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Endoscopic ultrasonography guided-fine needle aspirationfor the diagnosis of solid pancreaticobiliary lesions:Clinicalaspects to improve the diagnosis

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World Journal of Gastroenterology 2016年第2期22卷 628-640页

作者： Hiroyuki Matsubayashi Toru Matsui yohei yabuuchi Kenichiro Imai Masaki Tanaka Naomi Kakushima Keiko Sasaki Hiroyuki Ono Division of Endoscopy Shizuoka Cancer CenterSuntogunShizuoka 411-8777Japan Division of Pathology Shizuoka Cancer CenterSuntogunShizuoka 411-8777Japan

Endoscopic ultrasonography-guided fine-needle aspiration(EUS-FNA) has been applied to pancreaticobiliary lesions since the 1990 s and is in widespread use throughout the world today. We used this method to confirm the pathological evidence of the pancreaticobiliary lesions and to perform suitable therapies. Complications of EUS-FNA are quite rare, but some of them are severe. Operators should master conventional EUS observation and experience a minimum of 20-30 cases of supervised EUS-FNA on non-pancreatic and pancreatic lesions before attempting solo EUSFNA. Studies conducted on pancreaticobiliary EUSFNA have focused on selection of suitable instruments(e.g., needle selection) and sampling techniques(e.g., fanning method, suction level, with or without a stylet, optimum number of passes). Today, the diagnostic ability of EUS-FNA is still improving; the detection of pancreatic cancer(PC) currently has a sensitivity of 90%-95% and specificity of 95%-100%. In addition to PC, a variety of rare pancreatic tumors can be discriminated by conducting immunohistochemistry on the FNA materials. A flexible, large caliber needle has been used to obtain a large piece of tissue, which can provide sufficient histological information to be helpful in classifying benign pancreatic lesions. EUSFNA can supply high diagnostic yields even for biliary lesions or peri-pancreaticobiliary lymph nodes. This review focuses on the clinical aspects of EUS-FNA in the pancreaticobiliary field, with the aim of providing information that can enable more accurate and efficient diagnosis.

关键词： Endoscopic ultrasonography-guided fineneedleaspiration Diagnosis Pancreaticobiliary Pancreatic Cancer

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Colovesical fistula caused by glucocorticoid therapy for IgG4-related intrapelvic mass

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World Journal of Clinical Cases 2015年第12期3卷 1000-1004页

作者： yohei yabuuchi Hiroyuki Matsubayashi Masato Matsuzaki Akio Shiomi Michihisa Moriguchi Ichiro Kawamura Ichiro Ito Hiroyuki Ono Division of Endoscopy Shizuoka Cancer Center Division of Urology Shizuoka Cancer Center Division of Colon Surgery Shizuoka Cancer Center Division of Intervention Radiology Shizuoka Cancer Center Division of Infectious Diseases Shizuoka Cancer Center Division of Pathology Shizuoka Cancer Center

IgG4-related disease(IgG4-RD) is an immune-mediated fibroinflammatory disorder that can occur in almost all systemic organs and generally responds to corticosteroid treatment. We report a rare case of an IgG4-related intrapelvic mass lesion that responded to steroid therapy but caused a fistula between the sigmoid colon and bladder. A 71-year-old man was followed after treatment for hepatocellular carcinoma. Follow-up computed tomography(CT) incidentally depicted left hydronephrosis with an ill-demarcated intrapelvic mass lesion. This lesion was histologically diagnosed as IgG4-RD by open biopsy, and peroral steroid therapy was initiated. One month after starting steroids, a colovesical fistula was detected by follow-up CT. A colostomy and urethral catheterization were emergently performed. The patient recovered and the mass lesion was drastically minimized by the initiation of glucocorticoids; however, he still needs urethral catheterization. IgG4-RD develops in various systemic organs and generally responds well to steroids. Clinicians must be watchful for the complications of responses to corticosteroids, such as fistulization, when the mass lesion of IgG4-RD is adjacent to multiple luminal organs.

关键词： IgG4-related disease Intrapelvic mass Steroid therapy Colovesical fistula Colostomy Urethral catheterization

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Impact of friction stir welding on recrystallization of oxide dispersion strengthened ferritic steel

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Journal of Materials Science & Technology 2018年第1期34卷 209-213页

作者： Wentuo Han Pingping Liu Xiaoou Yi Qian Zhan Farong Wan Kiyohiro yabuuchi Hisashi Serizawa Akihiko Kimura School of Materials Science and Engineering University of Science and Technology Beijing. Beijing 100083. China Institute of Advanced Energy. Kyoto University Gokasho Uji Kyoto 611-0011Japan Joining and Welding Research Institute. Osaka University Ibaraki Osaka567-0047Japan

Oxide dispersion strengthened （ODS） steels can be used as the structural materials in the future fusion reactors and the fuel cladding materials in the advanced fission reactors. However, the weldability of ODS steels is a severe problem. In the present study, defect-free joints of the 15Cr-ODS ferritic steel were achieved by friction stir welding at different rotation speeds. The recrystallization, hardness and tensile properties are highly related with the rotation speed of the stir tool. The higher rotation speed results in coarser grains in the top SZ, while the grain size exhibits more complicated relation with the rotation speed in the SZ center. The joint welded at 250 rpm exhibits a maximum tensile strength of 974 MPa that reaches about 84% of that of the base metal.

关键词： ODS ferritic steel Friction stir welding Recrystallization behavior Electron backscatter diffraction Vickers hardness

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Efficient Stabilization of Na Storage Reversibility by Ti Integration into O′3-Type NaMnO_(2)

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Energy Material Advances 2021年第1期2021卷 143-154页

作者： Takuro Sato Kazuki Yoshikawa Wenwen Zhao Tokio Kobayashi Hongahally Basappa Rajendra Masao Yonemura Naoaki yabuuchi Department of Chemistry and Life Science Yokohama National University79-5 TokiwadaiHodogaya-kuYokohamaKanagawa 240-8501Japan Department of Applied Chemistry Tokyo Denki UniversityAdachiTokyo 120-8551Japan Elements Strategy Initiative for Catalysts and Batteries Kyoto Universityf1-30 Goryo-OharaNishikyo-kuKyoto 615-8245Japan Institute of Materials Structure Science High Energy Accelerator Research OrganizationTsukubaIbaraki 305-0801Japan Department of Materials Structure Science Sokendai(The Graduate University for Advanced Studies)203-1 ShirakataTokaiIbaraki 319-1106Japan Advanced Chemical Energy Research Center Yokohama National University79-5 TokiwadaiHodogaya-kuYokohamaKanagawa 240-8501Japan

The development of an energy storage system with abundant elements is a key challenge for a sustainable society,and the interest of Na intercalation chemistry is extending throughout the research ***,the impact of Ti integration into NaMnO2 in a binary system of x NaMnO2–(1–x)TiO2(0:5≤x≤1)is systematically examined for rechargeable Na battery *** NaMnO2,which is classified as an in-plane distorted O′3-type layered structure,delivers a large initial discharge capacity of approximately 200 mAhg-1,but insufficient capacity retention is observed,most probably associated with dissolution of Mn ions on electrochemical ***-substituted samples show highly improved electrode performance as electrode ***,the appearance of a sodium-deficient phase,Na4Mn4Ti5O18 with a tunnel-type structure,is observed for Ti-rich *** the samples in this binary system,Na0.8Mn0.8Ti0.2O2(x=0:8),which is a mixture of a partially Ti-substituted O′3-type layered oxide(Na0.88Mn0.88Ti0.12O2)and tunnel-type Na4Mn4Ti5O18 as a minor phase elucidated by Rietveld analysis on both neutron and X-ray diffraction patterns,shows good electrode performance on the basis of energy density and *** phases are electrochemically active as evidenced by in situ X-ray diffraction study,and the improvement of reversibility originates from the suppression of Mn dissolution on electrochemical *** these results,the feasibility of Mn-based electrode materials for high-energy rechargeable Na batteries made from only abundant elements is discussed in detail.

关键词： electrode system Storage

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Effects of laminin on hard tissue formation by bone marrow cells <i>in vivo</i>and <i>in vitro</i>

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Journal of Biomedical Science and Engineering 2014年第1期7卷 15-23页

作者： Masataka Yoshikawa Hideyuki Kakigi Takayoshi yabuuchi Hiroyuki Hayashi Department of Endodontics Osaka Dental University Osaka Japan

The effect of laminin on hard tissue formation using rat bone marrow cells was assessed. Rat bone marrow cells were obtained from femora of 6-week-old male Fischer 344 rats. In this in vivo examination, porous cylindrical hydroxyapatite scaffolds with a hollow center were immersed in 100 mg/ml laminin solution and air-dried. Rat bone marrow cells in 200 ml culture medium at 1 × 106 cells/ml were seeded in the scaffolds. The scaffolds were implanted into the dorsal subcutis of 7-week-old male Fischer 344 rats for 6 weeks. The scaffolds were then removed and examined histologically. For in vitro examinations, 1 × 105 rat bone marrow cells in 2 ml culture medium were then cultured with the addition of dexamethasone and laminin. Rat bone marrow cells were also cultured in laminin-coated culture plates. In vitro examinations showed the effectiveness of laminin for hard tissue formation from the results of biochemical and immunochemical analysis. From the in vivo examination, laminin coating of the scaffolds induced hard tissue in the pores with the cells. It is concluded that laminin is useful for bone formation, as in an in vitro culture study using bone marrow cells, in hydroxyapatite scaffolds in vivo.

关键词： Laminin Bone Marrow Cells Scaffold Hydroxyapatite Osteogenesis

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Hybrid Scaffolds Composed of Amino-Acid Coated Sponge and Hydroxyapatite for Hard Tissue Formation by Bone Marrow Cells

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Journal of Biomedical Science and Engineering 2014年第6期7卷 316-329页

作者： Takayoshi yabuuchi Masataka Yoshikawa Hideyuki Kakigi Hiroyuki Hayashi Department of Endodontics Osaka Dental University Osaka City Japan

A formalin-treated polyvinyl-alcohol (PVF) sponge is convenient as a scaffold because its configuration is easily modified. However, coating the sponge with an adhesive chemical agent is necessary to attach bone marrow cells (BMCs) to the sponge structure. Moreover, it was considered that a hybrid scaffold composed of a sponge and enveloped cylindrical porous hydroxyapatite (HA) would be convenient. In this study, the effect of leucine (Leu) coating on a PVF sponge was examined for osteogenesis on an HA/PVF hybrid scaffold by rat BMCs (rBMCs). In an in vivo assessment, the sponge immersed in Leu solution (10 mg/ml) was inserted into the hollow center of cylindrical HA. The sponge received 1.5 × 106 rBMCs obtained from male Fischer 344 rats. The hybrid scaffolds were then implanted subcutaneously of syngeneic rats for 6 weeks. In vitro assessment of Leu to hard tissue formation with coating on the well or addition in rBMC culture medium was also performed in a 6-well plate for 2 weeks. In vivo examinations showed the excellent effect of Leu coating on PVF sponge. Leu-coated PVF sponge in the scaffolds showed marked new bone formation in the pores by histological examination. Leu-coated PVF sponge showed a high quantity of osteocalcine (OC). HA might prevent the release of rBMCs from PVF as a barrier. In in vitro examinations, the quantity of OC in rBMC culture with and without the addition of Leu in culture medium showed no significant difference. However, addition of Leu showed significant ALP activity level in culture medium. Leu coating in culture plate wells showed no influence on the quantity of OC. It was concluded from the results that Leu might prevent the emigration of rBMCs to the outside of the scaffold and promote the differentiation of cells to osteoblasts in the scaffold.

关键词： Amino-Acid Bone Marrow Cells Bone Formation Hybrid Scaffold Scaffold Hydroxyapatite Formalin-Treated Polyvinyl-Alcohol Sponge

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Effect of sheath potential on electromagnetic radiation emitted from the rear surface of a foil target

Effect of sheath potential on electromagnetic radiation emit...

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2006年全国强场激光物理会议

作者： T.yabuuchi K.A.Tanaka Institute of Laser Engineerin Osaka University Institute of Laser Engineerin Osaka University

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Effect of L-lysine in culture medium on nodule formation by bone marrow cells

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Journal of Biomedical Science and Engineering 2012年第10期5卷 587-592页

作者： Masataka Yoshikawa Yasunori Shimomura, Hideyuki Kakigi Norimasa Tsuji Takayoshi yabuuchi Hiroyuki Hayashi Department of Endodontics Osaka Dental University Osaka Japan

The aim of this study was to estimate the effect of L-lysine on nodule formation by rat bone marrow cells in vitro. In this study, L-lysine was added to medium for mesenchymal stem cell culture to promote proliferation and differentiation of the cells, and then nodule formation was estimated in an in vitro rat bone marrow cell culture. Bone marrow cells from the bone shafts of the femora of Fischer 344 rats were cultured in minimum essential medium with 20 μl of L-lysine solution at 10﹣4, 10﹣5, 10﹣6, 10﹣7 or 10﹣8 M. Dexamethasone was also added to the medium at 10 nM for differentiation of stem cells from bone marrow into osteoblast progenitor cells. The subculture was performed for 2 weeks. The quantity of osteocalcin in rat bone marrow cell culture with dexamethasone was 392 ng/ml. In the medium with dexamethasone and 10﹣8 M L-lysine, the quantity of osteocalcin was 437 ng/ml. Nodules only formed upon addition of 20 μl of L-lysine at 10﹣8 M. It was indicated that 10﹣8 M L-lysine should be the optimal concentration for calcification. For nodule formation by rat bone marrow cells in vitro, the optimum concentration of L-lysine in culture medium might be 20 μl of 10﹣8 M. L-lysine could play an important role in matrix production for bone formation in vitro.

关键词： L-Lysine Bone marrow Cells Calcium Deposition Dexamethasone In Vitro

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Dextran coating on and among fibers of polymer sponge scaffold for osteogenesis by bone marrow cells in vivo

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Journal of Biomedical Science and Engineering 2010年第8期3卷 751-757页

作者： Masataka Yoshikawa Norimasa Tsuji Hideyuki Kakigi Takayoshi yabuuchi Yasunori Shimomura Hiroyuki Hayashi Hajime Ohgushi 不详

Although hydroxyapatite is commonly used as a scaffold for bone regeneration, sponges may be suitable because of the adaptability to the defect. To use as a scaffold, the fiber of sponge would be coated with any adhesive to storage stem cells in the sponges. Fiber in the structure of commercially available sponges was coated by immersion in dextran solution and air dried. After seeding of rat bone marrow cells (rBMCs), the sponges were implanted subcutis of rats for estimate osteogenesis in vivo. The level of osteocalcin was 25.28 ±5.71 ng/scaffold and that of Ca was 129.20 ±19.69 μg/scaffold. These values were significantly high- er than those in sponges without dextran coating (p <0.01). It was thought that rBMCs could be stored on the shelf by dextran deposition in the fiber of the sponge. In vivo examination, dextran induced osteogenesis by rBMCs in many spaces in the inner structure of the sponge.

关键词： Dextran Cell Adhesion Scaffold Bone Marrow Cells Osteogenesis

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Corrigendum to“Efficient Stabilization of Na Storage Reversibility by Ti Integration into O′3-Type NaMnO_(2)”

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Energy Material Advances 2021年第1期 13-14页

In the article titled“Efficient Stabilization of Na Storage Reversibility by Ti Integration into O′3-Type NaMnO2”[1],there was an error in Figure *** panel(a),the“x=0:2”should have read“x=0:8.”This is shown cor... 详细信息

关键词： Figure x=0 Efficient

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