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Long non-coding RNA Tug 1 regulates inflammation in microglia and in status epilepticus rats through the NF-κB signaling pathway

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Acta Epileptologica 2022年第2期5卷 88-96页

作者： Ming Wang Yangmei Xie yiye shao Yinghui Chen Department of Neurology Huashan HospitalFudan UniversityShang-hai 200040China Department of Neurology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China.

Background:Inflammation plays an important role in the pathogenesis of status epilepticus(SE).The long non-cod-ing RNA(lncRNA)taurine up-regulated gene1(Tug1)plays a well-defned role in inflammatory ***,the molecular mechanism of Tug1 in SE progression remains *** present study,we investigated whether Tug1 is involved in microglial inflammation in SE ***:The SE rat model was established via intraperitoneal injection of lithium chloride ***-binding protein immunoprecipitation(RIP)and RIP sequencing were carried out in rat microglia(RM).Tug1 cloned into the adenovirus was overexpressed in the *** of Tug1 was performed via siRNA *** level of Tug1 and inflammatory factors IL-1βand TNF-αwas examined by real-time polymerase chain reaction(RT-PCR)and western *** levels of p65,P-p65,p-IKBa and IkBa were assessed by western ***:The RIP-seq result showed 14 lncRNAs that bound to the NF-κB p65 protein in *** lncRNA Tug1 directly interacted with *** level of declined Tug1 was decreased in the hippocampus of SE *** ofTug1 reduced the LPS-induced inflammation and M1/M2 polarization of microglia,while knockdown of Tug1 aggravated the inflammatory response in ***,the protein levels of p-p65/p65 and p-IkBa/IkBa were reduced in the Tug1-overexpression microglia and elevated in the Tug1-knockdown ***:These findings indicate that Tug1 modulates the inflammation in microglia through the NF-κB signal pathway,and the Tug1/P65 axis are like to play a signifcant role in the inflammatory processes,providing a valid target for the therapy of SE.

关键词： Inflammation Microglia Status epilepticus lncRNA NF-κB signaling pathway Tug1

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Evaluation of P-glycoprotein-targeting circulating microRNAs as peripheral biomarkers for medically intractable epilepsy

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Acta Epileptologica 2023年第1期6卷 14-24页

作者： Yangmei Xie yiye shao Xue Gong Ming Wang Yinghui Chen Department of Neurology Huashan HospitalFudan UniversityNational Center for Neurological Disorders12 Wulumuqi RoadShanghai 200040China Department of Neurology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200940China Community Health Service Center of Xinqiao Town Songjiang DistrictShanghai 201612China

Background Early diagnosis of medically intractable epilepsy is challenging in clinical work.P-glycoprotein(P-gp)is one of the most important multidrug efux transporters,which has been demonstrated to contribute to the drug resistance of intractable *** present study was aimed to explore the diagnostic value of microRNAs(miRNAs)targeting P-gp for medically intractable *** Thirty-six patients with intractable epilepsy and 36 epilepsy patients responsive to anti-epilepsy drugs,who visited Jinshan Hospital of Fudan University from September 2014 to September 2016,were enrolled in this *** information of the patients was obtained by retrospectively reviewing medical *** with diferential serum expression between the two groups of patients were detected by microarray ***,miRNAs that were confrmed to regulate P-gp in vitro by western blot were selected for further *** the validation phase,reverse transcription quantitative PCR(RT-qPCR)was conducted to confrm the diferential expression of the candidate miRNAs in the epilepsy *** operating characteristic(ROC)curve analysis was carried out to evaluate the diagnostic value of the miRNAs for intractable *** Three miRNAs including miR-6514-3p,miR-6076-5p,and miR-6855-3p were identifed to be candidate miRNAs by microarray *** results of western blotting validated that miR-146a-5p and miR-138-5p could regulate P-gp expression in vitro,so they were included in the candidate miRNAs for further *** the validation phase,the results of RT-qPCR indicated that compared with drug-responsive patients,the patients with intractable epilepsy showed decreased level of miR-138-5p and increased level of *** results of ROC curve analysis indicated that miR-138-5p(AUC=0.877)and miR-146a-5p(AUC=0.866)had high diagnostic value for intractable *** addition,the miR-panel composed of miR-138-5p and miR-146a-5p showed higher di

关键词： Intractable epilepsy Drug resistance P-glycoprotein Circulating miRNAs Biomarker

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Combining the first class with second class to improve the professional skills of medical students

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重庆医学 2013年第23期42卷 2810-2812页

作者： Ni Yanbo Ji Piyou Wang Chunyan Zhang Lixia shao yiye Luan Xiying Binzhou Medical University College of Basic Sciences Rushan Center for Disease Control and Prevention Binzhou Medical University Medical Center

Medical science is a highly practical subject,which is closely related to human *** institutions take a heavy social responsibility,in addition to the sacred mission of teaching and *** professional skills of medical students are one important assessment indicators to become a qualified ***,how to improve the professional skills of medical students in their training process becomes crucial.

关键词：医疗机构医疗卫生行业专业技能训练方法

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Analysis and countermeasures of the conflicting between clinical practice and taking test of postgraduate of medical undergraduates

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重庆医学 2014年第25期43卷 3393-3396页

作者： Zhang Lixia Gu Yun Cai Heng Liang shaohua shao yiye Ni Yanbo Medicine and Pharmacy Research Center Binzhou Medical University Penglai City People's Hospital Basic School of Medicine Binzhou Medical University Shanghai Medical College of Fudan University

Nowadays,with the expanding enrollment of medical schools in our country,the competitive pressures of employment *** is very difficult for the graduates of these schools to find ideal jobs,but medical students with master or doctor degrees are relatively optimistic to the employment *** a result,more and more fresh medical graduates pursue the post-graduate education,thus forms a"rush of taking test of postgraduate"phenomenon[1].Clinical students

关键词：医学教育教育学医学研究临床实验

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microRNA-146a在LPS诱导的大鼠雪旺细胞损伤模型中的表达

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中国临床医学 2015年第3期22卷 288-290页

作者：周秀梅陈龙邵一叶陈英辉施晓红复旦大学附属金山医院神经内科上海201508 复旦大学附属金山医院内分泌科上海201508

目的:探讨microRNA-146a(miRNA-146a)在体外培养的大鼠雪旺细胞炎性反应模型中的表达变化。方法:采用脂多糖(lipopolysaccharide,LPS)建立大鼠雪旺细胞炎性反应模型(LPS组),加入LPS 6 h后给予环氧合酶-2(COX-2)抑制剂NS398处理,正常组仅... 详细信息

目的:探讨microRNA-146a(miRNA-146a)在体外培养的大鼠雪旺细胞炎性反应模型中的表达变化。方法:采用脂多糖(lipopolysaccharide,LPS)建立大鼠雪旺细胞炎性反应模型(LPS组),加入LPS 6 h后给予环氧合酶-2(COX-2)抑制剂NS398处理,正常组仅在LPS组加入LPS时更换培养基。采用real-time PCR及CCK-8方法检测miRNA-146a的表达水平及雪旺细胞的增殖能力。结果:与正常组相比,LPS组miRNA-146a的表达明显升高(P<0.05),而且miRNA-146a的表达随LPS浓度升高而增加;经1.0μg/mL的LPS处理6 h后给予NS398处理,miRNA-146a表达水平下降(P<0.05)。经10μg/mL的LPS处理6 h后,雪旺细胞的增殖能力下降;而LPS处理6 h后加入NS398,雪旺细胞的增殖能力改善。结论:miRNA-146a参与了体外培养的大鼠雪旺细胞炎性反应的调控,其表达水平在一定程度上可反应炎性反应的严重程度。

关键词：雪旺细胞 miRNA-146a 炎性反应

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城轨车辆制动防滑检测与保护

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机车电传动 2015年第2期 94-98页

作者：余接任钱华夏帅邵跃虎李一叶株洲南车时代电气股份有限公司湖南株洲412001

分析了城轨车辆制动防滑系统常用的速度差、减速度等滑行检测判据的弊端,对速度差中车辆参考速度的计算和减速度检测灵敏度太高提出改进建议,对比分析2种不同防滑策略恢复时机的选择对防滑效率及参考速度的影响,结合两者优势,给出一种... 详细信息

分析了城轨车辆制动防滑系统常用的速度差、减速度等滑行检测判据的弊端,对速度差中车辆参考速度的计算和减速度检测灵敏度太高提出改进建议,对比分析2种不同防滑策略恢复时机的选择对防滑效率及参考速度的影响,结合两者优势,给出一种较优的防滑策略。

关键词：城轨车辆制动防滑系统滑行检测速度差参考速度滑移率

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