the open resonator, hence reduce the IL of FFP filter.

关键词： FFP Filter Insertion Loss Finesse

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Nanostructured ZnO/BiVO_(4)I-scheme heterojunctions for piezocatalytic degradation of organic dyes via harvesting ultrasonic vibration energy

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 488-497页

作者： Yiling Li Xiaoyao yu Yingjie Zhou Yao lin Ying Wu Key Laboratory of the Ministry of Education for Advanced Catalysis Materials College of Chemistry and Materials ScienceZhejiang Normal UniversityJinhua 321004China

BiVO_(4)porous spheres modified by ZnO were designed and synthesized using a facile two-step *** resulting ZnO/BiVO_(4)composite catalysts have shown remarkable efficiency as piezoelectric catalysts for degrading Rhodamine B(RhB)unde mechanical vibrations,they exhibit superior activity compared to pure *** 40wt%ZnO/BiVO_(4)heterojunction composite displayed the highest activity,along with good stability and *** enhanced piezoelectric catalytic activity can be attributed to the form ation of an I-scheme heterojunction structure,which can effectively inhibit the electron-hole ***,hole(h+)and superoxide radical(·O_(2)^(-))are proved to be the primary active ***,ZnO/BiVO_(4)stands as an efficient and stable piezoelectric catalyst with broad potential application in the field of environmental water pollution treatment.

关键词： piezoelectric catalytic heterojunction dye degradation ultrasonic vibration

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Numerical Study for Turbulent Flow of Drag and Flow Noise with Wavy Wall

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Journal of Shanghai Jiaotong university(Science) 2011年第1期16卷 45-54页

作者：孟堃宇张怀新史勇林潘雨村 School of Naval Architecture Ocean and Civil EngineeringShanghai Jiaotong University

In this paper,a numerical simulation of flow-induced noise by the low Mach number turbulent flow with a sinusoidal wavy wall was presented based on the unsteady incompressible Navier-Stokes equations and Lighthill's acoustic *** eddy simulation (LES) was used to investigate the space-time flow field and the Smagorinsky sub-grid scale (SGS) model was introduced for turbulence *** Lighthill's acoustics analogy,the flow field simulated by LES was taken as near-field sound sources and radiated sound from turbulent flow was computed by the Curle's integral formulation under the low Mach number *** spanwise wavy wall and streamwise wavy wall with various wall wave amplitudes were discussed to investigate their effects on reducing the drag and flow *** relationship between flow noise and drag on the wavy wall is also studied.

关键词： large eddy simulation (LES) wavy wall riblets turbulent flow flow noise

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Boosting bidirectional sulfur conversion enabled by introducing boron-doped atoms and phosphorus vacancies in Ni2P for lithium-sulfur batteries

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Journal of Energy Chemistry 2025年第1期100卷 760-769页

作者： lin Peng yu Bai Hang Li Meixiu Qu Zhenhua Wang Kening Sun Advanced Research Institute of Multidisciplinary Science Beijing Institute of Technology Beijing Key Laboratory of Chemical Power Source and Green Catalysis School of Chemistry and Chemical Engineering Beijing Institute of Technology

Lithium-sulfur （Li-S） batteries have gained great attention due to the high theoretical energy density and low cost,yet their further commercialization has been obstructed by the notorious shuttle effect and sluggish redox ***,we supply a strategy to optimize the electron structure of Ni2P by concurrently introducing B-doped atoms and P vacancies in Ni2P （Vp-B-Ni2P）,thereby enhancing the bidirectional sulfur *** study indicates that the simultaneous introduction of B-doped atoms and P vacancies in Ni2P causes the redistribution of electron around Ni atoms,bringing about the upward shift of d-band center of Ni atoms and effective d-p orbital hybridization between Ni atoms and sulfur species,thus strengthening the chemical anchoring for lithium polysulfides （LiPSs） as well as expediting the bidirectional conversion kinetics of sulfur ***,theoretical calculations reveal that the incorporation of B-doped atoms and P vacancies in Ni2P selectively promotes Li2S dissolution and nucleation ***,the Li-S batteries with Vp-B-Ni2P-separators present outstanding rate ability of 777 m A h g-1at 5 C and high areal capacity of 8.03 mA h cm-2under E/S of 5μL mg-1and sulfur loading of 7.20 mg *** work elucidates that introducing heteroatom and vacancy in metal phosphide collaboratively regulates the electron structure to accelerate bidirectional sulfur conversion.

关键词： Lithium sulfur batteries

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面向推荐多样性改进的概率传播模型

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北京邮电大学学报 2016年第3期39卷 34-38页

作者：蔡国永张东林煜明文益民桂林电子科技大学广西可信软件重点实验室桂林541004

针对基于二部图的概率传播(ProbS)模型以优化推荐列表的精确度为目标,而忽略了推荐多样性的问题,提出了改进的概率传播(iProbS)模型.iProbS将项目得分预测过程分解为资源的3步传播过程,每步传播包含传播概率和传播损耗.设计传播概率时,... 详细信息

针对基于二部图的概率传播(ProbS)模型以优化推荐列表的精确度为目标,而忽略了推荐多样性的问题,提出了改进的概率传播(iProbS)模型.iProbS将项目得分预测过程分解为资源的3步传播过程,每步传播包含传播概率和传播损耗.设计传播概率时,考虑的因素是用户评分;设计传播损耗时,则分别考虑了项目的度、用户熵和邻居项目.通过在2个常用数据集MovieLens和Netflix上的大量不同实验,证明了iProbS算法在推荐准确率、推荐整体多样性、推荐个体多样性以及销售平衡4个方面均比ProbS模型性能更好.最后按不同的推荐步骤分析了iProbS算法的计算复杂度.

关键词：推荐系统多样性概率传播二部图

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PD-YOLO:Colon Polyp Detection Model Based on Enhanced Small-Target Feature Extraction

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Computers, Materials & Continua 2025年第1期82卷 913-928页

作者： Yicong yu Kaixin lin Jiajun Hong Rong-Guei Tsai yuanzhi Huang New Engineering Industry College Putian UniversityPutian351100China Engineering Research Center of Big Data Application in Private Health Medicine(Putian University) Fujian Province UniversityPutian351100China Computer College Guangdong University of Science&TechnologyDongguan523668China

In recent years,the number of patientswith colon disease has increased *** polyps are the precursor lesions of colon *** not diagnosed in time,they can easily develop into colon cancer,posing a serious threat to patients’lives and health.A colonoscopy is an important means of detecting colon ***,in polyp imaging,due to the large differences and diverse types of polyps in size,shape,color,etc.,traditional detection methods face the problem of high false positive rates,which creates problems for doctors during the diagnosis *** order to improve the accuracy and efficiency of colon polyp detection,this question proposes a network model suitable for colon polyp detection(PD-YOLO).This method introduces the self-attention mechanism CBAM(Convolutional Block Attention Module)in the backbone layer based on YOLOv7,allowing themodel to adaptively focus on key information and ignore the unimportant *** help themodel do a better job of polyp localization and bounding box regression,add the SPD-Conv(Symmetric Positive Definite Convolution)module to the neck layer and use deconvolution instead of *** results indicate that the PD-YOLO algorithm demonstrates strong robustness in colon polyp *** to the original YOLOv7,on the Kvasir-SEG dataset,PD-YOLO has shown an increase of 5.44 percentage points in AP@0.5,showcasing significant advantages over other mainstream methods.

关键词： Polyp detection YOLOv7 SPD-Conv CBAM deconvolution

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