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第二十届中国作物学会学术年会

作者： Yang Li Xiaolong Yang Zuolin Zhang yiyue lin Benfu Wang Zhisheng Zhang Changyan Liu Jianping Cheng Institute of Food Crops Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement College of Agriculture Yangtze University

Direct seeding rice(DSR) is a cost-saving and efficient production technology. It is of great significance to identify the characteristics of nitrogen demand of DSR in further improving yield and nitrogen use efficiency. In this study, a field experiment was carried out with inbred rice(HHZ) and hybrid rice(FLY4, YLY6) in Wuhan, China, in 2020 and 2021, respectively. Two fertilization ratios as follows: basal fertilizer: tillering fertilizer: panicle fertilizer = 5:2:3(T0);basal-tillering fertilizer: panicle fertilizer=6:4. Four first fertilization time set as: T1, basaltillering fertilizer was applied at 10 days after seeding(DAS);T2, basal-tillering fertilizer was applied at 15 DAS;T3, basaltillering fertilizer was applied at 20 DAS;T4, basal-tillering fertilizer was applied at 25 DAS. The changes of yield and yield components, above ground biomass accumulation, nitrogen use efficiency, harvest index and leaf SPAD were investigated. The results showed that the yield of DSR could be significantly increased by properly postponing the first basal-tillering fertilization time. Compared with the T0, the basal-tiller fertilizer application at 20 DAS could significantly increase the yield by 15.1%, mainly because the number of spikelets per unit area increased by 18.9 % on average across years and varieties. Delaying the first application time of basal-tillering fertilizer in DSR can significantly increase the biomass accumulation of aboveground leaves and stems and increase the yield, but with the decrease of harvest index in T3 and T4. In addition, delaying the first application time of basal-tiller fertilizer could also effectively improve the nitrogen use efficiency by 12.1% in DSR. The PFP and AE of T3 were significantly increased by 15.1% and 55.9% compared with T0, respectively. Delayed basal-tiller fertilizer treatment laid the foundation for maintaining higher leaf SPAD value in later developmental stage. To sum up, delaying the first application time of basal-t

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播期对水稻机械旱直播产量及稻米品质的影响

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核农学报 2022年第8期36卷 1648-1656页

作者：李阳杨晓龙汪本福张枝盛陈少愚林义月程建平湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室湖北武汉430064 长江大学农学院湖北荆州434023

为探明机械旱直播水稻在长江中游地区较优的播种时期,本试验以广两优476(GLY476)和黄华占(HHZ)两个水稻品种为材料,采用机械旱整旱播模式,设置5个播期处理,即S1(5月10日)、S2(5月17日)、S3(5月24日)、S4(5月31日)、S5(6月7日),考察播期... 详细信息

为探明机械旱直播水稻在长江中游地区较优的播种时期,本试验以广两优476(GLY476)和黄华占(HHZ)两个水稻品种为材料,采用机械旱整旱播模式,设置5个播期处理,即S1(5月10日)、S2(5月17日)、S3(5月24日)、S4(5月31日)、S5(6月7日),考察播期对水稻产量形成、光温资源利用率、稻米品质形成的影响。结果表明,广两优476和黄华占的产量均随着播期的延后呈先升高后降低的趋势,其中S4处理的产量最高,该处理下两水稻品种产量分别较S1高15.8%和27.4%。随着播期的延后,光能生产效率呈现逐渐增加的趋势,广两优476在S5处理下的光能生产效率最高,两年平均值为0.432 g·MJ^(-1),黄华占以S4处理最高,两年平均值为0.421 g·MJ^(-1);且两品种积温利用率的变化趋势和光能生产效率类似,均以S4处理最高,两者平均值分别为3.72和3.61 kg·℃^(-1)·d^(-1)·hm^(-2)。两品种在S4和S5处理下的整精米率均高于其他播期处理;随着播期延后,两品种S5处理的垩白粒率和垩白度分别较各自的最高值下降14.1~17.1个百分点和4.6~7.8个百分点;营养品质变化趋势和外观品质一致,随播期延后呈显著下降趋势,两品种S5处理的平均直链淀粉含量较S1处理降低1.3~1.5个百分点,蛋白质含量降低2.0个百分点。综上所述,长江中游地区机械旱直播水稻5月中上旬播种期使水稻遭遇高温危害,产量和稻米外观品质降低。将播期延迟到5月底至6月上旬,可有效避免高温危害,对提高水稻稳产能力具有重要作用。本研究为明确水稻生产区高温发生规律,调整适宜播种期,提高机械旱直播水稻稳产能力提供了重要参考。

关键词：机械旱直播播期产量稻米品质高温

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氮肥运筹对机直播水稻产量、品质及氮素利用率的影响

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华中农业大学学报 2023年第2期42卷 93-98页

作者：林义月李阳汪本福张枝盛杨晓龙张作林程建平湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室武汉430064 长江大学农学院荆州434025

为研究不同的氮肥运筹处理对机直播水稻的产量、稻米品质以及氮肥利用率的影响,在机械直播条件下,以南粳9108和黄华占为材料,在大田总氮量210 kg/hm^(2)的条件下,设置3种不同的氮肥运筹,基肥∶分蘖肥∶幼穗分化肥分别为N1(5∶2∶3)、N2(... 详细信息

为研究不同的氮肥运筹处理对机直播水稻的产量、稻米品质以及氮肥利用率的影响,在机械直播条件下,以南粳9108和黄华占为材料,在大田总氮量210 kg/hm^(2)的条件下,设置3种不同的氮肥运筹,基肥∶分蘖肥∶幼穗分化肥分别为N1(5∶2∶3)、N2(3∶4∶3)、N3(无∶6∶4),并以不施氮肥(N0)作为对照。结果显示,南粳9108和黄华占都在基肥∶分蘖肥∶幼穗分化肥为3∶4∶3时产量最高,分别为9 691.2、11 596.1 kg/hm^(2),与其他处理差异显著;稻米的糙米率、精米率、整精米率在处理间差异不显著,但直链淀粉、蛋白质含量随着穗肥比例的增加而升高,表现为N3>N2>N1,垩白率、垩白度随穗肥比例的增加而降低,表现为N1>N2>N3。水稻各个生育时期在3∶4∶3的处理下,氮素吸收显著高于其他处理并且氮素农学利用率、氮肥利用率表现为N2>N3>N1。研究结果表明,在施氮量相同的情况下,氮肥运筹N2(3∶4∶3)可显著提高水稻产量和氮素利用率,而N3(无∶6∶4)处理显著提高稻米营养品质和外观品质。

关键词：直播稻机直播氮肥运筹产量稻米品质氮素利用率

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Assessing the efficacy and safety of gemcitabine-induced thrombocytopenia/neutropenia and thrombocytopenia interventions in zebrafish

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Journal of Genetics and Genomics 2024年第9期51卷 970-973页

作者： Jialong Deng Ziyuan Zhou Wangjun Liao Qing lin yiyue Zhang Innovation Centre of Ministry of Education for Development and Diseases School of MedicineSouth China University of TechnologyGuangzhouGuangdong 510006China Department of Hematology The Sixth Affiliated HospitalSchool of MedicineSouth China University of TechnologyFoshanGuangdong 528200China Department of Oncology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515China

Cancer is a major global health issue;in 2020,there were 19.29 million new cancer cases and 9.96 million cancer deaths worldwide(Siegel et al.,2023).Despite ongoing research,chemotherapy remains the primary treatment due to its broad anti-cancer effects(Marra and Curigliano,2021).However,intravenous chemotherapeutic drugs commonly induce hematotoxicity,necessitating dose adjustments or treatment ***-induced thrombocytopenia(CIT)and neutropenia(CIN)are the primary manifestations of chemotherapy-induced hematotoxicity,which requires intervention due to the heightened risk of bleeding and infection(Blayney and Schwartzberg,2022).Currently,there are no FDA-approved treatments for CIT(Song and Al-Samkari,2023).While a CIT mouse model using 5-FU has been developed for drug evaluation(Shin et al.,2023),it lacks a comprehensive hematotoxicity assessment and the benefits of high throughput,cost-effectiveness,and ***,it is urgent to develop a high-throughput CIT model for risk assessment and safer chemotherapy strategies with clinical potential.

关键词： chemotherapy treatment visibility

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Large-Scale Forward Genetic Screening Analysis of Development of Hematopoiesis in Zebrafish

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Journal of Genetics and Genomics 2012年第9期39卷 473-480页

作者： Kun Wang Zhibin Huang lingfeng Zhao Wei Liu Xiaohui Chen Ping Meng Qing lin Yali Chi Mengchang Xu Ning Ma yiyue Zhang Wenqing Zhang Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes Department of Cell Biology School of Basic Medical Sciences Southern Medical University Guangzhou 510515 China

Zebrafish is a powerful model for the investigation of *** order to isolate novel mutants with hematopoietic defects, large-scale mutagenesis screening of zebrafish was *** scoring specific hematopoietic markers,52 mutants were identified and then classified into four types based on specific phenotypic *** mutant represented a putative mutation of a gene regulating the relevant aspect of hematopoiesis,including early macrophage development,early granulopoiesis,embryonic myelopoiesis,and definitive erythropoiesis/*** method should be applicable for other types of genetic screening in *** addition,further study of the mutants we identified may help to unveil the molecular basis of hematopoiesis.

关键词： Zebrafish Large-scale screening Hematopoiesis

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Zebrafish for thrombocytopoiesis-and hemostasis-related researches and disorders

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Blood Science 2020年第2期2卷 44-49页

作者： Panpan Meng Liangliang Wu Qing lin yiyue Zhang Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes Department of Developmental BiologySchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515P.R.China Division of Cell Developmental and Integrative BiologySchool of MedicineSouth China University of TechnologyGuangzhou 510006P.R.China

Platelets play vital roles in hemostasis,inflammation,and vascular *** are also active participants in the immune *** vertebrates,zebrafish have a highly conserved hematopoietic system in the developmental,cellular,functional,biochemical,and genetic levels with *** in zebrafish are functional homologs of mammalian ***,we summarized thrombocyte development,function,and related research techniques in zebrafish,and reviewed available zebrafish models of platelet-associated disorders,including congenital amegakaryocytic thrombocytopenia,inherited thrombocytopenia,essential thrombocythemia,and blood coagulation disorders such as gray platelet *** elegant zebrafish models and methods are crucial for understanding the molecular and genetic mechanisms of thrombocyte development and function,and provide deep insights into related human disease pathophysiology and drug development.

关键词： Animal models Platelet-associated diseases Thrombocytopoiesis Zebrafish

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DNA methylation regulation of gabrb2 mRNA expression disrupted in L-Methionine-induced zebrafish with schizophrenia-like symptoms

DNA methylation regulation of gabrb2 mRNA expression disrupt...

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中华医学会第十五次全国医学遗传学学术会议暨中国医师协会医学遗传医师分会第一届全国学术会议暨2016年浙江省医学遗传学年会

作者： Wang Lijuan Jiang Wei lin Qing Zhang yiyue Zhao Cunyou Department of Medical Genetics School of Basic Medical Sciences South Medical University Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes Department of Developmental Biology Institute of Genetic Engineering School of Basic Medical Sciences Southern Medical University

Objective In this study, a zebrafish animal model was used to evaluate the relationship between t he gabrb2 m RNA expression and its promoter DNA methylation in developmental and MET-induc ed schizophrenia-like zebrafish, in order to better understand the plausible epigenetic mechanism of GABAergic system underlying schizophrenia pathogenesis. Method Methylation dynamic pattern during zebrafish development was assayed with HpaⅡ/Ms pⅠ enzyme digestion followed by Fluorescence polarization based measurement of DNA methyl ation(FPDM) method for global genome, and quantitative real-time PCR(q PCR) method for gabr b2 specific promoter ***2 m RNA expression pattern during zebrafish development was assayed with q PCR method after total RNA reverse transcript into total c ***2 m RNA distr ibution during zebrafish central nervous system(CNS) development was assayed with Whole-mo unt in-situ hybridization(WISH).Three Behavioral tests performed,with 30 minutes pre-acclimation in the testing room. Novel tank test was for general mental status and locomotor activity measure ment. Social interaction test was for social preference judgment. T-maze tank test was for cogniti ve ability *** MET exposure, methylation changes of global genome was detected by FP DM and specific promoter regions of gabrb2, gad1 b, gad2 and slc6 a1 b was detected by q PCR. Result The results indicated developmental increases in global DNA methylation and decreases i n gabrb2 promoter methylation in zebrafish. A significant increase in gabrb2 m RNA levels was ob served after GABA was synthesized. Additionally, the MET-triggered schizophrenia-like symptom s in adult zebrafish, involving social withdrawal and cognitive dysfunction analyzed with social inte raction and T-maze behavioral tests, were accompanied by significantly increased DNA methylati on levels in the global genome and the gabrb2 promoter. Furthermore, the significant correlation between gabrb2 m RNA expression and gabr

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方坯二冷热应力分析

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热加工工艺 2010年第19期39卷 75-78页

作者：杨以月林晨赵小军青岛理工大学机械工程学院山东青岛266033

根据方坯连铸过程中在二冷区的凝固特点,应用ANSYS有限元软件建立了铸坯的数学模型,并模拟方坯凝固时的应力场分布情况,简要分析二冷热应力的变化对铸坯质量的影响,为研究二冷对铸坯的质量影响提供了理论依据。模拟结果表明:提高拉速,... 详细信息

根据方坯连铸过程中在二冷区的凝固特点,应用ANSYS有限元软件建立了铸坯的数学模型,并模拟方坯凝固时的应力场分布情况,简要分析二冷热应力的变化对铸坯质量的影响,为研究二冷对铸坯的质量影响提供了理论依据。模拟结果表明:提高拉速,内裂产生的可能性减小;提高比水量使偏角区热应力增大,对表面和角部热应力影响较小;和比水量相比较,拉速对热应力的影响比较大。

关键词：方坯 ANSYS 数学模型应力场内裂

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少年儿童图书馆阅读推广活动的多元参与研究

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国家图书馆学刊 2019年第6期28卷 31-41页

作者：王亦越黄琳李桂华四川大学公共管理学院

促进少儿阅读推广是全民阅读的重要工作。文章运用内容分析法,以我国29家少年儿童图书馆2019年"世界读书日"阅读推广活动的参与者为研究对象,从"人"和"机构"两个维度构建少儿阅读推广活动的多元参与体系。在此基础上,建议少儿图书馆在... 详细信息

促进少儿阅读推广是全民阅读的重要工作。文章运用内容分析法,以我国29家少年儿童图书馆2019年"世界读书日"阅读推广活动的参与者为研究对象,从"人"和"机构"两个维度构建少儿阅读推广活动的多元参与体系。在此基础上,建议少儿图书馆在阅读推广活动中应重视活动设计的针对性,扩大阅读推广的目标范围,建立信息交流反馈机制;注重多元合作与联合推广,构建阅读推广服务链,提高社会参与度;利用媒介优势互补,探索阅读推广新方法,打造多渠道融合的阅读推广服务平台。图1。表5。参考文献23。

关键词：少儿图书馆阅读推广参与人员合作单位多元参与

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Myeloperoxidase-deficient Zebrafish Show an Augmented Inflammatory Response to Challenge with Candida albicans

Myeloperoxidase-deficient Zebrafish Show an Augmented Inflam...

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第二届全国发育生物学大会

作者： Kun Wang Xiao Fang Ning Ma Qing lin Zhibin Huang Wei Liu Mengchang Xu Xiaohui Chen Wenqing Zhang yiyue Zhang Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes Department of Cell BiologySouthern Medical University

Myeloperoxidase is a key component of neutrophil granules involved in killing engulfed *** vitro experiments and mouse model studies have demonstrated that myeloperoxidase deficiency markedly reduces the microbicidal activity of neutrophils against several species of bacteria and ***,human myeloperoxidase deficiency has a relatively mild phenotype,implying unknown compensatory *** obtained a zebrafish mutant(smu681)lacking Sudan black staining by large-scale screening,which was a neutrophil-replete but myeloperoxidase-deficient *** contrast to findings in the mouse model,smu681 embryos and wild-type embryos showed similar survival after Candida albicans *** of *** was more rapid in smu681 embryos than in wild-type embryos,although it was eventually *** addition,the number of neutrophils accumulating at the site of infection was significantly larger in mutant embryos than in wild-type embryos,and mutant embryos showed increased expression of several inflammatory cytokines after *** *** findings indicate that myeloperoxidase deficiency alters the inflammatory response to fungal infection,presumably to compensate for impairment of neutrophil microbicidal activity.

关键词： myeloperoxidase,neutrophil immunity fungi infection

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